Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	148,490	T→C	11.3%	L105L (TTG→CTG)	galE →	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	148,490	0	T	C	11.3%	109.8 / 4.9	47	L105L (TTG→CTG)	galE	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (19/21);  new base C (2/3);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

ATATGATGCAGTTGAACAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCTTACCATTATCACCATATGCAATAGA  >  CP000730/148354‑148629                                                                                                                                          |                                                                                                                                            atatGATGCAGTTGAACAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGtt                                                                                                                                         <  1:441808/141‑1 (MQ=255)     gatgCAGTTGAACAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTCAGTGTGGTAGaa                                                                                                                                     >  1:83037/1‑141 (MQ=255)      atgCAGTTGAACAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGaaa                                                                                                                                    <  1:65497/141‑1 (MQ=255)      atgCAGTTGAACAAAGGATGAATACATAGCAATTTGATTAGGGTAGTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGaaa                                                                                                                                    >  2:458465/1‑141 (MQ=255)        gtaGTTGAACAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATc                                                                                                                                  <  1:419312/139‑1 (MQ=255)             tGAACAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACAAAGGCAAGATATTCAGATTCTTAGAAATCATTaa                                                                                                                             <  2:424089/141‑1 (MQ=255)                aCAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTCTCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGACTGTTAGAAATCATTaaaaa                                                                                                                          >  2:115504/1‑141 (MQ=255)                aCAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGACTGTTAGAAATCATTaaaaa                                                                                                                          >  2:397210/1‑141 (MQ=255)                aCAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAACATTGTTCGAAATCATTaaaaa                                                                                                                          >  2:317217/1‑141 (MQ=255)                  aaaTCATGAATACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAat                                                                                                                        <  2:55439/141‑1 (MQ=255)                   aaTCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAata                                                                                                                       >  1:209292/1‑141 (MQ=255)                    aTCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAatat                                                                                                                      >  1:268975/1‑141 (MQ=255)                       aTGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGACGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAAGAATTAAAAAAGataaa                                                                                                                   >  1:121178/1‑140 (MQ=255)                           aGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATACTc                                                                                                               >  2:249344/1‑141 (MQ=255)                            gACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCa                                                                                                              >  1:40712/1‑141 (MQ=255)                             aCATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTATTGGTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCat                                                                                                             >  1:90010/1‑141 (MQ=255)                                     aTTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACg                                                                                                     <  2:265577/141‑1 (MQ=255)                                          aTTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTa                                                                                                >  2:409827/1‑141 (MQ=255)                                          aTTATGTAATACATTTAGCAGCATTATTTAGTGTTGCTGAGTAGGTTGAGAAACCTATCTTATATCAAGAACT‑AAAAGTCGTAGCAACATTAAGATTGTTAGAAATCATTCAAAAATATCATAATCATATAAAACGTTGTa                                                                                                >  2:202458/1‑141 (MQ=255)                                                             agcgTTAGTTTGTGTTGCTGAATCGGTTGAGAAACCTATCATATCTCAAGAAAT‑ATACGTCGTAGCAACATTAATTTTGTAAAAAAAAAAAAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCagcagc                                                                             <  2:264145/141‑1 (MQ=255)                                                                 ttagttagTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGtt                                                                         >  1:151118/1‑141 (MQ=255)                                                                      tagtGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAAACAATAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATgg                                                                    <  2:135470/141‑1 (MQ=255)                                                                                gAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCt                                                          >  1:124635/1‑141 (MQ=255)                                                                                gAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGACTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCt                                                          <  1:397210/141‑1 (MQ=255)                                                                                gAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGACTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCt                                                          <  1:115504/141‑1 (MQ=255)                                                                                  gTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGa                                                        <  1:140372/141‑1 (MQ=255)                                                                                       ttGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGc                                                   >  2:122251/1‑141 (MQ=255)                                                                                       ttGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCAGTTTATGGAGAACTTCCTGATTTGc                                                   >  2:171630/1‑141 (MQ=255)                                                                                        tGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGcc                                                  <  2:40712/141‑1 (MQ=255)                                                                                        tGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGcc                                                  <  2:398190/141‑1 (MQ=255)                                                                                        tGAGAAACCTAGCTTATCTCAAGAAATAAAACATC‑TGGTAAAAATACGAAGAAATAAAATCAATAAAAAAGATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGcc                                                  >  2:444346/1‑141 (MQ=255)                                                                                           gaAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTaa                                               <  2:363306/141‑1 (MQ=255)                                                                                                 tatcttatctCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTCGATCTCCTTAAAAAATATAATAATCATATCAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGa                                         <  2:209292/141‑1 (MQ=255)                                                                                                     ttatctCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGatcaa                                     <  1:409827/141‑1 (MQ=255)                                                                                                     ttatctCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTACCTTTGCTTCGTCAGAAGCTGTTTATGGTGATATTCCTGATTTGCCTAAAAGTGataaa                                     >  2:304717/1‑141 (MQ=255)                                                                                                        tctcAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGGGATCTTCCTGATTTGCCTAAAAGTGatcaatca                                  >  1:115323/1‑141 (MQ=255)                                                                                                        actcAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGatcaatca                                  <  2:81940/140‑1 (MQ=255)                                                                                                            aaGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTaa                              >  2:205725/1‑141 (MQ=255)                                                                                                              aaaaT‑AAACGTCGTACCAACATTAAGACTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATc                            <  2:237424/140‑1 (MQ=255)                                                                                                               aaaT‑AAACGTCGTAGCAACATTAAGATTGTTAAAAAAAATAAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCt                           <  2:251523/141‑1 (MQ=255)                                                                                                                 aT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCTTa                         >  1:381814/1‑141 (MQ=255)                                                                                                                    aaaCGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCTTAcc                       <  2:421616/141‑1 (MQ=255)                                                                                                                      aCGTCGTAGCAACTGTAAGATTGGTAGAAATCAGTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCTTACCAt                     >  2:458476/1‑141 (MQ=255)                                                                                                                           gtAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCTTACCATTATCa                <  1:363895/141‑1 (MQ=255)                                                                                                                            tAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGCGCTATCATTAATCTTACCATTATCAc               <  1:458465/141‑1 (MQ=255)                                                                                                                                  cATTAAGATCGTTATAAATCATTAAAAAATATCATACCTATATATTTCGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGACCTTCCTGATTTGCCTAAAAGTGATCCATCATTCATCTTACCACTATCACCATATg         <  2:95910/141‑1 (MQ=255)                                                                                                                                         aTTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCTTACCATTATCACCATATGCAATAGa  <  1:143618/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                                            ATATGATGCAGTTGAACAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCATTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAAT‑AAACGTCGTAGCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAATCATATAAAACGTTTTATCTTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCATTAATCTTACCATTATCACCATATGCAATAGA  >  CP000730/148354‑148629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑