Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT98.8% 245.1 / ‑4.8 80T15T (ACA→ACTUSA300HOU_2392FemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (31/48);  total (32/48)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCAC  >  CP000730/2532162‑2532434
                                                                                                                                        |                                                                                                                                        
gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTActacaa                                                                                                                                      <  1:474904/141‑1 (MQ=255)
 tgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                     >  1:748150/1‑141 (MQ=255)
 tgtAGTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGATATTTGTAACGTTGGCTTCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                     >  1:550958/1‑141 (MQ=255)
  gtAGTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                    <  1:401625/141‑1 (MQ=255)
        gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                              <  1:241952/141‑1 (MQ=255)
         tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                             <  1:271509/141‑1 (MQ=255)
          aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCGAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                            <  1:753486/141‑1 (MQ=255)
           aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTca                                                                                                                           >  1:19034/1‑141 (MQ=255)
           aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATGATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcc                                                                                                                           >  2:539118/1‑140 (MQ=255)
              aatttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                        <  2:159290/139‑1 (MQ=255)
              aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                        <  1:742831/141‑1 (MQ=255)
              aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCACAGAGGGATGGGATAAAATGAACTTGGTAACGTTGACTTCGGATGAGTTCAGTGCGTTTGCTACAAAGCATTTTTCACAt                                                                                                                        <  1:524083/141‑1 (MQ=255)
              aGTGTTTGATAAAATAAAAAAATAGCATTAGTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                        <  2:716812/141‑1 (MQ=255)
                tttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATCGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTa                                                                                                                      <  2:762764/141‑1 (MQ=255)
                    tGATAAAATAAAAACATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                  >  1:425023/1‑141 (MQ=255)
                    tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                  >  1:413550/1‑141 (MQ=255)
                    tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                  >  2:796335/1‑141 (MQ=255)
                     gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCAAATTacacaa                                                                                                                 >  1:293614/1‑140 (MQ=255)
                      ataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAcacaca                                                                                                                >  2:649062/1‑141 (MQ=255)
                       aaaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGTGGTATCCGTTTAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa                                                                                                               <  1:588215/140‑1 (MQ=255)
                       aaaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa                                                                                                               <  2:115928/140‑1 (MQ=255)
                       aaaaataaaaAAACAGTATTATTAAAAAATACAAAAATTATAATTCAATGCAGAGAGGGATGGGATAAAAAGAACTTTGTAACGTTGACTTCGGATAAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa                                                                                                               <  2:451339/140‑1 (MQ=255)
                         aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATc                                                                                                             >  2:202900/1‑141 (MQ=255)
                          aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                            >  1:479051/1‑141 (MQ=255)
                          aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATGCAGGTAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                            >  1:427522/1‑141 (MQ=255)
                           aaaaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAACGATGGGATAAAATGAACTTTGTAACGTTTACTTCGCATGAGTTCAATGCGTTTCCTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                           <  2:209142/139‑1 (MQ=255)
                             aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                         >  2:261963/1‑141 (MQ=255)
                               aaaaaTAGCATTACTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt                                                                                                       <  1:359950/141‑1 (MQ=255)
                                aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                      <  1:126939/141‑1 (MQ=255)
                                aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                      <  2:720313/141‑1 (MQ=255)
                                      gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc                                                                                                >  1:683741/1‑141 (MQ=255)
                                             taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCCGCTATTCATTACAATCaaa                                                                                         >  2:345961/1‑139 (MQ=255)
                                             taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGATTACTACAAAGCATTTTTCACATTACACACAATCGGCTATTACTTACCataata                                                                                         >  2:18765/1‑141 (MQ=255)
                                               ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATAAGCTATTCATTACAATAATagg                                                                                       >  2:200579/1‑140 (MQ=255)
                                                    ttCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGa                                                                                  >  2:384755/1‑141 (MQ=255)
                                                         aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                             <  1:400868/141‑1 (MQ=255)
                                                          aaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaa                                                                            <  1:743356/141‑1 (MQ=255)
                                                          aaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaa                                                                            <  1:405836/141‑1 (MQ=255)
                                                           aTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaaa                                                                           <  1:770926/141‑1 (MQ=255)
                                                            ttATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAg                                                                          <  2:683741/141‑1 (MQ=255)
                                                                  ttCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                    <  1:575911/141‑1 (MQ=255)
                                                                        tcaagcagGGATGGGATAAAATGAACTTTGTACCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                              <  2:162073/135‑1 (MQ=255)
                                                                        tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                              <  2:418969/141‑1 (MQ=255)
                                                                        ccAGATAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                              <  2:549964/140‑1 (MQ=255)
                                                                         cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGCACTGATTTAAATGACGATGTTCAttt                                                             >  2:760527/1‑139 (MQ=255)
                                                                          agagatGGATGGGATAAAATAAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                            <  2:658134/141‑1 (MQ=255)
                                                                          agagagGGATGGGATAAAATGAACTTTGTATCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAATGGCGATGTGCATCtt                                                            <  2:648812/141‑1 (MQ=255)
                                                                          agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                            <  2:597982/141‑1 (MQ=255)
                                                                          agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGACGATGTGCATCtt                                                            >  1:615869/1‑141 (MQ=255)
                                                                           gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                           >  1:486702/1‑141 (MQ=255)
                                                                             gagggtatggatAAAATGAGCTTTGTAACGTTGACTTCGGATGAGTTCAATGCTTCTCCTCCAATGCACTTATCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAATGCTATGTGCATCTTGTa                                                         <  2:87219/133‑1 (MQ=255)
                                                                             gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTATTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTAATTTAAAAGGCGATGTGCATCTTGTa                                                         >  1:496402/1‑141 (MQ=255)
                                                                                   tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                   <  1:384755/141‑1 (MQ=255)
                                                                                             tGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACa                                         <  1:808425/141‑1 (MQ=255)
                                                                                              gAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa                                        <  1:250068/141‑1 (MQ=255)
                                                                                              gAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGACGATGTGCATCTTGTAGGGGTTAAAGATGACaa                                        >  2:83323/1‑141 (MQ=255)
                                                                                              gAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATCTTTCACATTACACACAATCAGTTCTTCATTACAATCATTGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa                                        <  2:489505/141‑1 (MQ=255)
                                                                                                     gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCaa                                 <  1:649062/141‑1 (MQ=255)
                                                                                                     gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCaa                                 <  2:620257/141‑1 (MQ=255)
                                                                                                     gTAACGTTGACTTCCGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCaa                                 <  1:648505/141‑1 (MQ=255)
                                                                                                      tAACGTTGACTTCGGCTGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAg                                <  1:202900/141‑1 (MQ=255)
                                                                                                          gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                            >  2:413334/1‑141 (MQ=255)
                                                                                                           ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                           >  1:312537/1‑141 (MQ=255)
                                                                                                            tGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg                          <  2:738336/141‑1 (MQ=255)
                                                                                                            tGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTACGGGTTAAAGATGACAATGGTCAAGTGATTg                          <  2:804967/141‑1 (MQ=255)
                                                                                                              aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                        >  2:394295/1‑141 (MQ=255)
                                                                                                                ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                      >  1:40470/1‑141 (MQ=255)
                                                                                                                ttCGGATGAGATCAATGCGTTTACAACAAAGCATTTTTCACATTACAAACAACGAACGATTCATTACCATCAGAGATTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                      >  1:591498/1‑141 (MQ=255)
                                                                                                                 tCGGTTGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGa                     <  1:309415/141‑1 (MQ=255)
                                                                                                                 tCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACTATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGa                     <  1:712501/141‑1 (MQ=255)
                                                                                                                  ggggttaGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                    <  1:481825/135‑1 (MQ=255)
                                                                                                                    gatgatTTCAATGCGTATCCTACAAAGCATTTTTCACATTACCCACAATCAACTATTCATTACAATCATAGAGTTGATTGAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGc                  <  2:797423/141‑1 (MQ=255)
                                                                                                                       gAGTTCAATGGGTTTACTGCAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAACGGTCAAGTGATTGCAGGATGCTTa               <  1:393734/141‑1 (MQ=255)
                                                                                                                         gTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt             >  2:711779/1‑141 (MQ=255)
                                                                                                                           ttaatccttttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGTCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa           <  2:738405/133‑1 (MQ=255)
                                                                                                                           cgaaTGCGTTTAATACAAACCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAACGGCGATGTGCATCTTGTAGGGGTTAAAGACGACAATGGTCAAGTGATTGCAGGATGCTTATTGa           <  2:441901/139‑1 (MQ=255)
                                                                                                                            taaTGCTTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGCCAAGTGATTGCAGGATGCTTATTGAc          <  1:200579/140‑1 (MQ=255)
                                                                                                                                gCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGTATGCTTATTGACAGaa      >  1:670509/1‑141 (MQ=255)
                                                                                                                                gCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGaa      >  1:747883/1‑141 (MQ=255)
                                                                                                                                   tttACTACAAAGCATTTTTCAAATGGCACACAATCAGCTATTCATTACAATCATAGAGTTAATTTCAAACACGATCTGCATCCTGTAGGGGAGAAGGACGACACTGGTAAAGTGATGGCAAGATGCTTAATGCTAGAAgc  >  2:388687/1‑140 (MQ=255)
                                                                                                                                    ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcac  <  2:668884/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCAC  >  CP000730/2532162‑2532434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: