Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9100AG99.4% 236.8 / ‑6.6 72intergenic (‑1/‑121)USA300HOU_0653/USA300HOU_0654ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (30/39);  minor base C (2/0);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAA  >  CP000730/707780‑708046
                                                                                                                                   |                                                                                                                                         
gggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAcc                                                                                                                                 >  2:292915/1‑141 (MQ=255)
  gAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTACCAAGCGTTTATAcctc                                                                                                                               >  1:260514/1‑141 (MQ=255)
  gAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAcctc                                                                                                                               <  2:478293/141‑1 (MQ=255)
         tACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTa                                                                                                                        >  2:278986/1‑141 (MQ=255)
          aCCAGCACCCTTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTACCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaa                                                                                                                       <  2:496278/141‑1 (MQ=255)
          aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGACATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAAGAAGCGTTTATACCTCCTAATTaa                                                                                                                       <  2:543745/141‑1 (MQ=255)
          aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaa                                                                                                                       <  1:627168/141‑1 (MQ=255)
          aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTGCTAACAAGCGTTTATACCTCCTAATTaa                                                                                                                       >  1:431999/1‑141 (MQ=255)
          aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGAACTGGTATCGAGAAGGAAAGGTTTTTCAGTACATGCTTATTCACTAAAAAGAGATATAAATCTTTTGTTACTACCAAGCGTTTATACCTCATAATTaa                                                                                                                       >  2:750337/1‑141 (MQ=255)
           ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTGTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAACCGCTTATAGCTCCTCATTaaa                                                                                                                      >  2:117381/1‑141 (MQ=255)
               cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTGCGGGTATAGATAAGGAAATGCTTTTAAGTAGATGCTTATAACATAAAAACAGAGTTAAATCTTTTTTTTCTAACAAGCGTTTGTACCACCTATGTAATAGtt                                                                                                                  >  2:572568/1‑141 (MQ=255)
               cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGtt                                                                                                                  <  1:678912/141‑1 (MQ=255)
                aCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGttt                                                                                                                 <  1:197539/141‑1 (MQ=255)
                  cATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAg                                                                                                               >  2:36440/1‑141 (MQ=255)
                    ttCGGGTCCATGATACCAATTATTTCGGCGCGTACTGGCCTCGATAAGGAGATGTGTTTAACAACATTTTTAGTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAAGTAAAAGTTTAGGc                                                                                                             <  2:270486/141‑1 (MQ=255)
                       gggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTaa                                                                                                          >  2:2427/1‑141 (MQ=255)
                       gggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTaa                                                                                                          >  1:30567/1‑141 (MQ=255)
                         gaccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGAAAATAAAATAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                        <  2:392375/139‑1 (MQ=255)
                         gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTGTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                        <  1:592510/141‑1 (MQ=255)
                           ccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCta                                                                                                      <  2:171336/141‑1 (MQ=255)
                           ccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATATTCCGATCTGTGCAGGCGTTTCTACCTCCCAATTAAAAGTTTCGGCTAACCta                                                                                                      >  1:357240/1‑141 (MQ=255)
                            cctGATATCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                     <  1:706575/139‑1 (MQ=255)
                            cATGATACCACTTATTTCGCCGCGTACTGGTATCGATAATGAAATGTTTTTAAGTACATGCTTATGACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                     <  2:164076/141‑1 (MQ=255)
                            cATGATACCAATTATTTCGCCGCGTACTGGTATTGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                     <  1:186939/141‑1 (MQ=255)
                            cATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                     <  1:239258/141‑1 (MQ=255)
                            cATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTGTATACCTCCTAATTAAAAGGTTAGGCGAACCtaa                                                                                                     >  1:312724/1‑141 (MQ=255)
                                  aCCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCCaattaatt                                                                                               >  1:342930/1‑141 (MQ=255)
                                   ccAATTATTTCGCGGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTg                                                                                              <  2:61059/141‑1 (MQ=255)
                                   ccAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTACTGACTGTCCGTTTTTTTCTACTACTTAGAACATTAGGCTAACCTAATTAATTg                                                                                              >  1:549193/1‑141 (MQ=255)
                                          tttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataata                                                                                       <  1:535647/141‑1 (MQ=255)
                                            tCGCCGCGTACTGGTATCGAGAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAGCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCCAACCTAATTAATTGTATAATaaa                                                                                     >  1:479262/1‑141 (MQ=255)
                                                 gcgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAGTCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAGTTAATTCTATAATAAACTgag                                                                                <  2:520951/141‑1 (MQ=255)
                                                 aagTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAGAAACAGATTTAAGTCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAGTTAATTATATAATAAACTgag                                                                                <  2:521133/139‑1 (MQ=255)
                                                  ggTACTGCTATCGATCAGGAATTGTTTTTATGTACATGCTTATTACCTAAGGACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAACTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATCATAAACTgaga                                                                               <  2:627563/140‑1 (MQ=255)
                                                       ttatATCGATTAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAATCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAAGAAACTGAGAATAtt                                                                          <  2:251106/138‑1 (MQ=255)
                                                         gTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTa                                                                        <  2:50190/141‑1 (MQ=255)
                                                                     aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                            <  2:121863/141‑1 (MQ=255)
                                                                      aaTGTTTTTAATTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTTCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaa                                                           <  2:645682/141‑1 (MQ=255)
                                                                       aTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                          >  2:642752/1‑141 (MQ=255)
                                                                        tGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                         >  1:639141/1‑141 (MQ=255)
                                                                        agTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTCAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                         <  1:634348/140‑1 (MQ=255)
                                                                             ttAAGTACTTGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                                    >  2:464676/1‑141 (MQ=255)
                                                                               aactACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGat                                                  <  2:564834/138‑1 (MQ=255)
                                                                                 gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatat                                                <  1:495667/141‑1 (MQ=255)
                                                                                 gTACATGCTCTATACCTAAAAACAGATTTACATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatat                                                <  2:481710/141‑1 (MQ=255)
                                                                                  tACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTACACACCCTAATTAAAAGTTTATGCCAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAATTAAATTCTTGatata                                               >  2:360236/1‑141 (MQ=255)
                                                                                     aTGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataaaa                                            >  2:583786/1‑139 (MQ=255)
                                                                                            ttaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa                                    >  1:498862/1‑141 (MQ=255)
                                                                                            ttaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa                                    <  1:330034/141‑1 (MQ=255)
                                                                                            ttaCCAAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa                                    <  2:480442/141‑1 (MQ=255)
                                                                                             taCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATTTTTATCATGTCAAGTAAATTCGTGATATAATATAGaaaaa                                   >  2:386204/1‑140 (MQ=255)
                                                                                              aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                  <  1:215382/141‑1 (MQ=255)
                                                                                              aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTACACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                  <  1:215383/141‑1 (MQ=255)
                                                                                                cTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAGGTATATTCGTGATATAATATAGACAatgta                                <  2:698767/141‑1 (MQ=255)
                                                                                                   aaaaCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatgt                             <  2:641935/141‑1 (MQ=255)
                                                                                                   aaaaCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTAGACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATCTAATATAGACAatgtatgt                             <  1:494209/141‑1 (MQ=255)
                                                                                                         gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTg                       >  1:643449/1‑141 (MQ=255)
                                                                                                         gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTg                       <  1:278986/141‑1 (MQ=255)
                                                                                                         gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTAAGGTg                       >  2:434530/1‑141 (MQ=255)
                                                                                                         gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATAAAAAGTTTAGGCCAACCTAATTAATTGTATAATAAAATGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGGGAGGTg                       >  2:194285/1‑141 (MQ=255)
                                                                                                          aTTTAAATGTTTTGTTTCTAACAAGCGTTTATTGCTCCGATGTTAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGGTATAATATAGACAATGTATGTGAGGTGa                      <  2:342930/141‑1 (MQ=255)
                                                                                                           tttAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGCATATTTATCTTGTCAAGTAAATTCGTGATATAATATACACAATGTATGGGAGGTGaa                     >  1:163086/1‑141 (MQ=255)
                                                                                                            ttAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTGATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCGTGTCAAGTAAATTCGTGATATAATATGAGAAATGTGAGTGAGGCGaaa                    >  1:238701/1‑141 (MQ=255)
                                                                                                            ttAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGaaa                    >  1:418216/1‑141 (MQ=255)
                                                                                                                 tCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATg               <  1:652509/141‑1 (MQ=255)
                                                                                                                    tttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTa            <  1:692469/141‑1 (MQ=255)
                                                                                                                    tttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTa            <  2:431999/141‑1 (MQ=255)
                                                                                                                     ttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAGTTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTCTGTTaa           >  1:663553/1‑141 (MQ=255)
                                                                                                                     ttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTaa           >  1:299895/1‑141 (MQ=255)
                                                                                                                      tGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAAc          <  1:151952/141‑1 (MQ=255)
                                                                                                                            tAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATCAATTCCATCCTAAACTGAGAATATTTATCATGTCAAGAAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaaga    >  2:308004/1‑141 (MQ=255)
                                                                                                                              aCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGaaa  >  1:229748/1‑141 (MQ=255)
                                                                                                                                   |                                                                                                                                         
GGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAA  >  CP000730/707780‑708046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: