Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 217.6 / ‑6.5 63I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (30/32);  minor base C (0/1);  total (30/33)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416322‑1416594
                                                                                                                                        |                                                                                                                                        
tttAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTa                                                                                                                                      >  2:397894/1‑141 (MQ=255)
   aaCTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                   <  2:29628/141‑1 (MQ=255)
   aaCTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                   <  1:80298/141‑1 (MQ=255)
     cTCAATCATAGCAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAc                                                                                                                                 <  2:391067/141‑1 (MQ=255)
        aaTCACAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                                                              <  1:539466/141‑1 (MQ=255)
             tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGCTTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                         <  2:378994/141‑1 (MQ=255)
             tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                         <  1:651216/141‑1 (MQ=255)
                     ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATg                                                                                                                 <  2:703029/141‑1 (MQ=255)
                     ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATCGTACTAACAATATTTCAATACaag                                                                                                                 >  2:200327/1‑139 (MQ=255)
                      ctctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGa                                                                                                                >  2:339210/1‑141 (MQ=255)
                         tttcactgtgTTTTATATTTAATTAGACGTTCAGTATATGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATCATTTGACCTTCTTTTAAATCATAATTGTACTGACGATATTTCAATACATGACtt                                                                                                             <  1:492440/135‑1 (MQ=255)
                         tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                             <  2:557364/141‑1 (MQ=255)
                             aTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAGATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                         >  2:336327/1‑141 (MQ=255)
                              ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                        <  1:707785/141‑1 (MQ=255)
                                 tgtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtg                                                                                                     <  2:302739/141‑1 (MQ=255)
                                  gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAACTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTCCAATACATGACTTAAGTTAtgg                                                                                                    >  2:463671/1‑140 (MQ=255)
                                  gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgt                                                                                                    <  2:42367/141‑1 (MQ=255)
                                   ttCTATATTTAATTAGGCGTTAAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTTACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtg                                                                                                   <  2:456471/141‑1 (MQ=255)
                                    tCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgt                                                                                                  <  2:437843/141‑1 (MQ=255)
                                     cTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACATCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAATTAAGTTAtgtgtg                                                                                                 >  2:236607/1‑141 (MQ=255)
                                       atatTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAAAATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACGTGACTTAAGGTAtgtgttaa                                                                                               >  2:591767/1‑138 (MQ=255)
                                              aTTAGACGTTTAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaat                                                                                        <  2:54983/141‑1 (MQ=255)
                                              aTTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGTCTTAAGTTATGTGTGATaataaat                                                                                        <  1:483278/141‑1 (MQ=255)
                                               ttAGACGTTCAGTATACGGATGCAAATGCTTATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaata                                                                                       <  1:762162/141‑1 (MQ=255)
                                               ttAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaata                                                                                       <  1:761903/141‑1 (MQ=255)
                                                tAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAataaataa                                                                                      <  2:441291/141‑1 (MQ=255)
                                                 aGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTAACTCGTTCAATGCTTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACAGGACTTAAGTTATGTGTGATAATAaataat                                                                                     >  1:252527/1‑141 (MQ=255)
                                                   aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGTCAATATTTCAATACATGACTTAGGTTATGTGTGATAGTAAATATTGt                                                                                   >  1:683640/1‑141 (MQ=255)
                                                       tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                               >  1:83177/1‑141 (MQ=255)
                                                        cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                              <  2:737535/141‑1 (MQ=255)
                                                         aGTATACGGATGCGAATGCCCATACTTGAAACGATTAATACTACCGCGTTCAATGATTTGACCTTCTGTTAAAACATAATGGTACAGTCTTTATGTCAAAAAATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                                                                             <  1:107540/141‑1 (MQ=255)
                                                           tataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                           >  2:570741/1‑141 (MQ=255)
                                                              aCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                        <  2:433487/141‑1 (MQ=255)
                                                              aCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                        >  1:604616/1‑141 (MQ=255)
                                                              aCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                        <  1:11591/141‑1 (MQ=255)
                                                                     cAAATGCTCATACTTGAAATGATTAATATTTTCTTGTTCAATGATTTGACCTTCTTTTAAATCATAATTGTACTGACAATATTTCAATACATGACAAAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                                                                 <  2:695408/141‑1 (MQ=255)
                                                                       aaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACaa                                                               >  1:80561/1‑141 (MQ=255)
                                                                          gCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACActat                                                            >  1:429182/1‑141 (MQ=255)
                                                                            tCATACTTGAAATGATTAATATTACCTCCTTCAATAATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATTACTTAATTTATTTGTAATAATAAATAATGTTTGACCATGTTCTAATACAATATGc                                                          <  1:544979/141‑1 (MQ=255)
                                                                              aTACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTg                                                        >  1:190063/1‑141 (MQ=255)
                                                                               tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGt                                                       >  1:737437/1‑141 (MQ=255)
                                                                               tACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGt                                                       >  1:102792/1‑141 (MQ=255)
                                                                                aCTTGAAATGATTAATATTACCTCGTTTAATGATTTGACTTTCTTTTCGACTGTCCTTGGGGGGGCATTTTTTTTAGACATGATTTAAGTTATGAGTCATAATAAATAATGTTTGACTAGGTTCTAATACAATATGCTGta                                                      >  1:751704/1‑141 (MQ=255)
                                                                                     aaaTGATTAATATTGCCTCGTTCAATGATATGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATaaa                                                 >  1:405923/1‑141 (MQ=255)
                                                                                       aTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATc                                               >  1:260230/1‑141 (MQ=255)
                                                                                       aTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGATGTAATAAATc                                               >  2:111247/1‑141 (MQ=255)
                                                                                       aTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAAAATATTTCAATACATGACTTAAGTTAAGTGTGATAATAAAGAAGGTTAGACGGTGTGCTAATACAATATTCGGTAATAAATc                                               >  2:718807/1‑141 (MQ=255)
                                                                                         gATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCa                                             <  1:339210/141‑1 (MQ=255)
                                                                                             aaTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAACTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCAc                                         <  1:45760/141‑1 (MQ=255)
                                                                                              atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACt                                        >  2:745247/1‑141 (MQ=255)
                                                                                              atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACt                                        >  1:15012/1‑141 (MQ=255)
                                                                                              atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACt                                        >  2:685344/1‑141 (MQ=255)
                                                                                              atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACt                                        >  1:678862/1‑141 (MQ=255)
                                                                                                atTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTg                                      <  2:662513/141‑1 (MQ=255)
                                                                                                atTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAAGATGCTGTAATAAATCCATCACTTg                                      >  1:377936/1‑141 (MQ=255)
                                                                                                 ttACCTCGTTGAATGATTTGACCTTCTTTTAAAACATAATTGTACTGATAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGa                                     <  2:247628/141‑1 (MQ=255)
                                                                                                             aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaaa                         >  1:467794/1‑141 (MQ=255)
                                                                                                                  ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAGAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAGCCATCACTTGATTACCGTTCAAAGCATc                    >  1:527496/1‑141 (MQ=255)
                                                                                                                      ccTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAat                <  1:569002/141‑1 (MQ=255)
                                                                                                                       cttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatg               <  2:83177/141‑1 (MQ=255)
                                                                                                                        ttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACGATATGCTGTAATAAATCAATCACTTGATTACCGTTCAAAGAATACAatga              >  2:710588/1‑141 (MQ=255)
                                                                                                                                    aTAATTGTACTGACAATATTTCATTACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  <  2:79418/141‑1 (MQ=255)
                                                                                                                                    aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  <  2:236829/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416322‑1416594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: