Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,909,490:1 +T 100% coding (297/303 nt) USA300HOU_1771 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,909,4861.T68.6% 155.6 / 76.3 70coding (293/303 nt)USA300HOU_1771hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base . (12/10);  new base T (28/20);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 7.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TTTGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCATAACTATG  >  CP000730/1909346‑1909620
                                                                                                                                              |                                                                                                                                        
tttGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCgg                                                                                                                                           <  1:196133/141‑1 (MQ=255)
 ttGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCCGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGt                                                                                                                                         >  1:329821/1‑141 (MQ=255)
 ttGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGCTTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGt                                                                                                                                         >  2:458280/1‑141 (MQ=255)
 ttGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGGACATATCTTTTCAATTGTTCGTACATTCGGt                                                                                                                                         >  1:278805/1‑141 (MQ=255)
  tGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGtt                                                                                                                                        <  1:121378/141‑1 (MQ=255)
  tGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGtt                                                                                                                                        <  1:486156/141‑1 (MQ=255)
     cTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAGTTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGttttt                                                                                                                                    >  2:54448/1‑140 (MQ=255)
     cTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGttttt                                                                                                                                    >  1:35661/1‑140 (MQ=255)
     cTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGttttt                                                                                                                                    <  1:604777/141‑2 (MQ=255)
     cTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGttttt                                                                                                                                    >  2:599058/1‑140 (MQ=255)
      tataATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGTAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGttttta                                                                                                                                   <  2:215105/141‑3 (MQ=255)
      tataATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTTTTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGttttta                                                                                                                                   >  1:59089/1‑139 (MQ=255)
       ataATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGtttttat                                                                                                                                  >  2:379698/1‑138 (MQ=255)
          ttttttGCAGGTTGTTCAATTAAATCACTAAAGCCTGATGGAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTtttattta                                                                                                                               <  2:373393/140‑1 (MQ=255)
          aTTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTtttattta                                                                                                                               <  1:440064/141‑1 (MQ=255)
               tGCAGATCGTTCAATTAAATCACTGAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTaaaaaa                                                                                                                          >  1:554344/1‑141 (MQ=255)
               tGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTaaaaaa                                                                                                                          >  2:106863/1‑141 (MQ=255)
               tGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTaaaaaa                                                                                                                          >  2:322590/1‑141 (MQ=255)
                gCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACTTTCGGTTTTTATTTAAAAAAg                                                                                                                         >  2:431648/1‑141 (MQ=255)
                gCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAg                                                                                                                         >  1:138342/1‑141 (MQ=255)
                 ccgATCGTTGAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCTTGGTGTATTTAAAAAAGt                                                                                                                        <  2:461248/139‑1 (MQ=255)
                   gATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCGTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTa                                                                                                                      >  1:587090/1‑141 (MQ=255)
                      cGTTCAATTAAATCACTAAAGCCTGATAAAGATTGGATTAGAATTGCGAGTAAAAGTTCGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCTGTTTTTATTTAAAAAAGTTAata                                                                                                                   >  2:183960/1‑141 (MQ=255)
                         tCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGt                                                                                                                >  1:3840/1‑141 (MQ=255)
                          cAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCa                                                                                                              >  2:545592/1‑141 (MQ=255)
                          cAATTAAATCACTAAAGCCTGATGCAGATTGCATTAGAATTGCGAGTAAAAGTTGGCCTTGGATTATTCTAATTGTTATTTTAGTACGTATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTCAAAAAGTGAatgtttt                                                                                                               >  2:294681/1‑136 (MQ=255)
                          aaaTTAAATCACTAAAGCCTGATGCAGATTAGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCa                                                                                                              <  1:27104/140‑1 (MQ=255)
                           aaTTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGATTTTATTTAAAAAAGTTAATATGTCAt                                                                                                             >  2:672148/1‑141 (MQ=255)
                               taaTCACTAAAGCCTGCTGGAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTTTAATTGTTATTTTAGTACATCTCTTTTCATTGGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGt                                                                                                          <  2:497327/140‑1 (MQ=255)
                                aaTCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTATTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGtg                                                                                                         >  1:533532/1‑140 (MQ=255)
                                   cACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGcc                                                                                                      <  1:375661/141‑1 (MQ=255)
                                    aCTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCTTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                    <  2:148390/141‑1 (MQ=255)
                                     cTAATGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAGAGTTAATATGTCATTGTAGCCtt                                                                                                    >  2:173631/1‑141 (MQ=255)
                                     cTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                    >  2:73908/1‑141 (MQ=255)
                                     cTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                    <  1:208412/141‑1 (MQ=255)
                                     cTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                    >  2:73905/1‑141 (MQ=255)
                                     cTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                    <  2:379332/141‑1 (MQ=255)
                                            cTGATGCAGATTGGATTAGAATTGCGAGTAAACGTTTGCCTTGGTTTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACaaa                                                                                            <  1:47403/141‑1 (MQ=255)
                                             tGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACaaa                                                                                            <  1:125541/141‑1 (MQ=255)
                                              gATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTGTTTGTAGCCTTAATACaaatc                                                                                          >  2:458601/1‑139 (MQ=255)
                                               aTGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAATAAAGTTAATATGTCATTGTAGCCTTAATACAAAac                                                                                          >  2:29503/1‑141 (MQ=255)
                                                 gCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAacaa                                                                                        >  1:253748/1‑141 (MQ=255)
                                                    gATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAacaataca                                                                                    >  1:260338/1‑141 (MQ=255)
                                                       tGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTGTTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAacaatacaat                                                                                  <  1:589978/141‑1 (MQ=255)
                                                          aTTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAACTGTTTGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTAt                                                                              >  1:567898/1‑141 (MQ=255)
                                                              gAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCAt                                                                           >  1:567750/1‑141 (MQ=255)
                                                                aTTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACATTACAATGTATCATGc                                                                         <  1:566446/141‑1 (MQ=255)
                                                                aTTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGc                                                                         <  1:27958/141‑1 (MQ=255)
                                                                aTTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGc                                                                         <  1:585238/141‑1 (MQ=255)
                                                                    cGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCtata                                                                     <  2:601031/141‑1 (MQ=255)
                                                                    cGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCtata                                                                     >  1:420823/1‑141 (MQ=255)
                                                                           aaGTTTGCCTTGGATTATTCTAATTGTTATTTTGTTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAATTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTa                                                              <  2:422866/141‑1 (MQ=255)
                                                                              tttGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTaaaa                                                           <  2:191682/141‑1 (MQ=255)
                                                                                   cTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAAtt                                                      >  2:20500/1‑141 (MQ=255)
                                                                                    ttGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAAAAAttg                                                     >  1:96695/1‑140 (MQ=255)
                                                                                           attCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACg                                              >  1:112575/1‑141 (MQ=255)
                                                                                             tCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACgttg                                           >  1:251042/1‑141 (MQ=255)
                                                                                             tCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTAGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATTATGCTATAATGAGTAAAACAATTTGATAAGgtt                                            >  2:308979/1‑141 (MQ=255)
                                                                                             tCTAATTGTTATTTTAGTACATATCTAATTAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTTATAACgtt                                            >  2:162329/1‑141 (MQ=255)
                                                                                                    tttATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGt                                     <  1:117123/140‑1 (MQ=255)
                                                                                                     ttATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGtat                                   >  1:406312/1‑141 (MQ=255)
                                                                                                       aTTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGtata                                  >  2:370803/1‑141 (MQ=255)
                                                                                                        ttttAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATaa                                 >  2:246528/1‑141 (MQ=255)
                                                                                                            aGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAat                            >  1:43042/1‑141 (MQ=255)
                                                                                                             gTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATaaaaaaa                            >  1:525632/1‑140 (MQ=255)
                                                                                                               aCATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAatat                          <  1:196752/141‑1 (MQ=255)
                                                                                                               aCATATCTTGTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAatat                          <  1:196787/141‑1 (MQ=255)
                                                                                                                     cTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGAtt                    >  1:691413/1‑141 (MQ=255)
                                                                                                                       tttCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGAtttac                 >  2:70994/1‑139 (MQ=255)
                                                                                                                          cAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGa               >  1:230860/1‑141 (MQ=255)
                                                                                                                           cattattCCTACATTCGGTTATATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAGCGTTGTTGCGTATAAAAAATTTTAGATTTTCGaaa             <  2:331975/140‑1 (MQ=255)
                                                                                                                           aaTTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGaa              >  1:588000/1‑141 (MQ=255)
                                                                                                                           aaTTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGaaa             <  2:705484/141‑1 (MQ=255)
                                                                                                                           aaTTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGaaa             <  2:705496/141‑1 (MQ=255)
                                                                                                                            aTTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAAt            >  2:301502/1‑141 (MQ=255)
                                                                                                                            aTTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAAt            >  2:485930/1‑141 (MQ=255)
                                                                                                                             ttGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAAt            <  2:112575/141‑1 (MQ=255)
                                                                                                                             ttGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTAGTTGCGTATAAAAAATATTAGATTTTCGAAAt            >  1:506366/1‑141 (MQ=255)
                                                                                                                               gTTCGTACATTCGGTTTCTATTTAAAAAACTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCa          <  1:48904/141‑1 (MQ=255)
                                                                                                                               gTTCGTACATTCGGTTTTAATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCa          <  1:48950/141‑1 (MQ=255)
                                                                                                                                 tCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCATa        <  2:384205/141‑1 (MQ=255)
                                                                                                                                 tCGTACATTCGGTTATATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCATaa       <  1:754232/141‑1 (MQ=255)
                                                                                                                                       aTTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCATAACTATg  <  1:678526/141‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                        
TTTGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCATAACTATG  >  CP000730/1909346‑1909620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: