Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,211,990 C→T 5.9% V196I (GTC→ATC)  USA300HOU_2087 ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,211,9900CT 5.9% 193.8 / 3.2 68V196I (GTC→ATC) USA300HOU_2087hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (29/34);  new base T (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

TTTTTGCCTTTTTTATTGCTGCCGCCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGCTTGGATAAAGT  >  CP000730/2211854‑2212130
                                                                                                                                        |                                                                                                                                            
tttttGCCTTTTTTATTGCTGCCACCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACtgactgac                                                                                                                                          <  1:299184/141‑1 (MQ=255)
ttttgGCCTTTTTTATTGCTGCCGCCGTTGTGCTCTCTTTCATACGCTTCCATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACtgactgac                                                                                                                                          <  1:60898/141‑1 (MQ=255)
  tttGCCTTTTTTATTGCTGCCGCCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCa                                                                                                                                        >  1:504852/1‑141 (MQ=255)
  tttGCCTTTTTTATTGCTGCCGCCATTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCa                                                                                                                                        >  1:485705/1‑141 (MQ=255)
      gcttgtctcgtgctgcCGCCGTTGTGGCCGCTTCCATTCTCGTCAATGAAAGGTTGTACTTCTTGTTGAGCGACTGTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTAc                                                                                                                                    <  1:532580/131‑1 (MQ=255)
        ttttttATTGCTGCCGCCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGTAACGCCGCACTGACTGACCAGTACaa                                                                                                                                  >  2:439077/1‑141 (MQ=255)
         tttttATTGCTGCCGCCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCGGCACTGAATAACCAGTACaaa                                                                                                                                 >  2:214290/1‑141 (MQ=255)
                 gctgcCGCCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGtgc                                                                                                                         >  1:686978/1‑141 (MQ=255)
                  ttttcgccgTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAAACAAGtgct                                                                                                                        >  2:199466/5‑141 (MQ=255)
                   tgcCGCCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGtgctg                                                                                                                       <  1:244362/141‑1 (MQ=255)
                   tgcCGCCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTAATTCTTTTTTAGCGACTTTTTCATGATAAATGTTCGCACAGTGTGTTTGGCCTAAACGGACGGACGCATTGACTGACCAATACAAAcgatgttgtg                                                                                                                       >  2:522122/1‑132 (MQ=255)
                     ccgccgTTGTGCTCTCTTACATAAGCTTAAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGtgctgat                                                                                                                     <  2:369770/141‑1 (MQ=255)
                       gccgTTGTGCTCTCTTTCATACGCTTCAATGAAGGGGTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGtgctgatgc                                                                                                                   >  1:754775/1‑141 (MQ=255)
                        ccgTTGTGCTCTCCTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTAAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGtgctgatgct                                                                                                                  <  2:733186/141‑1 (MQ=255)
                         cgTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTCCGTCTTTGGTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACGAACCAAGtgctgatgctg                                                                                                                 <  1:711394/141‑1 (MQ=255)
                          gTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGtgctgatgctga                                                                                                                >  1:381404/1‑141 (MQ=255)
                          gTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGtgctgatgctga                                                                                                                >  2:722875/1‑141 (MQ=255)
                           ttGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGATTGACCAGTACAAACCAAGtgctgatgctgaa                                                                                                               >  2:372613/1‑140 (MQ=255)
                           ttGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGATTGACCAGTACAAACCAAGTGCTGATGCTGAg                                                                                                               >  2:372532/1‑141 (MQ=255)
                           ttGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTATAAACCAAGTGCTGATGCTGAg                                                                                                               <  1:394669/141‑1 (MQ=255)
                           ttGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAg                                                                                                               >  2:740126/1‑141 (MQ=255)
                               gatctctTTCATACTTTTCAATGAAAGGTTGTACTTCTTGTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGATCTACAAGCAATGCCGCACTGACTGACCAGTACAACCCAAGTGCTGATGCTGAGCTTa                                                                                                           <  2:227718/139‑1 (MQ=255)
                                 tctctTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAAt                                                                                                         >  2:246261/1‑141 (MQ=255)
                                     cttCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACTAGTACAAGCAAAATGCTGACGCGTAGCTTAATGaaa                                                                                                     >  2:120892/2‑141 (MQ=255)
                                          tACGCTTTAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAg                                                                                                <  2:191610/141‑1 (MQ=255)
                                            cGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACACCGCCCTGACTGACGATGACAAAGCACGTGCTGATGCCGAGCTTACTGAAATCCAGAt                                                                                              >  2:691713/1‑141 (MQ=255)
                                              cTTCAATTAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATTTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATaa                                                                                            <  1:515073/141‑1 (MQ=255)
                                                tCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATc                                                                                          <  2:33033/141‑1 (MQ=255)
                                                  aaTGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCAt                                                                                        <  2:597578/141‑1 (MQ=255)
                                                     gAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGACAATCATAAt                                                                                     >  2:249603/1‑141 (MQ=255)
                                                        aGGTTGTACTTCTTTTTTTTTTTTTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTAATGCTGAGCTTAATGAAATCCAGTTAATCTTAATTgt                                                                                  >  2:608964/1‑140 (MQ=255)
                                                        aGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTgg                                                                                  >  2:475261/1‑141 (MQ=255)
                                                        aGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTgg                                                                                  <  2:743484/141‑1 (MQ=255)
                                                        aGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAAACATAATTgg                                                                                  >  2:727639/1‑141 (MQ=255)
                                                            tGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGaa                                                                              >  2:533515/1‑141 (MQ=255)
                                                               aCTTTTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATg                                                                           <  1:266320/141‑1 (MQ=255)
                                                                            cGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACcatcatcatct                                                              >  2:140419/1‑139 (MQ=255)
                                                                            cGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGt                                                              >  2:472410/1‑141 (MQ=255)
                                                                                 ttttCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAAccc                                                         >  1:684140/1‑141 (MQ=255)
                                                                                   ttCATAATAAATGTTCGCAAAGTGTGTTTGAAATACAAGGAACGCCGCACTGACTGACCAGTCCAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCAt                                                       >  1:545383/1‑141 (MQ=255)
                                                                                      ataataAATGTTCGCAAAGTGTTTTTGAACTACAAGGACCGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTg                                                    <  2:291142/141‑1 (MQ=255)
                                                                                      ataataAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTg                                                    <  1:246261/141‑1 (MQ=255)
                                                                                       taataaATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAACCCGAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGa                                                   >  1:104089/1‑141 (MQ=255)
                                                                                         ataaATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACg                                                 <  2:686978/141‑1 (MQ=255)
                                                                                          taaATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACgt                                                <  1:726125/141‑1 (MQ=255)
                                                                                           aaaTGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACgtt                                               <  1:501354/141‑1 (MQ=255)
                                                                                            aaTGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACgttg                                              <  2:398299/141‑1 (MQ=255)
                                                                                              tGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACgttgtt                                            >  2:62630/1‑141 (MQ=255)
                                                                                              tGTTCGCAAAGTGTGTTTGAACTAAAAGGAACGCCGCACTGACTGACCAGTACAAAAAAAGTGTTGATGCTGACCTTAATGAAATCTAGATAATACTAATTGGTGAAATGACCCTACTCATGTACACCAATTGACgtagtt                                            >  2:105941/1‑141 (MQ=255)
                                                                                               gTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTTCCCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTc                                           <  1:129634/141‑1 (MQ=255)
                                                                                               gTTCGCAAAGTGTGTTTGAACAACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATCATCCCAACTGGTGAAATGACCATCATCATGTACCCCATTTGACGTTGTTc                                           >  1:393402/1‑141 (MQ=255)
                                                                                                ttCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCg                                          >  1:479816/1‑141 (MQ=255)
                                                                                                 tCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAGCCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATATTCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGt                                         <  2:648410/141‑1 (MQ=255)
                                                                                                 tCGCAAAGTGTGGTTGAACTACAAGGAACGGCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGt                                         <  2:648525/141‑1 (MQ=255)
                                                                                                    cAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTg                                      <  1:223563/141‑1 (MQ=255)
                                                                                                    cAAAGGGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTg                                      <  1:17692/141‑1 (MQ=255)
                                                                                                       aGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCa                                   <  2:381404/141‑1 (MQ=255)
                                                                                                       aGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCa                                   <  1:380293/141‑1 (MQ=255)
                                                                                                         ggtgtTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATACTCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATc                                 <  2:754775/140‑1 (MQ=255)
                                                                                                             tttGAACTACAAGGAACGCCGCACTGATTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGttt                             <  1:372532/141‑1 (MQ=255)
                                                                                                             tttGAACTACAAGGAACGCCGCACTGATTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGttt                             <  1:372613/141‑1 (MQ=255)
                                                                                                             tttGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGttt                             <  1:651831/141‑1 (MQ=255)
                                                                                                             tttGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGGGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGttt                             <  1:116174/141‑1 (MQ=255)
                                                                                                                     aCAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGAt                     >  1:279908/1‑141 (MQ=255)
                                                                                                                      cAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATa                    >  2:199544/1‑141 (MQ=255)
                                                                                                                      cAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATa                    <  2:319271/141‑1 (MQ=255)
                                                                                                                       aaGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATAc                   <  1:98120/141‑1 (MQ=255)
                                                                                                                         ggAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCAGCAAACATCCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACat                 <  1:522122/141‑1 (MQ=255)
                                                                                                                             cgccgcACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGc             <  2:504852/141‑1 (MQ=255)
                                                                                                                              cccgcACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGCt            <  1:51992/140‑1 (MQ=255)
                                                                                                                                   actgactgACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGCTTggta       >  2:740335/1‑139 (MQ=255)
                                                                                                                                   actgactgACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGCTTGGAt       >  1:722422/1‑141 (MQ=255)
                                                                                                                                     tgactgacCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGCTTGGATaa     <  2:656026/141‑1 (MQ=255)
                                                                                                                                     tgacggagCAGTACCAACCAATTGCTTATGCTGAGCTTAATTAAATCCAGATAATCATAATTTGTGAAATGACCATCATCATGTAATCCATTTGATGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGCTTGGATaa     <  2:313614/141‑1 (MQ=255)
                                                                                                                                        ctgacCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGCTTGGATAAAGt  >  1:42968/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                            
TTTTTGCCTTTTTTATTGCTGCCGCCGTTGTGCTCTCTTTCATACGCTTCAATGAAAGGTTGTACTTCTTTTTTAGCGACTTTTTCATAATAAATGTTCGCAAAGTGTGTTTGAACTACAAGGAACGCCGCACTGACTGACCAGTACAAACCAAGTGCTGATGCTGAGCTTAATGAAATCCAGATAATCATAATTGGTGAAATGACCATCATCATGTAACCCATTTGACGTTGTTCGTCTGGCATCGTTTTACTTGATACATATGCTTGGATAAAGT  >  CP000730/2211854‑2212130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑