Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 162.3 / NA 47I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/24);  total (23/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCG  >  CP000730/1416325‑1416595
                                                                                                                                     |                                                                                                                                         
aaCTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTTGCCGTCGTTTAAAACATAATTGTACTg                                                                                                                                    <  1:339789/141‑1 (MQ=255)
  cgtactCACAGTAACCCTTCTTTAATTGTGTTCGATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAc                                                                                                                                  <  1:224822/136‑1 (MQ=255)
   tCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACtcggg                                                                                                                                 <  2:119656/141‑5 (MQ=255)
   tCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACa                                                                                                                                 <  2:244725/141‑1 (MQ=255)
     aaTCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTTCTGACAat                                                                                                                               >  1:254063/1‑141 (MQ=255)
          tAGTAATCCCTCTTTGATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCATTGATTTGACCTTCTTTTAAAACACAATTGTACTGACAATATTTc                                                                                                                          <  1:280517/141‑1 (MQ=255)
            gTAATCTCTTGTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCaa                                                                                                                        >  1:339044/1‑141 (MQ=255)
              aaTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATa                                                                                                                      >  1:155811/1‑141 (MQ=255)
                 cccTCTTTAAGTGTGTTCTATATGTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCACGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACAt                                                                                                                   <  1:278190/141‑1 (MQ=255)
                           ttGTGTTCTATATTTGAATAGACGTTCAGTTTACGGATGCAAATGCTCATACGTGAAATGATTAATATTACCTCGTTCAATGATGTGACCTTCTGTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                         <  2:105870/141‑1 (MQ=255)
                               gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgt                                                                                                     >  2:321415/1‑141 (MQ=255)
                                ttCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtg                                                                                                    <  2:155811/141‑1 (MQ=255)
                                  cTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgtg                                                                                                  >  2:460545/1‑141 (MQ=255)
                                    atatTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGat                                                                                                >  2:32238/1‑141 (MQ=255)
                                         tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                           >  2:307479/1‑141 (MQ=255)
                                                   ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGCTTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCACTACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                 >  1:401101/1‑141 (MQ=255)
                                                   ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                 >  2:415562/1‑141 (MQ=255)
                                                     cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                               >  2:53418/1‑141 (MQ=255)
                                                       gTATACGGATGCAACTGCTCATACTTGAAATGATTATTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACCATATTTCAATACATGACTTCAGTTATGTGTGATAATAAATAATCTTTTACCa                                                                             >  2:371286/1‑141 (MQ=255)
                                                       gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                             >  1:392642/1‑141 (MQ=255)
                                                       gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                             >  2:62619/1‑141 (MQ=255)
                                                         ataCGGATGCAAATGCTCATGCTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATg                                                                           >  1:280418/1‑141 (MQ=255)
                                                           aCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                         <  1:262048/141‑1 (MQ=255)
                                                            cGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATCTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATCAATAATGTTTGACCATGTTc                                                                        <  2:447015/141‑1 (MQ=255)
                                                               aTGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTaa                                                                     <  1:426343/141‑1 (MQ=255)
                                                                   aaaTGCTCATACTTGAAATGATTAATATTAGCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACa                                                                 <  2:339664/141‑1 (MQ=255)
                                                                      tGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAata                                                              >  1:244839/1‑141 (MQ=255)
                                                                       gCTCATACTTGAAATGATTAACATTACCTCGTTCAATTATTTGACCTTCTTTTAAAACATAATTGTCATGACAATACTTCAATACATGACTTAAGTTATGTGTGATAATAAATCATGTTTGACCATGTTCTAATCCAatat                                                             <  2:116023/141‑1 (MQ=255)
                                                                          cATACTTGAAATTATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCt                                                          <  2:396428/141‑1 (MQ=255)
                                                                                       aTTAATATTACCTCGTTTAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCAt                                             <  2:195144/141‑1 (MQ=255)
                                                                                        ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATc                                            >  1:165755/1‑141 (MQ=255)
                                                                                        ttAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGGGTGATAATAAATAATGTTTGGCCATGTTCGAATAAAATGTGCTGTAATAAACCCAta                                            >  1:365908/1‑140 (MQ=255)
                                                                                           atagTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACGTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACt                                         <  1:194862/141‑1 (MQ=255)
                                                                                             atTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATGCAATATGCTGTAATAAATCCATCACTTg                                       <  1:299041/141‑1 (MQ=255)
                                                                                              ttACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATCCAATATGCTGTAATAAATCCATCACTTGa                                      >  2:164550/1‑141 (MQ=255)
                                                                                               tACCTCGTTCAATGACTTGACCTTCTTTTAAAACATATTTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTGTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGAt                                     <  1:325400/141‑1 (MQ=255)
                                                                                                 ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATTCTGTAATAAGTCCATCACTTGATTa                                   <  1:230365/141‑1 (MQ=255)
                                                                                                   tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAcc                                 <  2:52495/141‑1 (MQ=255)
                                                                                                    cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCg                                <  1:403652/141‑1 (MQ=255)
                                                                                                     gTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGt                               <  1:460545/141‑1 (MQ=255)
                                                                                                      ttCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGtt                              >  1:295355/1‑141 (MQ=255)
                                                                                                                     ttcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCCCTTGATTACCGTTCAAAGCATCCAatga               >  1:170506/1‑141 (MQ=255)
                                                                                                                         tttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCa           >  1:167808/1‑141 (MQ=255)
                                                                                                                             aaaCATAATTGTACTGACAATATTTGAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAAGGATGCAACTg       >  1:230502/1‑141 (MQ=255)
                                                                                                                             aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg       <  1:251847/141‑1 (MQ=255)
                                                                                                                             aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg       <  1:373883/141‑1 (MQ=255)
                                                                                                                                  tAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTct  >  1:301974/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                         
AACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCG  >  CP000730/1416325‑1416595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑