Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,850,161 A→G 4.2% intergenic (+150/‑2) USA300HOU_2691 → / → pcp rhodanese domain sulfurtransferase/C15 family pyroglutamyl‑peptidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,850,1610AG 4.2% 345.1 / 2.8 113intergenic (+150/‑2)USA300HOU_2691/pcprhodanese domain sulfurtransferase/C15 family pyroglutamyl‑peptidase I
Reads supporting (aligned to +/- strand):  ref base A (56/51);  new base G (3/2);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

AATACCTTTCAAAACACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCTTTTAAGAA  >  CP000730/2850021‑2850299
                                                                                                                                            |                                                                                                                                          
aaTACCTTTCAAAACACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTaa                                                                                                                                            >  2:776497/1‑141 (MQ=255)
   aCCTTTCAAAACACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATCAAATTTTAATACTGCGGGCTGTCTTAAAAt                                                                                                                                         >  1:154591/1‑141 (MQ=255)
    ccTTTCAAAACACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAAta                                                                                                                                        >  1:399839/1‑140 (MQ=255)
    ccTTTCAAAACACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATg                                                                                                                                        <  1:358866/141‑1 (MQ=255)
    ccTTTCAAAACACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTATTTTTTATGCGCATACATAACGTGATATGTTTTTAATTCTGCGCGTGTTAGACTTAATAAAATTTTTATAGTGGGGGGGGTCTTAAAATg                                                                                                                                        >  2:625704/1‑141 (MQ=255)
        tCAAAACACGCTTTGAAAATCGGATTTATAAAGGTTATTCAATGTTTTTTTTTATGCTCATACATAACCGGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTTCGGGGTGTTATAAACTGaaca                                                                                                                                    >  2:812798/1‑141 (MQ=255)
        tCAAAACACGCTTTGAAAATCCGTTTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGcaca                                                                                                                                    <  2:53217/141‑1 (MQ=255)
        tCAAAACACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGcaca                                                                                                                                    <  2:640970/141‑1 (MQ=255)
        tCAAAACACGCTTTGAAAATCCAAGTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGcaca                                                                                                                                    <  1:478449/141‑1 (MQ=255)
          aaaaCACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAGAATGCACAtt                                                                                                                                  >  2:156793/1‑141 (MQ=255)
           aaaCACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTTAAATGCACAttt                                                                                                                                 >  1:662671/1‑141 (MQ=255)
           aaaCACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACAttt                                                                                                                                 >  1:235844/1‑141 (MQ=255)
            aaCAGGCATTTAAAAGCCGTTTCATTAATGTTTTTCAAGGCTTTTTTTTACGCGCATACATAACATGATCAGCTTTTAATTATGCTCGTTATATACTAAATTAAATTTTAATATAGCGGGGTGTCTTAAAAAGCTCAtttt                                                                                                                                <  2:41734/141‑1 (MQ=255)
              cacGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTTCGGGGTGTCTTAAAATGCACATTTTAg                                                                                                                              >  2:856703/1‑141 (MQ=255)
              cacGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAg                                                                                                                              >  1:604723/1‑141 (MQ=255)
               acGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTTCGGGGTGTCTTAAAATGCACATTTTAGt                                                                                                                             >  1:510186/1‑141 (MQ=255)
                 ccTTTGAAAATCCCATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATCCACAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTaa                                                                                                                           <  2:830317/140‑1 (MQ=255)
                        aaaTCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGtt                                                                                                                    >  1:676243/1‑141 (MQ=255)
                         aaTCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGttt                                                                                                                   <  1:770299/141‑1 (MQ=255)
                         aaTCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTGTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGttt                                                                                                                   <  2:722484/141‑1 (MQ=255)
                           tCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATCTGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTgc                                                                                                                 <  1:484464/141‑1 (MQ=255)
                               aTTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCt                                                                                                             >  2:318840/1‑141 (MQ=255)
                                tttATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCtt                                                                                                            <  2:596754/141‑1 (MQ=255)
                                       aGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACaa                                                                                                     <  1:553755/141‑1 (MQ=255)
                                       aGGTTTTTCAAGGCTGTTTCTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAAGTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACaa                                                                                                     <  1:686762/141‑1 (MQ=255)
                                        ggTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTg                                                                                                                  <  2:490166/127‑1 (MQ=255)
                                        ggTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGCTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTg                                                                                                                  >  1:490166/1‑127 (MQ=255)
                                          tttttCAAGGGTGTTTTTTATCCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCa                                                                                                  <  1:128417/141‑1 (MQ=255)
                                          tttttCAAGGCTGTTCTCTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTTACAATCa                                                                                                  <  1:539077/141‑1 (MQ=255)
                                           ttttCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCaa                                                                                                 <  2:406221/141‑1 (MQ=255)
                                             ttCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCaaaa                                                                                               >  1:295093/1‑141 (MQ=255)
                                             ttCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCaaaa                                                                                               >  2:316337/1‑141 (MQ=255)
                                              tCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAat                                                                                              <  1:316337/141‑1 (MQ=255)
                                               cAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAAAAGGGTTTGCGCCTTTTGAAAATCaatgta                                                                                             >  2:166344/1‑137 (MQ=255)
                                                 aGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATc                                                                                           <  2:555179/141‑1 (MQ=255)
                                                  ggCTGTTTTTTATGCGCATACATAACCTGATTGGTTTTTAATTATACGCGTGTTATACAAAATTACATTTTAATACTGCGGGGGTACTTAGAATGCACATTTTAGTAACGGGGTTTGCGCACTTTGACAATTaaacaattc                                                                                          >  2:522692/1‑135 (MQ=255)
                                                  ggCTGTTTTTTATGCGCATACATAACCTGATATGTATTTAATTATGCGCGTGTTATACTACATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTCACAGGGTTTGCGCCTTTTGACGATGAAAATCTCa                                                                                          >  2:697835/1‑141 (MQ=255)
                                                         ttttATGCGCATACATCACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTc                                                                                   >  1:430654/1‑141 (MQ=255)
                                                            tATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGGTTGCGCCTTTTGACAATCAAAATATATATCCCTCATg                                                                                >  1:216168/1‑141 (MQ=255)
                                                            tATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATAATAAATTAAATTTTAATACTACGGGGTGTCTTAAAATGCACATGTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCCCATg                                                                                >  2:350861/1‑141 (MQ=255)
                                                             aTGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAGAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATgg                                                                               <  1:156793/141‑1 (MQ=255)
                                                             aTGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAGAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATgg                                                                               <  1:52640/141‑1 (MQ=255)
                                                              tGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATggg                                                                              <  1:834594/141‑1 (MQ=255)
                                                              tGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATggg                                                                              <  1:312768/141‑1 (MQ=255)
                                                                   atacataACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCt                                                                         <  1:681670/141‑1 (MQ=255)
                                                                    tacataACCTTATATGTTTTTAATGAAGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCtg                                                                        <  2:226475/141‑1 (MQ=255)
                                                                    tacataACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCtg                                                                        <  2:510186/141‑1 (MQ=255)
                                                                      cataACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATGCAAAGATCTGTCCGTCATGGGGACCtgtg                                                                      >  1:405443/1‑141 (MQ=255)
                                                                       ataACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAGTACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGa                                                                     >  2:550980/1‑141 (MQ=255)
                                                                       ataACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGa                                                                     >  1:786327/1‑141 (MQ=255)
                                                                       ataACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGa                                                                     >  1:788449/1‑141 (MQ=255)
                                                                        taACCTGATATGTTTTTAATTATGCGCGTGTTATACTAGATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGGGAc                                                                    >  2:5624/1‑141 (MQ=255)
                                                                         aaCCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACt                                                                   <  1:813989/141‑1 (MQ=255)
                                                                          aCCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTc                                                                  <  2:681200/141‑1 (MQ=255)
                                                                             tGATATGTTTTTAATTATGGGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAAc                                                               <  2:450019/141‑1 (MQ=255)
                                                                                tattctgttAATTATGCGCGTGTTAAACTAAATTAAATTTTAATACTGCTGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTCTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTag                                                            <  2:365590/134‑1 (MQ=255)
                                                                                tatGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTag                                                            >  1:75313/1‑141 (MQ=255)
                                                                                  tGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTagaa                                                          <  1:3457/141‑1 (MQ=255)
                                                                                    tttttAATTATGCGGGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACGTTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTagcaga                                                        >  1:462775/1‑141 (MQ=255)
                                                                                     ttttAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGat                                                       >  2:45677/1‑141 (MQ=255)
                                                                                         aaTTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGAtatta                                                   <  1:776497/141‑1 (MQ=255)
                                                                                             aTGCGCGTGTTATAGTAAATTAAAGTTGAATACTGCGGGGTGTCTTAAAAGGCAGATTGTAGTAACAGGATTTGCGCCTTTTGACAATCAAAGTATCAATCCCTCATGTGAAGCTCGGCCTCAACTCTAAGaaatgcaggg                                               >  2:294712/1‑135 (MQ=255)
                                                                                             aTGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTgg                                               >  2:30842/1‑141 (MQ=255)
                                                                                             aTGCGCGTGTTATACTAAATTAAATTCTAATTCTGCGGGGCGGCTTAAAATGCACATTTTAGGAACGGGGTTGGCGCCTTTGAAGAATCAAAATATTAATCCCCCAAGGTAGCCAGTGTCTCAACTATCAGATATTATTgc                                               >  2:294715/1‑140 (MQ=255)
                                                                                              tGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTTATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGc                                              >  2:210526/1‑141 (MQ=255)
                                                                                              tGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGACGCTGTGCCTCAACTCGAAGCTATTATTGGc                                              >  1:820579/1‑141 (MQ=255)
                                                                                                 gcgTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGcaca                                           <  2:113857/141‑1 (MQ=255)
                                                                                                  cgTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGcacac                                          >  1:337463/1‑141 (MQ=255)
                                                                                                   gtgtTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGcacaca                                         <  1:360141/141‑1 (MQ=255)
                                                                                                    tgtTATACTAAATTAAATTTTGATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACAt                                        >  1:445043/1‑141 (MQ=255)
                                                                                                      ttATACTCAATTAAATTGTAATAAAGCGGGGTGTCTTAAAATGCACATTTTAGTAACCGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCGCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCGCACATaa                                      >  1:77035/1‑140 (MQ=255)
                                                                                                      ttATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATAc                                      <  1:210526/141‑1 (MQ=255)
                                                                                                        ataCTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACaa                                    >  1:275584/1‑141 (MQ=255)
                                                                                                         taCTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAAt                                   <  1:131132/141‑1 (MQ=255)
                                                                                                          aCTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATATTAGTAACAAAGATTAAACCTTTTGACAATCAAAATCTCAATCCCTCATGGAGAGCCGAGACCTAACTATAAATAATTATTTGCAGAAATACAATc                                  >  2:818093/1‑141 (MQ=255)
                                                                                                           cTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCg                                 >  2:577896/1‑141 (MQ=255)
                                                                                                                ttaagttTTAAGAATACAGGCAGTCGTAAAAGGCACCTTTTAGTAACTGGAATTCCGCCTTTTGACAACCAAAATATCAACTGCTCATGGGAAGCTGTGACTCAACTAGAAGATATGATTGGCACAAATACGATCGATaaa                            <  2:278881/141‑1 (MQ=255)
                                                                                                                ttaaattTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATaaa                            <  2:278876/141‑1 (MQ=255)
                                                                                                                  aaattTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAAtt                          >  1:717930/1‑141 (MQ=255)
                                                                                                                   aattTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTa                         >  1:819866/1‑141 (MQ=255)
                                                                                                                    attTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACCTACAATCGATACATTaa                        >  1:223781/1‑141 (MQ=255)
                                                                                                                      tttAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTaaaa                      >  2:825573/1‑141 (MQ=255)
                                                                                                                           tACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTGAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTAcc                 >  2:19579/1‑141 (MQ=255)
                                                                                                                           tACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTAcc                 >  1:861595/1‑141 (MQ=255)
                                                                                                                            aCTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATCTTATTGGCACACATACAATCGATAAATTAAAACTACCa                >  2:465567/1‑141 (MQ=255)
                                                                                                                             cTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGGCTCAACTACAAGATATTATTGGCACACATGCACTCGATAAATTAAAACTACCac               >  2:684724/1‑140 (MQ=255)
                                                                                                                             cTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCaa               >  1:149276/1‑141 (MQ=255)
                                                                                                                             cTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCaa               <  1:700235/141‑1 (MQ=255)
                                                                                                                              tGCGGGGTGTCTTAAAATGCTCATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAAc              <  2:20915/141‑1 (MQ=255)
                                                                                                                                  gggTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAGAATATCAATCCCTCATGGGAAGCTGTGACTCAACTACAAGATATTATTGGGACACATACAATCGATAAATTAAAACTACCAACatct          >  2:566578/1‑141 (MQ=255)
                                                                                                                                   ggTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCtt         >  2:497632/1‑141 (MQ=255)
                                                                                                                                     tgtCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCATTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCtttt       <  2:515379/141‑1 (MQ=255)
                                                                                                                                       tCTTATATTGGAAATTTTAGTAACAGGGTTTGCGCCTTTTGACAAGCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCTTTTaa     <  2:387367/141‑1 (MQ=255)
                                                                                                                                       tCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCTTTTaa     >  1:504029/1‑141 (MQ=255)
                                                                                                                                       tCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCTTTTaa     >  2:716762/1‑141 (MQ=255)
                                                                                                                                       tCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCTTTTaa     >  1:687108/1‑141 (MQ=255)
                                                                                                                                        cTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATACATTAAAACTACCAACCTCTTTTAAg    <  1:463371/141‑1 (MQ=255)
                                                                                                                                          tAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCTTTTAAGaa  <  1:52709/141‑1 (MQ=255)
                                                                                                                                          tAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAACTAAAACTACCAACCTCTTTTAAGaa  <  1:860788/141‑1 (MQ=255)
                                                                                                                                          tAAAACACACGTTTTAGCAATAGGGTTTGCGCCTTTTTGCAACCAAAATATTAAGCCCACATTGGAAGCTGTTACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCTTTTAAGaa  <  1:178352/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AATACCTTTCAAAACACGCTTTGAAAATCCGATTTATAAAGGTTTTTCAAGGCTGTTTTTTATGCGCATACATAACCTGATATGTTTTTAATTATGCGCGTGTTATACTAAATTAAATTTTAATACTGCGGGGTGTCTTAAAATGCACATTTTAGTAACAGGGTTTGCGCCTTTTGACAATCAAAATATCAATCCCTCATGGGAAGCTGTGACTCAACTAGAAGATATTATTGGCACACATACAATCGATAAATTAAAACTACCAACCTCTTTTAAGAA  >  CP000730/2850021‑2850299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: