Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,028,269 G→C 100% P126A (CCA→GCA)  USA300HOU_1882 ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,028,2690GC95.6% 217.0 / 1.2 67P126A (CCA→GCA) USA300HOU_1882hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base C (26/38);  total (26/41)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAAC  >  CP000730/2028134‑2028402
                                                                                                                                       |                                                                                                                                     
ccTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATATACTGCTGTAc                                                                                                                                  <  1:551303/141‑1 (MQ=255)
   gCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGtt                                                                                                                               >  2:71853/1‑141 (MQ=255)
    cACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTg                                                                                                                              <  1:387145/141‑1 (MQ=255)
     aCTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGc                                                                                                                             <  2:278177/141‑1 (MQ=255)
     aCTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGc                                                                                                                             >  2:38416/1‑141 (MQ=255)
               aaaTGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAAcc                                                                                                                   <  1:651685/141‑1 (MQ=255)
                aaTGTGTCTAACAACAGTGGTATTATTTTCCTTAATATCTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTAATATACATAAACTGCTGTACGTTGCTCACCAAACCa                                                                                                                  >  1:421657/1‑141 (MQ=255)
                aaTGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCa                                                                                                                  <  2:511250/141‑1 (MQ=255)
                    tgtCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACttt                                                                                                              <  2:139040/141‑1 (MQ=255)
                      tCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGTTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTTCGTTGCTCACCAAACCACTTTTg                                                                                                            <  2:544085/141‑1 (MQ=255)
                          acaacaATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTta                                                                                                        <  2:738289/141‑1 (MQ=255)
                           caacaaTGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAACTAACTATCTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTtaa                                                                                                       <  1:745764/141‑1 (MQ=255)
                                 tGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGACAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAg                                                                                                 <  2:283605/141‑1 (MQ=255)
                                 tGGTATTATTTTCCAGAATTTTTGCCGGTTTTGTTAAGTTAATATGTAGGTCGCCAATTTCATGTTGAATTTGTACATCTTCCAACTTATATACATAAACTGCTGTACGTTGCTATCCAAACTAATTTTGTTTaaagaaag                                                                                                 <  2:107848/141‑1 (MQ=255)
                                  ggTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGACAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGa                                                                                                <  1:64296/141‑1 (MQ=255)
                                   gTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGAt                                                                                               <  1:487661/141‑1 (MQ=255)
                                        attTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGCTGGTGa                                                                                          >  2:187347/1‑141 (MQ=255)
                                            tCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTc                                                                                      >  2:358391/1‑141 (MQ=255)
                                                       tGCAGCTTTTGTTCAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATc                                                                           <  2:548217/141‑1 (MQ=255)
                                                        gCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTGATAACCTCATcc                                                                          >  2:308476/1‑141 (MQ=255)
                                                        gCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATcc                                                                          >  2:762952/1‑141 (MQ=255)
                                                         cAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCGTAACCTCATcct                                                                         >  2:593988/1‑140 (MQ=255)
                                                          aGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGt                                                                        >  1:295999/1‑141 (MQ=255)
                                                           gCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTc                                                                       >  1:57549/1‑141 (MQ=255)
                                                             ttttGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTGTATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAGCCTCAGCcgccgc                                                                     >  1:252947/1‑141 (MQ=255)
                                                               ttGTTAAGTCAATATGTAGGTCCCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCaa                                                                   >  2:555086/1‑141 (MQ=255)
                                                                 gTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAact                                                                 >  2:60406/1‑141 (MQ=255)
                                                                  ttAAGTCAATATGTAGGTCGGCAATTCCATGTTGAATTTGTACACCTTCCCTCTTATATACCTAAACTGCCGTACGTTGCTCCCCAACCCCCTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCCacta                                                                <  2:503309/141‑1 (MQ=255)
                                                                   tAAGTCAATATGTAGGTCGCCAATTCCATGTTGTATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAactac                                                               <  2:424284/141‑1 (MQ=255)
                                                                   tAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAactac                                                               <  2:12224/141‑1 (MQ=255)
                                                                    aaGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAactact                                                              <  1:102019/141‑1 (MQ=255)
                                                                      gTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACttt                                                            >  1:663826/1‑141 (MQ=255)
                                                                      gTCAATATGTAGGTAGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACttt                                                            >  2:516639/1‑141 (MQ=255)
                                                                       tCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACtttt                                                           <  1:760655/141‑1 (MQ=255)
                                                                         aaTATGTAGGGCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACtttttt                                                         <  2:373154/141‑1 (MQ=255)
                                                                           tatGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTg                                                       >  1:306395/1‑141 (MQ=255)
                                                                           tatGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTGTTGTTTAATAAAAGATGGTGAAGCCATAACCTCCTCCGTCGCaacaacgctctctg                                                       >  2:114515/1‑133 (MQ=255)
                                                                              gTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGtt                                                    <  2:273911/141‑1 (MQ=255)
                                                                              gTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGtt                                                    <  2:397344/141‑1 (MQ=255)
                                                                                aGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGCCGCAACTACTTTTTTTGGTTTa                                                  >  1:592021/1‑141 (MQ=255)
                                                                                aGGTCGCCAAGTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTCTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGACGCTACTATTTTTTTTTGTTTa                                                  <  2:7154/141‑1 (MQ=255)
                                                                                       cAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCACCTACTTTTTTTGGTTTAAACTtgt                                           >  1:44472/1‑141 (MQ=255)
                                                                                          ttCCATGTTGAATTTTTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTa                                        <  2:78525/141‑1 (MQ=255)
                                                                                            ccATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTAtt                                      <  2:13547/141‑1 (MQ=255)
                                                                                             cATGTTGAATTTGTACATCTTCCCACTTATATCCATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGTTGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTa                                     <  2:215198/141‑1 (MQ=255)
                                                                                              aTGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAACCCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGAAACTACTTTTTTTGGTTTAAACTTTTTTATTaa                                    >  2:690128/1‑141 (MQ=255)
                                                                                              aTGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTaa                                    >  2:231323/1‑141 (MQ=255)
                                                                                                gTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAAt                                  >  2:337837/1‑141 (MQ=255)
                                                                                                gTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAAt                                  <  1:683820/141‑1 (MQ=255)
                                                                                                     aTTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATTGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGc                             <  1:477123/141‑1 (MQ=255)
                                                                                                     aTTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTACACTTGTGTATTAAATAACGc                             <  2:7315/141‑1 (MQ=255)
                                                                                                       ttGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGcac                           <  1:762952/141‑1 (MQ=255)
                                                                                                          tACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAAt                        >  1:60650/1‑141 (MQ=255)
                                                                                                          tACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAAt                        <  1:49854/141‑1 (MQ=255)
                                                                                                              tctcCCCACTTATATACATAAACTGCTGTACGTCGCTCACCAAACCACTATAGCTAAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTCCTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGt                    <  2:414662/141‑1 (MQ=255)
                                                                                                              tCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGt                    <  1:657581/141‑1 (MQ=255)
                                                                                                               cctCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGta                   <  1:253224/139‑1 (MQ=255)
                                                                                                                ttCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGtaa                  <  1:746785/141‑1 (MQ=255)
                                                                                                                ttCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGtaa                  <  1:358391/141‑1 (MQ=255)
                                                                                                                   ccACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGtaataa               <  2:295999/141‑1 (MQ=255)
                                                                                                                    cACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTTTTTTTTTTTTGTGTATTACATAACGCACAATACGtaatact              >  1:27370/1‑139 (MQ=255)
                                                                                                                           ataCATGATCTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGa       <  2:453422/141‑1 (MQ=255)
                                                                                                                           ataCAGAAACTGCTGGACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACTTCATCCGTCGCAACTTCTTTTTTTGGTTTAAACTTGTGTCTTCGGTCTCTCACAATAAGTAATAATAAAAAGa       <  1:181200/141‑1 (MQ=255)
                                                                                                                            taCATAAACTGCTGTACGTTGCTCACCAAACCACTTATGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATACGAAATAATAAAAAGAc      <  1:421050/141‑1 (MQ=255)
                                                                                                                             aCATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTGGGTTTAAACTTGTGTATTAAATAACGCCCAATAATTAATAATAAAAACACg     >  2:668377/1‑141 (MQ=255)
                                                                                                                              cATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGa    >  1:742602/1‑141 (MQ=255)
                                                                                                                                tacaCTGCTGTACGATGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAAc  <  1:71853/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACGAAC  >  CP000730/2028134‑2028402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑