Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,243,307 A→T 5.0% S249C (AGT→TGT)  USA300HOU_1165 → fatty acid/phospholipid synthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,243,3070AT 5.0% 230.4 / 2.6 79S249C (AGT→TGT) USA300HOU_1165fatty acid/phospholipid synthesis protein
Reads supporting (aligned to +/- strand):  ref base A (40/35);  new base T (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

TGGGAATATTGAAGCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGCACACGGTAGTTC  >  CP000730/1243168‑1243446
                                                                                                                                           |                                                                                                                                           
tgGGAATATTGAAGCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGAGGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCTGTAAAATGTTAAAAGATACGATTATGag                                                                                                                                            <  1:208368/141‑1 (MQ=255)
 gggAATATTGAAGTGAAGACATTAATTGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGCTCCTTAAAAATTTAGAAGGTACCGCATAATCAATCGGTAAAATGTTAAAAGATACGATTCTGcgt                                                                                                                                           <  2:383106/141‑1 (MQ=255)
       tatGAAGCTAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACt                                                                                                                                     <  2:306304/139‑1 (MQ=255)
       atTGAAGCGAAGACATTAATGGAGGGCGAAACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACt                                                                                                                                     <  2:239949/141‑1 (MQ=255)
        ttGAAGCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGTGTAGTACTa                                                                                                                                    >  2:115534/1‑141 (MQ=255)
        ttGAAGCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGTGTAGTACTa                                                                                                                                    >  2:115460/1‑141 (MQ=255)
          gAAGCGAAGACATTAATGGATGGCGATCCAGATGTTGTAGTTACCGATGGCTTAACAGGAAACAGGGTGCTTAAAAATATAGAATGAACTGCAAAATCAATCGGTAAAAGGTTAAAAGATACGATAATGAGTAGAACTaaa                                                                                                                                  >  2:100881/1‑141 (MQ=255)
          gAAGCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTaaa                                                                                                                                  >  2:607623/1‑141 (MQ=255)
          gAAGCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAATAGTACTaaa                                                                                                                                  >  2:118925/1‑141 (MQ=255)
             gCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTCCTaaaaaa                                                                                                                               >  2:528013/1‑140 (MQ=255)
               gAAAACATAAATGGATGGGGATACAGATGTTGTAGTTACCGAGGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAaaataa                                                                                                                             <  2:460572/141‑1 (MQ=255)
                   aCATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTa                                                                                                                         >  2:594839/1‑141 (MQ=255)
                    cATTGATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTAGTGAAAAATCGTGCAGTAAAATGTTAAAAGATTCGATTATGAGTAGCAATAAAAATAAATTAg                                                                                                                        >  1:517764/1‑141 (MQ=255)
                    cATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAg                                                                                                                        >  1:186513/1‑141 (MQ=255)
                    cATTAATGGATGGCGATACAAATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAg                                                                                                                        >  1:460756/1‑141 (MQ=255)
                         atggatggCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGt                                                                                                                   >  1:255894/1‑141 (MQ=255)
                           ggatggCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGc                                                                                                                 <  2:512373/141‑1 (MQ=255)
                           ggatggCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGc                                                                                                                 <  2:759454/141‑1 (MQ=255)
                           gcatggCAATACCGATCTTGTGGTCACCGGTGGCTAGAAGGGGAACAAGGTCCTCAAAAATTTAGAAGGTAATGCAAAATCAACATGCAAAATGTTAAAAAATACCATCATGAGTAGTACTAAAAATAAATTAGCATGTGc                                                                                                                 <  2:213714/139‑1 (MQ=255)
                                gCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAatat                                                                                                            <  1:108939/141‑1 (MQ=255)
                                   aTACAGATGGTGGAGTTACCGATGGATATACTGGGAACATGGTCCTTAAAAATTTAGAAAGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTga                                                                                                         <  2:724858/141‑1 (MQ=255)
                                     aCAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTtaag                                                                                                       <  2:255894/141‑1 (MQ=255)
                                              gtAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTa                                                                                              >  2:76591/1‑141 (MQ=255)
                                               tatctACCCATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGCACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAg                                                                                             <  1:12544/137‑1 (MQ=255)
                                                aGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATCTTGAAGAAATATTTAGc                                                                                            >  1:126915/1‑141 (MQ=255)
                                                 gTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCt                                                                                           >  2:665035/1‑141 (MQ=255)
                                                   tACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGTAGAAAGATTTAGCTGa                                                                                         <  1:377791/141‑1 (MQ=255)
                                                   tACCGATGGATATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTATTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGGAGTACTAAAAATAAATTAGCAGGTGCAATAATAAAGAAAGATTTAGCTGa                                                                                         >  2:487222/1‑141 (MQ=255)
                                                      cGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAAtt                                                                                      <  2:6472/141‑1 (MQ=255)
                                                       gATGGCGATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTc                                                                                     <  2:470357/141‑1 (MQ=255)
                                                         tGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCACAAGCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGc                                                                                   <  2:363395/141‑1 (MQ=255)
                                                            ttaaaCTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTaa                                                                                <  2:628699/137‑1 (MQ=255)
                                                             tataCTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTaaa                                                                               <  1:463517/141‑1 (MQ=255)
                                                               taCTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTaaaaa                                                                             >  1:105343/1‑141 (MQ=255)
                                                                     gAACATGGTCCTTAAAAATTTAGAAGGTCAAACAAAAGCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGCTGGa                                                                       >  1:366052/1‑141 (MQ=255)
                                                                     gAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGa                                                                       <  2:302710/141‑1 (MQ=255)
                                                                      aaCATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAACTGTAAAATGATACGATCATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGAt                                                                      >  1:177657/1‑141 (MQ=255)
                                                                        cATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTa                                                                    >  2:585015/1‑141 (MQ=255)
                                                                           ggTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAACGATTTAGCTGAATTCGCTAAAAAGATGGATTACTc                                                                 >  2:571305/1‑141 (MQ=255)
                                                                           ggTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTc                                                                 >  1:601953/1‑141 (MQ=255)
                                                                           ggTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTc                                                                 >  2:537483/1‑141 (MQ=255)
                                                                           ggTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTc                                                                 >  1:703416/1‑141 (MQ=255)
                                                                            gTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCa                                                                <  1:764227/141‑1 (MQ=255)
                                                                             tCCTTAACAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATGAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAg                                                               <  1:674651/141‑1 (MQ=255)
                                                                             tCCTTAAAAATTTAGTAGGTACAGCAAAGTCAATCGGCAAAATGTTACAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAg                                                               <  2:218920/141‑1 (MQ=255)
                                                                              ccTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTGGCTAAGAAGAGGGATTACACAGa                                                              >  2:472915/1‑141 (MQ=255)
                                                                              ccTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGa                                                              >  2:192229/1‑141 (MQ=255)
                                                                                  aaaaaTTTAGAAGGTACTGCAAAATCAATCGGTAAACTGGTAAAAGCTACGATTATGAGTAGTACTAAAAATAAATTAGCAGTTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATAc                                                          >  1:479868/1‑141 (MQ=255)
                                                                                     aaTTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACggg                                                       >  2:40819/1‑140 (MQ=255)
                                                                                       tttAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACggtgg                                                     >  2:254434/1‑141 (MQ=255)
                                                                                       tttAGAAGGAACAGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACggtgg                                                     <  2:8844/141‑1 (MQ=255)
                                                                                           gAAGGTACTGCAAAATCTATCGGTAAAATGTTAAACGATACGTTTATGAGTAGTACTAATAATAAATTAGCAGGTGCAATTTTGAACAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACggaaccact                                                 <  2:523608/141‑8 (MQ=255)
                                                                                           gAAGGTACTGCAAAAACAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTcc                                                 <  1:653062/141‑1 (MQ=255)
                                                                                             aGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGt                                               >  1:563/1‑141 (MQ=255)
                                                                                             aGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGt                                               >  2:362682/1‑141 (MQ=255)
                                                                                              ggTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGta                                              <  2:460756/141‑1 (MQ=255)
                                                                                              cagacggCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGta                                              <  2:186513/135‑1 (MQ=255)
                                                                                               gTAATGCAAAAACAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGtat                                             <  2:1388/141‑1 (MQ=255)
                                                                                                 aCTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTCGTTCCGtataa                                           >  2:240227/1‑139 (MQ=255)
                                                                                                 aCTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGAAGTACTACAAATAAATTAGCAGGTGCAATATTGAAGAAAAATTTGGCTGCATTCGCTAAAAAGATGGATTACTCAGAATACGGAGGTTCCGtatta                                           >  2:287733/1‑141 (MQ=255)
                                                                                                       aaaTCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGa                                     >  1:371321/1‑141 (MQ=255)
                                                                                                        aatcaatcGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGAt                                    <  2:318177/141‑1 (MQ=255)
                                                                                                        aatcaatcGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAACAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGAt                                    <  2:16314/141‑1 (MQ=255)
                                                                                                        aatcaatcGGTAAAATGTTAAAAGATACGATTATGAGTAGCACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCCTATTATTAGGAt                                    <  1:537483/141‑1 (MQ=255)
                                                                                                         atcaatcGGTAAAATGTTAAAAGATACGATTATGTGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGAtt                                   <  1:115534/141‑1 (MQ=255)
                                                                                                         atcaatcGGTAAAATGTTAAAAGATACGATTATGTGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGAtt                                   <  1:115460/141‑1 (MQ=255)
                                                                                                             atcGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTTCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGaa                               <  1:713867/141‑1 (MQ=255)
                                                                                                             atcGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGGTTGGag                               >  2:60934/1‑140 (MQ=255)
                                                                                                                ggTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGt                            >  2:470105/1‑141 (MQ=255)
                                                                                                                       tGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAg                     >  2:396802/1‑141 (MQ=255)
                                                                                                                        gTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGt                    >  1:152539/1‑141 (MQ=255)
                                                                                                                        gTTAAAAGATACGAATATGAGTCGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATGTAGCAAAAATCGCTAAAAAGATGGTTTACTCAGAAAACGGTGGTTCCGTATTATTCGGATTTGCAGGTACTGTAGt                    >  2:667906/1‑141 (MQ=255)
                                                                                                                         ttAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGtt                   >  2:438356/1‑141 (MQ=255)
                                                                                                                           aagagtgaggATTATGAGTAGAACTAAAAATAAATGAGCAGGTGAAATATCGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTaa                 <  2:517764/132‑1 (MQ=255)
                                                                                                                                 tACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGcaca           <  1:712922/141‑1 (MQ=255)
                                                                                                                                  aCGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGTTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGCacac          >  2:17790/1‑141 (MQ=255)
                                                                                                                                  aCGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGATAAAAAGATGGATTACTCAGAATACGGTGGTTGCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGCacac          >  1:215998/1‑141 (MQ=255)
                                                                                                                                   cGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGCACACg         >  1:736650/1‑141 (MQ=255)
                                                                                                                                    gATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGCACACgg        <  1:665035/141‑1 (MQ=255)
                                                                                                                                        aTGAGTAGTACTAAAAATAAAGTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCCGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGCACACGGTAGt    <  1:106147/141‑1 (MQ=255)
                                                                                                                                          gAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGCACACGGTAGTTc  <  1:426614/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TGGGAATATTGAAGCGAAGACATTAATGGATGGCGATACAGATGTTGTAGTTACCGATGGCTATACTGGGAACATGGTCCTTAAAAATTTAGAAGGTACTGCAAAATCAATCGGTAAAATGTTAAAAGATACGATTATGAGTAGTACTAAAAATAAATTAGCAGGTGCAATATTGAAGAAAGATTTAGCTGAATTCGCTAAAAAGATGGATTACTCAGAATACGGTGGTTCCGTATTATTAGGATTGGAAGGTACTGTAGTTAAAGCACACGGTAGTTC  >  CP000730/1243168‑1243446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: