Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,816,469 G→C 15.2% Y620* (TAC→TAG USA300HOU_2654 ← serine‑threonine rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,816,4690GC15.2% 220.6 / 21.7 76Y620* (TAC→TAGUSA300HOU_2654serine‑threonine rich protein
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base C (8/4);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGG  >  CP000730/2816331‑2816600
                                                                                                                                          |                                                                                                                                   
tGTTGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGCTTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTa                                                                                                                                   <  1:747421/141‑1 (MQ=255)
 gTTGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGGCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTaa                                                                                                                                  >  1:400608/1‑141 (MQ=255)
  ttGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAAc                                                                                                                                 <  2:523199/141‑1 (MQ=255)
   tGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACt                                                                                                                                >  2:714298/1‑141 (MQ=255)
     gTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTa                                                                                                                              <  1:248161/141‑1 (MQ=255)
     gTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTa                                                                                                                              <  2:676488/141‑1 (MQ=255)
       gCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAAt                                                                                                                            >  2:617569/1‑141 (MQ=255)
       gCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGACAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCATTGCACTATCCTAACTTAAt                                                                                                                            >  1:103033/1‑141 (MQ=255)
             gTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCtt                                                                                                                      <  2:105498/141‑1 (MQ=255)
             gTATCCACAACATTTATTGTAAAAGTTGTCGGCGATTTGTTATTTGCTTGGTCCGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCtt                                                                                                                      <  1:268756/141‑1 (MQ=255)
             gTATCCACAACATTTATTGGAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCtt                                                                                                                      <  2:22936/141‑1 (MQ=255)
                   ccaagtttgatttgaaaaGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAAt                                                                                                                <  2:372959/127‑1 (MQ=255)
                   acaacaTTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAAt                                                                                                                <  2:373529/141‑1 (MQ=255)
                     aacaTTGATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTTCACTATCGTAACTTAATCCGCTTGGTAATcc                                                                                                              <  1:107973/141‑1 (MQ=255)
                      acaTGTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCt                                                                                                             <  2:761716/141‑1 (MQ=255)
                        aTTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGt                                                                                                           >  2:239178/1‑141 (MQ=255)
                           tATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGGACTATCCTCACTTAATCCGCTTGGTAATCCTGTAAc                                                                                                        >  1:624552/1‑141 (MQ=255)
                           tATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAAc                                                                                                        >  2:359454/1‑141 (MQ=255)
                               gTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTa                                                                                                    <  1:113369/141‑1 (MQ=255)
                                 aaaaGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTAtt                                                                                                  >  2:468301/1‑141 (MQ=255)
                                 aaaaGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCAGCTTGGTAATCCTGTAACTGTAtt                                                                                                  >  1:378942/1‑141 (MQ=255)
                                   aaGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTg                                                                                                >  1:61108/1‑141 (MQ=255)
                                   aaGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTg                                                                                                <  1:482295/141‑1 (MQ=255)
                                        gtcgtcGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTcaca                                                                                           >  2:112775/1‑141 (MQ=255)
                                        gtcgtcGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTcaca                                                                                           >  1:257801/1‑141 (MQ=255)
                                        gtcgtcGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGGTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTcaca                                                                                           <  1:711272/141‑1 (MQ=255)
                                            ccgATTCGTCATTTGCTTGCTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGCAATCCTGTAACTGTATTTGTCACAGTcc                                                                                       <  2:687565/140‑1 (MQ=255)
                                                tttGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGt                                                                                   >  2:592114/1‑141 (MQ=255)
                                                  tGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATACGCTTGGTAATGCTGTAACTGTATTTTTTAAAGTCCCAGTaa                                                                                 >  1:252410/1‑140 (MQ=255)
                                                   gTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAGTGATTGACTTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGCATTTGTCACAGTCCCAGTAcc                                                                                >  2:536956/1‑141 (MQ=255)
                                                     tATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCAt                                                                              <  1:83644/141‑1 (MQ=255)
                                                     tATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCAt                                                                              >  1:416210/1‑141 (MQ=255)
                                                     tATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGCATTTGTCACAGTCCCAGTACCAt                                                                              >  1:479408/1‑141 (MQ=255)
                                                     tATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTAGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTCATCCTGTAACTGTATTTGTCACTGTCCCAGTACCAt                                                                              >  1:324734/1‑141 (MQ=255)
                                                       tgtttttGGTCTGTAGACACAACTGTCGATGTTGATTGACCAATTTTTGTTCGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCGCTGCAGGTGTTTGTCACAGTCCCAGTACCATTa                                                                            <  2:502549/136‑1 (MQ=255)
                                                            ttGGGCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGt                                                                       <  1:226573/141‑1 (MQ=255)
                                                             tGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGtt                                                                      <  1:522579/141‑1 (MQ=255)
                                                             tGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGtt                                                                      >  1:48454/1‑141 (MQ=255)
                                                             tGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTATAACTGTATTTCTAACAATCCCAGttgctagattcctt                                                                      >  2:490320/1‑128 (MQ=255)
                                                                 cAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTTTTTGTCGCAGTCCCAGTACCATTATCCGTTGTAg                                                                  >  1:212318/1‑141 (MQ=255)
                                                                   gTAGACCCAACTGCCACTGTTGATTTACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGCTAATCCTGTAACTGTATTTGTCACAGTCACCGTACCATTATCCGTTGTAGTc                                                                <  2:292163/141‑1 (MQ=255)
                                                                   gTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTc                                                                <  1:761660/141‑1 (MQ=255)
                                                                     aGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCATTACCATTATCCGTTGTAGTcaa                                                              >  2:532115/1‑141 (MQ=255)
                                                                     aGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaa                                                              >  2:208455/1‑141 (MQ=255)
                                                                     aGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcta                                                              >  2:743917/1‑139 (MQ=255)
                                                                       acacAACTTTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaata                                                            <  1:411556/141‑1 (MQ=255)
                                                                            aCTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatacaata                                                       <  2:547542/141‑1 (MQ=255)
                                                                               gTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGa                                                    <  1:614114/141‑1 (MQ=255)
                                                                               gTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGa                                                    >  1:154620/1‑141 (MQ=255)
                                                                                tCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGAt                                                   >  1:242650/1‑141 (MQ=255)
                                                                                      ttgattgaCCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCGTTg                                             <  2:257801/141‑1 (MQ=255)
                                                                                       tgattgaCCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGt                                            >  1:572193/1‑141 (MQ=255)
                                                                                          ttgaCCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGtttt                                         <  1:259997/141‑1 (MQ=255)
                                                                                            gaCCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTCACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTAc                                       >  2:189997/1‑141 (MQ=255)
                                                                                                  tttttGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCCTTGTCCGTTGTAGTCAATACAATAGGACTGATTGTTTTACCCCCtt                                 >  1:628528/1‑141 (MQ=255)
                                                                                                   ttttCTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTGACCCACttt                                >  2:527187/1‑140 (MQ=255)
                                                                                                       gttgGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATg                            <  2:244698/141‑1 (MQ=255)
                                                                                                       gttgGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATg                            >  1:73871/1‑141 (MQ=255)
                                                                                                        ttgGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCGCTTCTATgg                           >  2:367294/1‑141 (MQ=255)
                                                                                                        ttgGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATgg                           <  1:468301/141‑1 (MQ=255)
                                                                                                        ttgGTGTCCCAATGATTGAATGCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATgg                           <  2:678479/141‑1 (MQ=255)
                                                                                                          ggTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGtt                         >  1:592917/1‑141 (MQ=255)
                                                                                                          ggTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGtt                         >  1:563288/1‑141 (MQ=255)
                                                                                                          ggTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGtt                         >  1:131683/1‑141 (MQ=255)
                                                                                                          ggTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGtt                         <  2:400608/141‑1 (MQ=255)
                                                                                                           gtgtCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGtttt                        >  1:51469/1‑141 (MQ=255)
                                                                                                                ccAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTCTGGTTTGATTg                   >  1:326/1‑141 (MQ=255)
                                                                                                                   aTGAGTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCt                <  2:561290/141‑1 (MQ=255)
                                                                                                                       ttGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtacag            <  2:61108/141‑1 (MQ=255)
                                                                                                                       ttGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTGCCATTATCCTTTGTAGTCAATACAATAGGATTCATAGTTTTACCCACTTCTATGGGTTGATTGCCtacag            >  1:91495/1‑141 (MQ=255)
                                                                                                                          aaTTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtacagtta         >  1:203985/1‑141 (MQ=255)
                                                                                                                           aTTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtacagttac        <  1:383781/141‑1 (MQ=255)
                                                                                                                            ttCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtacagttaca       <  1:239178/141‑1 (MQ=255)
                                                                                                                            ttCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACCATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtacagttaca       <  2:103033/141‑1 (MQ=255)
                                                                                                                              cGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtacagttacagt     >  2:697819/1‑141 (MQ=255)
                                                                                                                                 tGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTgg  <  1:463886/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                   
TGTTGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGG  >  CP000730/2816331‑2816600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: