Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,857,867	C→T	10.0%	L169L (TTG→TTA)	USA300HOU_1728 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,857,867	0	C	T	10.0%	151.2 / 4.0	64	L169L (TTG→TTA)	USA300HOU_1728	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/27);  new base T (2/5);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAATCTCTTGTGC  >  CP000730/1857727‑1858007                                                                                                                                             |                                                                                                                                             tcttcttTTATCGCTGCTTGTTGTGCTTTCACTGCCACTGCTTTTGGTTGTTCATTTGATACAGCTGCACTTTTCTCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTc                                                                                                                                              <  1:78147/141‑1 (MQ=255)  tttcttTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTTTTCATTTTTTACTGCTGCACTTTTGTCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCa                                                                                                                                             <  2:677811/140‑1 (MQ=17)        ttacccctcctTTTTGTTCTTTTAATGCTACTTCTTTTGGTTTTTCATTTGTTACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAattat                                                                                                                                       <  2:235411/132‑1 (MQ=17)          aTCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTAttt                                                                                                                                     >  1:681155/1‑141 (MQ=255)            cGCTGCTTGTTGTGCCTTCAATGCCACTGCTTTTGTTTCTTCTTTTGATACAGCTGCACTTTTATCCGTTTCTGCTTGTGCTTCTTTTTTATCTTCTTGAATCTCTTGTGCTTCTAGTAACGTATCACTCAATTTATTTGc                                                                                                                                   >  2:861323/1‑141 (MQ=14)             gctgctTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCGTTTTTAGCTTCTTGAATATATTGTGCGTCTTGTGATGTATCATTTGCATTATTTGcc                                                                                                                                  >  2:684951/1‑140 (MQ=14)                    gttgtGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCCCTTGTGATGTCTCGCTGAAAATATTTGTACTTGctt                                                                                                                           >  2:262422/1‑141 (MQ=17)                     tttttctgttattGCCACTGTTTTTGGTTTTTCATTTTTTACTGCTGTATTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttc                                                                                                                          <  2:821818/129‑1 (MQ=17)                     tttttcTTTCACTGCTACTGCTTTTGGTTCTTCATTTGTTACAGCTGCACTGTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttc                                                                                                                          <  1:11600/136‑1 (MQ=17)                     ttgtGCTTTCACTTCCACTTCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttc                                                                                                                          <  1:12166/141‑1 (MQ=255)                      tgttCTTTTACTTCCCCTTCTGTTGCTTTTTCATTTGTTGCTGCTGCCCTTTTATCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttct                                                                                                                         <  2:51908/141‑1 (MQ=14)                      tgttCTTTCAATGCCACTTCTTTTGCTTCTTCATTTGTTACTGCTGCACCTTTATCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttct                                                                                                                         <  2:42989/141‑1 (MQ=17)                      tgtgCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCTTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttct                                                                                                                         >  2:369281/1‑141 (MQ=255)                         tttttCTATTCCCCTGCTTTTCGTTCTTTATTTGTTGTTGCTGTACTTTTTTCTGCCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCTTCTTGTGATGTATCACTTAAATTATTTGCACTTGcttcttct                                                                                                                      <  2:300791/139‑1 (MQ=11)                         gCTTTCAATGCCACTGCTTTTGGCTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTTCTTGTGCTTCTTTTTTAGCTTCTTGACTCGCTTGTGCCTCTTGTGATGGATCACCAACATTATTTGCACTTGcttctgct                                                                                                                      >  1:143273/1‑141 (MQ=17)                                  tccACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCgct                                                                                                             <  1:497994/140‑1 (MQ=255)                                    caccgcttcttcaccttcATCTGAAACACCTGCATCTCTATCTGTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCgctgc                                                                                                           <  2:729832/127‑1 (MQ=14)                                    cACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCgctgc                                                                                                           <  1:238087/141‑1 (MQ=255)                                        gCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCttgt                                                                                                       >  1:330103/1‑141 (MQ=255)                                        ccTTTTGGTTCTTCATTTGATACAGCTTCACCTTTATCTTTCTCTGCTTGGGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCGCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCttgt                                                                                                       <  1:604097/140‑1 (MQ=17)                                              ggTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATCTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCt                                                                                                 >  1:297926/1‑141 (MQ=255)                                               ggtcttcATTTGTTACTGCTGTATCTTTATCTGTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCtt                                                                                                <  1:469861/139‑1 (MQ=14)                                                  cttcATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCa                                                                                             >  1:683015/1‑141 (MQ=255)                                                   ttcATTTGATACAGCTGCACTTTTATCTGTTGCTGCTGGTGCTTCTTTTGTAGCTTCTTGAATCTGTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCaa                                                                                            >  1:341477/1‑141 (MQ=255)                                                       tttGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGcc                                                                                        >  1:354707/1‑141 (MQ=255)                                                           aTACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTg                                                                                    >  1:449219/1‑141 (MQ=255)                                                              cAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtt                                                                                 >  2:334513/1‑141 (MQ=255)                                                                gCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                                               >  1:767077/1‑141 (MQ=255)                                                                  tgtctttctATCTGTCTCTTCTTGTGCTTCTTTGTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGa                                                                             <  2:239452/133‑1 (MQ=11)                                                                    cACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGTCACTGCTTTTGAtt                                                                           <  1:638287/141‑1 (MQ=255)                                                                     ctttctttCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTCTTTTGTCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTc                                                                          <  2:562497/134‑1 (MQ=11)                                                                       ttttGTCTTTTGCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTCTCACTTAAATTATTTTCACGTGCTTCTTCTTATATCGCTGTTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCtt                                                                        <  2:142646/141‑1 (MQ=11)                                                                        tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCttt                                                                       >  1:117154/1‑141 (MQ=255)                                                                          tttCTTTTTCTGCTTGTGATTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTAt                                                                     <  1:334087/139‑1 (MQ=255)                                                                                  tCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCCTTCCATGCCACTGCTTTTGATTCTTTATTTGATACa                                                             <  2:341477/141‑1 (MQ=255)                                                                                    tGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCCTTCAATGCCACTGCTTTTGATTCAATATTTTATACacc                                                           >  2:280895/1‑139 (MQ=255)                                                                                    tGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCCTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGc                                                           <  1:544601/141‑1 (MQ=255)                                                                                              tcttTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTa                                                 <  1:411967/141‑1 (MQ=255)                                                                                                ttttttAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACAGCTTTATc                                               <  1:539427/141‑1 (MQ=255)                                                                                                ttttttAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATc                                               >  1:697639/1‑141 (MQ=255)                                                                                                 ttgttAGCTTCGTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATcc                                              <  1:220253/138‑1 (MQ=255)                                                                                                  ttttAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCACAAAGAAGCTTGTTCAATGCCCCTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCg                                             >  1:198420/1‑141 (MQ=17)                                                                                                     tAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCtt                                          >  1:675659/1‑141 (MQ=255)                                                                                                      aGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGcc                                                                                                 >  1:143725/1‑84 (MQ=39)                                                                                                      aGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTc                                         >  1:343441/1‑141 (MQ=255)                                                                                                       gCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCgctgct                                                                                                          <  1:337713/75‑1 (MQ=255)                                                                                                        cttcttGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAat                                                                                                                                          >  1:208232/1‑42 (MQ=255)                                                                                                        cttcttGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTg                                       >  2:493669/1‑141 (MQ=255)                                                                                                              gAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGTTTCTACTTTTATCGCTGCTTGTTGTGCTTTCACTGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCACTTCTGCTtgtg                                 <  1:640027/141‑1 (MQ=255)                                                                                                                 tctcTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTGGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCtt                              >  1:392862/1‑141 (MQ=255)                                                                                                                 cctctTGTGCCTCTTGTGATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCtt                              >  1:348876/2‑141 (MQ=255)                                                                                                                   tctTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTtct                            >  1:29423/1‑141 (MQ=255)                                                                                                                       gtgCCTTGTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACCCTTTTATCCGCTTCTGCTTGTGCTTCTCttt                        <  2:579843/141‑1 (MQ=255)                                                                                                                       gtgCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTTAATGCCACTGCTTTTGTTTCTTTATTTTAGACAGCCACACTGTCATCCGGTTCTACtgttgattttgttt                        >  2:175065/1‑128 (MQ=14)                                                                                                                        tgCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTtgtg                                                                                                   <  1:702066/65‑1 (MQ=255)                                                                                                                         tccTCTTGTGATCTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTa                      <  2:337650/140‑1 (MQ=255)                                                                                                                          ccTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCCCACTTTTATCCGCTTCTGCTTGTACTTCTCTTTTAg                     >  2:484959/1‑141 (MQ=255)                                                                                                                              ttGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGcttt                 >  1:44354/1‑140 (MQ=255)                                                                                                                              ttGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGcttc                 >  1:223203/1‑141 (MQ=255)                                                                                                                              ttGGGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCAGCCACTGCGTGTGCTTCTCTTTTAGcttc                 <  1:418574/141‑1 (MQ=17)                                                                                                                                gtgATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTGGcttctt               >  1:182937/1‑141 (MQ=255)                                                                                                                                gtgATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGcttctt               <  1:262422/141‑1 (MQ=255)                                                                                                                                 tgATATATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTg              <  2:809915/141‑1 (MQ=255)                                                                                                                                  gatatATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGa             <  2:348876/137‑1 (MQ=255)                                                                                                                                     gTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCGCTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTGTTGagtt          >  2:434064/1‑138 (MQ=255)                                                                                                                                             cAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAATCTCTTGTGc  <  1:725067/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                             TCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAATCTCTTGTGC  >  CP000730/1857727‑1858007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑