Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,269,458 G→T 7.7% D173Y (GAT→TAT)  gid → glucose‑inhibited division protein Gid

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,269,4580GT 7.7% 159.1 / 3.0 51D173Y (GAT→TAT) gidglucose‑inhibited division protein Gid
Reads supporting (aligned to +/- strand):  ref base G (27/20);  new base T (2/2);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

CAGATGGATACACAATTATCGCAACAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCGCCTGTAAATTCATTTGA  >  CP000730/1269318‑1269591
                                                                                                                                            |                                                                                                                                     
cAGATGGATACACAATTATCGCAACAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATgg                                                                                                                                       >  2:370617/1‑141 (MQ=255)
     cgctACACAGGTATCGAACCTGGACCACTTCCTATAGAAACCCTTGCGCAAGAAAGAGTGGACATTACTGGTAGAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATaaa                                                                                                                                  <  2:817033/138‑1 (MQ=255)
      gATACACAATTATCGCAACAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAg                                                                                                                                 >  2:813945/1‑141 (MQ=255)
       aTACACAATTATCGCAACAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGt                                                                                                                                <  2:5185/141‑1 (MQ=255)
                       aCAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGt                                                                                                                <  1:169269/141‑1 (MQ=255)
                        cAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGtt                                                                                                               <  1:570672/141‑1 (MQ=255)
                        cAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGtt                                                                                                               >  2:92698/1‑141 (MQ=255)
                        cAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTAGTGATATGGATAAAGTTTACTTAAAGTCCCGtt                                                                                                               <  1:620073/141‑1 (MQ=255)
                             ccACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGAt                                                                                                          >  1:825179/1‑141 (MQ=255)
                              cACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGTATAAAGTTTACTTAAAGTCCCGTTATGATa                                                                                                         >  1:211237/1‑141 (MQ=255)
                              cACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGTATAAAGTTTACTTAAAGTCCCGTTATGATa                                                                                                         >  1:210373/1‑141 (MQ=255)
                                 ttACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAg                                                                                                      >  1:362354/1‑141 (MQ=255)
                                  tactacAGAAACCCTTGCGCAAGAAATCGTGGACATTACTGGTACAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAgg                                                                                                     <  2:442405/141‑1 (MQ=255)
                                    ctacAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTg                                                                                                   >  1:353512/1‑141 (MQ=255)
                                    ctacAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTg                                                                                                   <  1:603047/141‑1 (MQ=255)
                                                ttGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAGGGTGAAGCTGCATAtt                                                                                       >  1:396006/1‑141 (MQ=255)
                                                ttGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATAtt                                                                                       >  2:293556/1‑141 (MQ=255)
                                                 tGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATAttt                                                                                      <  1:237453/141‑1 (MQ=255)
                                                  gcgcAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGCTATGGATAAAGTTTACTTAAAGTCCCTTTATGATAAAGGTGAAGCTGCATATTTa                                                                                     <  2:358283/141‑1 (MQ=255)
                                                      aaGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACt                                                                                 <  1:565890/141‑1 (MQ=255)
                                                       aGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTg                                                                                >  1:69773/1‑141 (MQ=255)
                                                        gAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTACAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAGGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGt                                                                               >  1:290623/1‑141 (MQ=255)
                                                          aaTAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTcc                                                                             <  1:92698/141‑1 (MQ=255)
                                                          aaTAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTcc                                                                             <  2:509629/141‑1 (MQ=255)
                                                                 gACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGGCACCCAACATCTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACt                                                                      >  1:851205/1‑141 (MQ=255)
                                                                  aCATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTg                                                                     >  2:314592/1‑141 (MQ=255)
                                                                  aCATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTg                                                                     <  1:813945/141‑1 (MQ=255)
                                                                  aCATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTg                                                                     >  1:40649/1‑141 (MQ=255)
                                                                  aCATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTg                                                                     >  2:25650/1‑141 (MQ=255)
                                                                       aCTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGTATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGAt                                                                <  2:210373/141‑1 (MQ=255)
                                                                       aCTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGTATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGAt                                                                <  2:211237/141‑1 (MQ=255)
                                                                        cTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATg                                                               >  2:684089/1‑141 (MQ=255)
                                                                         tGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGa                                                              >  1:817155/1‑141 (MQ=255)
                                                                                  tCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCg                                                     >  2:792765/1‑141 (MQ=255)
                                                                                  tCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCg                                                     >  2:195609/1‑141 (MQ=255)
                                                                                  tCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCg                                                     >  2:866281/1‑141 (MQ=255)
                                                                                  tCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCg                                                     <  2:353512/141‑1 (MQ=255)
                                                                                              ttATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGc                                         <  1:592585/140‑1 (MQ=255)
                                                                                              cTATGATGCGGCTGCTCAAATTATTGAAAAAGAATTTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGc                                         <  1:588590/141‑1 (MQ=255)
                                                                                                        gctgctCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGaa                               <  1:300110/141‑1 (MQ=255)
                                                                                                           gctCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCt                            >  1:208543/1‑141 (MQ=255)
                                                                                                           gctCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCAGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGacact                            >  2:644561/1‑137 (MQ=255)
                                                                                                           gctCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGGTATGATAAAGATGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCt                            >  2:307282/1‑141 (MQ=255)
                                                                                                             tCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTAAATTTTAAATCTTTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGa                          >  1:629628/1‑141 (MQ=255)
                                                                                                                    attGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTgcg                   <  2:69773/141‑1 (MQ=255)
                                                                                                                      tGAAAAAGAATCTATTGATATGGCTAAAGTTTACGGTAGGTCCCGTTATGCTCGCGGAGAAGCGGCAGATTTAAACTGTCCTATGACGGAGGATGACTTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCGcc                 >  1:163253/1‑141 (MQ=255)
                                                                                                                         aaaaGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCGCCTGt              >  1:739673/1‑141 (MQ=255)
                                                                                                                           aaGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACGGAGGATCAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCGCCTGTaa            >  2:571562/1‑141 (MQ=255)
                                                                                                                               aTCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCGCCTGTAAATTc        <  1:320223/141‑1 (MQ=255)
                                                                                                                                 cTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCGCCTGTAAATTCAt      >  2:307920/1‑141 (MQ=255)
                                                                                                                                 cTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCGCCTGTAAATTCAt      >  2:269969/1‑141 (MQ=255)
                                                                                                                                     tGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTAAAAAAAAAAAATAACTGAGGAAGAATTAAATCGCTTTTATGATGCAGTATAAGAAGCTGAAGTTGCGCCTGTAAAttaaaaaaa  <  2:48138/141‑8 (MQ=255)
                                                                                                                                            |                                                                                                                                     
CAGATGGATACACAATTATCGCAACAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCGGCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGCATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCGCCTGTAAATTCATTTGA  >  CP000730/1269318‑1269591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑