Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 807,437:1 +T 10.5% intergenic (‑635/‑166) USA300HOU_0753 ← / → nrdI DMT superfamily drug/metabolite transporter/ribonucleotide‑diphosphate reductase subunit gamma

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730807,4361.T10.5% 222.6 / 22.1 57intergenic (‑634/‑167)USA300HOU_0753/nrdIDMT superfamily drug/metabolite transporter/ribonucleotide‑diphosphate reductase subunit gamma
Reads supporting (aligned to +/- strand):  ref base . (17/34);  new base T (2/4);  total (19/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

AAGTAATTTAACACAGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCATTTTTTAATTGGAATGACTACTTTATTT  >  CP000730/807297‑807574
                                                                                                                                            |                                                                                                                                           
aaGTAATTTAACACAGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGctact                                                                                                                                            >  1:818353/1‑141 (MQ=255)
 aGTAATTTAACACAGAAATTTAACAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGctacta                                                                                                                                           <  1:210351/141‑1 (MQ=255)
  gTAATTTAACACAGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTAt                                                                                                                                          <  1:268456/141‑1 (MQ=255)
     aTTTAACACAGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTAtttt                                                                                                                                       <  2:862949/141‑1 (MQ=255)
        tAACAGAGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTtctc                                                                                                                                    <  1:13484/141‑1 (MQ=255)
          acacAGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTTATTTTCTCa                                                                                                                                   >  2:223607/1‑141 (MQ=255)
          acacAGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTTATTTTCTCa                                                                                                                                   >  1:348338/1‑141 (MQ=255)
            acaGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCGGAACTCATAAGCTACTATTTTCTCAAat                                                                                                                                <  1:418197/141‑1 (MQ=255)
            acaGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAat                                                                                                                                <  2:335395/141‑1 (MQ=255)
             caGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAata                                                                                                                               <  1:84456/141‑1 (MQ=255)
             aaGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAata                                                                                                                               <  1:464392/140‑1 (MQ=255)
               gAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAAtata                                                                                                                             <  2:148654/141‑1 (MQ=255)
                     tAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTa                                                                                                                       <  1:247471/141‑1 (MQ=255)
                              ttatCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTgg                                                                                                             <  1:289710/141‑1 (MQ=255)
                              ttatCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTgg                                                                                                             <  1:463854/141‑1 (MQ=255)
                              atatCAATTAATAGTCAAATTTTTAGAAAATGTACCGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACGATTTTCTAAAAAATAAATCTAAGCAAttcg                                                                                                             <  2:794890/140‑3 (MQ=255)
                               tatCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGt                                                                                                            <  1:830876/141‑1 (MQ=255)
                                    aTTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACa                                                                                                       <  2:51694/141‑1 (MQ=255)
                                    aTTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACa                                                                                                       <  2:824106/141‑1 (MQ=255)
                                     ttAATAGTCATATTTTTTGTAAAAGTACGGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTTTGCACTTGGTTTAcct                                                                                                      >  2:837097/1‑139 (MQ=255)
                                        aTAGTCATATTAATAGAAAATATAATGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTTTTTTTTTTATTCATATAAGATAATATTATACGAAATTGATTTCTTTGCAATTGGTTTACATTTg                                                                                                   >  2:178380/1‑141 (MQ=255)
                                           gTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGATACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTgaga                                                                                                <  2:568305/141‑1 (MQ=255)
                                            tCATCTTTATAGAAAATGAACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTATCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGaa                                                                                               <  1:272339/141‑1 (MQ=255)
                                              atatTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGaaaa                                                                                             <  1:810669/141‑1 (MQ=255)
                                                atTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATa                                                                                           <  2:20078/141‑1 (MQ=255)
                                                     tAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTTATTTTCTCAAATGTAAACCAATTGCATAGATTTATATTTGAGAAAATagtag                                                                                       <  2:434587/141‑5 (MQ=18)
                                                     tAGAAAATGTACTGAGCAAATGGAAGATATCAAATGATGTAAACACTACATATAGTGATTTTTATACATTGAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTat                                                                                      >  2:330610/1‑140 (MQ=255)
                                                        aaaaTGTACTGAGCAAATGGAAGATATCCAATGATGTAAAGACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTTATTTTCTCAAATGTAAACCAATTGCATAGATTTATATTTGAGAAAATAGTAGCtt                                                                                   <  2:441467/141‑1 (MQ=17)
                                                            tGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTTATTTTCTCAAATGTAAACCAATTGCATAGATTTATATTTGAGAAAATAGTAGCttatat                                                                               <  1:223607/141‑5 (MQ=17)
                                                            tGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTTATTTTCTCAAATGTAAACCAATTGCATAGATTTATATTTGAGAAAATAGTAGCttatat                                                                               <  2:348338/141‑5 (MQ=17)
                                                             gTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATta                                                                              <  2:328927/141‑1 (MQ=255)
                                                             gTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATta                                                                              <  2:161864/141‑1 (MQ=255)
                                                                cTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAg                                                                           <  2:755993/141‑1 (MQ=255)
                                                                  gAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGtt                                                                         <  1:147758/141‑1 (MQ=255)
                                                                  gAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAAGAAGTAGCTTCATTATAGtt                                                                         >  2:669842/1‑141 (MQ=255)
                                                                    gCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATACATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTaa                                                                       <  2:818353/141‑1 (MQ=255)
                                                                      aaaTGGAATATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATa                                                                     <  1:236094/141‑1 (MQ=255)
                                                                          ggAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATCTAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCCTTATAGTTAATACAAt                                                                 >  1:150166/1‑141 (MQ=255)
                                                                          ggAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAAt                                                                 >  1:8384/1‑141 (MQ=255)
                                                                           gAAGATATCCAATGATGTAAACACTACATCTAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCTTTATATTTAATACAAtc                                                                >  2:486913/1‑140 (MQ=255)
                                                                            aaGATCTCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGc                                                               <  2:466276/141‑1 (MQ=255)
                                                                                tatCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCGATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTgag                                                           <  1:163415/141‑1 (MQ=255)
                                                                                    cAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTCACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATaa                                                       <  2:688414/141‑1 (MQ=255)
                                                                                       tgatgTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTAAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCa                                                    >  2:803634/1‑141 (MQ=255)
                                                                                            gaaaCACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTa                                               <  1:289559/140‑1 (MQ=255)
                                                                                            aaaaCACAACATAGAGAGATTTTGAAACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTa                                               <  1:34242/140‑1 (MQ=255)
                                                                                                cacTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCa                                           <  2:171073/141‑1 (MQ=255)
                                                                                                    aCATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCAtgtt                                       >  1:318046/1‑141 (MQ=255)
                                                                                                       tataGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATgttgtt                                    >  2:680651/1‑141 (MQ=255)
                                                                                                         taGTGATTTTTATACATTCATCCCATATAAGCTACTATTTTCTCAAATATAAATCCATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACATTGCTGAGATAACCATAGTAACCATGTTGTTaa                                  <  2:313160/141‑1 (MQ=255)
                                                                                                               tttttATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCAtt                            >  2:819912/1‑141 (MQ=255)
                                                                                                               tttttATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCAtt                            >  2:369884/1‑141 (MQ=255)
                                                                                                               tttttATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCAtt                            >  1:787049/1‑141 (MQ=255)
                                                                                                               tttttATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAACAtt                            >  1:19685/1‑141 (MQ=255)
                                                                                                                  ttATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAAGCATGTTGTTAAAGCAttttt                         <  1:524353/141‑1 (MQ=255)
                                                                                                                   tataCATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCAtttttt                        >  1:431237/1‑141 (MQ=255)
                                                                                                                     taCATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCATTTTTTaa                      >  1:314582/1‑141 (MQ=255)
                                                                                                                     taCATTCAACCCATATAAGCTACTATGTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATCAGTAGCTTCATTATAGTTAATAGAATGCAGAGATAACCATAGTAACAATGTGGTTAAAGCATTTTTTaa                      >  2:439946/1‑141 (MQ=255)
                                                                                                                            aaCCCATAGAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTCAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCATTTTTTAATTGGAAt               <  2:374949/141‑1 (MQ=255)
                                                                                                                                         tactaTTTTCTCAAATATAAATCTATGCAATTGGGTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCATTTTTTAATTGGAATGACTACtttattt  <  2:612210/141‑1 (MQ=255)
                                                                                                                                         tacgaTTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCATTTTTTAATTGGAATGACTACtttattt  <  2:608898/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
AAGTAATTTAACACAGAAATTTAATAGTTATTATCAATTAATAGTCATATTTTTAGAAAATGTACTGAGCAAATGGAAGATATCCAATGATGTAAACACTACATATAGTGATTTTTATACATTCAACCCATATAAGCTACTATTTTCTCAAATATAAATCTATGCAATTGGTTTACATTTGAGAAAATAAGTAGCTTCATTATAGTTAATACAATGCTGAGATAACCATAGTAACCATGTTGTTAAAGCATTTTTTAATTGGAATGACTACTTTATTT  >  CP000730/807297‑807574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: