Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,504,989 T→C 6.6% intergenic (+293/+111) USA300HOU_2365 → / ← gltT hypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,504,9890TC 6.6% 240.6 / 2.8 109intergenic (+293/+111)USA300HOU_2365/gltThypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter
Reads supporting (aligned to +/- strand):  ref base T (44/50);  new base C (3/8);  total (49/60)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCTTT  >  CP000730/2504850‑2505129
                                                                                                                                           |                                                                                                                                            
ttGCGCATGCATATAAGCCCCTTGCCACCTGGGTTCGCTGCGCTCCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACATCCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTc                                                                                                                                             <  2:307781/141‑1 (MQ=14)
ttGCGCATGCATAACAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGCTTCGCCTGCGCTTTAGCGCATGCATAATAGTc                                                                                                                                             <  1:476739/141‑1 (MQ=255)
ttGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTc                                                                                                                                             <  1:397773/141‑1 (MQ=255)
ttGCGCAGGCATAAAAGCCCCCAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTc                                                                                                                                             <  1:91372/141‑1 (MQ=255)
cttctaatgcatAAACTCCCCTAACAACCTGAGGTAACTCCACTCCCGTTCGCCCTCGCCTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTACAGATCTCCTTCGCTTTAGCGCATGCACAAAAGTc                                                                                                                                             <  2:440632/135‑1 (MQ=11)
 tGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTcc                                                                                                                                            >  1:369616/1‑141 (MQ=255)
     catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGCGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaa                                                                                                                                        <  1:351165/141‑1 (MQ=255)
     catgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaa                                                                                                                                        <  2:618029/141‑1 (MQ=255)
      atgcatAAAAGCCCCTAACCACCTGCGGTCTCTGCGCTCCGGTTCCCCTGCGCTGTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAATTCCCTaac                                                                                                                                       <  2:490443/141‑1 (MQ=14)
       tgcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaaca                                                                                                                                      >  1:40659/1‑141 (MQ=255)
        gcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaacaa                                                                                                                                     >  1:659185/1‑141 (MQ=255)
        gcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaacaa                                                                                                                                     >  1:626584/1‑141 (MQ=255)
        gcatAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCGTCGTTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                                                                                     >  1:300988/1‑141 (MQ=255)
           tAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAAccg                                                                                                                                  >  2:581804/1‑140 (MQ=255)
            aaaaGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTaacacacaa                                                                                                                                 >  2:66796/1‑136 (MQ=255)
            aaaaGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGGCCCTAACAAcccg                                                                                                                                 >  2:310316/1‑139 (MQ=255)
                gCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCCCATGGCTTAGGTTCGCCTGGGCTTTAGCGCATGCATAGAAGTCCCAAACAACCAGCGGt                                                                                                                             >  2:584067/1‑141 (MQ=14)
                  cccccaccacCTGAAATCACTGCCCTCCGCATCCCCCGCCCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGATCACTACGCTCCGGTTCGCCCGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCa                                                                                                                           <  2:118479/133‑1 (MQ=14)
                  cccTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTcg                                                                                                                           >  1:619934/1‑140 (MQ=255)
                   ccTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCAc                                                                                                                          >  1:202056/1‑141 (MQ=255)
                     tAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCGGAGGTCACTa                                                                                                                        >  1:323985/1‑141 (MQ=255)
                      aacaacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTAc                                                                                                                       >  1:238275/1‑141 (MQ=255)
                       acaacCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTAct                                                                                                                      >  1:469706/1‑140 (MQ=255)
                          acCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCtt                                                                                                                   >  1:296328/1‑141 (MQ=255)
                              ggggTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGt                                                                                                               <  1:209823/139‑1 (MQ=255)
                              gAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGt                                                                                                               <  1:443774/141‑1 (MQ=255)
                              gAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGt                                                                                                               <  1:82246/141‑1 (MQ=255)
                              gAGGTCACTGCGCTCCCGTTCGGCTGCGCTTTAGCGCATGCATAAACGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCACCGTCCCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGt                                                                                                               <  1:185836/141‑1 (MQ=17)
                               aGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCGCTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGtt                                                                                                              >  2:427301/1‑141 (MQ=255)
                               aGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCAGGCATAAAAGTCCCTAACAACCTAAGGTCACAACGCTTCCGtt                                                                                                              >  1:287211/1‑141 (MQ=255)
                                  tCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCGGAGGTCACTACGCGTCCGGTCGc                                                                                                           >  2:392695/1‑141 (MQ=255)
                                   cACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGcc                                                                                                          >  1:466503/1‑141 (MQ=255)
                                    aCTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCt                                                                                                         >  1:556447/1‑141 (MQ=255)
                                       ccgcTCCGGGTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGCGGTCACTACGCTTCCGTTCGCCTgcg                                                                                                      <  2:413270/140‑1 (MQ=255)
                                       ccacTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTgcg                                                                                                      <  2:214883/138‑1 (MQ=255)
                                          cttcccttCGCCTGCGCTTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCtt                                                                                                   <  2:314939/135‑1 (MQ=255)
                                          cTCCGGTTCGCCTGCGCCTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCtt                                                                                                   <  2:555760/141‑1 (MQ=255)
                                           tgcGGTTCGCCTGCGCTTTAGCGGAGGCATAAAGGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCttt                                                                                                  <  2:198084/139‑1 (MQ=255)
                                             cGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAg                                                                                                <  1:602975/141‑1 (MQ=255)
                                                   gCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatg                                                                                          <  1:662202/141‑1 (MQ=255)
                                                   gCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCGGCGCTTTAGCgcatg                                                                                          <  1:495428/141‑1 (MQ=255)
                                                   cccTGGGCTTTGGCGTATGAATAAACGCCCCTAACAACCTGGTGTCACTACGCTTCGGTTCTCCTGCGCTTTAGCGCATGCATAAATTTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatg                                                                                          <  2:107756/140‑1 (MQ=14)
                                                     cTGGGCTTTAGCGCATGCATTAAGGCCCCCAACCACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgca                                                                                        <  2:250861/141‑1 (MQ=14)
                                                     cTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCgcatgca                                                                                        <  1:209918/141‑1 (MQ=255)
                                                       gcgcTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATa                                                                                      <  2:507072/141‑1 (MQ=255)
                                                            ttAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGc                                                                                 >  1:357859/1‑141 (MQ=255)
                                                              aGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAGCGCACTAACCACCTGAGGTCACCACGCTTCCGTGCGCCTGCGCTTTAACGCATCCCTAAAAGcac                                                                               >  2:402547/1‑139 (MQ=14)
                                                              aGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGccc                                                                               >  1:563532/1‑141 (MQ=255)
                                                              aGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGccc                                                                               >  1:76190/1‑141 (MQ=255)
                                                              aGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGacc                                                                               >  1:215882/1‑141 (MQ=255)
                                                               gcgcATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGcccc                                                                              >  1:507072/1‑141 (MQ=255)
                                                                     gcatACAAGCCCCTAGCCACCTGCGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTATCAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                        <  2:119628/141‑1 (MQ=14)
                                                                     gcatAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaa                                                                        <  1:101107/141‑1 (MQ=255)
                                                                     gcatAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTACCGCATGCATaaagccacacaccca                                                                        >  2:564309/1‑129 (MQ=14)
                                                                      catAAAAGCCCCTAACAACCTTGGGTTACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTaacaac                                                                       <  2:198933/141‑1 (MQ=255)
                                                                        tAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCa                                                                     >  1:598942/1‑141 (MQ=255)
                                                                              ccccTAACAACCTGAGGTCACTAAAATACCTGTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGtt                                                               >  1:489622/1‑141 (MQ=17)
                                                                                cccaacaacCCGAGCTCACTACCCTTCGGCTCGCCTGCGCTTTACCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGt                                                             <  1:207928/138‑1 (MQ=17)
                                                                                ccTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGt                                                             >  1:79382/1‑141 (MQ=255)
                                                                                ccTAACAACCTGAGGTCACTACGCTTCGGTTCACCTGAGCTTAAACCCCTGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGt                                                             >  1:392328/1‑141 (MQ=17)
                                                                                  tAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCTTGCATAGATGCCCCTTACAACTGCAGGTTGTat                                                           >  2:586607/1‑140 (MQ=14)
                                                                                    acaacCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGGGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAgg                                                         >  1:302550/1‑141 (MQ=255)
                                                                                       acccggtttctctccGCTTCCGCTCGCCTGCGCTGTAGCGCATGCATAAAAGTCCCCAACAACCTGAGGTCACTACGCTTCCGTTCCCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGc                                                      <  2:155879/127‑1 (MQ=17)
                                                                                        ccTGCGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCTCTAACAACCAAAGGTTGTAAGGGGCt                                                     <  2:398119/141‑1 (MQ=255)
                                                                                        ccTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCt                                                     <  2:71100/141‑1 (MQ=255)
                                                                                         cTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTCCCGTTCGCCTGCGCTTTAGCGCATGAATAAAAGCCCATAACAATCAACGGTTGTAAGGGGCtt                                                    >  2:655739/1‑141 (MQ=17)
                                                                                                cACTGCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGt                                             <  2:509634/141‑1 (MQ=255)
                                                                                                   tACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAg                                          <  2:369616/141‑1 (MQ=255)
                                                                                                    aCGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGa                                         >  2:525472/1‑141 (MQ=255)
                                                                                                     cGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGAt                                        <  1:392695/141‑1 (MQ=255)
                                                                                                     cGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGAt                                        <  2:653065/141‑1 (MQ=255)
                                                                                                     cGCTTCCGTTCGCCTGCGCTTTAGCGCATCCATAAAAGTCCCTAACAACCTGACGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGAt                                        <  2:200494/141‑1 (MQ=255)
                                                                                                     cGCTTCCGTTCGCCTGCGCCTCCGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGAt                                        <  2:187890/141‑1 (MQ=255)
                                                                                                      gCTTCGGTTCGCCTGCGCTTCAGCGCATGCATAAAAGTCCCTAACAACCTGAGCTCACTACGCTTCCGTTCGCCTGCGCTTAAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATa                                       <  1:492372/141‑1 (MQ=255)
                                                                                                          cGGTTCCCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACTCTTCCGTTCGCCTGCGCTTTAGCGCATGCATATAAGCCCCTAACAACCAACGGTTGTAAGGGGCGTTAAATGTCAGATAGTAt                                   <  2:187361/141‑1 (MQ=18)
                                                                                                               cgtccgcccTTTATCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTCCCCTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAtttttt                              <  2:615917/133‑1 (MQ=21)
                                                                                                               cGCCTTGGCTTTAGCTCATGCATAAAAGCCCCTTACAACCTGAGTTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAtttttt                              <  2:362664/141‑1 (MQ=21)
                                                                                                               cGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAtttttt                              >  1:446919/1‑141 (MQ=255)
                                                                                                               cGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCAGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAtttttt                              >  2:37858/1‑141 (MQ=255)
                                                                                                               cGCCTGCGCTTCAGCGCATGCATAAAGGTCCCGAACTAACTGAGGTCACTACCCTTCCGTTCGCCTGCGCTTTAGCGCAGTCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTAtttttt                              <  2:55452/141‑1 (MQ=21)
                                                                                                                  ccgcgcTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTaaa                           <  1:91760/139‑1 (MQ=255)
                                                                                                                  cTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACTACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTaaa                           <  1:314053/141‑1 (MQ=255)
                                                                                                                    ccgcCTTAGCGCATGCATAAAAGGCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAAtt                         <  2:296328/140‑1 (MQ=255)
                                                                                                                     cgcTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTg                        >  2:445441/1‑141 (MQ=255)
                                                                                                                      gcTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTgt                       >  2:649205/1‑140 (MQ=255)
                                                                                                                      gcTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGa                       >  1:275748/1‑141 (MQ=255)
                                                                                                                       cTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCATAGGTTGTCAGGGGCTTTAAATGTCAGCTATTATTTTTTAAATTGAt                      <  1:371057/141‑1 (MQ=255)
                                                                                                                          tAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGAtttt                   >  1:623730/1‑141 (MQ=255)
                                                                                                                           aGCGCATGGAAAAAAGGCCCTAACAACCTGAGATCAATACCCTGCCGTGCGCCTGCGCTTTAGCGCATGCATAAAAGCACCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTa                  <  1:16124/141‑1 (MQ=25)
                                                                                                                            gcgcATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTaa                 >  2:306928/1‑141 (MQ=255)
                                                                                                                                 tgcatAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATAtt            >  1:292461/1‑141 (MQ=255)
                                                                                                                                     tAAAAGTCCCTAACAGCCTGAGGTCATTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAATGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTg        <  1:403237/141‑1 (MQ=255)
                                                                                                                                     tAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTg        >  1:261984/1‑141 (MQ=255)
                                                                                                                                     tAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGAGCTTTAGCGCATACCTCAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATGCTTg        >  1:92523/1‑141 (MQ=37)
                                                                                                                                      aaaaGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGa       <  2:626584/141‑1 (MQ=255)
                                                                                                                                      aaaaGCCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAATGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGa       <  2:659185/141‑1 (MQ=255)
                                                                                                                                      aaaaGCCCCTAACAACCTGAGGTCACTACGCTCCCGTCCGCCCCCTCTTTAGCTCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGa       <  2:300988/141‑1 (MQ=25)
                                                                                                                                        aaGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGAcc     <  2:392328/141‑1 (MQ=255)
                                                                                                                                           tCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCttt  <  1:225288/141‑1 (MQ=255)
                                                                                                                                           tCCCTAACAACCCGAGGTCCCTACGCTTCCGTTCCCCTGAGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCATATAGTATTTTTTACATTGATTTTAAATATTCTTGACCttt  <  2:244510/141‑1 (MQ=37)
                                                                                                                                           |                                                                                                                                            
TTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTACGCTTCGGTTCGCCTGCGCTTTAGCGCATGCATAAAAGTCCCTAACAACCTGAGGTCACTACGCTTCCGTTCGCCTGCGCTTTAGCGCATGCATAAAAGCCCCTAACAACCAAAGGTTGTAAGGGGCTTTAAATGTCAGATAGTATTTTTTAAATTGATTTTAAATATTCTTGACCTTT  >  CP000730/2504850‑2505129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: