Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,486,271 T→C 9.3% T5251T (ACA→ACG)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,486,2710TC 9.3% 112.6 / 3.5 43T5251T (ACA→ACG) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base T (16/23);  new base C (2/2);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

TTGCGTTATTTAATTCTGTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTTGCTGCAGTAACTGC  >  CP000730/1486132‑1486411
                                                                                                                                           |                                                                                                                                            
ttGCGTTATTTAATTCTGTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTg                                                                                                                                             >  2:414026/1‑141 (MQ=255)
       aTTTAATCCTGTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGc                                                                                                                                      <  2:454401/141‑1 (MQ=255)
        tttAATTCTGTTGCTTTTGCAGTTTCTAGCTTTCCACCCGATACTGTTGTTGCACCATCAATACCACCCCTTAACGCATCTCTGTGTCCATTGTTGACAGATCTTAAGTTACTCAAGAGTTCTTTCGCCGCTGTCTTAGCt                                                                                                                                     >  2:34716/1‑141 (MQ=255)
             ttCGGTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACGTTTGTGGCACCAGCAATGCTACGCGTTAATGCATCTTTTTGTGGATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGATGTTGTCTTAGCTTGAGc                                                                                                                                <  2:83213/141‑1 (MQ=255)
              tCTGTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCt                                                                                                                               >  1:390919/1‑141 (MQ=255)
               cTGTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTa                                                                                                                              <  2:16365/141‑1 (MQ=255)
                 gTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAg                                                                                                                            <  1:129559/141‑1 (MQ=255)
                 gTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAg                                                                                                                            <  2:27733/141‑1 (MQ=255)
                          gCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCgcgcc                                                                                                                   >  1:340817/1‑140 (MQ=255)
                            aGTTTCTTGTTCTCCACCTGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCAcc                                                                                                                 <  2:381118/141‑1 (MQ=255)
                            aGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGGTGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCAcc                                                                                                                 <  1:453225/141‑1 (MQ=255)
                            aGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCAcc                                                                                                                 <  1:77533/141‑1 (MQ=255)
                             gTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCa                                                                                                                <  2:394648/141‑1 (MQ=255)
                               ttCTTGATTTACACCAGCTACTGTTGTTGCGCCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCAtt                                                                                                              <  2:366794/141‑1 (MQ=255)
                               ttCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGGTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCAtt                                                                                                              >  1:436033/1‑141 (MQ=255)
                                     aTTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGc                                                                                                        >  1:266458/1‑141 (MQ=255)
                                         acacCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGt                                                                                                    <  2:334207/141‑1 (MQ=255)
                                         acacCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCACACCATTTAATGCCTGt                                                                                                    <  1:407581/141‑1 (MQ=255)
                                                        gttgCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCCCt                                                                                     >  1:103510/1‑141 (MQ=255)
                                                         ttgCACCATCAATGCTACGCGTTGACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACtt                                                                                    >  2:330529/1‑141 (MQ=255)
                                                         ttgCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCTGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACtt                                                                                    >  2:350942/1‑141 (MQ=255)
                                                          tgCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTtg                                                                                   <  2:378667/141‑1 (MQ=255)
                                                                  cAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTa                                                                           >  2:428818/1‑141 (MQ=255)
                                                                         aCGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGt                                                                    >  2:355754/1‑141 (MQ=255)
                                                                         aCGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTCGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGt                                                                    >  2:449092/1‑141 (MQ=255)
                                                                         aCGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTCGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGt                                                                    >  2:134303/1‑141 (MQ=255)
                                                                            cgTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAAt                                                                 <  2:177757/141‑1 (MQ=255)
                                                                                       ttttttGTGCAATTTTAATTGATGGTAAGTAATTCAAGTTCTTTTTCGCAATTCTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTCCTATAGTTATCACGGCTTATACTAATTCCGTAATAGAATTTGGtt                                                      <  2:191487/140‑1 (MQ=255)
                                                                                       cTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTGGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGtt                                                      <  1:330529/141‑1 (MQ=255)
                                                                                       cTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGtt                                                      >  1:165151/1‑141 (MQ=255)
                                                                                          tttGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCa                                                   >  1:212625/1‑141 (MQ=255)
                                                                                               gCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGtt                                              >  1:411486/1‑141 (MQ=255)
                                                                                                 aTTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTCGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTCCTTGCGTAATAGTATTTGGTTCCATCGTTgg                                            <  1:134303/141‑1 (MQ=255)
                                                                                                 aTTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTCGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTgg                                            <  1:449092/141‑1 (MQ=255)
                                                                                                   tctcaaTTGATTGTACGTTAGTCAAGTTGGTTTCCGCCGTTGTCTTAGCTTGAGCTAAGTTTCGCGCCCCCTTTAATGCCTGTTCTTTCGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGt                                          <  2:429761/137‑1 (MQ=255)
                                                                                                    gTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGtt                                         <  1:428818/141‑1 (MQ=255)
                                                                                                      tAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCg                                       <  1:366827/141‑1 (MQ=255)
                                                                                                         ttGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATg                                    <  2:120342/141‑1 (MQ=255)
                                                                                                          tGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGt                                   <  1:414026/141‑1 (MQ=255)
                                                                                                                tAAGTTATTCAAGTTGTTTTTCGCAGGTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGAtt                             <  2:170576/141‑1 (MQ=255)
                                                                                                                         cAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGc                    >  1:272386/1‑141 (MQ=255)
                                                                                                                                     cGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTTGCTGCAGt        <  1:124311/141‑1 (MQ=255)
                                                                                                                                         gTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTTGCTGCAGTAACt    >  2:241961/1‑141 (MQ=255)
                                                                                                                                           tGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTCGCTGGAGTGCCTGc  <  2:101139/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TTGCGTTATTTAATTCTGTTGCTTTTGCAGTTTCTTGATTTACACCAGCTACTGTTGTTGCACCATCAATGCTACGCGTTAACGCATCTTTTTGTGCATTGTTAATTGATGTTAAGTTATTCAAGTTGTTTTTCGCAGTTGTCTTAGCTTGAGCTAAGTTTCGCGCACCATTTAATGCCTGTTCTTTAGTTGTCACTTGTGATGTTACTTGCGTAATAGTATTTGGTTCCATCGTTGGGTTCGATGTTTGATTAATGATTGCTTTTGCTGCAGTAACTGC  >  CP000730/1486132‑1486411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑