Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,646,1890GC100.0% 182.8 / ‑6.5 58A155G (GCA→GGA) USA300HOU_1531M24B family Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (34/22);  minor base T (0/1);  total (35/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACG  >  CP000730/1646051‑1646325
                                                                                                                                          |                                                                                                                                        
atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTACTTCTTTTTCAGTCATGcctcct                                                                                                                                        >  2:442136/1‑141 (MQ=255)
 ttttATCTCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGTTGCTACAGTCGTATCGAATGATGGTCCATCTGCTCCTAGTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                       <  2:338982/141‑1 (MQ=255)
  tttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCttt                                                                                                                                      >  1:119907/1‑141 (MQ=255)
   ttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCATTCATGCCTCCtttt                                                                                                                                     >  2:740577/1‑141 (MQ=255)
     aTCCCTTGCAACACCCTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTCATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                   <  2:102457/141‑1 (MQ=255)
       cACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCTTCCTTTTccaa                                                                                                                                 >  2:317902/1‑141 (MQ=255)
                  ccATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCCTCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAata                                                                                                                      >  2:683320/1‑141 (MQ=255)
                    aTGTGGTAATGCACCTCTATGATCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatata                                                                                                                    <  2:261337/141‑1 (MQ=255)
                    aTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatata                                                                                                                    <  2:95687/141‑1 (MQ=255)
                     tgtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATGTGCTCCTAATTCTAGCATTTTACTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAgtatat                                                                                                                   >  2:553783/1‑141 (MQ=255)
                      gtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCCGTCATGCCTCCTTTTACAACAGTAAAAATATAtt                                                                                                                  >  1:97684/1‑141 (MQ=255)
                       tgGTAATGTGCCTGTAGGGCCAGCTTCTACAATCGTGTCGAATGATGGTCCATTTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTc                                                                                                                 <  1:122915/141‑1 (MQ=255)
                         gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                                                                               <  2:538729/141‑1 (MQ=255)
                          tAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGATCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTANTTTNANAACAGTTAAAATATATTCata                                                                                                              >  1:428308/1‑141 (MQ=255)
                              gCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCTTATGtt                                                                                                          >  1:358189/1‑141 (MQ=255)
                              gCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGtt                                                                                                          >  1:358198/1‑141 (MQ=255)
                                   tctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatc                                                                                                     <  1:274723/141‑1 (MQ=255)
                                   tctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatc                                                                                                     >  1:187542/1‑141 (MQ=255)
                                     tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCTTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                   >  2:185911/1‑141 (MQ=255)
                                     tATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                   >  1:732485/1‑141 (MQ=255)
                                                      aTCGTATCGAATGATGGTCCATCTGCTGCTAATTCTAGCATTTTTCTTTCTAAGATTGCCTTTAATTCTTTTTCAGTCACGCCTCCTTTTACAACAGTTAGAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                  <  1:340846/141‑1 (MQ=255)
                                                      aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                  >  1:372904/1‑141 (MQ=255)
                                                           aTCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                             >  1:77676/1‑141 (MQ=255)
                                                              gAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                          >  1:295147/1‑141 (MQ=255)
                                                              gAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                          <  2:77676/141‑1 (MQ=255)
                                                              gAATGATGGTCCATCTGCTCCTAACTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                          <  1:240755/141‑1 (MQ=255)
                                                              caaTGATGGTTCCTCTGCTCCTGATTCTAGCGTTTTGCTTTCTAATATTGCCTTTTATTCTTTTTCAGTTATGCCTCTTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                                                                          <  2:263925/140‑1 (MQ=255)
                                                                atgatgGTCCATCCGCTCATAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAg                                                                        <  1:472249/141‑1 (MQ=255)
                                                                      gTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAttt                                                                  <  2:167772/141‑1 (MQ=255)
                                                                        ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCg                                                                >  1:330761/1‑141 (MQ=255)
                                                                          aTCTGCTTCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTc                                                              <  2:330140/141‑1 (MQ=255)
                                                                            cTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAg                                                            >  1:463379/1‑141 (MQ=255)
                                                                            cTGCTCCTAATTCTAGCATTATGCTTTCTAATATTGCCTTTAATACTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATGTTAGCTGGTTTTTGAATTGAAGCAATTTCGTCAg                                                            >  1:460135/1‑141 (MQ=255)
                                                                               cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                         >  1:419219/1‑141 (MQ=255)
                                                                               cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCCGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                         >  1:419242/1‑141 (MQ=255)
                                                                                 ccTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTTTTTTTTTTTTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTTAATTAAATCACTTTCGCCACCATCt                                                       >  2:265705/1‑141 (MQ=255)
                                                                                       tCTAGCCTTTTGCTTTCTTACCTTGCGTTTTATTTCTTTTCAGTCTTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCTTCAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACg                                                 <  1:541739/141‑1 (MQ=255)
                                                                                        cTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGt                                                >  1:744000/1‑141 (MQ=255)
                                                                                          aGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                              >  2:263812/1‑141 (MQ=255)
                                                                                           gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAGGCGAGTTCGTCAGCATCTTTGACGtttc                                             >  1:586539/1‑141 (MQ=255)
                                                                                                tcgCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                        <  1:169124/139‑1 (MQ=255)
                                                                                                  gCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtttt                                      >  1:185399/1‑141 (MQ=255)
                                                                                                       cTAATATTGCCTTTAATTCTTTTTCAGTCATGTCCCCTGTTACAACAGTTAAATTATTTTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAACCAATTTCGTCAGCTTATTTGAGTTCTCTAATTTTATCtt                                 >  2:328598/1‑140 (MQ=255)
                                                                                                       cTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTa                                 >  1:346309/1‑141 (MQ=255)
                                                                                                        tAATATTGCCTTTAATTCTTTTTCAGCGAGCCCGCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTAc                                >  1:559569/1‑141 (MQ=255)
                                                                                                              tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTAtt                          <  1:442136/141‑1 (MQ=255)
                                                                                                              tGCCTTTAATTCTTATTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTACGTCAGCCTCTTTGACGTCTCTAATTTTATCTACAGTAtt                          <  1:543720/141‑1 (MQ=255)
                                                                                                                  tttAATTCTTCTTAAGTCATGCCTCCTAATACAACAGTTAAAATATATTCATATGATTCATCAACAATATTCGCGGCTTTTTGATTTAAAACAATGTCGTCAGCATCTTTGACGTCTGTAATTTTATCTACCGTCTTAGaa                      >  2:267816/1‑141 (MQ=255)
                                                                                                                   ttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGATGGCGCTAATTTTATCTACAGTATTAGaaa                     >  2:120690/1‑141 (MQ=255)
                                                                                                                   tcaaTTCTTTTTCAAGTATGCCTCCTTTTACAACAGTTAAAATATATTCCTATGTTTCATCACGCATGTTAGCTGCTCTTTGATTTAAAGCAATTTCGTCATCATCTTTGACGTCTCTAATTTTATCGACAGTATTAGaaa                     <  2:346435/139‑1 (MQ=255)
                                                                                                                     aaTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                   <  1:605051/141‑1 (MQ=255)
                                                                                                                         cTTTTTCCGTCATGCCTTCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta               <  1:520027/141‑1 (MQ=255)
                                                                                                                          tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACCGTATTAGAAATGCttat              >  2:515224/1‑141 (MQ=255)
                                                                                                                           ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatt             <  1:500667/141‑1 (MQ=255)
                                                                                                                                   cATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATAGTAGATGCTTTTTGAATTAAAGCAAGTTAGTCAGCATCTTTGACGTCTCTAATTTTATCTTAAGTATTCGAAATTCTTGTTAataatag     >  2:79982/1‑140 (MQ=255)
                                                                                                                                     tGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc   >  1:629042/1‑141 (MQ=255)
                                                                                                                                     tGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc   >  1:47637/1‑141 (MQ=255)
                                                                                                                                     tGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATAc   <  2:744000/141‑1 (MQ=255)
                                                                                                                                      gCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACg  >  1:397678/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                        
ATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACG  >  CP000730/1646051‑1646325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: