Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,857,614 T→G 5.5% intergenic (+10/+55) USA300HOU_2699 → / ← USA300HOU_2700 NiCoT family nickel (Ni2+)‑cobalt (Co2+) transporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,857,6140TG 5.5% 244.8 / 2.4 83intergenic (+10/+55)USA300HOU_2699/USA300HOU_2700NiCoT family nickel (Ni2+)‑cobalt (Co2+) transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/44);  new base G (2/3);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGGGCATTCATTGGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTATTTAAAATGT  >  CP000730/2857476‑2857754
                                                                                                                                           |                                                                                                                                            
tGGGCATTCATTGGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCt                                                                                                                                            >  2:246568/1‑141 (MQ=255)
 gggCATTCATTGGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCtt                                                                                                                                           >  2:511223/1‑141 (MQ=255)
  ggCATTCATTGGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTg                                                                                                                                          >  1:681619/1‑141 (MQ=255)
     tttcattGGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCaa                                                                                                                                       <  2:320577/140‑1 (MQ=255)
     attcattGGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCaa                                                                                                                                       <  1:96232/141‑1 (MQ=255)
      ttcattGGTTCGATCCAATTTGATTACTCAGGCGATATTTTAGTGGCATTATTTTTAATTACTTGGCTGATTTCAAGTTTAATTTGCAAGTTTGGTCGTATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAAc                                                                                                                                      <  1:239742/141‑1 (MQ=255)
            ggTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCtt                                                                                                                                >  2:397851/1‑141 (MQ=255)
              ttCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTg                                                                                                                              <  1:236175/141‑1 (MQ=255)
                    cgaATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTAttgt                                                                                                                        <  1:465642/141‑1 (MQ=255)
                    cgaATTTGATTATTTAGGCTATATCTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTAttgt                                                                                                                        <  1:51149/141‑1 (MQ=255)
                     gaATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTAttgtt                                                                                                                       <  2:593296/141‑1 (MQ=255)
                     ccaatttcgtattTATGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTAttgtt                                                                                                                       <  2:164871/132‑1 (MQ=255)
                             ttattTAGGCTATATTTTAGTTTCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCAttttt                                                                                                               <  2:152633/141‑1 (MQ=255)
                              tattTAGGCTATATTTTCGTTGCTTGGTTTGTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCAtttttt                                                                                                              >  1:194608/1‑140 (MQ=255)
                              tattTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTa                                                                                                              >  1:207401/1‑141 (MQ=255)
                              tattTAGGCTATATTTTAGTTGCATTATTTTTAATTACATGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTa                                                                                                              >  1:520016/1‑141 (MQ=255)
                                tttAGGCTATATTTTTGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGa                                                                                                            <  2:466138/141‑1 (MQ=255)
                                tttAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGa                                                                                                            <  2:720871/141‑1 (MQ=255)
                                 ttAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTAATTCAGGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAGTGGTCTAGATAAGGCAACTTGTCTTGCAACTCCCTTTGTATTGTTGCAtttttcgcc                                                                                                           >  1:228781/1‑137 (MQ=255)
                                  tAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGATGTTTGGTCGCATAGAACACAAATGGTCTCGATAAGGCAACTTGTCTTGCAACTACCTTTGTCTTGTTGCATTTTTAGaca                                                                                                          >  2:604532/1‑141 (MQ=255)
                                  tAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGaca                                                                                                          >  1:699608/1‑141 (MQ=255)
                                  tAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGaca                                                                                                          <  1:427293/141‑1 (MQ=255)
                                  tAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGaca                                                                                                          <  2:158238/141‑1 (MQ=255)
                                  tAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCAAGATAAGGCTACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGacc                                                                                                          >  2:604529/1‑140 (MQ=255)
                                   aGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGacac                                                                                                         <  1:805677/141‑1 (MQ=255)
                                     gCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACAccc                                                                                                       <  1:68163/141‑1 (MQ=255)
                                     gCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACAccc                                                                                                       <  1:125716/141‑1 (MQ=255)
                                       tataTTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGTAGTTTGATCGCATAGAACATATAGTGCTTGTTGAACGCTACGTGTCTTGCATCGACCTTTGTTTTGTTGCATTTTTAGACACCCtt                                                                                                     >  2:170171/1‑140 (MQ=255)
                                         ttttttAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGca                                                                                                   <  2:634837/139‑1 (MQ=255)
                                           ttttAGTTGCATTAGTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGcaca                                                                                                 <  2:22266/141‑1 (MQ=255)
                                           tttctgTTGCGTTATGTTTACTGTCCTGCCTTATTTCAAGCTTCATTTGGCAGTCTCGTCGCATAGAACACACATGGTCTAGATACGGCAACTTGTCTTGCAACTAGCTTTGTATTCCTGCATTTTTAGTCACCCTGcaca                                                                                                 <  2:4079/136‑1 (MQ=255)
                                            tttAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGCCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCacac                                                                                                <  2:309185/141‑1 (MQ=255)
                                                         tttttAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACAACCTGCACACTTCTTCCATTATg                                                                                   >  1:94728/1‑141 (MQ=255)
                                                             tAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTAc                                                                               >  1:239653/1‑141 (MQ=255)
                                                              aaTTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGTTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACTCTTCTTCCATTATGCTACg                                                                              <  1:526507/141‑1 (MQ=255)
                                                              aaTTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGTTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACGCTTCTTCCATTATGCTACg                                                                              <  1:526627/141‑1 (MQ=255)
                                                                  aCTTAGCTTGTTTCAAGTTTAATTTGGAAGTTTGGTCGCATGGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCGACTACCTATGTATTGTTGCATTGTTAGACACCCTGCACACTTCTTCCATTAGGCTACGctcc                                                                          >  2:715176/1‑140 (MQ=255)
                                                                   cTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCtctc                                                                         <  2:490365/141‑1 (MQ=255)
                                                                      ggCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCagt                                                                      <  1:654064/141‑1 (MQ=255)
                                                                      ggCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGGCGTGGAACTACCTTTGTATGGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCagt                                                                      <  1:388957/141‑1 (MQ=255)
                                                                       gCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCagta                                                                     <  1:397851/141‑1 (MQ=255)
                                                                           aTTTCAGGTCTAATTTGGAAGTTTGATCGCATAGAACCGAAATGGTCGAGATAAGTCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCTTTATGCTACGCTCTCAGTAGTCa                                                                 <  2:667577/141‑1 (MQ=255)
                                                                             ttCAATTTTAATTTGTAAGTTTTGTCGCATAGAACATAATTGGTCGAGGTAAGGCAACTTGTTTGGCGACTACCTTTGTATTGTTGCATTGTTAGACACCGTGCACACGTCTTCCATTATGCTACGCTCTCAGTAGTCATc                                                               <  2:321241/141‑1 (MQ=255)
                                                                             ttCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATc                                                               <  2:321345/141‑1 (MQ=255)
                                                                             ttCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGGCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATc                                                               >  2:318158/1‑141 (MQ=255)
                                                                             ttCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGGCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATc                                                               >  2:798893/1‑141 (MQ=255)
                                                                              tCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCt                                                              >  2:798848/1‑141 (MQ=255)
                                                                              tCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTAAGTAGTCATCt                                                              >  1:135159/1‑141 (MQ=255)
                                                                               cAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCtt                                                             <  2:146001/141‑1 (MQ=255)
                                                                                 aGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTa                                                           >  1:412881/1‑141 (MQ=255)
                                                                                    ttAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAAt                                                        <  2:465556/141‑1 (MQ=255)
                                                                                    ttAAGTTGGAAGTTTGGTCGCATTGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAGAt                                                        <  2:359669/141‑1 (MQ=255)
                                                                                     tAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATc                                                       >  1:436911/1‑141 (MQ=255)
                                                                                      aaTTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAAtag                                                      >  2:599685/1‑139 (MQ=255)
                                                                                      aaTTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCg                                                      >  2:207161/1‑141 (MQ=255)
                                                                                            gAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCtt                                                >  2:31509/1‑141 (MQ=255)
                                                                                             aaGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCttt                                               <  2:748143/141‑1 (MQ=255)
                                                                                             aaGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCttt                                               <  1:464772/141‑1 (MQ=255)
                                                                                               gTTTGGGCGCATAGAACACGAATGGTTTAGATAAGGCATCTTGTCTTGCAATGACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACTCTCTTAGTATTCATCTTTAAATCGTTTCTTTTa                                             <  2:535408/141‑1 (MQ=255)
                                                                                                 ttGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTaga                                           >  1:329116/1‑141 (MQ=255)
                                                                                                         aTAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACtt                                   >  2:261409/1‑141 (MQ=255)
                                                                                                         aTAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACtt                                   <  1:246568/141‑1 (MQ=255)
                                                                                                            gAACACCAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCAt                                <  1:739236/141‑1 (MQ=255)
                                                                                                                 caAATGGTCTAGATAAGGCAACTTGGCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTga                           <  1:798893/141‑1 (MQ=255)
                                                                                                                 caAATGGTCTAGATAAGGCAACTTGGCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCCTTAAATCGTTTCTTTTAGAGATGACTTCATTTTga                           <  1:318158/141‑1 (MQ=255)
                                                                                                                  aagttgTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTgag                          <  2:261631/136‑1 (MQ=255)
                                                                                                                         cTAGATAAGGCAACTGGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTAt                   >  1:600171/1‑141 (MQ=255)
                                                                                                                            gATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTAtttt                >  2:703110/1‑141 (MQ=255)
                                                                                                                                 aGGCAACTTTTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTa            <  1:478529/141‑1 (MQ=255)
                                                                                                                                 aGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCtt            >  1:280828/1‑140 (MQ=255)
                                                                                                                                 aGGCAACTTGTCTCTCAACTACCTTTGTATTGGTGCATTTTTAGACACCCTGCTCACTTCTTCCAGTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTa            <  1:699233/141‑1 (MQ=255)
                                                                                                                                  ggCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTAt           <  1:511223/141‑1 (MQ=255)
                                                                                                                                  ggCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGCCCCCACGGCACCGGGGCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTAt           >  1:789465/1‑141 (MQ=255)
                                                                                                                                  ggCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTATCACTAGTCATCATTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTAt           >  1:385280/1‑141 (MQ=255)
                                                                                                                                  ggCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACACTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCGTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTAt           >  2:518959/1‑141 (MQ=255)
                                                                                                                                   gtaaCTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTAtt          <  2:93423/139‑1 (MQ=255)
                                                                                                                                    ctaCTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTAttt         <  1:275169/139‑1 (MQ=255)
                                                                                                                                    caccTTGTCTTGCAGCTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTAttt         <  1:809198/138‑1 (MQ=255)
                                                                                                                                    cAACTTGTCCTGCAACTACCTTTGTACTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTAttt         <  2:785245/141‑1 (MQ=255)
                                                                                                                                      aCTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTATTTaa       >  2:13728/1‑141 (MQ=255)
                                                                                                                                      aCTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTATTTaa       >  2:251972/1‑141 (MQ=255)
                                                                                                                                      aCTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGAACCCTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTATTTaa       >  1:114217/1‑141 (MQ=255)
                                                                                                                                          gTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTATTTAAAAt  >  2:481558/1‑140 (MQ=255)
                                                                                                                                           tCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGTGAGCGTATTTTTCTATTTAAAATGt  >  2:263875/1‑141 (MQ=255)
                                                                                                                                           tCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTCCATTTTGAGAGCGTATTTTTCTATTTAAAATGt  >  2:386031/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TGGGCATTCATTGGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAAGGCAACTTGTCTTGCAACTACCTTTGTATTGTTGCATTTTTAGACACCCTGCACACTTCTTCCATTATGCTACGCTCTCAGTAGTCATCTTTAAATCGTTTCTTTTAGAGATGACTTCATTTTGAGAGCGTATTTTTCTATTTAAAATGT  >  CP000730/2857476‑2857754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: