Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,353 G→A 55.7% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3530GA55.7% 149.3 / inf 564intergenic (+411/‑723)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (133/114);  new base A (165/143);  total (302/262)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-02

Only 100 of 572 total aligned reads displayed.

GTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATT  >  CP000730/76213‑76492
                                                                                                                                            |                                                                                                                                            
gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTTAAACTGTTGTCTACTATCTAAGATATGCATTGAGTTAGCGTTATATCTCTGAAATATGAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                                                                                              <  2:566916/141‑1 (MQ=11)
  aTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTTGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCTTTCAACGGTc                                                                                                                                            <  1:482826/141‑1 (MQ=12)
   tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                                                                                           >  2:120386/1‑141 (MQ=12)
    gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGTGTTTTCGTGATATATCTGATATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTa                                                                                                                                          <  2:273629/141‑1 (MQ=255)
    gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCtg                                                                                                                                          >  1:401167/1‑140 (MQ=17)
     aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                                                         >  2:350771/1‑141 (MQ=12)
      aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                        <  2:32334/141‑1 (MQ=12)
      aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                        >  1:125470/1‑141 (MQ=12)
        tctttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                                                      <  2:577639/139‑1 (MQ=255)
          tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTGTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAACCGTTCATCATTCAACGATCTACCGTTg                                                                                                                                    >  1:298194/1‑141 (MQ=11)
          tctCAGATATAAACAATTTAACAAGGATGTTATCACGGTAGCTGTTGGCTACTATCTAAGAGATGCATTGAGTTATCTTAATATATCTGAAATATTAAGGTAACGTTGTGTAAACGTTCATCATGCAACGGTCTACCGTTg                                                                                                                                    <  2:190670/139‑1 (MQ=11)
            tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                  >  2:296806/1‑141 (MQ=12)
            tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                  <  2:84082/141‑1 (MQ=255)
             cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                                                 >  2:5960/1‑141 (MQ=12)
              aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATGTTAACGGAACGAGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                                                                                >  1:287262/1‑141 (MQ=255)
              aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAAGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                                                                                >  2:234134/1‑141 (MQ=17)
              aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGATATCGTGATATATTTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                                                                                >  2:386545/1‑141 (MQ=255)
                atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGACCTACCGTTGGGTTCa                                                                                                                              >  1:384992/1‑141 (MQ=255)
                  aacaatatattAACAAGGATGTTATCACTGCAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                                                                            <  2:136447/132‑1 (MQ=11)
                   taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                           >  2:208436/1‑141 (MQ=255)
                    aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGaa                                                                                                                          <  1:143567/141‑1 (MQ=17)
                     aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTTCCGTTGGGTTCAAGAat                                                                                                                         >  2:612295/1‑141 (MQ=255)
                     aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                                                                                         >  1:353820/1‑141 (MQ=12)
                      aCAATTTAATAAGGATGTTATCACTGTAGCAGTTGTGCGCCAGCTAAGAAATGCATGAAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGaaaa                                                                                                                        >  1:487548/1‑139 (MQ=11)
                      aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAACGTTGGGTTCAAGAata                                                                                                                        >  2:286236/1‑141 (MQ=17)
                       cgaTTTAACAAGGATGGTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                                                       <  2:421581/139‑1 (MQ=11)
                       cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                                                       >  2:530724/1‑141 (MQ=12)
                        aaTTTAACAAGGATTTTATCACTGTAGCCGTTGGCTATTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                                                      <  2:370152/141‑1 (MQ=11)
                        aaTTTAACAAGGATGTTATAACTGTAGATGTTGACTACTAACTACGATATGGGTGGAGTAGTCGCAGTATATCTAAAAGATTAAGGTTACGTGGGGTAAAGGATCATCGTTTAACGGTCTACCGTTGGGTTAAAAAataag                                                                                                                      >  2:157824/1‑139 (MQ=2)
                         aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                                                     <  1:276721/141‑1 (MQ=32)
                         aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                                                                                                                     >  2:242182/1‑141 (MQ=255)
                          tttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                                                                                    <  2:508437/141‑1 (MQ=17)
                          tttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                                                                                    <  2:150746/141‑1 (MQ=17)
                          tttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATAGATCTGAAATATTAAGGGAACGGGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                                                                                    <  2:72517/141‑1 (MQ=12)
                           ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGGATATGccc                                                                                                                   >  2:463851/1‑141 (MQ=255)
                             aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                                                 >  1:164115/1‑141 (MQ=17)
                             aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                                                 >  1:93876/1‑141 (MQ=255)
                              aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAACTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                                >  2:473646/1‑141 (MQ=255)
                               cAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                                                                               >  1:640916/1‑141 (MQ=17)
                                aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                                                                                              >  1:595437/1‑141 (MQ=255)
                                 aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                             <  2:331831/141‑1 (MQ=255)
                                  ggATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCACCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                                                                            <  2:498189/141‑1 (MQ=17)
                                  ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCACtttt                                                                                                            >  2:655512/1‑141 (MQ=11)
                                     tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                         >  2:164051/1‑141 (MQ=255)
                                     tGTAATCACTGTAGCTGTTGGCTACTATCCAACATAAGCATCCAGTTAACGTGATATATCTGAAATATTAAGGGAACCTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                         <  1:89007/141‑1 (MQ=11)
                                       ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                                                       <  1:583946/141‑1 (MQ=255)
                                       ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATAGGCCCCAATTTTatata                                                                                                       >  1:173058/1‑140 (MQ=255)
                                        tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTCTAGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                      >  1:456627/1‑141 (MQ=25)
                                        tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                                                      <  2:31916/141‑1 (MQ=255)
                                         aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATACCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                                                     >  1:444016/1‑141 (MQ=25)
                                         aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                                                     <  1:76858/141‑1 (MQ=255)
                                          tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                                                    >  1:659089/1‑141 (MQ=255)
                                           cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATTTCAAAt                                                                                                   >  1:30367/1‑141 (MQ=255)
                                            aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt                                                                                                  >  2:105531/1‑141 (MQ=35)
                                             cTGTAGCTGTTGGCTACTATCTAAGATATGCGTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGATTATGCCCCAATTGTATATCAAAttt                                                                                                 >  2:171760/1‑141 (MQ=255)
                                             cTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt                                                                                                 <  1:213780/141‑1 (MQ=25)
                                             cTGTAGCTGTTGGCTAATATCTAAGATATGTATTCAGTTCTAGTGATATATCAGCAATATTATTCTAATGAGATGTAAACGATCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt                                                                                                 >  1:100896/1‑141 (MQ=11)
                                              tGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                                                                                <  2:582988/141‑1 (MQ=255)
                                              tGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                                                                                <  1:250533/141‑1 (MQ=255)
                                              tGTACCTGTTGGCTACTATCTAAGATATGCATTTAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                                                                                <  1:472058/141‑1 (MQ=255)
                                               gTAGCTGTTGGCTACTATCTAAGATAGGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                               <  2:441513/141‑1 (MQ=25)
                                                tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                              >  2:361806/1‑141 (MQ=255)
                                                  gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                            >  1:536456/4‑141 (MQ=25)
                                                  gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGCTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTGGgaag                                                                                            >  2:221874/1‑141 (MQ=255)
                                                  gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTCATATCAAATTTGgaag                                                                                            <  1:332048/141‑1 (MQ=255)
                                                     gTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                                                                                         <  2:429248/141‑1 (MQ=255)
                                                     gTTGGCTACTATCTAAGATATGCATTAAGCTATCGTGATATATCTGAAATATTCCGCGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGCAGaaa                                                                                         >  1:19415/1‑141 (MQ=11)
                                                      ttGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaa                                                                                        <  1:559627/141‑1 (MQ=255)
                                                       tcgCTAATATGTAAGATATGCATTGAGCTATCGGGATATAGCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                                                                                       <  1:547592/139‑1 (MQ=11)
                                                       tGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                                                                                       >  1:111523/1‑141 (MQ=255)
                                                        ggttactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTCAGCGGACGTGGCGTAACCGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAACTTTACATAAAATTGGGAAGAAAAAg                                                                                      <  2:308270/138‑1 (MQ=11)
                                                        ggCTACTATCTAAGATATGCATTGCGCTATCGTGATATATCTGAAATATTAAGAGAACTTGGTTTAAACGTTCATCATTCAACGATCTACCGTTTGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                                                      <  2:487548/141‑1 (MQ=11)
                                                          caactaTCTAAGAAATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTAGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                    <  2:184038/139‑1 (MQ=11)
                                                           tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                   <  1:429766/141‑1 (MQ=255)
                                                           tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                   <  1:605731/141‑1 (MQ=255)
                                                            actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                                 <  1:627078/141‑1 (MQ=255)
                                                            actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                                 >  1:244905/1‑141 (MQ=255)
                                                             ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                                <  1:264436/141‑1 (MQ=255)
                                                             ataTCTAATAAATGCATTGAGCTATCGTGATATATTTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATACCAAATTTGGAAGAAAAAGCATaa                                                                                <  1:337990/140‑1 (MQ=11)
                                                              tttCTAAGTTATGCATTGTGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAATAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                               <  1:57855/139‑1 (MQ=11)
                                                              taTCTAAGGTATGCAGTGAGCGATCGGGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                               <  2:249572/141‑1 (MQ=11)
                                                              taTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                               <  1:223558/141‑1 (MQ=255)
                                                              taTCTAACATATGCCTTGAGCTACCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                               <  1:135747/141‑1 (MQ=11)
                                                                tCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                                                                             <  1:32381/141‑1 (MQ=35)
                                                                  tAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATacaaaaa                                                                           >  2:554349/1‑139 (MQ=11)
                                                                    aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                         >  2:546391/1‑137 (MQ=17)
                                                                    aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                         >  1:52231/1‑137 (MQ=11)
                                                                    aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                         >  2:263967/1‑137 (MQ=11)
                                                                      atatGCATTGAGTTATCGTGATATATCGGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctta                                                                       <  1:232914/141‑7 (MQ=2)
                                                                       tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGACTATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcgtat                                                                      >  2:56302/1‑134 (MQ=12)
                                                                       tatGCATTGAGTTAGCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                                                      >  1:367078/1‑134 (MQ=2)
                                                                       tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAACAAGCATAAAAAAGCTAAt                                                                       >  2:390331/1‑141 (MQ=11)
                                                                             ttGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAAGCTAATTACaaa                                                                 >  2:647994/1‑141 (MQ=1)
                                                                                           tatatCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATgag                                                  >  1:360982/1‑141 (MQ=40)
                                                                                              atCTGAAATATTAAGCGAACGTGGTATAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACGAATGGCGTATTGCTGAGACg                                               <  1:416190/141‑1 (MQ=255)
                                                                                                              gAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATGACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAg                               >  1:182772/1‑141 (MQ=38)
                                                                                                                  gtggtgTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTTGTGGGACGGACATCAAAATAAAAGGaaa                           >  2:636957/1‑141 (MQ=255)
                                                                                                                    ggtgTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAAt                         >  2:589729/1‑141 (MQ=40)
                                                                                                                            cGTCCATCATGCAACGGTCTACCGTTGGGTTCAAGAATAGGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGATACGTACATCAAAATAAAAGGAAAATGGAGCTAt                 <  1:309104/141‑1 (MQ=255)
                                                                                                                                           ggTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCAtt  >  1:504854/1‑141 (MQ=39)
                                                                                                                                            |                                                                                                                                            
GTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATT  >  CP000730/76213‑76492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: