Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9100AG100.0% 143.8 / NA 43intergenic (‑1/‑121)USA300HOU_0653/USA300HOU_0654ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/27);  total (16/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAA  >  CP000730/707775‑708045
                                                                                                                                       |                                                                                                                                       
gATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGttt                                                                                                                                    <  2:266932/141‑1 (MQ=255)
       gAAAATTTACCAGCTCCATTTGGGGCCATGATACCAATTATTTTGCCGCGTATTGGTATCGATAAGGAAATTTATTTAACTACATGCTGATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAcctc                                                                                                                             <  1:108114/141‑1 (MQ=255)
           aTTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTGTCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACACGCGTTTATTCCTCCTac                                                                                                                         >  1:33646/1‑140 (MQ=255)
               aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaa                                                                                                                     >  1:122091/1‑141 (MQ=255)
                ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaaa                                                                                                                    >  1:102614/1‑141 (MQ=255)
                              ggccATGATGCCAAGTATTGCGCGTCGTACTGGTATCGATAAGTAAATGTTTTTAAGTACATGCTTATTACCTAAAATCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                      <  2:77018/139‑1 (MQ=255)
                              gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                      <  1:396879/141‑1 (MQ=255)
                              ccccATGATACCAATTATTTCGCCGCGTACTGGTATCTATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                      <  2:299439/140‑1 (MQ=255)
                               cccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCt                                                                                                     <  2:377983/141‑1 (MQ=255)
                                 cATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                   <  1:352061/141‑1 (MQ=255)
                                         cAATTATTTCGCCGCGTAATAGTATCAACAAGGAAATGTTTTTAAGTACATGCTTTTTACCTAAAAACAGATTTAAATCTTTTTTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGt                                                                                           <  1:7449/141‑1 (MQ=255)
                                          aaTTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGta                                                                                          <  1:179304/141‑1 (MQ=255)
                                            ttattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                                                        <  1:405540/141‑1 (MQ=255)
                                             tattTCGCCGCGTACTGGTATCCATAAGGAAATGTTGTTAAGTACATGCGTATTACCTAAAAACAGATTTAAATATTTTGTTTCTAACAAGCGTTTATACCTCCTGATTAAAAGTTGAGGCGAACCTAATTAATAGTataa                                                                                       <  2:41442/141‑1 (MQ=255)
                                                 tCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                                                                   >  1:174705/1‑141 (MQ=255)
                                                   gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTCAAGGCATATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                                                 >  1:456526/1‑141 (MQ=255)
                                                   gccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACt                                                                                 >  2:363315/1‑141 (MQ=255)
                                                       cgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTTTATAATAAACTgaga                                                                             >  2:326607/1‑141 (MQ=255)
                                                       cgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgaga                                                                             >  1:225620/1‑141 (MQ=255)
                                                           cTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAatat                                                                         <  2:176206/141‑1 (MQ=255)
                                                             ggTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAttt                                                                       <  2:94859/141‑1 (MQ=255)
                                                              ttATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTACATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTa                                                                      <  2:361482/140‑1 (MQ=255)
                                                              gTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTa                                                                      <  2:82805/141‑1 (MQ=255)
                                                                aTCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATc                                                                    <  1:363315/141‑1 (MQ=255)
                                                                          aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATGTATCCTGTCAAGTa                                                          <  2:313735/141‑1 (MQ=255)
                                                                          aaaTGTTTTTAAGTACAGGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                          <  2:174210/141‑1 (MQ=255)
                                                                            aTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                        >  2:70260/1‑141 (MQ=255)
                                                                                 tttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGCCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                                                   <  2:421206/141‑1 (MQ=255)
                                                                                    aaGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTGTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATTTCAAGTAAATTCGTGat                                                >  1:45876/1‑141 (MQ=255)
                                                                                     aGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGata                                               <  1:135720/141‑1 (MQ=255)
                                                                                        aCATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataa                                            <  1:294225/141‑1 (MQ=255)
                                                                                             cTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataatataa                                       >  2:1011/1‑140 (MQ=255)
                                                                                             cTTACTACCTAGAAACAGTTTTAAATCTTCAGCACCTTACAGGCGGTTATACCTGCTAATTAAATTTTTAGGCTGACCTAATTAATTGTATACTAATCTAAGAATATTTATCATGTCAAGTAAATTCCTGATGTAATATAg                                       >  2:119429/1‑141 (MQ=255)
                                                                                              ttattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGa                                      >  1:442943/1‑141 (MQ=255)
                                                                                                  taCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAat                                  <  2:174705/141‑1 (MQ=255)
                                                                                                         aaaCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTAtgtg                           <  2:238407/141‑1 (MQ=255)
                                                                                                           aCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAg                         <  2:260187/141‑1 (MQ=255)
                                                                                                             aGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGt                       <  1:244467/141‑1 (MQ=255)
                                                                                                                      tCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATg              <  2:28342/141‑1 (MQ=255)
                                                                                                                        ttttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTCTCCTGACGTATAGATCCGTGATATAGTATAGACAGTAAATGTGACGTGCAAGTATGtt            >  1:451956/1‑141 (MQ=255)
                                                                                                                        ttttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGtt            >  2:265035/1‑141 (MQ=255)
                                                                                                                                 tAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaaga   <  2:83678/141‑1 (MQ=255)
                                                                                                                                  aaCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTACTATAAGATAAAAAACCGATGTGAGGTGAAACTATGTTAACTgaagaa  >  2:95875/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAA  >  CP000730/707775‑708045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑