Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 310,609 A→G 4.8% intergenic (‑54/‑95) USA300HOU_0275 ← / → USA300HOU_0276 GntR family transcriptional regulator/PTS family glucose/glucoside (glc) porter component IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730310,6090AG 4.8% 243.0 / 2.9 85intergenic (‑54/‑95)USA300HOU_0275/USA300HOU_0276GntR family transcriptional regulator/PTS family glucose/glucoside (glc) porter component IIABC
Reads supporting (aligned to +/- strand):  ref base A (41/38);  new base G (2/2);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

ACGCTTGGCAATTTATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGCCCAAGACATTCTTACAG  >  CP000730/310470‑310746
                                                                                                                                           |                                                                                                                                         
aCGCTTGGCAATTTATCACCGGGTTTGAAATTAGATTTATGGATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCGCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAAt                                                                                                                                          <  2:254149/141‑1 (MQ=255)
 cGCTTGGCAATTTATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAAtt                                                                                                                                         >  1:461683/1‑141 (MQ=255)
    ttGGCAATTTATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTa                                                                                                                                      >  1:353216/1‑141 (MQ=255)
      ggCAATTTATCACCGGGTTTAAAATTAGATTGATGTATAAACGCTTTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCAGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAGTTGTATc                                                                                                                                    >  2:416087/1‑141 (MQ=255)
      ggCAATTTATCACCGGGAATGAAATTAACGAGAAGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAGTTGTATc                                                                                                                                    >  2:275746/1‑141 (MQ=255)
        cAATTTATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCgg                                                                                                                                  <  1:653844/141‑1 (MQ=255)
         aaTTTATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGc                                                                                                                                 <  2:763962/141‑1 (MQ=255)
            ttATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGtt                                                                                                                              <  1:75392/141‑1 (MQ=255)
             tATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATCCTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAGTTATTATAGCACAAAATATTATAATTGTATCTGCGgtt                                                                                                                             >  2:636262/1‑141 (MQ=255)
             tATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGttt                                                                                                                             >  1:297237/1‑141 (MQ=255)
              aTCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTa                                                                                                                            <  1:418196/141‑1 (MQ=255)
                 aCCGGGTTTGAAATTAGATTGATGTATAGACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCGCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACaa                                                                                                                         >  1:489066/1‑141 (MQ=255)
                 aCCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACaa                                                                                                                         <  1:614029/141‑1 (MQ=255)
                   cGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAAt                                                                                                                       >  2:283886/1‑141 (MQ=255)
                     gttttGAAATTAGTTTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTg                                                                                                                     <  1:184335/139‑1 (MQ=255)
                         tGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATCGCACAAAATATTATAATTTTATCGGCGTTTATAAATTGTAcc                                                                                                                 >  2:79136/1‑141 (MQ=255)
                          gAAATTAGATTGCTGTGTAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATCGCACAAAATATTATAATTGTATCGGCGTTGAccaggtgaagag                                                                                                                >  2:399093/1‑130 (MQ=255)
                            aaTTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGt                                                                                                              >  2:685861/1‑141 (MQ=255)
                              ttAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTCTAATTGTATCGGCGTTTACAAATTGAACCGGTAc                                                                                                            >  2:479455/1‑141 (MQ=255)
                                aGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACaa                                                                                                          <  1:435753/141‑1 (MQ=255)
                                  aTTGATTTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTGACAACTTGAACCGGTACAAtt                                                                                                        <  1:388254/141‑1 (MQ=255)
                                   ttGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATta                                                                                                       <  2:734114/141‑1 (MQ=255)
                                    tGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACGATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACCATtat                                                                                                      >  2:486616/1‑141 (MQ=255)
                                    tGATGTACAAGCGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACATATTGAACCGGTACAATtat                                                                                                      <  1:403479/141‑1 (MQ=255)
                                               cGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGgta                                                                                           >  1:241869/1‑141 (MQ=255)
                                                   ttATGTTGCTTAGCAATATTTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGgtaagta                                                                                       <  2:84651/141‑1 (MQ=255)
                                                   ttAAGTTGCTTAGCAATATGTTCATACTTTAACCATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGgtaagta                                                                                       <  1:275245/141‑1 (MQ=255)
                                                   ttAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGgtaagta                                                                                       <  1:643399/141‑1 (MQ=255)
                                                   ttAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGgtaagta                                                                                       >  1:473496/1‑141 (MQ=255)
                                                    tAAGTTTCTTACCAATATGTTCATACTTTAACCATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATATCACAAAATACTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGgtaagtaa                                                                                      <  1:362384/141‑1 (MQ=255)
                                                     aaGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTaaa                                                                                     >  2:164913/1‑141 (MQ=255)
                                                      aGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGCGGTAAGTtaaa                                                                                    >  2:23498/1‑140 (MQ=255)
                                                      aGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAAt                                                                                    <  2:168477/141‑1 (MQ=255)
                                                         tGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACa                                                                                 <  1:346324/141‑1 (MQ=255)
                                                           cTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATGCAtt                                                                               >  1:200836/1‑141 (MQ=255)
                                                           cTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACAtt                                                                               >  2:585017/1‑141 (MQ=255)
                                                           cTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGACTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACAtt                                                                               >  2:560756/1‑141 (MQ=255)
                                                            ttAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAGTTGTATCGGCGTTTACTAATTGAACCGGTACAATTCTAATTCGAGGTAAGTAAATACAttt                                                                              <  1:416087/141‑1 (MQ=255)
                                                            ttAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAGTTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACAttt                                                                              <  1:275746/141‑1 (MQ=255)
                                                                cAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCAt                                                                          >  1:288053/1‑141 (MQ=255)
                                                                  atatGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCAtta                                                                        >  1:539566/1‑140 (MQ=255)
                                                                  atatGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTc                                                                        >  1:129227/1‑141 (MQ=255)
                                                                  atatGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAACTGGAAATGAATAAATACATTTTCATTc                                                                        <  2:736765/141‑1 (MQ=255)
                                                                  atatGTTCATACTTGAACAATATTCAGTCCTCGTTTCACTTTGTATGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTc                                                                        >  1:414584/1‑141 (MQ=255)
                                                                   tatGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTct                                                                       <  2:317019/141‑1 (MQ=255)
                                                                     tGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTctac                                                                     >  1:441165/1‑141 (MQ=255)
                                                                         cATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATc                                                                 >  1:202886/1‑141 (MQ=255)
                                                                         cATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGATACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATc                                                                 >  1:160032/1‑141 (MQ=255)
                                                                         cATACTTTAACAATATGCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATc                                                                 >  1:449561/1‑141 (MQ=255)
                                                                           tACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCaa                                                               <  1:75159/141‑1 (MQ=255)
                                                                            aCTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAg                                                              <  1:582000/141‑1 (MQ=255)
                                                                             cTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATCCTTGTATCGGCGTTTACAAATAGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGt                                                             <  2:190401/141‑1 (MQ=255)
                                                                                tAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTATGAAGTGAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGa                                                          >  1:58218/1‑141 (MQ=255)
                                                                                tAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATAAAGTTGa                                                          >  2:744394/1‑141 (MQ=255)
                                                                                    aaTATTCTGTCCTCGTTTCACTTTGTCTGTTATTATTATAGCACATAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTCGGTAAATACATTTTCATTCTACTATCAAGTTGAgggg                                                      <  2:379220/141‑1 (MQ=255)
                                                                                        ttCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTaa                                                  <  1:71404/141‑1 (MQ=255)
                                                                                        ttCTGGCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATGATACTGGTATCTGCGTTTACACATTGAGCCGTTACAATTATAATTGGAGGTAATTAAATACATTTTCATTCTGCTATCAAGTTGAGGGGGTaa                                                  <  2:568846/141‑1 (MQ=255)
                                                                                            gTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGCAATAtt                                              >  2:143529/1‑141 (MQ=255)
                                                                                             tCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATAttt                                             >  1:38960/1‑141 (MQ=255)
                                                                                             tCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATAttt                                             >  1:593681/1‑141 (MQ=255)
                                                                                               cTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTAt                                           <  1:211054/141‑1 (MQ=255)
                                                                                                  gTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACACTTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAg                                        >  2:486630/1‑141 (MQ=255)
                                                                                                  gTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGGGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGAAATCTTTATGAg                                        >  2:397570/1‑141 (MQ=255)
                                                                                                     tgaccttGTCTGTAAGTAATAGAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCaa                                     <  1:531978/136‑1 (MQ=255)
                                                                                                     tCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCaa                                     <  2:102736/141‑1 (MQ=255)
                                                                                                     tCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCaa                                     <  2:244001/141‑1 (MQ=255)
                                                                                                     tCACTTTGTCTGGAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCaa                                     <  2:222981/141‑1 (MQ=255)
                                                                                                      cACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCaat                                    <  1:496856/141‑1 (MQ=255)
                                                                                                       aCTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCaata                                   <  2:353216/141‑1 (MQ=255)
                                                                                                       aCTTTGTCTGTAATTATTACAGCACAAAATATTGAAATCGTATCGGCGGTTACGATTTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATCTTCATTCTACTATCAAGTTGAGGCGGTAATATTTATGAGCaata                                   <  2:415246/141‑1 (MQ=255)
                                                                                                            gTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAatat                              >  1:310035/1‑141 (MQ=255)
                                                                                                              cTGTAATTATTATAGCACAAAATATTATAAGTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATaa                            <  1:670069/141‑1 (MQ=255)
                                                                                                                   attattATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTCCTATCCAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAAc                       <  2:125319/141‑1 (MQ=255)
                                                                                                                   attattATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAAc                       <  1:237867/141‑1 (MQ=255)
                                                                                                                      attatAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAg                    >  1:73255/1‑141 (MQ=255)
                                                                                                                       ttatAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGc                   <  1:415059/141‑1 (MQ=255)
                                                                                                                        tataGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGcc                  >  1:62274/1‑141 (MQ=255)
                                                                                                                        tataGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGcc                  >  1:645288/1‑141 (MQ=255)
                                                                                                                        tataGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTAAAATTGGAGGTAAGTAAATACATTGTCATTCTACTATCAAGTTGAGGGGGTAATATCTATGAGCAATAAATATAAAGAGCAAGcc                  >  2:49681/1‑141 (MQ=255)
                                                                                                                         ataGCACAAAAGATTATAATTGTATCGGCTTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGccc                 <  2:133491/141‑1 (MQ=255)
                                                                                                                          aaGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGCCCa                <  2:574370/140‑1 (MQ=255)
                                                                                                                             cacaAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAAAACAAGCCCAAGa             >  1:37991/1‑141 (MQ=255)
                                                                                                                              acaAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGCCCAAGaa            >  1:340889/1‑140 (MQ=255)
                                                                                                                                     attatAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGCCCAAGACATTCTTa     >  2:145722/1‑141 (MQ=255)
                                                                                                                                       taaaaTTGTATCGGCGTTTACAAATTGAACCGGTACACTTATAATTGGAGGTAAGTAAATACATTTTCATTCGACTATCAAGCTGCAGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGCCCAAGACATTCTTACa   <  1:751684/138‑1 (MQ=255)
                                                                                                                                        ataATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGCCCAAGACATTCTTACAg  <  1:23498/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
ACGCTTGGCAATTTATCACCGGGTTTGAAATTAGATTGATGTATAAACGCATTAAGTTGCTTAGCAATATGTTCATACTTTAACAATATTCTGTCCTCGTTTCACTTTGTCTGTAATTATTATAGCACAAAATATTATAATTGTATCGGCGTTTACAAATTGAACCGGTACAATTATAATTGGAGGTAAGTAAATACATTTTCATTCTACTATCAAGTTGAGGGGGTAATATTTATGAGCAATAAATATAAAGAACAAGCCCAAGACATTCTTACAG  >  CP000730/310470‑310746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: