Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,679,725 G→A 100% W70* (TGG→TAG)  USA300HOU_2536 → possible secretory antigen

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,679,7250GA100.0% 234.0 / ‑6.5 79W70* (TGG→TAG) USA300HOU_2536possible secretory antigen
Reads supporting (aligned to +/- strand):  ref base G (0/2);  major base A (35/39);  minor base C (2/1);  total (37/42)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAA  >  CP000730/2679589‑2679858
                                                                                                                                        |                                                                                                                                     
cAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTAt                                                                                                                                   <  2:691133/141‑1 (MQ=255)
 aaTCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCCATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTAtt                                                                                                                                  <  1:497399/141‑1 (MQ=255)
   tCCGTCGAACAAACGCATCAATCGCACGCCCCAGCTAAAAATCATAGTCAAAAACAATGTCACCGCAGGGCCACTCAACGGAGCGCACAAGTCTTTAAACCCGTATCTAATAGATGGGGCAACGCTAAAAATTAGTATTAc                                                                                                                                >  2:856186/1‑141 (MQ=255)
     cGTCGAACAAACGCCTCAATCGCATGCCGCAGTTCATTATTAAAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCTCAAGTCGGTAAACATGTTACTAATAGCTAGGGCATGGCTAAAACTTCGTATTATaa                                                                                                                              >  2:176222/1‑141 (MQ=255)
     cGTCGAACAAACGCATCAATCGCATGCCGCAGTTCATTATTATAGTAAAAACCAATGTACATGGTGGGCCTTTAAACTTCGCGCACCAGACGGTAAACCTGTTTCTAATAGATGGGACAATGCTAGAAATTAGTATTACaa                                                                                                                              >  2:123107/1‑141 (MQ=255)
     cGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACaa                                                                                                                              >  1:683528/1‑141 (MQ=255)
       tCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGAATTTCAATg                                                                                                                            >  2:60347/1‑141 (MQ=255)
            cAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTAGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGt                                                                                                                       <  2:22556/141‑1 (MQ=255)
            aaaaaGCAGCAGTCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGt                                                                                                                       <  2:779495/140‑1 (MQ=255)
               aCGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTaaa                                                                                                                    <  1:627920/141‑1 (MQ=255)
               aCGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTaaa                                                                                                                    <  1:627865/141‑1 (MQ=255)
                 ccATCAATCGCATGCCTCAGTTAATTATTATAGTAAAAACCAATTTACATGGGGTGCATTTAAACGTCGCGCACAATTCGTTAAACCTTTTTCTAATACATGTGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATc                                                                                                                  <  2:66719/140‑1 (MQ=255)
                   ctCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCaa                                                                                                                <  2:331977/140‑1 (MQ=255)
                   aTCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCaa                                                                                                                <  1:544846/141‑1 (MQ=255)
                   aTCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCaa                                                                                                                <  1:23994/141‑1 (MQ=255)
                        tCGCATGGCGCAGTTAATGATTAAAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATTGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAatat                                                                                                           <  1:227717/141‑1 (MQ=255)
                          gCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTGTTACAATGAACGTAAATCAAAATATGc                                                                                                         >  1:253759/1‑141 (MQ=255)
                          gCATGCCGCAGTTAATGAGTAGAGTAAAAACGACAGTACATGGGGGGCATTTAAAAGTGGGGCCCAAGTCCGGAATGATGTATCCAGTAGATGGGGCAAGACTAGAAATTAGTATTACAATGCACGTAAAGCAAAATATgg                                                                                                         >  2:20650/1‑140 (MQ=255)
                              tccGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTTGGCAGTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGAGGCAATGCTAAAAATTGGTATTACTATGCACGTAAATCAAAATATGCGACt                                                                                                     <  2:284967/140‑1 (MQ=255)
                               ccGCAGTTAATTATTATAGTAAAAACCAAAGTACATGGTGGGCATTTAAACGACGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATACGACTg                                                                                                    >  2:681411/1‑141 (MQ=255)
                                cGCAGTTAATTATTATAATAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTgg                                                                                                   <  2:347488/141‑1 (MQ=255)
                                  cAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTTAATCAAAATATGCGACTGGTc                                                                                                 <  1:449508/141‑1 (MQ=255)
                                  cAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTc                                                                                                 <  1:93666/141‑1 (MQ=255)
                                   aGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCg                                                                                                <  2:730022/141‑1 (MQ=255)
                                         ttattaTAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTCGTATTACAATGGACGTAAATCAAAATATGCGACTGGTCGTACAcc                                                                                          >  1:585026/1‑141 (MQ=255)
                                           attatAGCCACAACCAATGTACCGGGTGGGCAGTTAAACGTCGCGGACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCaa                                                                                        <  2:19596/141‑1 (MQ=255)
                                             tataGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGa                                                                                      >  1:388732/1‑141 (MQ=255)
                                                aGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGaaaa                                                                                   <  2:453974/141‑1 (MQ=255)
                                                aGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACAAAGaaaa                                                                                   >  2:20098/1‑141 (MQ=255)
                                                    aaaaCCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTg                                                                               <  2:600786/141‑1 (MQ=255)
                                                          aaTGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCa                                                                         <  2:683528/141‑1 (MQ=255)
                                                          aaTGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCa                                                                         >  1:800588/1‑141 (MQ=255)
                                                           aTGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCAt                                                                        >  1:300276/1‑141 (MQ=255)
                                                           aTGTACATGGTGGGCATTTAAACGTCGCGAAAAAGTCAGTAAACCTATTTCTAATAGATGGGGCAATGCTAAAAATTAGTCTTTTTGTGTATAAATTTTAAAATATTCGACTGGTCGTACACCAAGAAAATTTGATGTcca                                                                        >  1:239917/1‑139 (MQ=255)
                                                              tACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTACACCTGTTCCTAGTAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCa                                                                     <  1:280709/141‑1 (MQ=255)
                                                                  tggtggGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCa                                                                 >  1:602562/1‑141 (MQ=255)
                                                                   ggttgGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCaa                                                                <  1:191170/141‑1 (MQ=255)
                                                                        gCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCa                                                           <  1:211200/141‑1 (MQ=255)
                                                                        gCATCTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCa                                                           >  1:588054/1‑141 (MQ=255)
                                                                         cATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAg                                                          <  1:509871/141‑1 (MQ=255)
                                                                            ttAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACAGCCGGat                                                       >  2:764788/1‑141 (MQ=255)
                                                                               aaCGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCGGGagagt                                                    >  1:154331/1‑137 (MQ=255)
                                                                                aCGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGAtatta                                                   <  2:552593/141‑1 (MQ=255)
                                                                                  gTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGAAATCAACTGCAGAATCATATg                                                 >  2:99870/1‑141 (MQ=255)
                                                                                    cgcgCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCACTGCGCTTAATGAGTACAACCATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCGGTGCTGCACTCACCTGCAGCACATTATGGa                                               >  2:745997/1‑141 (MQ=255)
                                                                                       gcACGAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTAATACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACag                                            >  2:526693/1‑140 (MQ=255)
                                                                                       gcACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACAt                                            >  2:567181/1‑141 (MQ=255)
                                                                                         acaAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGt                                          <  1:532832/141‑1 (MQ=255)
                                                                                         acaAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGt                                          <  1:749757/141‑1 (MQ=255)
                                                                                         acaAGTCGGTAAACCTGTTTCTAATAGAGGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCACGATATTATGGACATGt                                          <  1:430550/141‑1 (MQ=255)
                                                                                          caAGTCGGTAAACCTGTTCCTAAGAGATGGGGCAATGCTAAAAATTAGCATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTc                                         <  2:99452/141‑1 (MQ=255)
                                                                                            aGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGc                                       <  1:221825/141‑1 (MQ=255)
                                                                                               cGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAgt                                    >  1:37432/1‑141 (MQ=255)
                                                                                               cGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGAAATTATGGACATGTCGCatt                                    >  2:442873/1‑139 (MQ=255)
                                                                                                gggaacccTGTTTCTAATAGATGGGGCAATGCTAAAACATACAACTGTAAACCACGTAAATCAAAATATGCGACTGGGCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAgtt                                   <  1:280289/135‑1 (MQ=255)
                                                                                                 gTATACCTGTTTCTAATAGAGGGGGTAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAATTATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGGAATCAACTGCAGGATATTATGGACATGTCGCAgttg                                  <  1:681411/141‑1 (MQ=255)
                                                                                                 gTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAgttg                                  <  1:45057/141‑1 (MQ=255)
                                                                                                  tAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAACTTAGCAATCATGCAATCAACTGCAGGAAATAATGAACATGTAGGAgtatt                                 >  2:635732/1‑138 (MQ=255)
                                                                                                      ccTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGaa                             >  1:694261/1‑141 (MQ=255)
                                                                                                      ccTGTTTCTAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGGTGTTGaa                             >  2:597525/1‑141 (MQ=255)
                                                                                                              tAATAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGtata                     <  1:711247/141‑1 (MQ=255)
                                                                                                               aaTAGATGGTGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATTCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGtatat                    <  2:417638/141‑1 (MQ=255)
                                                                                                               aaTAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGtatat                    <  2:417582/141‑1 (MQ=255)
                                                                                                                aTAGATGGGGCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTCCTGTCATGCAATCCACTGCAGGATATTATGGAACTCTCCCAGTTGTTGCACAAGtatata                   >  2:46913/1‑141 (MQ=255)
                                                                                                                      ggggCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGAAATTATGGACATGTCGCAATTGTTGAAAAAGTAAATaaaaaag             >  2:805792/1‑139 (MQ=255)
                                                                                                                       gggCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACgg            >  1:296348/1‑141 (MQ=255)
                                                                                                                       gggCAATGCTAAAAACTAGTATTACGATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACgg            >  1:432694/1‑141 (MQ=255)
                                                                                                                        gggaaagCTAAAAATTAGGATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgt           <  1:98358/135‑1 (MQ=255)
                                                                                                                        ggCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCGTGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgt           >  1:282613/1‑141 (MQ=255)
                                                                                                                        ggCAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgt           >  2:550782/1‑141 (MQ=255)
                                                                                                                        ggCAAAGCTAACAATTCGCATTATAATGCACGTAAACCAAAATCTGCGTCTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAGAAACGgt           <  1:178915/141‑1 (MQ=255)
                                                                                                                          cAATGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgtag         >  1:446471/1‑141 (MQ=255)
                                                                                                                           aaTGCTAAACATTAGTATTACAATGCACGTAAATCAACATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGgtagt        <  1:20650/141‑1 (MQ=255)
                                                                                                                             tGCTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTAt      <  2:388732/141‑1 (MQ=255)
                                                                                                                               cTAAAAATTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTa    <  2:275743/141‑1 (MQ=255)
                                                                                                                                aaaaaaTTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAACAAAATTTGCTGGCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTaa   <  2:55760/140‑1 (MQ=255)
                                                                                                                                 aaaaaTTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTaaa  >  1:98463/1‑141 (MQ=255)
                                                                                                                                 aaaaaTTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTaaa  >  2:371291/1‑141 (MQ=255)
                                                                                                                                 aaaaaTTAGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTaaa  >  1:98384/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                     
CAATCCGTCGAACAAACGCATCAATCGCATGCCGCAGTTAATTATTATAGTAAAAACCAATGTACATGGTGGGCATTTAAACGTCGCGCACAAGTCGGTAAACCTGTTTCTAATAGATGGGGCAATGCTAAAAATTGGTATTACAATGCACGTAAATCAAAATATGCGACTGGTCGTACACCAAGAAAATTTGCTGTCATGCAATCAACTGCAGGATATTATGGACATGTCGCAGTTGTTGAACAAGTATATAAAAACGGTAGTATTAAA  >  CP000730/2679589‑2679858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: