Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 535,338 A→T 5.3% intergenic (+4909/‑816) USA300HOU_0494 → / → rplY hypothetical protein/ribosomal protein L25

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730535,3380AT 5.3% 234.0 / 4.0 78intergenic (+4909/‑816)USA300HOU_0494/rplYhypothetical protein/ribosomal protein L25
Reads supporting (aligned to +/- strand):  ref base A (36/36);  new base T (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

AGTATTCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAGGATTCTTTGATA  >  CP000730/535198‑535477
                                                                                                                                            |                                                                                                                                            
agTATTCTGGGTTGTGACGTATTTATTATTCAACCAACATCAGATCCTGGGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGa                                                                                                                                              <  2:99012/141‑1 (MQ=255)
agTATTCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGa                                                                                                                                              <  1:742206/141‑1 (MQ=255)
agTATTCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATACTGTAGTGCCATATTATGGa                                                                                                                                              <  1:388355/141‑1 (MQ=255)
 gTATTCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATCTTGTAGTGCCCTATTATGGaa                                                                                                                                             >  2:171916/1‑140 (MQ=255)
 gTATTCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGat                                                                                                                                             <  1:805528/141‑1 (MQ=255)
 gTATTCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGat                                                                                                                                             <  1:79910/141‑1 (MQ=255)
   tttCGGGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAACTATGATTGATGCTTGTAAACGTGCTTCGGCAGCAACAATCAATATTGTAGTGCCATATTATGGatat                                                                                                                                           <  2:495758/140‑1 (MQ=255)
    ttCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGatatc                                                                                                                                          >  2:402129/1‑140 (MQ=255)
    ttCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATg                                                                                                                                          <  2:94692/141‑1 (MQ=255)
         ggTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGcaaga                                                                                                                                     <  2:839078/141‑1 (MQ=255)
         ggTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGcaaga                                                                                                                                     <  2:131781/141‑1 (MQ=255)
          gTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTTCCATATTATGGATATGcaagac                                                                                                                                    <  2:632877/141‑1 (MQ=255)
            tgtgACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGcaagacaa                                                                                                                                  <  1:145174/141‑1 (MQ=255)
              tgACTTATTTATTATTCTACCAACCTCATTGCCTGTGAATCTACATTTATTGGAATTATTAATTATGATTGATGCTTGTATACGTGCTTCTGCAGCAAACATCAATATTGTAGTGCCATATTATGTATATGcaagacaaga                                                                                                                                <  2:503884/141‑1 (MQ=255)
              tgACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGcaagacaaga                                                                                                                                <  1:120024/141‑1 (MQ=255)
              tgACCTATTTACTATTCAACCAAAATCACTTCCCGTGAATCTTCATCTACTGGAATTATTCAATATGATTGACGCTTGTAAACGTGCTTCTGCAGCGACGATCAATATTGTAGTGCCATATGATGGATATGccagacatgg                                                                                                                                <  2:807663/141‑4 (MQ=255)
                aCGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATa                                                                                                                              <  2:169443/141‑1 (MQ=255)
                   tatttattATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGGTAGaa                                                                                                                           >  1:120595/1‑141 (MQ=255)
                        atttttTAACGAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGAGCTTCAGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGcc                                                                                                                     <  2:647314/141‑1 (MQ=255)
                        attattCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGcc                                                                                                                     <  2:755978/141‑1 (MQ=255)
                        attattCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGcc                                                                                                                     >  1:196699/1‑141 (MQ=255)
                         ttattCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGTAGGTGCAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGccc                                                                                                                    >  1:629111/1‑141 (MQ=255)
                          tattCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCg                                                                                                                   <  1:495266/141‑1 (MQ=255)
                           attCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTCGTCAACGTGCTTCTGCAGCAACACTCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAACGACCGt                                                                                                                  >  2:113494/1‑141 (MQ=255)
                                accaacATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCg                                                                                                             >  1:696654/1‑141 (MQ=255)
                                  caacATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGTTATGCAAGACAAGATAGGAAAGACCGTAGCCGTg                                                                                                           >  2:828140/1‑141 (MQ=255)
                                  caacATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGTTATGCAAGACAAGATAGAAAAGCCCGTAGCCGTg                                                                                                           >  2:388444/1‑141 (MQ=255)
                                    acATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAg                                                                                                         >  2:739408/1‑141 (MQ=255)
                                     ctttatatCCTGTTAAGCAACATATAATGGAAGTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCATCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGc                                                                                                        <  1:389776/137‑1 (MQ=255)
                                       tcatATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATCTTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCTTAGCCGTGAGCCa                                                                                                      <  2:820341/141‑1 (MQ=255)
                                        cctatCCTGTGAATCTAAATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCaa                                                                                                     <  1:191492/139‑1 (MQ=255)
                                        catATCCTGTGAATCTCCATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTCTGGATATGCAAGACAAGACAGAAACGCCCTTAGCAGTGattggc                                                                                                     <  1:474535/141‑6 (MQ=255)
                                           atCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCa                                                                                                  >  1:715767/1‑141 (MQ=255)
                                               ggtgAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGc                                                                                              <  2:687081/140‑1 (MQ=255)
                                                      cTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTa                                                                                       >  2:661335/1‑141 (MQ=255)
                                                      cTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTa                                                                                       >  2:803372/1‑141 (MQ=255)
                                                       tACAATTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCATCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTaa                                                                                      >  2:527002/1‑140 (MQ=255)
                                                         cATTTAATGGAATTATTAATTATGATGGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGtt                                                                                    <  1:300687/141‑1 (MQ=255)
                                                           ttaaaTTGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGc                                                                                  <  1:484302/138‑1 (MQ=255)
                                                            tcagtGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGTCATATTATGGGTATGCAAGACAAGAGAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCa                                                                                 <  2:539649/137‑1 (MQ=255)
                                                                tGGAATTATTAATTATGATTGATGCTTGTAACCGTGCTTCTGCACAAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACt                                                                             <  2:304203/141‑1 (MQ=255)
                                                                tGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACt                                                                             <  1:89678/141‑1 (MQ=255)
                                                                   aaTTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCGGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTaa                                                                          >  2:542059/1‑141 (MQ=255)
                                                                      tattaAATATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCg                                                                       <  1:848974/141‑1 (MQ=255)
                                                                          aattaTGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAAc                                                                   <  1:654357/141‑1 (MQ=255)
                                                                              aTGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCt                                                               <  1:797049/141‑1 (MQ=255)
                                                                                 attgatGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGgc                                                            >  2:633512/1‑141 (MQ=255)
                                                                                 attgatGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGgc                                                            >  2:768389/1‑141 (MQ=255)
                                                                                 attgatGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGgc                                                            >  1:727812/1‑141 (MQ=255)
                                                                                   tgatGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGgcgc                                                          <  1:489753/141‑1 (MQ=255)
                                                                                   tgatGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAAACACTGCTAAATTAGTTGCAAACTTAATCGAAACCGCTGgcgc                                                          <  2:328027/141‑1 (MQ=255)
                                                                                    gatGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCa                                                         >  1:464839/1‑141 (MQ=255)
                                                                                    gatGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCa                                                         >  1:315037/1‑141 (MQ=255)
                                                                                     atGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTAGGGCTATGCAAGACACGATAGAAAAGCCCGTAGCCGTGAGCCAAGCACTGCTAAATTAGATGCACACGTAATCGAAACAGCTGGCGCag                                                        >  2:148646/1‑140 (MQ=255)
                                                                                          tGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGAATCAGACAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGt                                                   <  1:409384/141‑1 (MQ=255)
                                                                                                cGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGAtt                                             >  1:136501/1‑141 (MQ=255)
                                                                                                 gTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTg                                            >  1:366481/1‑141 (MQ=255)
                                                                                                 gTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTg                                            <  1:474895/141‑1 (MQ=255)
                                                                                                  tGCTTCTGCAGCAAGAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGc                                           >  1:452726/1‑141 (MQ=255)
                                                                                                  tGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGATACAGCTGGCGCAACTCGTATGAttac                                           >  1:635587/1‑139 (MQ=255)
                                                                                                        cgcagcaACAATTAATATTGTTGGGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTTCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGa                                     <  1:389582/140‑1 (MQ=255)
                                                                                                          cagcaACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGa                                                                      >  2:782957/1‑106 (MQ=255)
                                                                                                          cagcaACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGa                                                                      <  1:782957/106‑1 (MQ=255)
                                                                                                          cagcaACAATCAATACTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGa                                                                      <  1:782954/106‑1 (MQ=255)
                                                                                                          cagcaACAATCAATACTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGa                                                                      >  2:782954/1‑106 (MQ=255)
                                                                                                            gcaACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTa                                 >  1:802542/1‑141 (MQ=255)
                                                                                                              aacaaTCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGACAGAGAATCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACa                               >  1:378461/1‑141 (MQ=255)
                                                                                                                  atcaatTTTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGca                           <  1:459181/141‑1 (MQ=255)
                                                                                                                    caatATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGACGCAACTCGTATGATTGCGTTGGACTTACATGcacc                         >  2:789509/1‑141 (MQ=255)
                                                                                                                     aatATTGTAGTGCCATATTATGGTTATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGcacca                        <  1:388444/141‑1 (MQ=255)
                                                                                                                     aatATTGTAGTGCCATATGATGGTTATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGcacca                        <  1:828140/141‑1 (MQ=255)
                                                                                                                             aGTGCCATCTTATGGATATGCAATACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCa                <  2:378461/141‑1 (MQ=255)
                                                                                                                             aGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTAGTATGATTGCGTTAAACTTACATGCACCACaacatca                >  2:712544/1‑136 (MQ=255)
                                                                                                                              gTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAGTCGGAACAGCTGGCGCAGCTCGTATGATTGCGTTAGACTTCCATGCACCACACATCCaa               >  2:261651/1‑141 (MQ=255)
                                                                                                                                gCCCTATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCCACTGGTATGATTGAATTCGATGCACCTGCACCAAAAATTCAAgg             >  2:175970/1‑141 (MQ=255)
                                                                                                                                gCCATATTATGGATATGCAAGACAAGATAGAAATGCCCGTAGGCGTGAGCCAATCACGGTTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAgg             <  2:720119/141‑1 (MQ=255)
                                                                                                                                gCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAgg             <  2:766713/141‑1 (MQ=255)
                                                                                                                                  cATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAGGAt           >  1:800270/1‑141 (MQ=255)
                                                                                                                                    tattatGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAGGATTc         >  1:314950/1‑141 (MQ=255)
                                                                                                                                       tatGGAATTGCAAGACAATATAGAAAAGCCCTTACCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAACGATTCttt      <  2:499881/141‑1 (MQ=255)
                                                                                                                                        atGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGGACCCCAAATTCAAGGATTCtttt     >  1:225830/1‑140 (MQ=255)
                                                                                                                                        atGGATATGCAAGACAAGCAAAATGGCGCCGTAGCCGTGAGCCAATCACTGGTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCTTATGATTGCGTTAGACTTACATGCACCACAAATTCAAGGATTCTTTg     >  2:614339/1‑141 (MQ=255)
                                                                                                                                          ggATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGAGTTAGCATAAAATACCCAACAAATTCAAGGATTCTTTGat   >  2:31172/1‑141 (MQ=255)
                                                                                                                                           gATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAGGATTCTTTGata  >  2:856702/1‑141 (MQ=255)
                                                                                                                                           gATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAGGATTATTTGata  >  1:791073/1‑141 (MQ=255)
                                                                                                                                           gATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAGGAGTCTGTGata  >  1:109796/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AGTATTCGTGGTTGTGACGTATTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATGATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGATATGCAAGACAAGATAGAAAAGCCCGTAGCCGTGAGCCAATCACTGCTAAATTAGTTGCAAACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAAATTCAAGGATTCTTTGATA  >  CP000730/535198‑535477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: