Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,604,421 C→T 6.5% H430Y (CAT→TAT)  USA300HOU_2464 → AbgT family p‑aminobenzoyl‑glutamate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,604,4210CT 6.5% 239.3 / 3.6 84H430Y (CAT→TAT) USA300HOU_2464AbgT family p‑aminobenzoyl‑glutamate transporter
Reads supporting (aligned to +/- strand):  ref base C (48/30);  new base T (3/3);  total (51/33)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TAGAACATCAAAACGGTATTGTATTGATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCTTACTTT  >  CP000730/2604281‑2604559
                                                                                                                                            |                                                                                                                                          
taGAACATCAAAACGGTATTGTATTGATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGAAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCtctc                                                                                                                                            >  2:670811/1‑141 (MQ=255)
    aCATCAAAACGGTATTGTATTGATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATcc                                                                                                                                        <  1:345649/141‑1 (MQ=255)
        cAAAACGGTATTGTATTGATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAGTCTTAATCATTAAGGGATTTCATccaaaa                                                                                                                                    >  2:714404/1‑137 (MQ=255)
        cAAAACGGTATTGTATTGATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCa                                                                                                                                    <  1:85692/141‑1 (MQ=255)
           aaCGGTATTGTATTGATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTCCATCCCGCATTc                                                                                                                                 >  2:201124/1‑141 (MQ=255)
                tattgtattgATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTcacaca                                                                                                                            >  1:510872/1‑141 (MQ=255)
                 attgtattgATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCGCACaa                                                                                                                           >  1:172244/1‑141 (MQ=255)
                 attgtattgATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACaa                                                                                                                           >  1:717651/1‑141 (MQ=255)
                  ttgtattgATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACCCAAg                                                                                                                          >  1:16608/1‑141 (MQ=255)
                   tgtattgATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGt                                                                                                                         >  2:20976/1‑141 (MQ=255)
                    gtattgATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTc                                                                                                                        >  1:605557/1‑141 (MQ=255)
                      attgATAATAGGTATTATTGAACTGAGTGCAATGGTCAACATGATAATCGGAAGCACATCGCCTAAATGGGGTATTTAAGGACCGATATTCGTAACAATGTTAATCCTTATCGGCTTTCATACCGCATTCACACaaaccat                                                                                                                      >  2:274967/1‑136 (MQ=255)
                        tgATACTAGGTATTATTGTACTGAGGTCAATTGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTACCGGCTTTCATCCCGCATTCACACAAGTCAttt                                                                                                                    >  2:478804/1‑141 (MQ=255)
                         taTACTAGGTATTTTTGTACCGAGTGCAATGGTCAACATGTGTATCGGAAGCGCATCGGCTAAAGTGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTa                                                                                                                   <  2:164394/140‑1 (MQ=255)
                           tACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTAcc                                                                                                                 <  2:536720/141‑1 (MQ=255)
                             cTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCgt                                                                                                               >  1:223370/1‑141 (MQ=255)
                             cTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCgt                                                                                                               >  2:860937/1‑141 (MQ=255)
                                 gTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAg                                                                                                           <  1:695877/141‑1 (MQ=255)
                                  tattatTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCGCGCATTCACACAAGTCATTTACCGTGTAgg                                                                                                          >  1:265979/1‑141 (MQ=255)
                                       ttGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTCGGACCGATATTCGTACCAATGTTGATCCTTATCGGCTTTCATCCCGCATTCCCACCCGTCATTTACCGTGTGGGCGGtt                                                                                                     >  1:216902/1‑141 (MQ=255)
                                       ttGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTCTAGGCGAtt                                                                                                     >  2:386549/1‑141 (MQ=255)
                                         gTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCa                                                                                                   <  1:732331/141‑1 (MQ=255)
                                          tACTGAGTGCAATGGTCAACAATTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCaa                                                                                                  <  1:839095/141‑1 (MQ=255)
                                                  gCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTAccaat                                                                                          >  1:700526/1‑141 (MQ=255)
                                                   cAATGGTCAACATGTTAATCGGAAGCGCATCGGCTCAATGGGGTATTTTAGGACCGATATTCGTGCCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCCATTCccaatc                                                                                         >  2:6456/1‑141 (MQ=255)
                                                   cAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTAccaatc                                                                                         >  1:353775/1‑141 (MQ=255)
                                                   cAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTAccaatc                                                                                         >  1:635408/1‑141 (MQ=255)
                                                            aCATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTacac                                                                                >  2:824907/1‑141 (MQ=255)
                                                             cATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACAcc                                                                               >  2:383525/1‑141 (MQ=255)
                                                               tGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCga                                                                             <  2:707051/141‑1 (MQ=255)
                                                                 ttAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCgatg                                                                           >  1:507785/1‑141 (MQ=255)
                                                                 ttAAGCGGAAGCGCACCGGATAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCgatg                                                                           <  2:160983/141‑1 (MQ=255)
                                                                   aaTCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCgatgat                                                                         >  1:849530/1‑141 (MQ=255)
                                                                   aaTCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCgatgat                                                                         >  2:789555/1‑141 (MQ=255)
                                                                    aTCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGCTTATAAAACCTATGTTAATCCTTATCGGCTTTTATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGatgatg                                                                        >  1:817143/1‑141 (MQ=255)
                                                                    aTCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTTATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCgatgatc                                                                        >  1:13545/1‑140 (MQ=255)
                                                                    aTCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTTATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGatgatg                                                                        >  1:566345/1‑141 (MQ=255)
                                                                     tCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGc                                                                       <  1:708463/141‑1 (MQ=255)
                                                                      cGGAGGCGCATCGGCTAGATGGGGTATCTGAGTGCCGATATTCGTGCCAATTTTAATCCTTATCGGCTCTCATCCCGCATTAACACAAGTCAGTTACCGTGTAGGCGATTCAACCACCAATCCAATTACACCGATGATGcc                                                                      <  2:694906/141‑1 (MQ=255)
                                                                      cGGACGCGCATCGGCTAAAGGGTGTATTGTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGcc                                                                      <  2:42798/141‑1 (MQ=255)
                                                                      cGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGcc                                                                      >  1:177581/1‑141 (MQ=255)
                                                                         aaGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCACCCCAATTACACCGATGATGCCGTa                                                                   <  1:860937/141‑1 (MQ=255)
                                                                         aaGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTa                                                                   >  2:326826/1‑141 (MQ=255)
                                                                            cgcATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTGAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACtt                                                                <  1:585504/141‑1 (MQ=255)
                                                                                tCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCt                                                            <  2:510872/141‑1 (MQ=255)
                                                                                 tagCTAAATTGGTTATTTGAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCtt                                                           <  2:866401/139‑1 (MQ=255)
                                                                                 cGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCtt                                                           >  1:491558/1‑141 (MQ=255)
                                                                                 cGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCtt                                                           <  2:380008/141‑1 (MQ=255)
                                                                                 cGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAAACCAATTACACCGATGATGCCGTACTTACAtt                                                           >  2:500182/1‑141 (MQ=255)
                                                                                      aaaTGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTttatta                                                      >  1:575249/1‑141 (MQ=255)
                                                                                       aattgtgTATTTTAGCGTCGATATTCGTACCAATGTTATTTCGTTTCGGCTTTCCTCCCGCATTCACACAAGTCATGTACCGTGTAGGCGTTTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTttattat                                                     <  2:442036/135‑1 (MQ=255)
                                                                                         tGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTttattatta                                                   >  2:784093/1‑141 (MQ=255)
                                                                                            ggTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTGACACAAGTCATTTACCGTGTAGGCGGTTCAATTACCAATCCAATTACACCGATGATGCCGTCCTTACCTTTATTATTAACt                                                >  2:218318/1‑141 (MQ=255)
                                                                                            ggTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACt                                                >  2:147278/1‑141 (MQ=255)
                                                                                                  ttAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGca                                          <  1:749004/141‑1 (MQ=255)
                                                                                                      gACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACAAAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACAACGATGATGCCGTACTTACCTTTATTATTAACTTTTGCACaaa                                      >  1:506/1‑141 (MQ=255)
                                                                                                       aCCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACaaaa                                     >  1:634887/1‑141 (MQ=255)
                                                                                                         cGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCACTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAAt                                   <  2:270455/141‑1 (MQ=255)
                                                                                                         cGATATTCGTACCAATGTTAATCCTTATCGGCTTGCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGCTTCAATTACCAATCCAATTCCACCGATGACTCCGCACTTACCTTCGTTATTAACGTACGcccacacac                                   >  2:110486/1‑136 (MQ=255)
                                                                                                             atTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATCCGa                               >  2:114774/1‑141 (MQ=255)
                                                                                                               tCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCTTTCATTTGGCACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATa                             >  1:628972/1‑141 (MQ=255)
                                                                                                               tCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATa                             >  2:222542/1‑141 (MQ=255)
                                                                                                                cGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTCCCGTGTAGGCGATTCAATTACCAATCCGATTGCACTGATGATGCCGTACTTACCTTTATTATTAACTTATGCGCAAAGATACGATaa                            >  2:817875/1‑141 (MQ=255)
                                                                                                                cGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATaa                            >  2:413587/1‑141 (MQ=255)
                                                                                                                 gTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATaaa                           <  1:20976/141‑1 (MQ=255)
                                                                                                                        tGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTCTTATTAACTTATGCACAAACATACGATAAACGCATGa                    >  1:233078/1‑141 (MQ=255)
                                                                                                                        tGTTAATCCTTATCGGCTTCCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGa                    <  1:201124/141‑1 (MQ=255)
                                                                                                                           taacacTTATCGGCTTTTATCCCGCATTCACACAAGTCATGTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAAc                 <  2:817143/136‑1 (MQ=255)
                                                                                                                           tAATCCTTATCGGCTTTTATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACGTACCTTTATTATTAAATAAAAAAAAAAAATACGATAAACGCATGAAAc                 <  2:13545/141‑1 (MQ=255)
                                                                                                                           tAATCCTTATCGGCTTTTATCCCGCATTCACACAAGTCATTTACCGTGTACGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAAc                 <  2:566345/141‑1 (MQ=255)
                                                                                                                             aTCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATAAGATAAACGCCTGAAACTc               >  2:630802/1‑141 (MQ=255)
                                                                                                                                catATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGa            >  2:533492/3‑141 (MQ=255)
                                                                                                                                  tATCGGCTTTCCTCCCGCATTCACACAAGTCATTTACCGTGTATGCGATTCAATTTCCTATCCAAATACACCGAAGCTGTCGTACTTACTTTTATTATTAACTTATGCACAAAAATACGACATACGCATGCAACTCGGAGc          <  2:506/141‑1 (MQ=255)
                                                                                                                                   ttCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTCCCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGcc         <  2:34699/140‑1 (MQ=255)
                                                                                                                                   aTCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGcc         >  2:4157/1‑141 (MQ=255)
                                                                                                                                   aTCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGcc         >  2:775674/1‑141 (MQ=255)
                                                                                                                                    tCGGCTTTCATCCCGCATTCACACTAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTTCCTTTATTATTAACTTATGCACAAAAATACTATAAAAACATGAAACACGGAGCCt        >  2:133846/1‑141 (MQ=255)
                                                                                                                                    tCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCt        <  2:177581/141‑1 (MQ=255)
                                                                                                                                      ggCTTGCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCTTa      <  1:170113/141‑1 (MQ=255)
                                                                                                                                       gCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCTTAc     <  1:344477/141‑1 (MQ=255)
                                                                                                                                         tttCATCCCGCATTCACACTAGTCGTTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATCCACGCATGAAACTCGGAGCCTTACtt   <  1:122474/141‑1 (MQ=255)
                                                                                                                                          ttCATCCCGCATTCACACACGTCCTGTCCCGTGTAGGCGATTCAATTACCAATCCAAGTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCTTACttt  <  2:128586/141‑1 (MQ=255)
                                                                                                                                          ttCATCCCGCATTCACACAAGTCCTTTGTCGTGTAGGCGATTCAATTACCATTCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCTTACttt  <  2:787046/141‑1 (MQ=255)
                                                                                                                                          ttCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCACTCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCTTACttt  >  2:612964/1‑141 (MQ=255)
                                                                                                                                          ttCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGAAGCCTTACttt  >  1:709773/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TAGAACATCAAAACGGTATTGTATTGATACTAGGTATTATTGTACTGAGTGCAATGGTCAACATGTTAATCGGAAGCGCATCGGCTAAATGGGGTATTTTAGGACCGATATTCGTACCAATGTTAATCCTTATCGGCTTTCATCCCGCATTCACACAAGTCATTTACCGTGTAGGCGATTCAATTACCAATCCAATTACACCGATGATGCCGTACTTACCTTTATTATTAACTTATGCACAAAAATACGATAAACGCATGAAACTCGGAGCCTTACTTT  >  CP000730/2604281‑2604559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑