Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,010,3630CT100.0% 258.2 / ‑6.5 81C146Y (TGT→TAT) USA300HOU_1861ABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (43/37);  total (43/38)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGTAT  >  CP000730/2010225‑2010500
                                                                                                                                          |                                                                                                                                         
cTTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATaa                                                                                                                                         >  1:750412/1‑141 (MQ=255)
 tttCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAAt                                                                                                                                        <  2:612292/141‑1 (MQ=255)
 tttCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGGACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAAt                                                                                                                                        <  2:423125/141‑1 (MQ=255)
          tCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCaaa                                                                                                                               >  1:329109/1‑141 (MQ=255)
             aTTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAatat                                                                                                                            >  1:539892/1‑141 (MQ=255)
                     aaTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAc                                                                                                                    <  1:580731/141‑1 (MQ=255)
                     aaTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAc                                                                                                                    <  2:249341/141‑1 (MQ=255)
                     aaTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAc                                                                                                                    <  1:465656/141‑1 (MQ=255)
                     aaTCTGCCAAACAAAACGTACAACGTTAAAATGTAAAATGGGAATATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAc                                                                                                                    <  2:669977/141‑1 (MQ=255)
                      aTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGGTAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAcc                                                                                                                   <  2:182538/141‑1 (MQ=255)
                      aTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAcc                                                                                                                   <  1:688521/141‑1 (MQ=255)
                       tcgtCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAccc                                                                                                                  <  1:192707/138‑1 (MQ=255)
                          tCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGt                                                                                                               <  2:631075/141‑1 (MQ=255)
                          gccAAAGAAAGCTTACAACGTGAAAATGTAAAATGGGAAAATACAAACAGATGCTAAAGTCAACTCTTTATCTAAATAGAACATGATGTATAATACAATAATAACTGTACTATAAAAGAAGCAAATATTCATTAACCCGGt                                                                                                               <  2:100442/140‑1 (MQ=255)
                             aaaGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTaa                                                                                                            >  2:434021/1‑141 (MQ=255)
                             aaaGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTaa                                                                                                            >  1:173278/1‑141 (MQ=255)
                                gAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAAt                                                                                                         >  2:360114/1‑141 (MQ=255)
                                  aaaCGTGCAGCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATGTCACAGCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTAATTAACCCGGTTAAAATGa                                                                                                       >  2:70961/1‑141 (MQ=255)
                                    aCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGaaa                                                                                                     >  2:222035/1‑141 (MQ=255)
                                    aCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGaaa                                                                                                     >  2:285859/1‑141 (MQ=255)
                                     cGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAATCCAATATTCATTAACCCGGTTAAAATGAAAt                                                                                                    <  2:184253/141‑1 (MQ=255)
                                      gTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATc                                                                                                   >  1:235962/1‑141 (MQ=255)
                                      gTACACCGTTAAAATGGAAAAGGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAAtt                                                                                                   >  1:443384/1‑140 (MQ=255)
                                                  aatgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTc                                                                                       <  2:734378/141‑1 (MQ=255)
                                                         actgGGAAGAACAACAGTGCTGCTAACGTCAATTTGACATCTAAAAATACCCGTATGGATACCGAAATAATAATAGATATATAATCTAACCAAATACTCATTCACCCGGTTAAAATGAAATCTTTtgtttcttgcacctcc                                                                                >  2:240649/3‑133 (MQ=255)
                                                         aatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatca                                                                                <  2:746344/141‑1 (MQ=255)
                                                           tgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTACCCCGGTTAAAGTGAAATCTTTTGTTTGTTCAACATCAtt                                                                              >  1:234899/1‑141 (MQ=255)
                                                              gAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAAt                                                                           <  2:364440/141‑1 (MQ=255)
                                                               aaGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATATTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATc                                                                          <  2:122448/141‑1 (MQ=255)
                                                               aaGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCACTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATc                                                                          <  1:333244/141‑1 (MQ=255)
                                                                 gATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCAc                                                                        >  2:248213/1‑141 (MQ=255)
                                                                 gATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATAATTAATCaa                                                                        >  2:213726/1‑140 (MQ=255)
                                                                  aTAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCATCATCATTAATCAct                                                                       >  1:42171/1‑141 (MQ=255)
                                                                  aTAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGCTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCAct                                                                       >  1:124539/1‑141 (MQ=255)
                                                                    aaaCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCActct                                                                     <  2:329109/141‑1 (MQ=255)
                                                                      accgTGCTTCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTAGATAATCTAACCAAATATTCATTAACCCGGTTAAAATCAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAg                                                                   <  2:645983/138‑1 (MQ=255)
                                                                      aCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAg                                                                   <  2:72949/141‑1 (MQ=255)
                                                                             tgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTAc                                                            <  2:731886/141‑1 (MQ=255)
                                                                             tgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTAc                                                            <  2:534450/141‑1 (MQ=255)
                                                                              gctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTCTATAATCTAACCAAATATTCATTAACCTGGTTCAAATGAAATCTTTTGTTTGTTCAACATACTTAATCACTCTAGATAtttat                                                           >  1:93102/1‑138 (MQ=255)
                                                                               ctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACtt                                                          >  1:378078/1‑141 (MQ=255)
                                                                                tAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTg                                                         >  2:573359/1‑141 (MQ=255)
                                                                                  aaGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGAc                                                       <  2:495597/141‑1 (MQ=255)
                                                                                       aaTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACt                                                  <  2:643071/141‑1 (MQ=255)
                                                                                       aaTTTCACATCTAAAAAGAACATTATGAATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACt                                                  <  1:407610/141‑1 (MQ=255)
                                                                                        aTTTCACATCTAAAAAGAACATTATGTATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACtt                                                 <  2:656678/141‑1 (MQ=255)
                                                                                        aTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACtt                                                 <  2:619589/141‑1 (MQ=255)
                                                                                             acaTCTGAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACGTACTTGatta                                            >  2:221155/1‑141 (MQ=255)
                                                                                              caTCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATCTTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTATTTgtgtct                                           >  2:55237/1‑136 (MQ=255)
                                                                                              caTCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTGCTTGattct                                           >  1:439252/1‑139 (MQ=255)
                                                                                                 cTAAAAAGAACATTATGGCTAGTGAAAGAATAATTGTGATATAATATAACAAAATATTAATTAATCCGGTTAAAGGGAAAATTTTTGGTTATTCAACATCTTCACTAATGTTAGATATTACTTGACCTACTTGattattcg                                        >  2:369127/1‑139 (MQ=255)
                                                                                                   aaaaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGCTTATTAGCa                                      >  2:82384/1‑141 (MQ=255)
                                                                                                   aaaaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCa                                      <  2:480387/141‑1 (MQ=255)
                                                                                                   aaaaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCa                                      <  1:867244/141‑1 (MQ=255)
                                                                                                   aaaaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCa                                      >  1:488895/1‑141 (MQ=255)
                                                                                                   aaaaaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTACTTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCa                                      >  1:240507/1‑141 (MQ=255)
                                                                                                      aaGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTTGCATaa                                   >  2:304530/1‑141 (MQ=255)
                                                                                                          aCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtc                               <  2:419406/141‑1 (MQ=255)
                                                                                                          aCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtc                               <  2:35910/141‑1 (MQ=255)
                                                                                                           cATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCGAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTTAACATCATTAATCACTCTAGCTATTACTTGACCTACTTGATTATTAGCATAAAAtct                              >  2:35245/1‑141 (MQ=255)
                                                                                                           cATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGCTTATTAGCATAAAAtct                              >  1:571837/1‑141 (MQ=255)
                                                                                                             ttATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCg                            >  2:307233/1‑141 (MQ=255)
                                                                                                              tATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGc                           <  1:794944/141‑1 (MQ=255)
                                                                                                                tGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCAc                         <  2:338237/141‑1 (MQ=255)
                                                                                                                    tAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTaa                     >  1:806969/1‑141 (MQ=255)
                                                                                                                      gTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTCAAATGCACTCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATAATTAGCCTAAAATCTCGCACttctgt                   >  1:528036/1‑137 (MQ=255)
                                                                                                                      gTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAg                   >  2:110928/1‑141 (MQ=255)
                                                                                                                      gTGCAATAATAATTGGTATATAATCTAACCAAGTATTCATTAACCCGGTTAAAATCACATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTGGCATAAAATCTCGCACTTAAAg                   >  1:672876/1‑141 (MQ=255)
                                                                                                                       tGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGc                  >  2:399408/1‑141 (MQ=255)
                                                                                                                        taaataataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAACCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCt                 <  2:93102/139‑1 (MQ=255)
                                                                                                                        gCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATATCGCACTTAAAGCt                 >  1:321370/1‑141 (MQ=255)
                                                                                                                         cAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt                >  1:44059/1‑141 (MQ=255)
                                                                                                                         cAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCtt                >  2:719383/1‑141 (MQ=255)
                                                                                                                          aataataatTGTTATATAATCTAACCAAATATTCAATAACCCGGTTAAAATGAAATCTGTTGTTTGTTCAACATCATTAATCTCTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTg               <  2:539892/141‑1 (MQ=255)
                                                                                                                             aataatTGTTATATAATCTAACCAGATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTaa            <  2:412493/141‑1 (MQ=255)
                                                                                                                             aataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAAACCTTTTAGAGATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTGAAAGCTTGTaa            >  1:851519/1‑141 (MQ=255)
                                                                                                                               taatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAAt          >  1:346485/1‑141 (MQ=255)
                                                                                                                                aatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTAACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTaatgt         >  2:295744/1‑138 (MQ=255)
                                                                                                                                    tttATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAACTCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTg     <  2:443384/140‑1 (MQ=255)
                                                                                                                                     ttATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTGGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGt    >  1:675453/1‑141 (MQ=255)
                                                                                                                                     tattaCAATCTAACCAAATCTTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGCCCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGt    <  1:228544/138‑1 (MQ=255)
                                                                                                                                       atatAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGtat  <  1:285859/141‑1 (MQ=255)
                                                                                                                                       atatAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGtat  <  1:222035/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CTTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTGTAT  >  CP000730/2010225‑2010500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: