Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 607,884 G→A 100% R209* (CGA→TGA)  USA300HOU_0550 ← deoxynucleoside kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730607,8840GA99.7% 286.6 / ‑6.6 93R209* (CGA→TGA) USA300HOU_0550deoxynucleoside kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/54);  minor base C (1/0);  total (39/54)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTATATGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCGAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTT  >  CP000730/607746‑608013
                                                                                                                                          |                                                                                                                                 
gtcgatGGGAATTAATGTATTCGATAGAATTGATAAAATAATTGATAATTTCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAg                                                                                                                                 <  2:377755/137‑1 (MQ=255)
 tataTGTGAATTAAGGTATTCGATAGAATTGTTAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGc                                                                                                                                <  2:228068/141‑1 (MQ=255)
    atggtaaTTAATGTATTCGATAGAATTGATAAAATAATCGATGATATCTATGGTATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAAt                                                                                                                             <  2:89751/136‑1 (MQ=255)
    atGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAAt                                                                                                                             >  1:477803/1‑141 (MQ=255)
    atGTGAATTAATGTACTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAAt                                                                                                                             <  1:323956/141‑1 (MQ=255)
      gtgAATTTATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAAttt                                                                                                                           <  2:736479/141‑1 (MQ=255)
      gtgAATTAAAGTATTCGAAAAAATTGATAAAAAAAACGATAATATCTAAGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGTAAttt                                                                                                                           <  2:43512/141‑1 (MQ=255)
        gAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTg                                                                                                                         <  1:237403/141‑1 (MQ=255)
            tAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTa                                                                                                                     >  2:465592/1‑141 (MQ=255)
              aTGTATTCGATAGAATTGATCAAATAATCGATAATATCTATGGACTATACATTGAATATAAACATATACGCTATGTCTTTAGTCTATCATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATc                                                                                                                   >  2:472227/1‑141 (MQ=255)
               tGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCa                                                                                                                  >  1:400259/1‑141 (MQ=255)
               tGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCa                                                                                                                  >  1:604543/1‑141 (MQ=255)
                gTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTCTCGTGTATCTACTTTTCGATATGTTTGAATAATTCCAGCATTTTTGTTTATCAt                                                                                                                 >  1:362061/1‑141 (MQ=255)
                gTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCAt                                                                                                                 >  2:467091/1‑141 (MQ=255)
                gTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCAt                                                                                                                 >  1:347469/1‑141 (MQ=255)
                gTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTCTTATCGTGCATTTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCAt                                                                                                                 <  2:567495/141‑1 (MQ=255)
                 tATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATa                                                                                                                <  2:29726/141‑1 (MQ=255)
                 gaTTCGAAAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATa                                                                                                                <  2:56679/140‑1 (MQ=255)
                      gATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTTTCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGAtt                                                                                                           >  1:281090/1‑141 (MQ=255)
                      gATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGAtt                                                                                                           >  2:132151/1‑141 (MQ=255)
                       aTAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGAttt                                                                                                          <  2:282956/141‑1 (MQ=255)
                        tAGAGTTGATAAAATAATCGATAATATCTAGGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACGTGTCGATATGTTGGAATAATTCAAGCAATTTTGTTTATCATAGGAtttt                                                                                                         >  2:525136/1‑140 (MQ=255)
                        tAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTa                                                                                                         >  1:494893/1‑141 (MQ=255)
                        tAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATCTTGTTTATCATAGGATTTa                                                                                                         <  1:473899/141‑1 (MQ=255)
                        aagaatgtaaaaaaTAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTa                                                                                                         <  1:726717/131‑1 (MQ=255)
                         aGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTaa                                                                                                        >  1:830340/1‑141 (MQ=255)
                         aGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTaa                                                                                                        <  1:833978/141‑1 (MQ=255)
                         aGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTaa                                                                                                        >  1:811039/1‑141 (MQ=255)
                            aTTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGa                                                                                                     >  1:801507/1‑141 (MQ=255)
                               gATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTc                                                                                                  >  1:651498/1‑141 (MQ=255)
                                 tAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCgg                                                                                                >  2:359379/1‑141 (MQ=255)
                                 aaaaaTAATCGATACTATCTCTTGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCgg                                                                                                <  1:924/140‑1 (MQ=255)
                                    aataatCCAGAATAGCGATGCAAAATACATCGTATATAAACATATTCGCTATGTCTTTAGTCTTTTATCCTGTATCTACCTGTCGACATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAATATTCGGGGt                                                                                             <  2:208545/141‑1 (MQ=255)
                                    aataacCAATAATATCTAAGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGt                                                                                             <  1:95066/141‑1 (MQ=255)
                                       aatCGATAATATTTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCt                                                                                          <  2:222851/141‑1 (MQ=255)
                                        atCGATAATATCTATGGAATATTGATTTAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCtt                                                                                         >  1:207458/1‑141 (MQ=255)
                                         tcaataataTCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTa                                                                                        <  2:6772/138‑1 (MQ=255)
                                         tCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAATGATTCGGGGTCCTTa                                                                                        <  2:311478/141‑1 (MQ=255)
                                            ataataTCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATgg                                                                                     >  1:307531/1‑141 (MQ=255)
                                                tatCTAAGGAATATACATTGAATATAAACATATACGGTATGTCTTTAGTCTTTTAGCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGatat                                                                                 <  2:445275/141‑1 (MQ=255)
                                                  tCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCATTTTTGTTTATCATAGGCTTTAAAGATTCGGGGTCCTTATGGATATct                                                                               >  2:667692/1‑140 (MQ=255)
                                                  tCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGatataa                                                                               >  2:842124/1‑139 (MQ=255)
                                                   cTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCat                                                                              <  1:362689/141‑1 (MQ=255)
                                                    tATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCata                                                                             >  1:692581/1‑141 (MQ=255)
                                                         aaTATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCAt                                                                        <  2:353737/141‑1 (MQ=255)
                                                           tataCATTTAATATAAACATATACGCTATGTCTTTAGTTTTTTATCGTTTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTa                                                                      <  2:115317/141‑1 (MQ=255)
                                                              cccttGAAAATATACATTTACGCTATGTTTTTAGTCTTTTATCGTGTATCTATTTGTCGAGATGTTTGAATAATTCAACCAATTTTCTTTGTCATAGGATTTAAAGATTCGGTGTGCTTATTGATATCATATTCATTAata                                                                   <  2:394090/138‑1 (MQ=255)
                                                              aCATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAata                                                                   <  2:689534/141‑1 (MQ=255)
                                                                  tGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGa                                                               <  2:418262/141‑1 (MQ=255)
                                                                    aaTATAAACATATACGCTATTTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATa                                                             <  2:811039/141‑1 (MQ=255)
                                                                       ataAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGt                                                          <  2:773236/141‑1 (MQ=255)
                                                                        taAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTa                                                         >  2:869662/1‑141 (MQ=255)
                                                                          aaCATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACa                                                       >  1:503889/1‑141 (MQ=255)
                                                                          aaCATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACa                                                       >  1:94828/1‑141 (MQ=255)
                                                                          aaCATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCAGATTCATTAATATTGATACGTACa                                                       >  2:839040/1‑141 (MQ=255)
                                                                             atatACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACt                                                    <  1:670804/141‑1 (MQ=255)
                                                                                   gCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGGGTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATTTTCATTAATATTGATACGTACAACTGGACAt                                              >  2:432220/1‑141 (MQ=255)
                                                                                    cTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATg                                             <  1:465592/141‑1 (MQ=255)
                                                                                     tATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATCTTTAGTTATATTGATACGTACAACTGGACATGc                                            <  2:795639/141‑1 (MQ=255)
                                                                                     tATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACACGc                                            >  2:163108/1‑141 (MQ=255)
                                                                                        gTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGCTATCATATTCATTAATATTGATACGTACAACTGGACATGCAtt                                         <  2:651498/141‑1 (MQ=255)
                                                                                        gTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGAGTTAAAGATTCGGCGTCCTTATGGATATCATATTCATTAACATTGATACGTCCAACTGGACATGCAtt                                         >  1:196361/1‑141 (MQ=255)
                                                                                         tCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTa                                        <  2:281090/141‑1 (MQ=255)
                                                                                         tCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTa                                        <  2:659944/141‑1 (MQ=255)
                                                                                         tCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCCTATGGATATCATATTCCTTAATATTGATACGTACAACTGGACATGCATTa                                        <  1:573244/141‑1 (MQ=255)
                                                                                         gcTTGAGTCTTTTATCGTGGATCTTCTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCAGAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTa                                        <  2:306867/140‑1 (MQ=255)
                                                                                          ctatggtatataaTCGAGTATCTACTTGTCGATATGTTGGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGTATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTaa                                       >  2:189495/13‑141 (MQ=255)
                                                                                           tttAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTaaa                                      <  2:494893/141‑1 (MQ=255)
                                                                                           tttAGTCTTTTATCGGGTATCTACTTGTCGATATCTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGCGGTCCATATGGATGTCATATTCATTAATATTGATACGTACAACTGGACATGCATTaaa                                      <  1:525136/141‑1 (MQ=255)
                                                                                                tCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTAt                                 >  2:42511/1‑141 (MQ=255)
                                                                                                 cTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTAtt                                >  1:568511/1‑141 (MQ=255)
                                                                                                  ttttATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTa                               >  1:392552/1‑141 (MQ=255)
                                                                                                    ttATCGTGTATTTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAAt                             <  2:371044/141‑1 (MQ=255)
                                                                                                     tATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATc                            <  2:737882/141‑1 (MQ=255)
                                                                                                       tCGTGTATCTATTGGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATcta                          <  2:529552/141‑3 (MQ=255)
                                                                                                        cGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCaa                         >  2:292440/1‑141 (MQ=255)
                                                                                                         gtgtATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAAt                        >  1:407485/1‑141 (MQ=255)
                                                                                                         gtgtATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAAt                        >  1:725429/1‑141 (MQ=255)
                                                                                                         gtgtATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAAt                        >  1:623620/1‑141 (MQ=255)
                                                                                                           gtATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTCATCATAGGAGTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCg                      >  2:311210/1‑141 (MQ=255)
                                                                                                            tATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGt                     <  1:819857/141‑1 (MQ=255)
                                                                                                              tCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCa                   <  2:343683/141‑1 (MQ=255)
                                                                                                              tCTACTTGGCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCa                   <  1:466937/141‑1 (MQ=255)
                                                                                                                  cTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTCTATCATAGGATTTAAAGCTTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAATTGGACATGCATTAAAGCTCTTTATCCAATCGTCATAGc               <  1:483054/141‑1 (MQ=255)
                                                                                                                  cTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTATATCATAGGATTTAAAGTTTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAAATGGACATGCATTAAAGCTTTTCATCCAATCGTCATAGc               <  1:483171/141‑1 (MQ=255)
                                                                                                                  cTTGGCGATATGTTTGAAGAATTCAATGAATTTTTTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTCGACATGCATTAAAGCTATTAATCCAATCGTCATAGc               <  2:675647/141‑1 (MQ=255)
                                                                                                                  cTTCTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGAGATCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGAACATCCATACAAGCTCTTAACCCAAACGTCATacc               >  2:16682/1‑139 (MQ=255)
                                                                                                                       cgcaatGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTGCAAGGTTCGGTGTCCTTATGGATATCATATTCATTAATATTTGTACGTACAACTGGACATGCATTAAAGCTATTAATCCATTCGTCATTGCGGTTa          <  2:362061/137‑1 (MQ=255)
                                                                                                                       cGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTa          <  1:467091/141‑1 (MQ=255)
                                                                                                                          tatGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAAt       <  1:764517/141‑1 (MQ=255)
                                                                                                                          tatGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAAt       <  1:359379/141‑1 (MQ=255)
                                                                                                                           atGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATa      <  2:94828/141‑1 (MQ=255)
                                                                                                                               ttGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCtt  <  1:439048/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                 
GTATATGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCGAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTT  >  CP000730/607746‑608013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: