Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	264,463	G→A	5.5%	W226* (TGG→TGA)	glpQ1 →	possible glycerophosphodiester phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	264,463	0	G	A	5.5%	215.1 / 3.4	76	W226* (TGG→TGA)	glpQ1	possible glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (31/39);  new base A (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.21e-01

TATGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGGAGATAAGTTTTT  >  CP000730/264325‑264603                                                                                                                                           |                                                                                                                                             tATGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACGGCCAGTCGTTATTTAAAAATATGAGACGAAGATGGCa                                                                                                                                            >  2:403713/1‑141 (MQ=255) tATGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCGAGTCGTTGTTTAAAAATATGAGAATAAGTTGGCa                                                                                                                                            >  2:267737/1‑141 (MQ=255) tATGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTggga                                                                                                                                            >  2:276098/1‑139 (MQ=255) tATGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCa                                                                                                                                            <  1:3826/141‑1 (MQ=255)  aTGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTGTTTAAAAATATGAGACTACGTTGGCaa                                                                                                                                           >  1:62848/1‑141 (MQ=255)   tGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCaaa                                                                                                                                          <  2:39786/141‑1 (MQ=255)   tGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCaaa                                                                                                                                          <  1:799800/141‑1 (MQ=255)   tGAAAACGACGAAAGGTATAATCATTTACGGTACCTATATGATTGCTGTACTTTTATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCaaa                                                                                                                                          >  1:335580/1‑141 (MQ=255)            cgaAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAGTATAAGACTAAGTTGGCAAATTCCGAAGc                                                                                                                                 >  2:64308/1‑141 (MQ=255)              aaaGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGa                                                                                                                               >  1:100778/1‑141 (MQ=255)              aaaGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGa                                                                                                                               <  2:83965/141‑1 (MQ=255)               aaGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGaa                                                                                                                              >  1:274102/1‑141 (MQ=255)                aGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATTAGACTGAGTGGGCAGATTACGAAGCGaaa                                                                                                                             >  1:498154/1‑141 (MQ=255)                aGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACCAAGCGaaa                                                                                                                             >  2:40835/1‑141 (MQ=255)                aGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGACAAATTACGAAGCGaaa                                                                                                                             >  1:100796/1‑141 (MQ=255)                aGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGACAAATTACGAAGCGaaa                                                                                                                             >  1:100910/1‑141 (MQ=255)                aGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTATAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGaaa                                                                                                                             >  2:639069/1‑141 (MQ=255)                  gTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATa                                                                                                                           <  2:405821/141‑1 (MQ=255)                          aTTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCgg                                                                                                                   <  1:861543/141‑1 (MQ=255)                              aCGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTGAAAATCTAGGACTAAGTTGGCAAATTACGAAGAGACATAAGTTTGGGCtgg                                                                                                               >  1:763959/1‑139 (MQ=255)                                   aCCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGATTGTtata                                                                                                          >  1:438014/1‑141 (MQ=255)                                    ccTTTATGATTTATGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCTCCAGTCGTTATTTAAAAATATGAGACCAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAg                                                                                                         <  2:199918/141‑1 (MQ=255)                                    ccTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAg                                                                                                         >  2:751900/1‑141 (MQ=255)                                      tttATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGaa                                                                                                       >  1:262548/1‑141 (MQ=255)                                       ttATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGaaa                                                                                                      <  1:133933/141‑1 (MQ=255)                                               gCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTtata                                                                                              <  2:91869/141‑1 (MQ=255)                                                 tGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGAATAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATAtt                                                                                            <  2:780950/141‑1 (MQ=255)                                                     tttATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGaa                                                                                        >  1:514409/1‑141 (MQ=255)                                                      ttATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAAc                                                                                       <  1:312391/141‑1 (MQ=255)                                                      ttATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAAc                                                                                       <  1:299342/141‑1 (MQ=255)                                                       tataTTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCAAAATAAGTTTCGGCTTGTTATAGAAACAGTTATATTAGAACt                                                                                      >  2:600981/1‑141 (MQ=255)                                                           ttaaattttaaattAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATACGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACtcatc                                                                                  >  2:439045/1‑141 (MQ=255)                                                            taaattttaaattAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACtcatca                                                                                 <  2:486181/141‑1 (MQ=255)                                                              aattttaaattAATATTTATTTTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCAtt                                                                               <  2:59361/141‑1 (MQ=255)                                                                ttttaaattAAAATTTACCCTACCGGTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTgg                                                                             <  1:318714/141‑1 (MQ=255)                                                                 tttaaattAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCCTCATTGGt                                                                            >  1:352927/1‑141 (MQ=255)                                                                   tacattattattTTCTCTACCTTTAACGATTTTAAACCGCCAGTCGTTATTTAAACATATGCGACTAAGCTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGc                                                                          <  2:127380/138‑1 (MQ=255)                                                                    aaattAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAGATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCg                                                                         >  1:577013/1‑141 (MQ=255)                                                                     actcaaTATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTGATTTAAAAATATGAGACTAAGCTGGCAAATTACGAAGCGAAATAAGTTTCTGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGa                                                                        <  2:498154/137‑1 (MQ=255)                                                                     aattAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGCAATAAGTTTCGGCTGGTTATAGAAATAGTTATATTAGAACTCATCGTTGTTGCGa                                                                        >  1:142151/1‑141 (MQ=255)                                                                     aattAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGa                                                                        <  1:639069/141‑1 (MQ=255)                                                                     aattAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGACAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGa                                                                        <  2:100910/141‑1 (MQ=255)                                                                     aattAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGACAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGa                                                                        <  2:100796/141‑1 (MQ=255)                                                                     aattAATATTTACTCTACCGCAAACGATTTTAAACCGCCTGTAGTTATTTAAAAATTCAAGAATAAGTTGTCTAATTACGACTTGAATTAAAATTCCGCTTGTTATAGAAATAGTTATATTTAAACTCATCATTGGTGCgt                                                                        >  2:340380/1‑140 (MQ=255)                                                                       ttAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTCAAAAGAGTGGACTACATTGCCAAGTTACGAAGCGAAATAAGTTTCGGCTTTTTATAGAAATAGTTATATTAGAACTCATCATTGGTGccgtt                                                                      >  1:413904/1‑137 (MQ=255)                                                                         aacatTTACTCTCCCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCGTCATTGGTGCGAtttt                                                                    <  1:481116/138‑1 (MQ=255)                                                                              ttACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACat                                                               >  1:712151/1‑141 (MQ=255)                                                                                tctctACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACatta                                                             <  2:747821/140‑1 (MQ=255)                                                                                tctctACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACatta                                                             <  1:485688/140‑1 (MQ=255)                                                                                 ctctACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACattaa                                                            >  1:78896/1‑141 (MQ=255)                                                                                      acGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAATTATGAGACTAAGTTGGTAAATTACGAAGCGAAATAAGTTTCGGATTGTTATAGAAAGAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAattatt                                                       <  1:62878/140‑1 (MQ=255)                                                                                       cgggagggatgtgaaaCAGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTAttt                                                      <  2:212276/128‑1 (MQ=255)                                                                                       ccttAACGATTTTAGACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTAttt                                                      <  2:212414/139‑1 (MQ=255)                                                                                           aaCGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTAATCATTGGTGAGATTTTAACATTAATTATTTCAgg                                                  >  1:508723/1‑141 (MQ=255)                                                                                           aaCGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGATAATTACATAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAgg                                                  >  1:198891/1‑141 (MQ=255)                                                                                                   tAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACat                                          <  1:42703/141‑1 (MQ=255)                                                                                                   tAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACat                                          <  2:644133/141‑1 (MQ=255)                                                                                                   tAAACCGCCAGTCGTTATTTAAAAAGATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTAGTTCAGGAGCAACat                                          <  2:588528/141‑1 (MQ=255)                                                                                                        cGCCAGTCTTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCtt                                     <  2:164795/141‑1 (MQ=255)                                                                                                        cGCCAGTCGTTATTTGAAAATATGAGACTAAGTTGGCCAATTATGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCAACATTGGTGCGATTTTACCATTAATTATTTCAGGAGCAACATTTCtt                                     <  1:610025/141‑1 (MQ=255)                                                                                                         gCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTg                                    <  1:751900/141‑1 (MQ=255)                                                                                                          ccAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAAACTATCTTGc                                   >  1:169266/1‑141 (MQ=255)                                                                                                          caaGTCGTGATTTAAAAATATGAGACGAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGc                                   <  2:784750/139‑1 (MQ=255)                                                                                                            aGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTAAGAAACGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTa                                 >  2:141717/1‑141 (MQ=255)                                                                                                              tCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTAtt                               >  1:287154/1‑141 (MQ=255)                                                                                                              tCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTGGCTAtt                               >  1:287136/1‑141 (MQ=255)                                                                                                              tCGGTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAAGAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTAtt                               <  2:252569/141‑1 (MQ=255)                                                                                                                gTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTtg                             >  1:26963/1‑141 (MQ=255)                                                                                                                       aaaaaTATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGATATTTGTGTAGAt                      >  2:23582/1‑141 (MQ=255)                                                                                                                          aaTATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATgaa                   >  1:741891/1‑141 (MQ=255)                                                                                                                               gagaCTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAgg              <  1:234052/141‑1 (MQ=255)                                                                                                                                agaCTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGga             <  2:577013/141‑1 (MQ=255)                                                                                                                                 gaCTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATATAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGgag            <  1:514021/141‑1 (MQ=255)                                                                                                                                        ttGGGAAATTCCGAAGTGAAATAAGTTGCGGATTGTTATAGAAATAGTTATATTATTACTCATTATTGGTGCGATTTTAGCATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGGAGATAAGtt     <  2:817800/141‑1 (MQ=255)                                                                                                                                        ttGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGGAGATAAGtt     <  2:854272/141‑1 (MQ=255)                                                                                                                                        gtGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGGAGATAAGtt     <  2:699212/140‑1 (MQ=255)                                                                                                                                         tGGCAAAGGACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGGAGATAAGttt    <  1:153961/141‑1 (MQ=255)                                                                                                                                           gCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGACCTCATCATTGGTGCGATTTTAACATTAATTATTTCATGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGGAGATAAGttttt  >  1:33845/1‑141 (MQ=255)                                                                                                                                           gCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACAGTAATTATTGCAGGAGCAACATATATTGCTATTTGTGTAGATGAGGAAGGAGATAAGttttt  >  1:820840/1‑141 (MQ=255)                                                                                                                                           gCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTGTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGGAGATAAGttttt  >  1:33686/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                             TATGAAAACGACGAAAGGTATAATCATTTACGGTACCTTTATGATTGCTGTATTTATATTAAATTTTAAATTAATATTTACTCTACCGTTAACGATTTTAAACCGCCAGTCGTTATTTAAAAATATGAGACTAAGTTGGCAAATTACGAAGCGAAATAAGTTTCGGCTTGTTATAGAAATAGTTATATTAGAACTCATCATTGGTGCGATTTTAACATTAATTATTTCAGGAGCAACATATCTTGCTATTTGTGTAGATGAAGAAGGAGATAAGTTTTT  >  CP000730/264325‑264603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑