Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,352,815	G→A	100%	A92V (GCA→GTA)	rpsI ←	ribosomal protein S9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,352,815	0	G	A	100.0%	80.2 / NA	28	A92V (GCA→GTA)	rpsI	ribosomal protein S9
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/11);  total (17/11)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAAT  >  CP000730/2352685‑2352951                                                                                                                                   |                                                                                                                                         ccaCAATTAACGTTATGAAAATTGAGGTGAACGACGAGCCGCTATAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCtaataa                                                                                                                                <  1:129468/139‑1 (MQ=38)      aTTAACGTTTTGAGAGTTGAGGTGACCGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCAc                                                                                                                           >  1:230862/1‑141 (MQ=255)        tAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGa                                                                                                                         >  2:101979/1‑141 (MQ=255)        tAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTCTAAAGAACCCCTGTATCCAGGACCTACTTCTAATAATGCACGa                                                                                                                         >  2:101909/1‑141 (MQ=38)         aaCGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAg                                                                                                                        >  1:351334/1‑141 (MQ=255)                                  acgaGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATagcttgagc                                                                                               >  1:57290/1‑141 (MQ=255)                                              aaGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGaa                                                                                   <  2:376957/141‑1 (MQ=255)                                               aGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGaaa                                                                                  <  2:415848/141‑1 (MQ=255)                                               aGACCTGGTTTTTAACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGaaa                                                                                  <  1:52177/141‑1 (MQ=255)                                                 cccTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAacc                                                                                <  2:153537/140‑1 (MQ=255)                                                 aCCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAacc                                                                                <  1:375913/141‑1 (MQ=255)                                                       ttgtttACGTTCATAAAAACGGTGGTCACGATGAAGCAATCCAGCGCGTTTGAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGAGTCCGTGACGTATATCTTGAGCTTGTCCAGTGAAaccaccacc                                                                          <  2:358431/138‑1 (MQ=255)                                                          tttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGCATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATg                                                                       >  2:187466/1‑141 (MQ=255)                                                               gTTCTTTCATACTTGGGTCACGAGTAAGTAATCAAGCGCGTTTTAAAGAAACTCTGTATTCAGGATCTAATTCTAATAATGCAGGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCATTGAAACCACCACCATGAACGt                                                                  >  2:147847/1‑141 (MQ=255)                                                               gTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGt                                                                  <  1:45040/141‑1 (MQ=255)                                                                      cATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACATTGCCTaa                                                           >  1:428775/1‑141 (MQ=255)                                                                            tGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcagc                                                     >  2:82715/1‑139 (MQ=255)                                                                                  aCGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCCCCATAAACGTTAGATAAAAcagcatcggt                                               >  2:53503/1‑137 (MQ=255)                                                                                   cGAGTAAGTAATCCAGCGCGTTTTAAAGACAGGTTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTa                                              >  1:457987/1‑141 (MQ=255)                                                                                    gAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGAGCTACTTCTGATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCCCCATGGACGTTAACTAAAACATCATCGTTAc                                             >  2:2346/1‑141 (MQ=255)                                                                                      gtaagtaaTCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCt                                           <  2:351334/141‑1 (MQ=255)                                                                                                         ttAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaa                        >  1:12171/1‑141 (MQ=255)                                                                                                              gAACCTCTGTATTCAGGATCTACTTCTTATATTGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTTACTTACAttcgatggt                   <  2:95493/141‑9 (MQ=255)                                                                                                                 ccTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTACAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttgg                >  1:170766/1‑141 (MQ=255)                                                                                                                 ccTCTGTATTCAGGATCTACTTCTAATAAGGCCCGAGCGAGTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggttgg                <  2:360257/141‑1 (MQ=255)                                                                                                                     tGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTa            >  1:372493/1‑141 (MQ=255)                                                                                                                       tATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCCCCATGAACGTTAACTAAAACATCATGGTTACCTTTAGTTTCCGTTACATCAAATGGTTGGCTTAAg          >  2:85514/1‑141 (MQ=255)                                                                                                                               aTCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAACTGGTTGGTTTAAGTCTaaagt  >  2:93132/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                         CGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGTCTAAAAT  >  CP000730/2352685‑2352951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑