Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,499,376 C→A 90.3% G883V (GGT→GTT)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,499,3760CA90.3% 152.6 / 9.2 64G883V (GGT→GTT) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (32/24);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

TCGCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGTTTGAT  >  CP000730/1499238‑1499514
                                                                                                                                           |                                                                                                                                          
tcgCCTCGATTGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACt                                                                                                                                          <  2:221571/141‑1 (MQ=255)
tcgCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCGTCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACt                                                                                                                                          >  1:333213/1‑141 (MQ=255)
     tCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAgg                                                                                                                                     <  1:623885/141‑1 (MQ=255)
      agATAGTATTTGGCATTTATTCCATCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggt                                                                                                                                    <  1:617938/140‑1 (MQ=255)
        aTAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtgg                                                                                                                                  >  1:479507/1‑141 (MQ=255)
            tATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATg                                                                                                                              >  1:602092/1‑141 (MQ=255)
            tATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGAATACCATGCCTAATAATAAACTACTGCTCCAATTTTTTTAACTTCAGGTGGTATg                                                                                                                              >  1:313145/1‑141 (MQ=255)
                 ggCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATaa                                                                                                                         <  2:213635/141‑2 (MQ=255)
                   cATTTACTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATacaa                                                                                                                       <  1:536325/141‑1 (MQ=255)
                        aTTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATaa                                                                                                                  >  2:290230/1‑141 (MQ=255)
                         ttCCAACAGGATTATTGATTGTTGGTGTAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAGACTACTGCTCTAATTTGTTTACCTCAAAGTGGTATGAATACCACATAAt                                                                                                                 >  2:63797/1‑141 (MQ=255)
                           tcAAGAGGATTGTTGATTGTTGGTGGAATGTCATCTGGCAAAATTGTAACAGCATTTCTCGTATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCt                                                                                                               <  1:509326/140‑1 (MQ=255)
                           ccAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACACAGTAATCt                                                                                                               >  2:105191/1‑141 (MQ=255)
                            cAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCtt                                                                                                              <  1:474629/141‑1 (MQ=255)
                             aaCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTg                                                                                                             >  2:288450/1‑141 (MQ=255)
                             aaCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTACTCTAATTTGTTTAACTACAGGTGGTATGAATATAAAATAATCtat                                                                                                             >  2:87745/1‑139 (MQ=255)
                              aCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGc                                                                                                            >  1:361020/1‑141 (MQ=255)
                                      attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGtct                                                                                                    >  1:664992/1‑139 (MQ=255)
                                           ttgttgGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAAACTAATCNTGCGTGCGTATAGTaa                                                                                               >  1:597071/1‑141 (MQ=255)
                                             ttgggtcgAAGGTCATCTTGTACAAGTGTAACAGCATTTCACATGTTAGATGCAACTCCTGTATTGTATTAAGCTGTTTCTCGAGTTAGTTTATCTACAGGTGGTATGAATACAGCTTCAGCGTGCCTTCATATCGTAAtt                                                                                             <  2:75109/138‑1 (MQ=255)
                                                ggtggAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCTACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATATTAATTGta                                                                                          >  1:440603/1‑141 (MQ=255)
                                                ggtggAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTAATGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTgtagtagagggt                                                                                          >  1:23751/1‑135 (MQ=255)
                                                  gggAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGtatt                                                                                        <  2:260710/140‑1 (MQ=255)
                                                      aTGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGc                                                                                    <  2:49939/141‑1 (MQ=255)
                                                       tggtaTCTGGCAACATAGGAACAGCATGACCCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAGTTTGGTTACCTACAGGTGGTATTAATACAACATAATCTTGCGGTCGTATAGTAATTGTACTTTGCa                                                                                   <  2:12719/137‑1 (MQ=255)
                                                       tGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCa                                                                                   >  2:135719/1‑141 (MQ=255)
                                                       tGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCa                                                                                   >  2:127655/1‑141 (MQ=255)
                                                         taaTCTGGACAAATATTAACCTCTTTACCAATATTAGATGCAACTACTTTATTATAACAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCAcc                                                                                 <  1:87745/139‑1 (MQ=255)
                                                         tCATCTGTCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCAcc                                                                                 <  1:202236/141‑1 (MQ=255)
                                                         tCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCAcc                                                                                 <  1:333265/141‑1 (MQ=255)
                                                         tCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCAcc                                                                                 <  1:288450/141‑1 (MQ=255)
                                                          cATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCAacc                                                                                >  1:302491/1‑141 (MQ=255)
                                                            tCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCa                                                                              >  1:517303/1‑141 (MQ=255)
                                                             ttGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTGTAGTAATTGTATTATGCACCCCAt                                                                             <  2:647525/140‑1 (MQ=255)
                                                             cTGGCAAAATAGTAACAGCATTACTCATATTAGATGAAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATCGTAATTGTATTATGCACCCCAt                                                                             >  1:461209/1‑141 (MQ=255)
                                                               ggCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTCATTGTATTATGCACCCCATCa                                                                           >  2:31884/1‑141 (MQ=255)
                                                                    aaTAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGgtgt                                                                      <  1:644736/141‑1 (MQ=255)
                                                                           aCAGCATTACTCATATTAGATGCAACTACTTTATTGTAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTc                                                               >  1:517150/1‑141 (MQ=255)
                                                                           aCAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTc                                                               >  1:265168/1‑141 (MQ=255)
                                                                           aCAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTc                                                               >  1:131072/1‑141 (MQ=255)
                                                                                  tACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTa                                                        <  1:558884/141‑1 (MQ=255)
                                                                                  tACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTa                                                        <  1:559271/141‑1 (MQ=255)
                                                                                    cTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATc                                                      <  2:602092/141‑1 (MQ=255)
                                                                                    cTCAAATTAGAGTCAATTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATc                                                      <  2:313145/141‑1 (MQ=255)
                                                                                     tCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACGGGTGGTATGAATACAACATAATCTTGCGCGAGTAGAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCTAAAGTGTCg                                                     >  1:586353/1‑141 (MQ=255)
                                                                                      cATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGt                                                    <  1:562100/141‑1 (MQ=255)
                                                                                      cATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGt                                                    <  2:57039/141‑1 (MQ=255)
                                                                                      cATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACACCATCAGGTGTACCAGTCGCAAGTATCGt                                                    >  1:398494/1‑141 (MQ=255)
                                                                                                    actactTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGt                                       <  1:110328/141‑1 (MQ=255)
                                                                                                      ttctTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACCGGTGGTCTGAATCCAAGATAATCTTGCGGTCGTATGGTATTGGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTAt                                     <  2:302491/139‑1 (MQ=255)
                                                                                                      tactTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAGTTGTATTATGCACCCCATCAGGTGTACCAGTCGCTAGTATCGTATAACCAGATGGTAt                                     >  1:420397/1‑141 (MQ=255)
                                                                                                       actTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTAtt                                    >  1:284576/1‑141 (MQ=255)
                                                                                                                taataaACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATAAAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTATAACAGTCGCAAGTATCGTTAACCCCAATGGTATTTGAACTGcc                           >  2:119501/1‑141 (MQ=255)
                                                                                                                       ctactGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTac                    >  2:549880/1‑141 (MQ=255)
                                                                                                                           tGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTaccacc                >  2:641491/1‑141 (MQ=255)
                                                                                                                             ccttAATTTGTTTAATTACAATTCGGAGTAATACCACATAATCGTTCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGTTGGTATTTGATCTGCCTCACTCCCACCAt              <  2:521646/138‑1 (MQ=255)
                                                                                                                              tctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAtt             >  1:107497/1‑141 (MQ=255)
                                                                                                                                tactttGTTTAACTACGGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCTGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCg           <  1:600444/138‑1 (MQ=255)
                                                                                                                                     tGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGtt      <  1:290230/141‑1 (MQ=255)
                                                                                                                                      ttttAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGttt     <  2:248150/140‑1 (MQ=255)
                                                                                                                                      gTTTAACTACAGGTGGTATGAATATAACATAATCTTACGGCTGTCTAGTAATGGTGTTATGCATCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGttt     >  2:274669/1‑141 (MQ=255)
                                                                                                                                       tttAACTACAGGTGGTATGAATACAGCATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATAGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTCGTTTg    >  2:435599/1‑141 (MQ=255)
                                                                                                                                       tttAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGTTTg    >  2:38807/1‑141 (MQ=255)
                                                                                                                                         tACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGGATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGTTTGAt  >  2:331684/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCGCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGTTTGAT  >  CP000730/1499238‑1499514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: