Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 36.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT36.7% 162.7 / 93.4 110intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (42/24);  new base T (19/19);  total (64/46)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

Only 100 of 111 total aligned reads displayed.

ACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAA  >  CP000730/76053‑76320
                                                                                                                               |                                                                                                                                            
aCTAATATTGCTGCACCTGTATCAACTAGAAGCACTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGAAAAGTTGCATCTATAGTATAAttt                                                                                                                                 >  2:611076/1‑141 (MQ=255)
 cTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                                <  1:638190/141‑1 (MQ=255)
        tgctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGCATACTAACACAAAGGCAATTAATTTAATAACTTCAGGTAATGATAGGTTCTGTTGCAAAGTTGAATCTGTAGTATAATTTGAACgaaa                                                                                                                         >  1:19293/1‑141 (MQ=255)
           tgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTGGAATCTATAGTATAATTTTAACAAAAAgg                                                                                                                      >  1:532232/1‑141 (MQ=255)
           tgcACCTGTATCACATAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAgg                                                                                                                      >  2:452451/1‑141 (MQ=255)
             cACCTGTATCACTTAGAACAGCTAAACAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAg                                                                                                                    >  2:305044/1‑141 (MQ=255)
              aCCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGt                                                                                                                   >  1:144429/1‑141 (MQ=255)
                  gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                               <  1:297412/141‑1 (MQ=255)
                     tCACTTAGAACAGCTAACCAACGTGTTGGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATCGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTATGCTGt                                                                                                            >  2:207669/1‑141 (MQ=38)
                     tCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGt                                                                                                            >  2:127341/1‑141 (MQ=255)
                        cTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                         <  1:165281/141‑1 (MQ=255)
                        cTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAAAAAAAAGAAGtcttctaactg                                                                                                         >  1:163895/1‑136 (MQ=255)
                        cTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCCGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                         <  1:305658/141‑1 (MQ=255)
                         ttAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGa                                                                                                        <  2:334521/141‑1 (MQ=255)
                             aaCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTa                                                                                                    <  1:254019/141‑1 (MQ=255)
                             aaCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTa                                                                                                    <  1:656314/141‑1 (MQ=255)
                                 gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAGAGGCAATCAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACATAACGGAGGCTTCTGTATTAATTAATTc                                                                                                >  2:416190/1‑141 (MQ=37)
                                 gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                >  1:295995/1‑141 (MQ=255)
                                    aaCAAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGa                                                                                             >  2:243282/1‑141 (MQ=255)
                                      ccAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCACAATATAGGTTCTGTTGCAGAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAtata                                                                                           >  1:49034/1‑141 (MQ=255)
                                           gtgtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAAc                                                                                      >  1:606153/1‑141 (MQ=255)
                                             gtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACaa                                                                                    >  1:468816/1‑141 (MQ=255)
                                             gtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACaa                                                                                    >  1:533434/1‑141 (MQ=255)
                                                 gTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAACAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTa                                                                                >  2:601696/1‑141 (MQ=255)
                                                       cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCTGATATAAACAATTTAACAAgg                                                                          >  1:515676/1‑141 (MQ=255)
                                                         gggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATGGGAGATATAGCAAAGACGATTCTGTAGTATAATTTTAACAAAATGGAAGCTTGTTTATGAACTATTTCAGATATAAGCAATTTAACAAgggt                                                                        >  1:318930/1‑139 (MQ=21)
                                                           gAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGt                                                                      <  2:632965/141‑1 (MQ=255)
                                                                cTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTGTGTGGCAGAGTTGAATCGATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAAAAGGATGTTATCa                                                                 >  2:303566/1‑141 (MQ=21)
                                                                   acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                              <  2:42836/141‑1 (MQ=255)
                                                                     acaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                            <  1:496495/141‑1 (MQ=255)
                                                                            cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTg                                                     >  2:478850/1‑141 (MQ=255)
                                                                               ttaatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                  <  1:461822/141‑1 (MQ=255)
                                                                                 aatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctac                                                <  2:295995/141‑1 (MQ=255)
                                                                                  atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                               <  1:243282/141‑1 (MQ=255)
                                                                                  atttaatAATTATAGCAAAATATAGGTTCTGCTGCAAAGTTGAATATATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGTACTATTTCATATATAAACAATTTAACAAGCACGTTATCACTGTAGCCGTTGGctact                                               >  2:304280/1‑141 (MQ=17)
                                                                                    ttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTAt                                             >  2:248537/1‑141 (MQ=255)
                                                                                       ataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTa                                          >  1:521542/1‑141 (MQ=255)
                                                                                        taatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTaa                                         >  1:223289/1‑141 (MQ=255)
                                                                                        taatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTaa                                         >  2:68613/1‑141 (MQ=255)
                                                                                        taatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTaa                                         >  1:213091/1‑141 (MQ=255)
                                                                                        aaatTATAGCAAAATATAGGTTCCGTTGCAAAGTTGAATCTATAGTATAATTTTAAGAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTaa                                         <  1:7047/140‑1 (MQ=255)
                                                                                         aatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGCAGCCGTGGGCTACTATCTAAg                                        <  2:144429/141‑1 (MQ=255)
                                                                                            tataGCAAAATATAGGTTCTGTAGCAAAGTTGAATCTAAAGCATAATTTTAACAAAAAGGAGTCGTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTCCTACCTAAGata                                     >  2:25821/1‑141 (MQ=14)
                                                                                                gCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                 >  1:506245/1‑141 (MQ=255)
                                                                                                gCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                 >  1:636328/1‑141 (MQ=255)
                                                                                                gCAAAATATAGGTTATGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                 >  1:347599/1‑141 (MQ=255)
                                                                                                 tgtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTCCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                                <  1:305151/133‑1 (MQ=11)
                                                                                                 cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                >  1:414361/9‑141 (MQ=1)
                                                                                                 cagttaggaGGTTCTGTGGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                <  1:141184/133‑1 (MQ=11)
                                                                                                 agtaaatgaGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                                >  2:124922/9‑141 (MQ=255)
                                                                                                  gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                               >  1:335998/8‑141 (MQ=1)
                                                                                                  gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                               >  1:273629/8‑141 (MQ=0)
                                                                                                  agttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtt                               >  1:440436/8‑141 (MQ=2)
                                                                                                   taaatgaGGTTCTGTTGCATAGTTGAATTTATAGTAAACTTTTAACAAACAGGGTTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACGGTAGCGGTTGGCTACTATCTAAGATATGCATTg                              <  2:158101/135‑1 (MQ=11)
                                                                                                   gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAttt                              >  1:468732/7‑140 (MQ=1)
                                                                                                   gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                              <  1:530788/135‑1 (MQ=2)
                                                                                                   aaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                              >  2:156245/1‑141 (MQ=255)
                                                                                                    ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                             >  1:363896/6‑141 (MQ=2)
                                                                                                    ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                             >  2:559875/6‑141 (MQ=0)
                                                                                                    aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAGGGCTGTTATCACTGTAGCGGTTGGCTACTATCTAAGATATGCATTgg                             >  2:566319/6‑140 (MQ=11)
                                                                                                    aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                               >  2:76112/6‑139 (MQ=0)
                                                                                                    aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                               <  1:76112/134‑1 (MQ=255)
                                                                                                    aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTgg                             >  1:390817/6‑140 (MQ=255)
                                                                                                    aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                             <  1:142294/136‑1 (MQ=9)
                                                                                                    aaatatgattcatttGCAAAGTTATATTTATTATAAAATAAAAAAAAAAAGGAGTCTTCTGTATTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                             <  2:282395/128‑1 (MQ=11)
                                                                                                     taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                            <  1:237188/137‑1 (MQ=255)
                                                                                                     atatAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                            >  2:524550/1‑141 (MQ=38)
                                                                                                     aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            <  1:190529/137‑1 (MQ=2)
                                                                                                     aatgaGGTTCTGTTCCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTTTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            <  1:475144/137‑1 (MQ=11)
                                                                                                      tttaGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGt                           <  1:325616/139‑1 (MQ=37)
                                                                                                      atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                           <  1:562958/138‑1 (MQ=9)
                                                                                                      atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                           <  2:533211/138‑1 (MQ=2)
                                                                                                      aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                           <  2:363401/138‑2 (MQ=9)
                                                                                                      aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                           <  2:204467/138‑2 (MQ=11)
                                                                                                        gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                         >  1:124396/2‑141 (MQ=2)
                                                                                                        gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                         >  2:249782/2‑141 (MQ=11)
                                                                                                        gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                         >  2:43752/2‑141 (MQ=0)
                                                                                                        gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGATATGCATTGAgcta                         <  2:414361/140‑4 (MQ=11)
                                                                                                         aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAATAATAATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                        >  1:516632/1‑141 (MQ=11)
                                                                                                         aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGTTGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                        >  1:148603/1‑141 (MQ=2)
                                                                                                         aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTCTATAAAGTATGCATTGAGTTAt                        >  1:594719/1‑141 (MQ=11)
                                                                                                         aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                        >  2:613755/1‑141 (MQ=11)
                                                                                                         aGGTTCTGATGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTACTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATAACTGTAGCCGTTGACTACTATCTAAGATATGCATTGAGCTAt                        >  2:323256/1‑141 (MQ=1)
                                                                                                          ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                       >  2:57876/1‑141 (MQ=34)
                                                                                                          ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                       >  1:555987/1‑141 (MQ=35)
                                                                                                          ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                       >  1:563434/1‑141 (MQ=35)
                                                                                                          ggTTCTGTAGCGAAGTTGAATATATAATATAATTTTACCAAAAATGAGTCGTCTGTATGCACGCTTTCAGATATAAACAATTTAACTAGGATGTTATCACTGTAGCCTTTGGCTACTATCTAAGATATGCATTGAGCTATc                       <  2:297860/141‑1 (MQ=11)
                                                                                                           gttccgcagaaacgTTGAATGTATAGTATAATATTGACAACACGGCGTCTTCTCTTCGATGGAGTTGAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCg                      >  2:469793/14‑141 (MQ=2)
                                                                                                            ttttGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                     <  2:282845/138‑1 (MQ=2)
                                                                                                                gTTGCAAAGTTGAATCTATAGTATAATTTTAACTAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGGGata                 <  1:452451/141‑1 (MQ=25)
                                                                                                                actggaaaGTTGAATTTATAATAAAAAAATAACAAAAAGGAGTCTTCTGTAGGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                 <  2:329564/136‑1 (MQ=1)
                                                                                                                    gaaggTTGAATGTATGGTATACTTGTAACAAAAAGGAGGCTTCGGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCGAAGATATGCATTGAGTTATCGTGatatagc             <  2:264603/137‑3 (MQ=11)
                                                                                                                    cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc             <  1:478850/141‑1 (MQ=35)
                                                                                                                     aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCt            <  1:127341/141‑1 (MQ=35)
                                                                                                                     aaaGTGGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGTTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCt            <  1:207669/141‑1 (MQ=21)
                                                                                                                       aGTTGAATGTATAGAATAATTTTAACAAAAAGGAGTCTTCTGTATAAAACATTTCAGATATAAACAATTTAACAAGGATGTTATCACTTTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa          <  1:222137/141‑1 (MQ=11)
                                                                                                                       aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa          <  1:214923/141‑1 (MQ=35)
                                                                                                                        gTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTGTCATGATATAACTGaa         >  1:619514/1‑141 (MQ=255)
                                                                                                                          tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat       >  1:6207/1‑141 (MQ=35)
                                                                                                                          tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCATGATATATCTGaaaa       >  2:120017/1‑140 (MQ=25)
                                                                                                                               |                                                                                                                                            
ACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAA  >  CP000730/76053‑76320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: