Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,812 G→A 54.1% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8120GA54.1% 3.7 / 156.2 118intergenic (‑895/‑273)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (17/37);  new base A (31/33);  total (48/70)
Fisher's exact test for biased strand distribution p-value = 9.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-02

Only 100 of 130 total aligned reads displayed.

TCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA  >  CP000730/1929672‑1929951
                                                                                                                                            |                                                                                                                                                 
tCGCCTACTTTCTTAAATTAAGAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTATAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtag                                                                                                                                                   >  1:344274/1‑141 (MQ=255)
   ccttccgtCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagtag                                                                                                                                                >  2:408338/8‑141 (MQ=255)
   ccTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAATGACTGTGCTTGTATATTTGCCGATGATATAAtagtgg                                                                                                                                                >  2:626350/1‑139 (MQ=255)
     gaCTTCCTTAAATTAATAAAACCTGTTCGCATAGAGTTTGTCGAATTAATTGTATACTCGTCAATCTATCAGCTAGAATCATATAACTGATCAGAAGCAAGGGACTGTGCTTGTATATTTGCAGCTGATATAATAGTagag                                                                                                                                              <  2:467327/140‑1 (MQ=255)
                     cAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAgg                                                                                                                              >  2:206125/1‑141 (MQ=255)
                        tatCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGt                                                                                                                           <  1:68097/141‑1 (MQ=255)
                         atCGATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTc                                                                                                                          <  1:510015/141‑1 (MQ=255)
                                 ccATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGa                                                                                                                  <  1:190073/140‑1 (MQ=255)
                                       aaTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCaga                                                                                                            <  2:372449/141‑1 (MQ=255)
                                        aTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAAGCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                                           <  1:115892/141‑1 (MQ=255)
                                         tttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagaga                                                                                                          >  2:81624/1‑141 (MQ=255)
                                          ttGTCCAATTAAGTGTAGACGATGCAATCTATCAGCGAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                         <  2:533477/141‑1 (MQ=255)
                                          ttGTCCAAATAAGTGGAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                         <  2:601120/141‑1 (MQ=255)
                                            gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAgagga                                                                                                       >  1:290132/1‑141 (MQ=255)
                                                 aTTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgtgt                                                                                                  <  2:585006/141‑1 (MQ=255)
                                                 aTTAAGGGGAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgtgt                                                                                                  <  1:70292/141‑1 (MQ=255)
                                                      gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAg                                                                                           <  2:553588/141‑1 (MQ=255)
                                                       tgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAgg                                                                                         <  2:500245/141‑1 (MQ=255)
                                                         tAGACGATTCAATCTGTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTAGAAGGTg                                                                                      >  1:333468/1‑141 (MQ=255)
                                                         tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTg                                                                                      >  2:280709/1‑141 (MQ=255)
                                                          aGACGATGCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGa                                                                                     <  2:101703/141‑1 (MQ=255)
                                                                 tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                                              <  2:100526/141‑1 (MQ=255)
                                                                 tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                                              <  1:510197/141‑1 (MQ=255)
                                                                 tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                                              <  1:558996/141‑1 (MQ=255)
                                                                   aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt‑‑tt                                                                          <  2:478985/141‑1 (MQ=255)
                                                                       tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGt                                                                      <  1:579224/141‑1 (MQ=255)
                                                                         tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTc                                                                    <  2:604374/141‑1 (MQ=255)
                                                                             cTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCAcat                                                                <  2:523844/141‑1 (MQ=255)
                                                                              tAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCAcatc                                                               <  2:307666/141‑1 (MQ=255)
                                                                                 aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTGTCTCTCAGGCGTCAATTTAGACGAACGAAGAAGCTTTGAAAGGTGATGCTTAT‑‑GTTGGGTCACATCATa                                                            >  1:345156/1‑141 (MQ=255)
                                                                                  aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCCTAg                                                           >  2:349720/1‑141 (MQ=255)
                                                                                  aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAg                                                           >  1:507327/1‑141 (MQ=255)
                                                                                    cATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTACGTTGGCATTATAGCa                                                         <  2:146991/141‑1 (MQ=255)
                                                                                     atatAACTTATCATAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCAc                                                        >  1:97926/1‑141 (MQ=255)
                                                                                                  gAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTgg                                           >  1:139232/1‑141 (MQ=255)
                                                                                                  atcggaagtatatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCGCAGGCGTCAAGT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑GTTCGTTCACATCATAGCACCAGTCACCAGTGGc                                          <  2:317311/127‑1 (MQ=255)
                                                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                          >  1:260077/15‑141 (MQ=255)
                                                                                                    cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATGGCACCAGTCATCAGTGGCtg                                        >  1:272690/13‑141 (MQ=255)
                                                                                                    cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATGCCACCAGTCATCGGTGGCtg                                        >  2:277398/13‑141 (MQ=255)
                                                                                                    cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGGCATAACTTTGCTAATCAT‑‑TCTCGTTCACATCATACCACCAGCCATAAGTGGCtg                                        >  1:382966/13‑141 (MQ=255)
                                                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                       >  2:277141/12‑141 (MQ=255)
                                                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                       <  1:408990/130‑1 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                    <  1:259322/133‑2 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                    <  1:195437/133‑2 (MQ=255)
                                                                                                         gtaggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCGGTCATCAGTGGCTGTGctg                                   <  1:354262/134‑3 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                   >  2:495662/8‑139 (MQ=255)
                                                                                                          tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTCTCTCTCATTCTTCAATT‑‑GACGAAGAGAGGAGGGGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                  <  1:288791/135‑4 (MQ=255)
                                                                                                          tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                  <  2:629877/135‑4 (MQ=255)
                                                                                                           gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTGT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                  >  1:553498/1‑141 (MQ=255)
                                                                                                            aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                 <  1:87817/141‑1 (MQ=255)
                                                                                                             cTGTGCTTGTATTTTTGCCGATGAAATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTg                                <  1:626350/141‑1 (MQ=255)
                                                                                                             cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTg                                <  1:408338/141‑1 (MQ=255)
                                                                                                              tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgcg                              <  1:205851/139‑8 (MQ=255)
                                                                                                               atgCTTGTATATTCACAGATGGCATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                             <  2:653165/140‑1 (MQ=255)
                                                                                                                tgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACACCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGt                             <  1:421486/141‑1 (MQ=255)
                                                                                                                  cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                          >  2:463506/1‑141 (MQ=255)
                                                                                                                  cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                          <  2:550349/141‑4 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                         <  1:538881/141‑5 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                         >  1:638863/1‑140 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                         <  2:356769/141‑5 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTCCTAATCAT‑‑TTTGGTTCACATCATAGCACCAGTCTTCAGTGGCTGTGCTGTTGCGtatta                         >  2:282518/1‑140 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTATAACAATAGAGTCTCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                         <  1:102810/141‑5 (MQ=255)
                                                                                                                    tGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGTCGCAGAGAGGAGGTGTATATGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                         <  1:424911/141‑1 (MQ=255)
                                                                                                                    tGTATATTCACAGTTGGTATAACAATAGAGTCGCCTATATCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGGTGCATTGCTTTGCTAATCAT‑‑TTTCGATCACATCATAGCACCAGTCATCAGGGGCTATGctgtggggtattat                        >  2:29800/1‑128 (MQ=255)
                                                                                                                    tGTATATTCACAGCTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                        <  1:278405/141‑6 (MQ=255)
                                                                                                                     gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                       >  1:242208/12‑138 (MQ=255)
                                                                                                                     gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGttttata                       >  1:575104/12‑138 (MQ=255)
                                                                                                                        tattcacagatggtATAATAATGGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                    <  2:274578/133‑7 (MQ=255)
                                                                                                                        tattatcagatggtATAATAATAGTGTTTGCTATCTCTCGGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                    <  1:120571/133‑7 (MQ=255)
                                                                                                                          ttcacagatggtATAATGATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGCGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTGCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                  >  1:469921/7‑141 (MQ=255)
                                                                                                                          ttcacagatggtATAATAATAGAGTCGCGTATCTCTCAGGCGTCAATT‑‑GACGAAAAGAGGAGGTGCATTACTTTGCTAAGCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                  >  1:429667/7‑141 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTCTCCTTCTTg                >  1:458604/5‑141 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                >  2:609170/5‑141 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTACACTTATTg                >  2:534203/5‑141 (MQ=255)
                                                                                                                             acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg               >  2:456364/4‑141 (MQ=255)
                                                                                                                              ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgg               <  1:448480/141‑1 (MQ=255)
                                                                                                                              ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgg               <  2:161790/141‑1 (MQ=255)
                                                                                                                              caggtggtataATAATAGAGTCGCCTATCTCTCATGCGTCAATT‑‑GACGGCGAGAGGGGGTGCATTACTTTGCTAATCAT‑‑TTTCGTCCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTTCGTATTATACTTATTGGc              <  2:285528/134‑1 (MQ=255)
                                                                                                                               agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt             <  1:437605/140‑1 (MQ=255)
                                                                                                                                tatggtATAATAATGGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCTTAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt            <  2:320702/140‑2 (MQ=255)
                                                                                                                                gattgaataacaATATAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTACTCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt            <  2:588350/135‑2 (MQ=255)
                                                                                                                                  tgttataATAATAGAGTCGTCTATCTCTCAGGCGTCAA‑‑TGGACGAATAGAGGAGGTCCATTACTTTGCTAATCCT‑‑TTTCGTTCGCATCATAGCACCAGTCAGCAGTGGCTGTGCTGTGGCGTATTATACTTATTGGCTTAg          <  2:270883/138‑1 (MQ=255)
                                                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag          <  1:612933/138‑4 (MQ=255)
                                                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag          <  2:197083/138‑4 (MQ=255)
                                                                                                                                  gagtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag          <  1:638521/138‑4 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTGTACTTATTGGCttggt         >  1:573848/3‑137 (MQ=255)
                                                                                                                                   gatATAATAGTAGAGCCGCCTATCTCTCAGGCGACAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTAGTTCACACCATAGAACCAGGCAACAGTGGCTGTGGCATTGCGTGTTTTTcatcttcactaat          >  2:625437/1‑129 (MQ=255)
                                                                                                                                    gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa        >  1:496047/2‑141 (MQ=255)
                                                                                                                                    gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGCCATCAGTGGCTGTGCTGTTGCGTATTATACTGATTGGCTTAGTa        >  1:74474/2‑141 (MQ=255)
                                                                                                                                    atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt         >  1:652215/1‑141 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa      <  1:445057/141‑3 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa      >  2:493284/1‑139 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa      >  1:354903/1‑139 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTT‑‑TGCTAATCATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc     >  2:388492/1‑141 (MQ=255)
                                                                                                                                       taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac     <  2:29742/141‑4 (MQ=255)
                                                                                                                                       aaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac     <  1:99481/140‑4 (MQ=255)
                                                                                                                                        aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaaag    >  2:647690/1‑137 (MQ=255)
                                                                                                                                        aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg    >  2:111394/1‑141 (MQ=255)
                                                                                                                                         ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTGAGTAGACg    >  2:122135/1‑141 (MQ=255)
                                                                                                                                         ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg    <  2:290132/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA  >  CP000730/1929672‑1929951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: