Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 58.4% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC58.4% 369.2 / inf 778intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (172/151);  new base C (232/220);  total (406/372)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 781 total aligned reads displayed.

TAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAA  >  CP000730/76157‑76429
                                                                                                                                          |                                                                                                                                      
  ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                                                                                                                                    >  1:555987/1‑141 (MQ=35)
        gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                                                                                                                              <  1:199477/141‑1 (MQ=35)
               aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa                                                                                                                       <  1:214923/141‑1 (MQ=35)
                       ttattGTAAAATTTGAACAAAAAGGAGTCTTCTGTATTAACTATTTCAGATCTAATCAATCTAACAAGGAAGTTATCACTAGAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa                                                                                                               <  2:170984/140‑1 (MQ=11)
                       ttataGTATAATTTTAACAAAAAGGAGTCTTCTGGATGAACTATTACAGGTATAGACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa                                                                                                               <  1:313795/140‑1 (MQ=11)
                        tataGTATAATTTTGACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCAGTTGCTTGCTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg                                                                                                              >  1:348163/1‑141 (MQ=11)
                         ataatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                                             <  1:146962/137‑2 (MQ=12)
                         ataaaataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTGGGCTACCATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                                             <  2:434365/136‑2 (MQ=11)
                           aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                                           >  2:563384/3‑141 (MQ=12)
                           aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                                           <  1:491116/141‑1 (MQ=17)
                           aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                                           <  1:59446/141‑1 (MQ=17)
                              ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                                        <  2:161149/141‑1 (MQ=17)
                              ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                                        <  1:268299/141‑1 (MQ=12)
                               taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgt                                                                                                       >  2:36529/1‑141 (MQ=17)
                                    ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGgtgt                                                                                                  <  2:288319/141‑1 (MQ=17)
                                     tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAATGTGGTGTa                                                                                                 >  2:199048/1‑141 (MQ=12)
                                     tAACAAAAAGGAGTCTGCTGTATGAACTATTTCAGATATAAACATTTTAACTAGGATGTTATCACTGTAGCTTTTGGCTACTATCTAATATAGGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                                                                                 <  2:139379/141‑1 (MQ=11)
                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                >  2:8715/1‑141 (MQ=17)
                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATCAAGAGAACGTGGTGTaa                                                                                                >  1:85721/1‑141 (MQ=12)
                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAGTTAACAGGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                >  2:388577/1‑141 (MQ=255)
                                       aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                                               >  1:499062/1‑141 (MQ=17)
                                       aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTCGGCTACTATCTAAGACATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                                               <  1:642534/141‑1 (MQ=255)
                                           aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtt                                                                                           >  2:76858/1‑141 (MQ=17)
                                            aacgggtctgtggTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                          <  2:298674/129‑1 (MQ=11)
                                               gtttcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                                                       <  2:19230/138‑1 (MQ=12)
                                               gattcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                                                       <  1:364681/138‑1 (MQ=12)
                                               gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                                                       <  2:486690/141‑1 (MQ=17)
                                                aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                                                      <  2:600315/141‑1 (MQ=17)
                                                 gTCTTCTGAAAGAACTATTTCAGAGATAACCAATATAAGACGGTGGTTATCACTGTAGCCGTTGGCTACAATGTAAGATATGTATTGAGCTATCGTGCTATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                                                     <  2:459742/141‑1 (MQ=11)
                                                  tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                                                    >  2:218522/1‑141 (MQ=17)
                                                   cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTc                                                                                   <  2:259035/141‑1 (MQ=12)
                                                       tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATAGCTGAAATATTCAGTGAAAGAGCGGTAAACGTTCATCATTCACCg                                                                               >  2:631667/1‑141 (MQ=11)
                                                           tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt                                                                           >  1:53689/1‑141 (MQ=17)
                                                              aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                        >  1:125470/1‑141 (MQ=12)
                                                               cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCg                                                                       >  1:290110/1‑141 (MQ=12)
                                                                tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCAGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                      >  1:627783/1‑141 (MQ=255)
                                                                 aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                     >  1:412699/1‑141 (MQ=12)
                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                                                    >  2:238599/1‑141 (MQ=17)
                                                                    tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                  <  2:352804/141‑1 (MQ=17)
                                                                    tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                  >  1:607071/1‑141 (MQ=17)
                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGGACGTGGAGTAAAAGTTCATCATTCAACGGTATACCGTTGGGt                                                                 >  2:328679/1‑141 (MQ=11)
                                                                      aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                >  2:377454/1‑141 (MQ=255)
                                                                        atatAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                              <  2:12999/141‑1 (MQ=17)
                                                                          ataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTAAACGATCTAACGTTGGGTTCAAg                                                            >  2:409274/1‑141 (MQ=11)
                                                                           taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                           >  2:314775/1‑141 (MQ=17)
                                                                           taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTAAACGGTCTACCGTTGGGTTCAAGa                                                           >  2:269841/1‑141 (MQ=17)
                                                                           taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                           >  2:208436/1‑141 (MQ=255)
                                                                             aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                         <  1:190093/141‑1 (MQ=255)
                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGAGTTCAAGAata                                                        >  2:47679/1‑141 (MQ=255)
                                                                               cAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAatat                                                       >  2:423579/1‑141 (MQ=17)
                                                                               cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                       <  2:508707/141‑1 (MQ=12)
                                                                                  tttAAAAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                    <  1:48739/141‑1 (MQ=17)
                                                                                   ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGCACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                   >  1:72372/1‑141 (MQ=17)
                                                                                   ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCCACGCTCTACCGTTGGGTTCAATAATATGccc                                                   <  2:321862/141‑1 (MQ=255)
                                                                                   ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGccc                                                   <  1:256571/141‑1 (MQ=255)
                                                                                     aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                 >  1:44683/1‑141 (MQ=17)
                                                                                     aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                 >  1:117608/1‑141 (MQ=17)
                                                                                     aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATAGTGATAGATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                 >  1:92690/1‑141 (MQ=11)
                                                                                      aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccccca                                                >  2:18013/1‑139 (MQ=17)
                                                                                      aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                                                >  2:526883/1‑141 (MQ=17)
                                                                                      aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTAATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                >  2:151597/1‑141 (MQ=255)
                                                                                           gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATAGCTGAAATATTATGAGAACGTGGTGTAGACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttttt                                           >  2:499288/1‑140 (MQ=11)
                                                                                            gtGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATTGTGATATATCTGAAATATTAAGAGACCGTGGTGTAAACTTTCATCATTCAACGATCGACCGTTGGGTTCAAGAATATTCCCCAATTTtat                                          <  2:249331/140‑1 (MQ=11)
                                                                                            aTGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCCATTTtat                                          >  1:522034/1‑141 (MQ=18)
                                                                                            aTGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                          <  2:231860/141‑1 (MQ=18)
                                                                                             tGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAGCGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                         <  1:430312/141‑1 (MQ=21)
                                                                                             tGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAACATGCCCCAATTTtata                                         <  1:394707/141‑1 (MQ=25)
                                                                                               ttATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                       <  1:163002/141‑1 (MQ=25)
                                                                                               ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATAGGCCCCAATTTTatata                                       >  1:173058/1‑140 (MQ=255)
                                                                                                 aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCAATGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGCATATGCCCCAATTTTATATCaa                                     >  1:460965/1‑141 (MQ=11)
                                                                                                  tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                    >  1:659085/1‑141 (MQ=255)
                                                                                                   cattGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                   <  1:222672/138‑1 (MQ=25)
                                                                                                     tagtagccaacggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt                                 >  2:569468/12‑141 (MQ=11)
                                                                                                     cTGTAGCTGTTGGCTAATATCTAAGATATGTATTCAGTTCTAGTGATATATCAGCAATATTATTCTAATGAGATGTAAACGATCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt                                 >  1:100896/1‑141 (MQ=11)
                                                                                                      tGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                <  1:301589/141‑1 (MQ=255)
                                                                                                      tGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                <  1:3842/141‑1 (MQ=255)
                                                                                                       gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                               >  2:614173/1‑141 (MQ=25)
                                                                                                       gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                               <  1:327189/141‑1 (MQ=25)
                                                                                                        tAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaaaaaaaa                              <  2:647959/141‑7 (MQ=255)
                                                                                                        aaGCCGTTGGCTACTACCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAACCGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                              <  1:280349/140‑1 (MQ=11)
                                                                                                          gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                            >  1:48077/4‑141 (MQ=25)
                                                                                                           ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATCTATCTGCAATATTAAGAGCACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGACCCAATTTTATATCCAATTTGgaaga                           >  2:115730/1‑141 (MQ=11)
                                                                                                           ccGTTGGCTACTATCTAAGATATGCATGGGGCTATCGTGATATATCGGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                           >  2:659908/1‑141 (MQ=11)
                                                                                                               tcgCTAATATGTAAGATATGCATTGAGCTATCGGGATATAGCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                       <  1:547592/139‑1 (MQ=11)
                                                                                                                ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                      <  1:563729/141‑1 (MQ=255)
                                                                                                                ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                      <  1:356866/141‑1 (MQ=255)
                                                                                                                    actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                  >  1:244905/1‑141 (MQ=255)
                                                                                                                     ctaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                 <  2:587567/141‑1 (MQ=35)
                                                                                                                      taTCTAACATATGCCTTGAGCTACCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                <  1:135747/141‑1 (MQ=11)
                                                                                                                        ccTAAGATAGGCATTGAGCCATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa              <  1:232316/140‑1 (MQ=11)
                                                                                                                          tAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATacaaaaa            >  2:554349/1‑139 (MQ=11)
                                                                                                                            aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTACACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGACGAAAAAGCATaaaaaagct          >  1:583231/1‑137 (MQ=9)
                                                                                                                            aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct          >  2:546391/1‑137 (MQ=17)
                                                                                                                            aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCAACGATCTACCGTTGGGTTCCAGAATAGGCCCCAATTTTAAATCAAATTGGGACGGAAAAGCACaaaaaatct          >  2:445256/1‑137 (MQ=11)
                                                                                                                              atatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta        >  2:101608/1‑135 (MQ=14)
                                                                                                                              atatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGGTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta        >  2:280720/1‑135 (MQ=2)
                                                                                                                              atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCCAATTTGGAAGAAAAAGCATaaaaaagctta        >  1:336534/1‑135 (MQ=11)
                                                                                                                               tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAACAgacttat       >  2:579925/1‑135 (MQ=255)
                                                                                                                               tatGCATTGAGCCATCGTGATATATCTGAAATATTAAGAGAACGTGGCGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat       <  2:339873/141‑8 (MQ=11)
                                                                                                                               tatGCATTAAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGCCAACGTTCATCATTCACCCGTCTACCGTTGGGTTCAATAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAAtt       <  2:468816/141‑1 (MQ=14)
                                                                                                                                          |                                                                                                                                      
TAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAA  >  CP000730/76157‑76429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: