Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,909,490:1 +T 100% coding (297/303 nt) USA300HOU_1771 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,909,4861.T100.0% 194.0 / ‑6.5 63G98V (GGT→GTT) USA300HOU_1771hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base T (28/33);  minor base A (0/1);  total (28/35)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCATAACTATGC  >  CP000730/1909347‑1909621
                                                                                                                                            |                                                                                                                                        
ttGTCTATAATTTTTGCAGATCGTTCAATGAAATCACGAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGt                                                                                                                                         <  1:113873/141‑1 (MQ=255)
gtGTCTATAATTTTTGCAGGTCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGt                                                                                                                                         <  2:211608/140‑1 (MQ=255)
     tataATTTTTGCAGATCGTTCAATTAAATCACTAAAGTCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGGCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGttttta                                                                                                                                   <  2:366759/141‑3 (MQ=255)
       taATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGtttttatt                                                                                                                                 >  1:5796/1‑137 (MQ=255)
         aTTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTtttattta                                                                                                                                >  1:143584/1‑141 (MQ=255)
          tttttGCAGATCGTTCAATTAAATCACTAAGGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTaa                                                                                                                               <  2:36934/141‑1 (MQ=255)
                 aGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAAAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGttttttttttttttagtt                                                                                                                       >  2:461375/1‑131 (MQ=255)
                   gtCGTTCAATTAAATCACTATAGCCTGATGTTGATTTGATTAGTTTTGCGAGTATTATTTTGCCTTGGATTTTTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTaa                                                                                                                      <  2:244774/140‑1 (MQ=255)
                         cAAGTAAATCACTAAAGCCTGATGCAGATTTGACTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTCTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTc                                                                                                                <  1:408654/141‑1 (MQ=255)
                           aTTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAATAAAGTTAATATGTCAt                                                                                                              >  1:367386/1‑141 (MQ=255)
                                 tCACTAAAGCCTGATTCAGATTGGATTAGAATTGCGAGTAAAAGTTTGACTTAGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCCTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGc                                                                                                        <  2:474708/141‑1 (MQ=255)
                                   aCTAAAGCCCGATGCAGATTGGATTAGAATTGAGCTTGCTTTGGTTCTTTGGCTTGATCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGcat                                                                                                      >  1:633903/1‑139 (MQ=255)
                                    cTAAAGCTTGATGCAGATTTGATTAGAATTGCGAGTAAAAGTTTGCCTTCGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                     <  2:857149/141‑1 (MQ=255)
                                    cTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                     <  2:170726/141‑1 (MQ=255)
                                    cTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                     <  2:703428/141‑1 (MQ=255)
                                    cTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCtt                                                                                                     <  2:330433/141‑1 (MQ=255)
                                       aaGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAAt                                                                                                  >  2:864816/1‑141 (MQ=255)
                                       aaGCCTGATGCAAATTGGATGATAATTTCGAGTAAAAGGTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAAt                                                                                                  >  2:725961/1‑141 (MQ=255)
                                         gcatGATGCAGATTGGATTAGGATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATAc                                                                                                <  1:405416/138‑1 (MQ=255)
                                                gCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAacaa                                                                                         <  1:93443/141‑1 (MQ=255)
                                                 tcgATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGATAAAAAAAAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAacaat                                                                                        <  2:436288/139‑1 (MQ=255)
                                                 cAGATTGAATTAGAATTGCGAGTAAATGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAacaat                                                                                        <  2:725757/141‑1 (MQ=255)
                                                     ttGGATTAGAATTGCGTGTAAAAGTTTGACTTGGATTATTCTAAAACTTGATAAATAACATAGCTTTCCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAacaatacaa                                                                                    <  1:374762/141‑1 (MQ=255)
                                                     ttGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAacaatacaa                                                                                    <  1:652507/141‑1 (MQ=255)
                                                       ggATGAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATg                                                                                  <  1:78431/141‑1 (MQ=255)
                                                               aTTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGc                                                                          <  2:143584/141‑1 (MQ=255)
                                                                  gCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCCTGCtat                                                                       >  1:356511/1‑141 (MQ=255)
                                                                      gTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACATTACAATGTATCATTCTATAATg                                                                   >  2:646668/1‑141 (MQ=255)
                                                                       tAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGa                                                                  <  1:13089/141‑1 (MQ=255)
                                                                       tAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGa                                                                  <  1:13307/141‑1 (MQ=255)
                                                                         aaaGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGt                                                                <  1:143241/141‑1 (MQ=255)
                                                                         aaaGTTTGCCTTGGATTATTCTAATTGTTATTTTAGGACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGt                                                                <  2:827531/141‑1 (MQ=255)
                                                                          aaGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTa                                                               <  1:320361/141‑1 (MQ=255)
                                                                            gTTTGCCTTTGATTATTCTCATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTaaa                                                             <  2:844654/141‑1 (MQ=255)
                                                                                  cTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAAtt                                                       <  1:222120/141‑1 (MQ=255)
                                                                                  cTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATAATGCTATAATGAGTAAAACAAtt                                                       >  2:668070/1‑141 (MQ=255)
                                                                                        ttattCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATaa                                                 <  1:83570/141‑1 (MQ=255)
                                                                                        ttattCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGGAAAACAATTTGCTaa                                                 >  1:788447/1‑141 (MQ=255)
                                                                                         tattCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATAATGCTATAATTAGTAAAACAATTTGATaaa                                                >  2:658641/1‑140 (MQ=255)
                                                                                           ttCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTCAATAACgt                                              >  2:174249/1‑141 (MQ=255)
                                                                                                    ttATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGta                                     >  1:865959/1‑141 (MQ=255)
                                                                                                     tATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTGTTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGtat                                    >  2:460811/1‑141 (MQ=255)
                                                                                                      aTTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGtata                                   <  2:43809/141‑1 (MQ=255)
                                                                                                       ttttAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATaa                                  >  1:477231/1‑141 (MQ=255)
                                                                                                         ttAGTACATATCTTTTCACGTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTTTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATATTATAACGTTGTTGCGTATaaaa                                <  2:379224/141‑1 (MQ=255)
                                                                                                         ttAGTACATATCTTTTAAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATaaaa                                <  1:21536/141‑1 (MQ=255)
                                                                                                            gTACATATCTTTTCTATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAat                             >  1:565534/1‑141 (MQ=255)
                                                                                                              gcacatCTTTCGAAGTGGTCGGACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAatat                           <  1:820585/137‑1 (MQ=255)
                                                                                                              acataccggtccAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATCACGTTGTTGCGTATAAAAAatat                           <  1:820551/130‑1 (MQ=255)
                                                                                                               cATATCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATAtt                          >  2:850284/1‑141 (MQ=255)
                                                                                                                  atCTTTTTAATTCTTCGTACATTCGATATATATATAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGa                       <  2:507591/141‑1 (MQ=255)
                                                                                                                  atCTTTTCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGa                       >  2:597378/1‑141 (MQ=255)
                                                                                                                      tttCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGAtttt                   <  1:725961/141‑1 (MQ=255)
                                                                                                                      tttCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGAtttt                   >  1:270284/1‑141 (MQ=255)
                                                                                                                      tttCAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGAtttt                   <  1:864816/141‑1 (MQ=255)
                                                                                                                         cAATTGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGa                >  2:243175/1‑141 (MQ=255)
                                                                                                                            ttGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAAt             >  1:285093/1‑141 (MQ=255)
                                                                                                                            ttGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAAt             <  1:293950/141‑1 (MQ=255)
                                                                                                                            ttGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAAt             >  2:752290/1‑141 (MQ=255)
                                                                                                                            ttGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAAt             >  1:654984/1‑141 (MQ=255)
                                                                                                                            ttGTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAAt             >  2:752245/1‑141 (MQ=255)
                                                                                                                              ttgcggaCATTCGGTTTTTATTTAAAAAAGGTAATATGTCATTGTCGCCTTAATACAAAACAATACAATGTATAATGCTATAATGAGTAAAACAATTTGATACCGTTGTTGCGTATAAAAAATATTAGCTTTTCGAAATCa           >  2:224283/7‑141 (MQ=255)
                                                                                                                              gTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGGTAACGTTGTTGCGTATAAAAAATATTCGATTTTCGCAAGCa           >  1:107674/1‑141 (MQ=255)
                                                                                                                              gTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCa           <  2:380300/141‑1 (MQ=255)
                                                                                                                              gTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGGAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCa           >  2:64560/1‑141 (MQ=255)
                                                                                                                              gTTCGTACATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCAAGCTATAATGAGTAAAAAAATTTGATAACGTGGTTGCGTATAAAAAATATTAGATTTTCGAAATCa           >  2:115143/1‑141 (MQ=255)
                                                                                                                                     cATTCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTAACATGCTATAATGAGTAAAACAATTTGATAACGTTGTTACGTATAAAAAATATTAGATTTTCGAAATCATAAatat    >  2:654804/1‑141 (MQ=255)
                                                                                                                                       ttCGGTTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCATAACTATGc  <  1:243175/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
TTGTCTATAATTTTTGCAGATCGTTCAATTAAATCACTAAAGCCTGATGCAGATTGGATTAGAATTGCGAGTAAAAGTTTGCCTTGGATTATTCTAATTGTTATTTTAGTACATATCTTTTCAATTGTTCGTACATTCGGTTTTATTTAAAAAAGTTAATATGTCATTGTAGCCTTAATACAAAACAATACAATGTATCATGCTATAATGAGTAAAACAATTTGATAACGTTGTTGCGTATAAAAAATATTAGATTTTCGAAATCATAACTATGC  >  CP000730/1909347‑1909621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: