Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 629,681 C→A 100% L84I (CTA→ATA)  USA300HOU_0564 → possible 3‑hexulose‑6‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730629,6810CA100.0% 188.3 / ‑6.5 57L84I (CTA→ATA) USA300HOU_0564possible 3‑hexulose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (25/31);  minor base G (0/1);  total (25/32)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAT  >  CP000730/629544‑629818
                                                                                                                                         |                                                                                                                                         
aCGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGGTTATGAATTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAg                                                                                                                                        <  1:439870/141‑1 (MQ=255)
  gAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGgt                                                                                                                                      <  1:283560/141‑1 (MQ=255)
    aGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGgtgt                                                                                                                                    <  1:486699/141‑1 (MQ=255)
    aGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGgtgt                                                                                                                                    <  1:607120/141‑1 (MQ=255)
    aGGTTTACCAGCAGTAAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGgtgt                                                                                                                                    <  1:160883/141‑1 (MQ=255)
        ttACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCa                                                                                                                                >  1:189197/1‑141 (MQ=255)
                       cATATGGAAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAAtt                                                                                                                 <  2:242100/141‑1 (MQ=255)
                          atGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTaaa                                                                                                              <  2:91849/141‑1 (MQ=255)
                           tGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAag                                                                                                             <  2:171416/141‑1 (MQ=255)
                                gACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCt                                                                                                        >  1:214490/1‑141 (MQ=255)
                                   aacaTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTAtt                                                                                                     <  2:90915/141‑1 (MQ=255)
                                     caTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTga                                                                                                   >  1:144606/1‑141 (MQ=255)
                                      aTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTgaa                                                                                                  >  1:695912/1‑141 (MQ=255)
                                      aTTAGTAATGTAAAAGTATTAGCAGACATGAAAATCATAGTTGTAGCTGATGATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTgaa                                                                                                  <  1:393621/141‑1 (MQ=255)
                                            aaTGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCt                                                                                            >  2:551293/1‑141 (MQ=255)
                                            aaTGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCGGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCt                                                                                            <  1:709822/141‑1 (MQ=255)
                                              tGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCa                                                                                          >  2:97331/1‑141 (MQ=255)
                                                  aaaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaa                                                                                      <  1:255591/141‑1 (MQ=255)
                                                   aaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaa                                                                                     <  2:312145/141‑1 (MQ=255)
                                                    aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  2:291964/1‑141 (MQ=255)
                                                            cAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTAGCGCGGATATAATTACAATAATAGGTGTTGCAGAAAATGCATCAATAAAAGCAGCAATTGAAGAAGCACAAAAAAATAATaaaa                                                                            >  2:206580/1‑140 (MQ=255)
                                                             aGAAATGAAATTTTTATATGCAGCTGATTATGAATTTAGCTAATCATTTCTATTTTGCGCATATTTAATTACAATAATATTTGTTGCACAATATGCATCAATTAAAGCTTCTATTGAAGAAGCTCATAAAAATAATAAACa                                                                           <  2:276686/141‑1 (MQ=255)
                                                                cATGAAAATTATAGGTGCAGCTGATTATGAAGTTAGCCCAGAAATTGCTTTTCGAGCGGAGCTAATTACAATAATAGGTGTTGCAGAAGAGGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAAtt                                                                        <  2:731426/141‑1 (MQ=255)
                                                                 attaaaaTTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGAGGCATCAATTAAAGCAGCTATTGAAGAAGATCATAAAAATAATAAACAATTa                                                                       >  2:502971/4‑141 (MQ=255)
                                                                 aTGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTa                                                                       >  1:618999/1‑141 (MQ=255)
                                                                  tGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTAc                                                                      <  2:269914/141‑1 (MQ=255)
                                                                   gAAAATTATGGACGCAGCGGATTATGAAGTTAGCCAAGCAATGAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACt                                                                     <  2:303431/141‑1 (MQ=255)
                                                                    aaaaTTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTa                                                                    >  2:600528/1‑141 (MQ=255)
                                                                     aaaTTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAg                                                                   <  2:569675/141‑1 (MQ=255)
                                                                        ttATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAACTAAAAAACAATTACTAGTTg                                                                >  2:629455/1‑141 (MQ=255)
                                                                           taGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTCCTTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGata                                                             >  1:715126/1‑141 (MQ=255)
                                                                            aGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGatgt                                                            >  2:260007/1‑139 (MQ=255)
                                                                            aGATGCAGCTAATTATGAAGGTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGatat                                                            <  1:609464/141‑1 (MQ=255)
                                                                                 cAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCATTTAAAGCAGCTATTGAAGAAGCTCATAAAAATAAAGAACAATTACAAGTCGAAAGGAttt                                                       >  2:456041/1‑140 (MQ=255)
                                                                                 cAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTg                                                       >  2:711943/1‑141 (MQ=255)
                                                                                  aGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTTAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGc                                                      <  1:611468/141‑1 (MQ=255)
                                                                                    cTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTg                                                    <  1:350050/141‑1 (MQ=255)
                                                                                     tGATTATGAAGTTAGACAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGt                                                   <  1:184617/141‑1 (MQ=255)
                                                                                          aTGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATGATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAg                                              >  1:147577/1‑141 (MQ=255)
                                                                                              aGTTATCTAAGCAATTACATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCCATTGAAGAAGCTCATAAATATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGAttt                                          <  2:592022/141‑1 (MQ=255)
                                                                                              aGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGAttt                                          >  1:523242/1‑141 (MQ=255)
                                                                                               tttAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACACTCATGGGAGTTGCAGAGGATGCATCAAATAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTa                                         <  2:440634/140‑1 (MQ=255)
                                                                                               gTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTa                                         <  2:666379/141‑1 (MQ=255)
                                                                                                 tAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGa                                       >  2:297764/1‑141 (MQ=255)
                                                                                                   gCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGaaa                                     >  1:94111/1‑141 (MQ=255)
                                                                                                      aaGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAAc                                  >  1:202658/1‑141 (MQ=255)
                                                                                                        gCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGt                                <  1:291964/141‑1 (MQ=255)
                                                                                                        gCAATTAAATTTGGCGCGGATGAAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGt                                >  2:555278/1‑141 (MQ=255)
                                                                                                            ttAAATTTGGCGCGGATGTAATTAAAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTAATAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCaa                            <  1:39470/141‑1 (MQ=255)
                                                                                                             tAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCATTTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACCTGCaaa                           <  2:476330/141‑1 (MQ=255)
                                                                                                               aaTTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAg                         <  1:228615/141‑1 (MQ=255)
                                                                                                                    ggCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTa                    >  1:33533/1‑141 (MQ=255)
                                                                                                                     gcgcGGATGTAATTACAATAATAGGTGTTGAAGAAGATGCATCAATTAAAGCAGGTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTaa                   >  2:536483/1‑140 (MQ=255)
                                                                                                                       gcgGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGAt                 <  1:551293/141‑1 (MQ=255)
                                                                                                                           aTGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGaaa             >  2:502076/1‑141 (MQ=255)
                                                                                                                                  tACAATAGTGGGTGTTGCAGAATAGTTATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGc      <  2:189197/141‑1 (MQ=255)
                                                                                                                                  gaCAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACGAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGc      <  1:642902/140‑1 (MQ=255)
                                                                                                                                      ataataGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAt  <  2:618999/141‑1 (MQ=255)
                                                                                                                                      ataataGGTGTAGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAt  <  1:107960/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
ACGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAT  >  CP000730/629544‑629818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: