Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 702,669 T→C 3.7% N154N (AAT→AAC USA300HOU_0650 → possible CPA1 family monovalent cation (K+ or Na+):proton (H+) antiporter‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730702,6690TC 3.7% 341.1 / 2.5 117N154N (AAT→AACUSA300HOU_0650possible CPA1 family monovalent cation (K+ or Na+):proton (H+) antiporter‑1
Reads supporting (aligned to +/- strand):  ref base T (53/58);  new base C (2/3);  total (56/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.25e-01

Only 100 of 126 total aligned reads displayed.

CCAGATTTACCTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTTATAGGTATGGCATT  >  CP000730/702529‑702809
                                                                                                                                            |                                                                                                                                            
ccAGATTTACCTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGCGCAATGACAATTCTTGAAAGTACGTCAGTATTGAat                                                                                                                                              >  1:771949/1‑141 (MQ=255)
ccAGATTTACCTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGGCATTATTGAat                                                                                                                                              >  1:752450/1‑141 (MQ=255)
 cAGATTTACCTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAatg                                                                                                                                             <  1:554491/141‑1 (MQ=255)
 cAGATTTACCTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATGCTTGAAGGTGAGTCATTATTGAatg                                                                                                                                             <  1:725227/141‑1 (MQ=255)
  aGATTTACCTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAatga                                                                                                                                            <  2:196665/141‑1 (MQ=255)
    aTTTACCTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAatgatg                                                                                                                                          <  1:242242/141‑1 (MQ=255)
         ccTATTGGAGTAGCATTTGCAATTGCTGCCATTCTCTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGAtgctgcg                                                                                                                                     >  2:195011/1‑140 (MQ=255)
          cTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGGGAGTCATTATTGAATGATGctggtg                                                                                                                                    >  2:248911/1‑141 (MQ=255)
           tATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTgg                                                                                                                                   <  1:451129/141‑1 (MQ=255)
             taccagcagcATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGta                                                                                                                                 >  2:192995/5‑141 (MQ=255)
             taccagcagcATTTGCAATTGCTGCCATTCTTTGTCCTACTGAAGCAGTAGCAGAGCAAGCAATCACTAAAGGAAAGGCCTTGCAAAAAGGAGCAATGACCATGCGTGAAGGTGAGTCATTATTGAATGATGCTGCTGGta                                                                                                                                 >  2:737462/5‑141 (MQ=255)
             taccagcagcAGTGGCAAGTGGTGCCATTCTGTGTCCTGCTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGAAGTATTGATGAGGCTTAGAGGTGAGTCATTATTGAATGATGCTGCTGGta                                                                                                                                 >  2:829541/5‑141 (MQ=255)
                  gcagcaTTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTattatt                                                                                                                            <  1:805136/141‑1 (MQ=255)
                     gcaTTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCa                                                                                                                         >  1:329239/1‑141 (MQ=255)
                      caTTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCAt                                                                                                                        >  1:655769/1‑141 (MQ=255)
                      caTTTGCAATTGCTGACATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGAAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCAt                                                                                                                        >  2:588931/1‑141 (MQ=255)
                       aTTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCAtt                                                                                                                       >  2:270053/1‑141 (MQ=255)
                           gaaaTTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTAATAATTGAATGATGCTGCTGGTAGTATTTCATTTaaa                                                                                                                   <  1:382363/139‑1 (MQ=255)
                            cAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTaaaa                                                                                                                  <  1:470606/141‑1 (MQ=255)
                            cAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTaaaa                                                                                                                  <  1:462478/141‑1 (MQ=255)
                            cAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTaaaa                                                                                                                  <  2:114818/141‑1 (MQ=255)
                            cAATTGCTGCCATTATCTGTCCTACTGATGCAGTAGCACTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCAGTATTGAATGATGCTGCTGGTATTATTTCATTTaaaa                                                                                                                  >  1:480234/1‑141 (MQ=255)
                               ttGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAg                                                                                                               >  2:496603/1‑141 (MQ=255)
                                  ctgtCATTCTTTGGCCTACTTATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTg                                                                                                            <  1:831792/141‑1 (MQ=255)
                                  ctgcCATTCTTTGTCCTACTGATGCAGTAGCGGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTg                                                                                                            <  2:385863/141‑1 (MQ=255)
                                     ccATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTg                                                                                                         >  2:531896/1‑141 (MQ=255)
                                     ccATTCTTTCTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTg                                                                                                         <  1:115031/141‑1 (MQ=255)
                                     ccATTCGTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGTTTGTATTATTTCATCTATAATAGCTGTTg                                                                                                         <  2:8428/141‑1 (MQ=255)
                                       aTTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGa                                                                                                       <  1:244320/141‑1 (MQ=255)
                                         tCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTGAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGt                                                                                                     <  2:152804/141‑1 (MQ=255)
                                         tCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGt                                                                                                     >  2:494814/1‑141 (MQ=255)
                                              gTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAg                                                                                                >  1:187776/1‑141 (MQ=255)
                                               gccTACTGATGCTGTAGCGGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGTGCAATGACTATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGt                                                                                               <  2:216118/140‑1 (MQ=255)
                                               gccTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGt                                                                                               <  2:864777/140‑1 (MQ=255)
                                                    cTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAg                                                                                          <  1:651193/141‑1 (MQ=255)
                                                       aTGCAGTAGCAGTGCAAGCAATCACTGAAGGAAAGATCTTGCCAAAAGGAGCGATGGCAATTCTTGAAGGTGAGGCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTGACAGGTg                                                                                       >  1:664940/1‑141 (MQ=255)
                                                       aTGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTg                                                                                       >  1:8066/1‑141 (MQ=255)
                                                          cAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTCTTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCtt                                                                                    >  2:627383/1‑141 (MQ=255)
                                                          cAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCtt                                                                                    >  2:678116/1‑141 (MQ=255)
                                                               gCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCa                                                                               >  2:535323/1‑141 (MQ=255)
                                                                 aGTACAATCAATCACTAAACGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCATGACGGTGCGTCATTATTGGATGATGCTGCTTGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACt                                                                             <  2:467483/141‑1 (MQ=255)
                                                                  gTGCAAGCAATCACTCAGAGAGCGCATCTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCCCtt                                                                            >  1:418607/1‑141 (MQ=255)
                                                                       agcaaTCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGa                                                                       >  2:401095/1‑141 (MQ=255)
                                                                        gcaaTCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTTACTTGTTGAt                                                                      <  2:585624/141‑1 (MQ=255)
                                                                          aaTCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCCCTTGTTGATGc                                                                    >  1:372582/1‑141 (MQ=255)
                                                                          aaTCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTCGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCCTTTAAAATAGCTGTTGGAGTATTAGTTCCAGGTGCTTTTTCACTTGTTGATGc                                                                    <  1:427834/141‑1 (MQ=255)
                                                                           aTCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAACGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACttgttgcagcg                                                                   >  2:424366/1‑136 (MQ=255)
                                                                           aTCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAACGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCt                                                                   >  2:790428/1‑141 (MQ=255)
                                                                              gcTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACCGGTGCTTTTTCACTTGTTGATGCTGtt                                                                >  2:482620/2‑141 (MQ=255)
                                                                              aCTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTAACTGTTGAAGGTGCTTTTTCACTTGTTGATGCTGtt                                                                <  1:417357/141‑1 (MQ=255)
                                                                               cTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTc                                                               >  2:254900/1‑141 (MQ=255)
                                                                                   aggaaaggTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAgtt                                                           <  2:631211/141‑1 (MQ=255)
                                                                                       aaggTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTTAGTTGttt                                                       >  2:156172/1‑141 (MQ=255)
                                                                                       aaggTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGttt                                                       <  1:349136/141‑1 (MQ=255)
                                                                                       aaggTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGttt                                                       >  1:59579/1‑141 (MQ=255)
                                                                                        aggTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATCAGTTACAGGTGCTTTTTCCCTTGTTGATGCTGTTCAGTTGtttt                                                      <  1:474485/141‑1 (MQ=255)
                                                                                         ggTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGttttt                                                     >  2:61739/1‑141 (MQ=255)
                                                                                         ggTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGttttt                                                     >  2:503040/1‑141 (MQ=255)
                                                                                         ggTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGttttt                                                     >  1:53653/1‑141 (MQ=255)
                                                                                          gTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTCTGCTATTTTAAAGGAGAGGTGCTTTTTCACTTGATGATGCTGGTCAGTTGGTTTTa                                                    >  1:625842/1‑141 (MQ=255)
                                                                                           tCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTaa                                                   >  1:469430/1‑141 (MQ=255)
                                                                                                  aaaaGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGATGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCGGTTCAGTTGTTTTTGATTGAAtt                                            >  2:238738/1‑140 (MQ=255)
                                                                                                   aaaGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGATGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCa                                           >  2:788036/1‑141 (MQ=255)
                                                                                                      ggAGCAATGACAATTCTTGGAGTTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAGAATAGCTGTTGGAGTATTAGTTACAGGTGGTTTTTCAATTGTTGATGCTGTGCAGTTGTTTTTAATTGCATCAAtt                                        >  2:434441/1‑141 (MQ=255)
                                                                                                       gAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATtg                                       <  1:244747/141‑1 (MQ=255)
                                                                                                         gCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATtggt                                     >  2:46114/1‑141 (MQ=255)
                                                                                                          cAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATtggtg                                    >  2:822498/1‑141 (MQ=255)
                                                                                                           aaTGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTTTTTATGCTGCTCAGTTGTCTTTAATTGCATCAATtggtgg                                   >  2:715204/1‑141 (MQ=255)
                                                                                                           aaTGACAATTCTTGAAGGGGAGTCATTATTGAAAGATGCTGCTGGTATTATTTCATTTAAAATAGCGGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATtggtgg                                   >  1:181185/1‑141 (MQ=255)
                                                                                                             tGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGgcg                                 <  1:61746/141‑1 (MQ=255)
                                                                                                             tGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGgcg                                 <  2:149430/141‑1 (MQ=255)
                                                                                                               aCAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCa                               <  1:640953/141‑1 (MQ=255)
                                                                                                                cgaTTCTTGAAGGTGAGTCATTATTTAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTGTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAg                              <  2:712669/139‑1 (MQ=255)
                                                                                                                cAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTACAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCCCTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAg                              <  1:100710/141‑1 (MQ=255)
                                                                                                                cAATTCTTGAAGGTGAGTCATTATTGAACGATGCTGCTGGTATTATTTCATTTAAACTAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAg                              <  1:168660/141‑1 (MQ=255)
                                                                                                                cAAGTCTTGAAGGTGAGTCATTATGGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTGACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAg                              <  1:161121/141‑1 (MQ=255)
                                                                                                                   ttCTTGAAGGTGAGTCATTATTGAATGGTGCTGCTGGTATTATTTCATTTAAAATAGCTGTGGGAGGATTAGTTACAGGTGCTTTTTCACTTGTGGATGCTGTTCAGTTGTTTTTAATTGCGTCAATTGGTGGCGCAGtgg                           >  2:846918/1‑141 (MQ=255)
                                                                                                                   ttCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtgg                           >  2:343697/1‑141 (MQ=255)
                                                                                                                   ttCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtgg                           <  1:270053/141‑1 (MQ=255)
                                                                                                                   ttCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGATGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAAATGCATCAATTGGTGGCGCAGtgg                           <  2:619026/141‑1 (MQ=255)
                                                                                                                    tcctGAAGGTGAGTCATTCTTGAATGATGCTGATGGTATTATTTCATCTACCATAGCTGTTGGAGTCTTAGTTCCAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtggt                          <  1:829541/138‑1 (MQ=255)
                                                                                                                    tCTTGTAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAGATGGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtggt                          <  1:737462/141‑1 (MQ=255)
                                                                                                                    tCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtggt                          <  1:192995/141‑1 (MQ=255)
                                                                                                                    tCTTGAAGGTGAGTCATTATTGAACGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtggt                          <  1:790428/141‑1 (MQ=255)
                                                                                                                    tCTTGAAGGTGAGTCATTATTGAACGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtggt                          <  1:424366/141‑1 (MQ=255)
                                                                                                                     cTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtggtt                         <  1:494814/141‑1 (MQ=255)
                                                                                                                      tttaaGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAACAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtggttg                        <  1:197796/138‑1 (MQ=255)
                                                                                                                       tGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTGCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGtggttgg                       <  2:580646/141‑1 (MQ=255)
                                                                                                                        gAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTTTTTTTTTTTTGCTGTTTAGTTGTTTTTAATTGCATCCCTTGGTGGCGCTGtgggtggt                      >  2:716443/1‑141 (MQ=255)
                                                                                                                         aaGGTGAGTCATTATTGAATGAAGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTggttggtt                     <  1:581253/141‑1 (MQ=255)
                                                                                                                             ttaggcATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACt                 <  2:370488/136‑1 (MQ=255)
                                                                                                                             tGATTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACt                 <  2:584248/141‑1 (MQ=255)
                                                                                                                             ggAGTCATTATTGAATGATGCCGCTGGTATTATTTCATCTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTGCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCACCAATTGGTGGCGCAGTGGTTGGTTTACt                 <  1:43937/140‑1 (MQ=255)
                                                                                                                                 gcATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTtatt             <  2:147009/140‑2 (MQ=255)
                                                                                                                                  ccttattGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTTATAg            <  1:248911/139‑1 (MQ=255)
                                                                                                                                    ttattGAATGATGGTGCTTGGAGAATGTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTTATAGGt          <  1:323797/141‑1 (MQ=255)
                                                                                                                                        tGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTTATAGGTATgg      >  1:739198/1‑141 (MQ=255)
                                                                                                                                        tGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTTATAGGTATgg      >  1:857767/1‑141 (MQ=255)
                                                                                                                                        tGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTTATAGGTATgg      <  1:478552/141‑1 (MQ=255)
                                                                                                                                            tgatgTTGCTGGTATTATTTTATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGGTTGTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTTATAGGTATGGCAtt  <  1:703136/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CCAGATTTACCTATTGGAGCAGCATTTGCAATTGCTGCCATTCTTTGTCCTACTGATGCAGTAGCAGTGCAAGCAATCACTAAAGGAAAGGTCTTGCCAAAAGGAGCAATGACAATTCTTGAAGGTGAGTCATTATTGAATGATGCTGCTGGTATTATTTCATTTAAAATAGCTGTTGGAGTATTAGTTACAGGTGCTTTTTCACTTGTTGATGCTGTTCAGTTGTTTTTAATTGCATCAATTGGTGGCGCAGTGGTTGGTTTACTTATAGGTATGGCATT  >  CP000730/702529‑702809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: