| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,233,504 | T→A | 100% | V470D (GTT→GAT) | USA300HOU_1157 → | non‑specific serine/threonine protein kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,233,504 | 0 | T | A | 100.0% | 105.2 / NA | 34 | V470D (GTT→GAT) | USA300HOU_1157 | non‑specific serine/threonine protein kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (19/15); total (19/15) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAA > CP000730/1233368‑1233634 | ggTGAACGTGTTGAAGGTGGTGACAGTGTTGAGGTTGGTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACg < 1:441123/141‑1 (MQ=255) gTGAACGTGTTGAACGTGGTGACAGTTTTGATGTTGTTATATCAAAGGGCGCTGGAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTACAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGa < 2:335458/141‑1 (MQ=255) gAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGAtt < 1:167140/141‑1 (MQ=255) aCGTGTTGAACGTGGTGACAGTGTTGATGTGGTTATATCAAAGGGCCCGGAAAAGGTTAAAGTGCCAAATGTCCTTGGTTTACCTAAGGATGAAGCGTTGCAGAAATTAAAGTCGTTAGGTCTTAAAGATGATACGGTTGa > 2:302539/1‑141 (MQ=255) tgtTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTAATTGGTTTACCTAAGGAGGAAGACTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGaaaa > 1:352335/1‑141 (MQ=255) aaCGAGGTGACAATGATTATGTTGTTATATCAAAAGGCGCTGAAAAGGTCAAAATGCCAAATGTCAGTGGTTTACCTAAGGAAGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGtat < 2:142327/141‑1 (MQ=255) gACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGCGGAAGCCTTGCAGAAATTAAAATCGTTGGGTCTTAAAGATGATACGATTGACAAAGTATATcataat > 1:49594/1‑141 (MQ=255) ttGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAgcgc > 2:257179/1‑141 (MQ=255) aaaGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGc < 1:401366/141‑1 (MQ=255) aaGGACCCTGAAAAGGTTAAAGTGCCAAATGGCATTGGTGTACCTAAGGTGGAAGCCTTGCAGAAATTAAAATCGTTAGTTCTTAAAGAAAAAAAAAAAAAAAAAGTATAGAATAATCAAGCGCCAAAAGGATACAATGCa < 2:280971/141‑1 (MQ=255) aGGGCCCTGAAAAGGTTAAAATGCCAACTGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAGAAGTATAGAATAGTAAAGACCCAGAAGAAAAAATTCCaa > 2:383380/1‑141 (MQ=255) aGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCaa > 1:110060/1‑141 (MQ=255) ggCGCTGAAAAGGTTAAAATGCCGAATGTTATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTTATATCGTTATGTCTTAAAGATGATACGATTGTAAGAGATTATACTAATCAAGAGACAGAAGGATACATTGCaaga > 2:22900/1‑139 (MQ=255) cccTGAAAATGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAACGGATACATTGCAAATCa > 1:338742/1‑141 (MQ=255) cTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCaaa > 2:145600/1‑141 (MQ=255) ttaaaaGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGGTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAg < 2:356752/139‑1 (MQ=255) tGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCCAAAGAATACATTGCAAATCaaaa > 2:72470/1‑140 (MQ=255) aaaaGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAgtg < 1:18314/141‑1 (MQ=255) aaaGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAACTTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAgtgt > 1:358882/1‑141 (MQ=255) tAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAGAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGc < 1:38417/141‑1 (MQ=255) aaaTGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATAAAGCGCTATTAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGa < 1:243143/141‑1 (MQ=255) gTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATc < 1:132903/141‑1 (MQ=255) tCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAACCGACAAATGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCg > 1:380348/1‑141 (MQ=255) aCCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAGGTGTAACCGCAAATACTGAAATCGCTATTCATGa > 1:91902/1‑141 (MQ=255) aaGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCt < 1:413897/141‑1 (MQ=255) aggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTACAAATCAAAGTGTAACCGCAAATACTGAAAGCGCTATTCATGATTCTAata < 2:91096/141‑1 (MQ=255) aaGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTa > 2:25322/1‑141 (MQ=255) aTTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGAATCTAATATTAAACTATATGaacc > 2:126570/1‑139 (MQ=255) ttAAAATCGTTAGGTCTTAAAGATGATACGATTGAACCAGTACATAAGAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCGATTCATGATTCTAATATTAAACTATATGAATCt > 1:435768/1‑141 (MQ=255) tAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTa > 1:188077/1‑141 (MQ=255) aGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTaa > 2:162903/1‑141 (MQ=255) gTCTTAAAGATCATACGATTGAAAAAGTATATAATAAGCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGc < 2:352335/141‑1 (MQ=255) tCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCa < 1:270771/141‑1 (MQ=255) cTTAAAGATGATACGATTGAAAAAGTATATAGTAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGACATTAAGCaa > 1:113878/1‑141 (MQ=255) | GGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAA > CP000730/1233368‑1233634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |