Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,468,697 C→T 9.7% E39K (GAA→AAA)  USA300HOU_2331 ← possible esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,468,6970CT 9.7% 248.9 / 5.1 91E39K (GAA→AAA) USA300HOU_2331possible esterase
Reads supporting (aligned to +/- strand):  ref base C (41/40);  new base T (8/2);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 1.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCACCGTGAATATATAAAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCTTAACCTACCTTTATA  >  CP000730/2468557‑2468831
                                                                                                                                            |                                                                                                                                      
tCCACCGTGAATATATAAAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTc                                                                                                                                        <  1:380194/141‑1 (MQ=255)
 ccACCGTGAATATATAAAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTTAAGGCTGCTTATGTTTTTTTCTATTTTCa                                                                                                                                       >  2:113641/1‑141 (MQ=255)
 ccACCGTGAATATATAAAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTACTATTTTCa                                                                                                                                       >  2:760644/1‑141 (MQ=255)
  cACCGTGAATATATAAAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATGATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCaa                                                                                                                                      <  2:645486/141‑1 (MQ=255)
   aCCGTGAATATATAGAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAAt                                                                                                                                     <  1:741651/141‑1 (MQ=255)
   aCCGTGAATATATAAAATTTTCTTATCAATTTGATTTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAAt                                                                                                                                     <  2:145735/141‑1 (MQ=255)
     cGTCAATATATTAAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAACGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAAttt                                                                                                                                   <  1:322818/141‑1 (MQ=255)
          atatagAAGATTTTTCTTTCGATTTGATGTCGGACATTAAAGCGAGAGACTGGCATATCAGCTAAGGACAATTTTTCTAAATTTGCTTCAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCtt                                                                                                                              <  2:515146/141‑1 (MQ=255)
           tatataAAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCttt                                                                                                                             >  1:845765/1‑141 (MQ=255)
                aaaaTTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATaa                                                                                                                        >  1:759104/1‑141 (MQ=255)
                       tCTTATCAATTTGATGTCTGAAATTAAAGAGAAACAACATTATACGATGTTGTTATAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGATGCTTATGTTTTTTTCTTTTTTTAATTTAAATTATAAAAAAAAATc                                                                                                                 <  2:829516/141‑1 (MQ=255)
                         ttATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATaaaaaattttt                                                                                                               >  1:621801/1‑136 (MQ=255)
                         ttATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCtt                                                                                                               >  1:680776/1‑141 (MQ=255)
                          tATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTTTATTTTTTTTTTCTCTTTTATAATAAAATCttt                                                                                                              >  2:239730/1‑141 (MQ=255)
                          tATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAACATCttt                                                                                                              >  2:251944/1‑141 (MQ=255)
                          tATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCttt                                                                                                              >  1:325494/1‑141 (MQ=255)
                           aacgaTTGGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTc                                                                                                             >  2:655887/5‑141 (MQ=255)
                           aTCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTTTTTTTTTTTTTTCTCTTTTATAAAAAAATCtttt                                                                                                             >  2:596455/1‑140 (MQ=255)
                           aTCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTc                                                                                                             >  2:598865/1‑141 (MQ=255)
                            tCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCa                                                                                                            >  1:789206/1‑141 (MQ=255)
                            tCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCa                                                                                                            >  1:789422/1‑141 (MQ=255)
                              aaTTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAAc                                                                                                          >  1:580571/1‑141 (MQ=255)
                                  tGATGTCTGAAATTACAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACAtat                                                                                                      >  1:433853/1‑139 (MQ=255)
                                   gATGTCTGAAATTAAAGCGAAGGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCTACATCtt                                                                                                     >  1:261903/1‑141 (MQ=255)
                                     tGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGTAGGCTGCTTATGTTTTTTTCTATTTTCGATTTCTATTTTATTAGTAAATCTTCCACCTTTTTGa                                                                                                   >  2:862420/1‑141 (MQ=255)
                                     tGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGa                                                                                                   <  1:821169/141‑1 (MQ=255)
                                     agaatGAAATTAAGGCGAAAGACTGGCATATAATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTAAACGCTGCTCGTCTTTTTTTTTCTCTTCAATTTTTCTTTTATTAAAAAATCTTTAACCATGTTGa                                                                                                   >  2:237184/5‑141 (MQ=255)
                                      atCTGAAAATAAAGAGAAAGACTTGCATATCATCTAATGAAAATTTTTCTAAACTTGCTTTAACATTTAAGGTTAAATGCTGCTTATGTTTTTTTCTATTTTCAATTTAAAAAAAAAAAAAAAATCTTTCAACATCTTGAt                                                                                                  <  2:742352/140‑1 (MQ=255)
                                       tCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATTACAATTTTTCTACATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATc                                                                                                 <  1:542260/141‑1 (MQ=255)
                                       tCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATc                                                                                                 <  1:210324/141‑1 (MQ=255)
                                       tCTGAAATTAAAGCGAAAGACTTGCATATCATCGAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATc                                                                                                 <  1:587610/141‑1 (MQ=255)
                                        cTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTTTCAAAAAATCTTTCCACATCTTgtgct                                                                                                >  1:386600/1‑137 (MQ=255)
                                          gAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCAtt                                                                                              >  1:757267/1‑141 (MQ=255)
                                            aaTTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCAtttt                                                                                            <  2:702726/141‑1 (MQ=255)
                                              ttAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTa                                                                                          <  1:322965/141‑1 (MQ=255)
                                               tAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTCTAAAAACATATTTCTACCTCTTGCTCATATTTat                                                                                         >  1:56023/1‑140 (MQ=255)
                                               tAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATCTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTaa                                                                                         >  1:25257/1‑141 (MQ=255)
                                                aaaGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTaaa                                                                                        >  1:71636/1‑141 (MQ=255)
                                                  aactaaaGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACa                                                                                      >  2:655865/5‑141 (MQ=255)
                                                  aGCGAATTACTTGTATATCATCTAATGACAATTTTTATAAATCTGCTTTAACATTTAATGTTGAAGGCTGTTTATGTTTTTTTCTATTTTCAATTAAAAAAAAAAAAAAAAATTTTGCAACATCTTGATCATTTTTAAACa                                                                                      <  2:185371/141‑1 (MQ=255)
                                                  aGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACa                                                                                      <  2:272828/141‑1 (MQ=255)
                                                  aGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTATTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACa                                                                                      >  1:805615/1‑141 (MQ=255)
                                                   gCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACAt                                                                                     >  1:767467/1‑141 (MQ=255)
                                                    cGAAAGACTTGCATATCATCTAATGATAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATa                                                                                    <  2:245985/141‑1 (MQ=255)
                                                    cGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATa                                                                                    >  1:204444/1‑141 (MQ=255)
                                                    cGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATa                                                                                    >  2:498551/1‑141 (MQ=255)
                                                           cTTGCATATCATCTTATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTCTAAACATAATCGAGc                                                                             <  1:433160/141‑1 (MQ=255)
                                                                   tcatcTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGa                                                                     <  1:623543/141‑1 (MQ=255)
                                                                    catcTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGaa                                                                    <  1:740622/141‑1 (MQ=255)
                                                                    catcTAATGACAATTTTGCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGaa                                                                    <  2:273566/141‑1 (MQ=255)
                                                                            gACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAatat                                                            >  2:867501/1‑141 (MQ=255)
                                                                              cAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTTTTTTTTTTTTTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAAtattt                                                          >  2:838694/1‑141 (MQ=255)
                                                                              cAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTTAACATCCTGAACATAAGTAAGCATAATCGAGCGATTGTGAAGCAAAtattt                                                          >  1:370801/1‑141 (MQ=255)
                                                                               caTTTTTCTAAATATGCTTTATCATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTCTATAAAAAACTCTTTCAACATTCTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAAtattta                                                         <  1:395298/140‑1 (MQ=255)
                                                                               aaTTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTTTTTTTTTTATTTCTCTTTTATGAAAAAATCTTTTAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAAtattta                                                         >  2:623321/1‑141 (MQ=255)
                                                                               aaTTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAAtattta                                                         >  2:9838/1‑141 (MQ=255)
                                                                               aaTTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAAtattta                                                         <  1:4383/141‑1 (MQ=255)
                                                                                aTTTTCCTAAATTTTCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAAtatttat                                                        <  1:295427/141‑1 (MQ=255)
                                                                                              gCTTTAACATTTAATGTTGAAGGCTGCTTAAGTTTTTTTCTATTTTCAATTTCTCTTTTATACAAAAATCTTTCAACATCTTGCTCATTTTTAACAATACTCGCGCGATTGTGAAGCAAATGTTTATTGACAACACTATTc                                          >  2:314279/1‑141 (MQ=255)
                                                                                                 ttAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTCAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATa                                       <  1:455030/141‑1 (MQ=255)
                                                                                                 ttAACATTTAATGTTGAAGGCGTCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAACAACATCTTTCAACATCTTGATCAGTTTTAAACATCATCGAGCGATTGGGGAGTCAATATTTATTGTCAACGCTATTCATa                                       <  1:452069/141‑1 (MQ=255)
                                                                                                  tAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAACAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGAAAATATTTATTGACAACGCTATTCATaa                                      <  1:557712/141‑1 (MQ=255)
                                                                                                  tAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATaa                                      <  2:386198/141‑1 (MQ=255)
                                                                                                     taTTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAacac                                   <  2:576096/140‑1 (MQ=255)
                                                                                                     cctttAATGTTGAAGGCTGCTTATGTTTTGTTCTCTTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCACTTTTAAACATAATCGAGCGATTGTGAAGCACATATTTATTGACAACGCTATTCATAacac                                   <  2:466574/139‑1 (MQ=255)
                                                                                                        ttAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGt                                >  1:790769/1‑141 (MQ=255)
                                                                                                        ttAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGt                                >  1:354187/1‑141 (MQ=255)
                                                                                                        ttAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGt                                >  1:316818/1‑141 (MQ=255)
                                                                                                         tAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGtt                               <  1:739494/141‑1 (MQ=255)
                                                                                                          aaTGTTGAAGGCTGCTTATGTTTTTTTTTTTTTTTTTTTTCTCTTTTATAAAAAAATCTTTTAACTTCTTGATCGTTTTTAAACATAATCGACCTCCTGTGATGTAACTATTTATTGTCAACGCTGTTCATaatttggttt                              >  2:440027/1‑133 (MQ=255)
                                                                                                           aTGTTGAAGGCTGCTTATGTTTTTTTTTTTTTTTTTTTTCTCTTATATAAAAAAATCTTTCAACGTCTTGATCATTTTTAAACATAATCGAGCGCTTGTGAAGCAAAAATTTATTGACAACGCTATTCATAACACGGtttt                             >  2:835368/1‑140 (MQ=255)
                                                                                                           aTGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATAATTTTTAAACATAATCGAGCGATTGTGAAGCAAATACGGATTGCCAACGCTATTCATCACACGGtttt                             >  2:45485/1‑140 (MQ=255)
                                                                                                             gTTGAAGGCTGCTTATGTTTTTTTTTTTTTTTTTTTTCTCTATTATAAAAAAATCTTTCAACTTCTTGATCTTTTTTAAACATAATCGAGCGATCGTGAAGCACATATTTTTTGACAACGCTATTCATTACATTGTTTTTa                           >  2:577458/1‑141 (MQ=255)
                                                                                                              ttGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTaa                          <  1:63407/141‑1 (MQ=255)
                                                                                                               tGAAGGCTGCTTATGTTTTTTTCTATTTTTAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAAt                         <  1:234803/141‑1 (MQ=255)
                                                                                                               tGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAAt                         <  1:651029/141‑1 (MQ=255)
                                                                                                                 aaGGCTGTTTATGTTTTTTTCTATTTTCAATTTCACTTTTATAAAAAAATCTTTCATCATCTTGATCATTTTTAAACATAATCGAGCGACCGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCa                       <  2:372927/141‑1 (MQ=255)
                                                                                                                 aaGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCa                       <  1:760644/141‑1 (MQ=255)
                                                                                                                   ggCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAAt                     >  2:376766/1‑141 (MQ=255)
                                                                                                                     ctgctTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTTTTTTTTTTTTTTCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGt                   >  2:791050/1‑141 (MQ=255)
                                                                                                                       gctTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGCGCGATTGGCAAAAAAATCTCCAGCGACAACGCTATTCATAACACGGTTCCTAATCAATGTCt                 >  2:829761/1‑141 (MQ=255)
                                                                                                                       gctTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCt                 >  1:41024/1‑141 (MQ=255)
                                                                                                                       gctTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCt                 <  2:462536/141‑1 (MQ=255)
                                                                                                                       gctTATGTTTTTCTCTATTTTCAATTTCTCTTTGATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCt                 <  2:484349/141‑1 (MQ=255)
                                                                                                                       gctCATGTCTTTTTCTATTTTCAATATCTCTTTTATAAAACAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGCTTGTGAAGCAAATATTTCTTTACAACGCTATTCATAACACGGTTTCTACTCAATGtat                 >  2:562094/1‑139 (MQ=255)
                                                                                                                        ctTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCtt                <  2:759104/141‑1 (MQ=255)
                                                                                                                         ttATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGTCAACGCTATTCATAACACGGTGTCTAATCAATGTCTTa               <  1:481931/141‑1 (MQ=255)
                                                                                                                            tgtgTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCCTGCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCTTAacc            <  2:391399/141‑1 (MQ=255)
                                                                                                                            tGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCTTAacc            <  1:546836/141‑1 (MQ=255)
                                                                                                                                    tCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCTTAACCTACCTTta    <  2:69153/141‑1 (MQ=255)
                                                                                                                                      tATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCTTAACCTACCTTtatt  >  1:356141/1‑140 (MQ=255)
                                                                                                                                      tATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCTTAACCTACCTTtata  >  1:307224/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                      
TCCACCGTGAATATATAAAATTTTCTTATCAATTTGATGTCTGAAATTAAAGCGAAAGACTTGCATATCATCTAATGACAATTTTTCTAAATTTGCTTTAACATTTAATGTTGAAGGCTGCTTATGTTTTTTTCTATTTTCAATTTCTCTTTTATAAAAAAATCTTTCAACATCTTGATCATTTTTAAACATAATCGAGCGATTGTGAAGCAAATATTTATTGACAACGCTATTCATAACACGGTTTCTAATCAATGTCTTAACCTACCTTTATA  >  CP000730/2468557‑2468831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑