Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,543,088 G→A 90.2% R283K (AGA→AAA)  hlgA → gamma‑hemolysin component A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,543,0880GA90.2% 157.8 / 7.9 62R283K (AGA→AAA) hlgAgamma‑hemolysin component A
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (24/31);  total (27/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

AACTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAATCATCTTAA  >  CP000730/2542950‑2543224
                                                                                                                                          |                                                                                                                                        
aaCTACCTCCTTTAACTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGaa                                                                                                                                        >  1:494266/1‑140 (MQ=255)
aaCTACCTCCTTTAAATCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGAc                                                                                                                                        >  1:494153/1‑141 (MQ=255)
  cTACCTCCTTTAATTCAAAGGGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACAt                                                                                                                                      >  1:191770/1‑141 (MQ=255)
          tttAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACGTGGATGCTACCTATGCTTACGTGACAAAACATCGTTTAGc                                                                                                                              <  1:584686/141‑1 (MQ=255)
           ttAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGcc                                                                                                                             <  2:543470/141‑1 (MQ=255)
                tCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGa                                                                                                                        <  1:529019/141‑1 (MQ=255)
                tCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAAAATCGTTTATCCGTTGa                                                                                                                        >  2:250371/1‑141 (MQ=255)
                 cAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACATGACATCGTTTAGCCGTTGAt                                                                                                                       <  1:405845/141‑1 (MQ=255)
                      tGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGaaa                                                                                                                  >  1:55298/1‑141 (MQ=255)
                       ggCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGaaaa                                                                                                                 >  1:341295/1‑141 (MQ=255)
                          tttAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACat                                                                                                              <  2:257230/141‑1 (MQ=255)
                                 catcatTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCtt                                                                                                       <  2:596684/141‑1 (MQ=255)
                                 catcatTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCtt                                                                                                       <  1:610315/141‑1 (MQ=255)
                                   tcatTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTAGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATGGTTTAGCCGTTGATAGAAAACATGATGCtttt                                                                                                     >  1:446327/1‑141 (MQ=255)
                                         attaCAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAAc                                                                                               <  1:432605/141‑1 (MQ=255)
                                           taCAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCg                                                                                             >  2:435600/1‑141 (MQ=255)
                                            acaacaTTGTCACACGAAAGAGGTAAAGGTGATAAAAGGGAGTGTGGAATCACTTACGGCAGAATCATGGATGCGTCATGTGCTTACGTGTCACCACATCGTTTAGCCGGTTATAGAAAACATTATGGTTTTAAAAACCGa                                                                                            <  2:191714/141‑1 (MQ=255)
                                            acaacaGTGTCCAACGAAATAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACCTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGa                                                                                            <  2:276403/141‑1 (MQ=255)
                                               acacTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAAc                                                                                         <  2:467809/141‑1 (MQ=255)
                                               acaTTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAAc                                                                                         >  1:97436/1‑141 (MQ=255)
                                                caTTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGaaaag                                                                                        >  1:74906/1‑139 (MQ=255)
                                                caTTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACg                                                                                        >  1:175619/1‑141 (MQ=255)
                                                   tGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTa                                                                                     <  2:159836/141‑1 (MQ=255)
                                                             aaGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAata                                                                           <  1:555264/141‑1 (MQ=255)
                                                             aaGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAata                                                                           <  1:386721/141‑1 (MQ=255)
                                                              agagGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAataa                                                                          >  2:202503/1‑140 (MQ=255)
                                                                agGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACCGTTAAATATGa                                                                        >  2:584556/1‑141 (MQ=255)
                                                                     aaGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTAACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGa                                                                   <  1:451317/141‑1 (MQ=255)
                                                                      aGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGaa                                                                  >  2:176154/1‑141 (MQ=255)
                                                                        gTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACt                                                                <  1:440023/141‑1 (MQ=255)
                                                                             aaagGCGAGTTTGAAATCACGTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGACATCGTGTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGaaa                                                           <  2:164062/141‑1 (MQ=255)
                                                                                aGCGAGTGTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTTCAGTGAAATATGAAGTGAACTGGAAAaca                                                        <  2:642336/141‑1 (MQ=255)
                                                                                   gAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACAt                                                     <  1:277365/141‑1 (MQ=255)
                                                                                      tttGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGaa                                                  >  1:35477/1‑141 (MQ=255)
                                                                                        tggaaTCACTTACGGCAGAAACATGGATGCTACCTATGCTTACGTGACCAAACATCGTTTAGCCGTTGATAGAAAACATGCTGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGt                                                <  1:115915/138‑1 (MQ=255)
                                                                                          aaaTCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTACCTAAAAATGTAACTGGAAAACACATGAAGTaa                                              <  1:660248/141‑1 (MQ=255)
                                                                                            aTCACTTCCTGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTaaaa                                            <  2:336557/141‑1 (MQ=255)
                                                                                            aTCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTaaaa                                            <  2:337772/141‑1 (MQ=255)
                                                                                            aTCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCAGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTaaaa                                            <  2:282137/141‑1 (MQ=255)
                                                                                            aTCACTTACCGCAGAAACATGGATGCTACATAGGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTaaaa                                            <  1:423719/141‑1 (MQ=255)
                                                                                             tCACTTACGTCAGAACCCTGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTaaaaa                                           <  1:118082/141‑1 (MQ=255)
                                                                                              tcgtttcGGCAGAAACATGGACGCTACTTATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAAt                                          <  1:308206/135‑1 (MQ=255)
                                                                                                cTTACGGCGGGAACATGGATGCTACATATGCCTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTa                                        <  2:262981/141‑1 (MQ=255)
                                                                                                 ttACGACAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTaa                                       <  1:293502/141‑1 (MQ=255)
                                                                                                   tcGGCAGAAACGTGGAGGCGACATAATCTTACGTGACAAAACATCGTTTAGCCGTTGACAGAAAACATGATGCTTGTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTaaaa                                     <  2:423085/140‑1 (MQ=255)
                                                                                                       cAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTTGAAAACACATGAAGTAAAAATTAAAAGCAt                                 <  2:97436/141‑1 (MQ=255)
                                                                                                            aCATGGATGCTACATATGCTTACGTGACAAAACAGCGTTTAGCAGTCTACATTAGACGTGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCacac                            <  1:313638/141‑1 (MQ=255)
                                                                                                              aTGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCt                          <  1:436517/141‑1 (MQ=255)
                                                                                                              aTGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCt                          <  2:341295/141‑1 (MQ=255)
                                                                                                              aTGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACAAATGAAGTAAAAATTAAAAGCATcacaaca                          >  2:12625/1‑138 (MQ=255)
                                                                                                                     cTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCtaaataaa                   >  2:3496/1‑141 (MQ=255)
                                                                                                                     cTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGaaaa                   >  2:121898/1‑141 (MQ=255)
                                                                                                                        cATATGCTTACGTGACAAGACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAg                >  2:160878/1‑141 (MQ=255)
                                                                                                                         atatGCATACGCGACACAACATCGTTGAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGt               <  2:423883/141‑1 (MQ=255)
                                                                                                                            tgattgcGTGCCAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAGAAAAAAATTAAAAGTATAACACCGAAGTAAACAGTTCa            >  2:242112/7‑141 (MQ=255)
                                                                                                                            tGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCa            >  2:221325/1‑141 (MQ=255)
                                                                                                                                 aCGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACAGGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAatcat       >  2:499546/1‑141 (MQ=255)
                                                                                                                                      aCAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAATCATCTTaa  >  1:304311/1‑141 (MQ=255)
                                                                                                                                      aCAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAATCATCTTaa  >  1:392707/1‑141 (MQ=255)
                                                                                                                                      aCAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATACCACCTAAGTAAACAGTTCAATCATCTTaa  >  1:642005/1‑141 (MQ=255)
                                                                                                                                      aCAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGATACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTACATCATCTTaa  >  2:10679/1‑141 (MQ=255)
                                                                                                                                      aCAAAACATAGTCTAGCCGTTGCCAGAAAACATCATGCTTTTAAGAACCGAAACTGTACCTTTAAATATTAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAATCATCTTaa  >  2:460201/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                        
AACTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAATCATCTTAA  >  CP000730/2542950‑2543224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: