Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,865 A→C 55.5% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8650AC55.5% 75.3 / 183.2 158intergenic (‑948/‑220)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/38);  new base C (47/39);  total (79/79)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 158 total aligned reads displayed.

GTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCG  >  CP000730/1929726‑1930002
                                                                                                                                            |                                                                                                                                               
gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAg                                                                                                                                               <  2:553588/141‑1 (MQ=255)
 tgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAgg                                                                                                                                             <  2:500245/141‑1 (MQ=255)
   tAGACGATTCAATCTGTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTAGAAGGTg                                                                                                                                         >  1:333468/1‑141 (MQ=255)
   tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTg                                                                                                                                         >  2:280709/1‑141 (MQ=255)
           tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt                                                                                                                                 <  2:100526/141‑1 (MQ=255)
             aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAt‑‑tt                                                                                                                             <  2:478985/141‑1 (MQ=255)
                 tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGt                                                                                                                         <  1:579224/141‑1 (MQ=255)
                   tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTc                                                                                                                       <  2:604374/141‑1 (MQ=255)
                       cTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCAcat                                                                                                                   <  2:523844/141‑1 (MQ=255)
                           aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTGTCTCTCAGGCGTCAATTTAGACGAACGAAGAAGCTTTGAAAGGTGATGCTTAT‑‑GTTGGGTCACATCATa                                                                                                               >  1:345156/1‑141 (MQ=255)
                            aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCCTAg                                                                                                              >  2:349720/1‑141 (MQ=255)
                            aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAg                                                                                                              >  1:507327/1‑141 (MQ=255)
                              cATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTACGTTGGCATTATAGCa                                                                                                            <  2:146991/141‑1 (MQ=255)
                                        aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAC‑‑TTTCGCTCACAGCATAGCACCAGtcatca                                                                                                  <  2:472377/141‑1 (MQ=255)
                                            atcggaagtatatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCGCAGGCGTCAAGT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑GTTCGTTCACATCATAGCACCAGTCACCAGTGGc                                                                                             <  2:317311/127‑1 (MQ=255)
                                            atcggaagtagatatgCTTGTATATTCACAGATGGTATAATCAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                             <  1:513887/127‑1 (MQ=255)
                                            atcggaaggagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                             <  1:71181/127‑1 (MQ=255)
                                            aacggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                             <  2:316223/127‑1 (MQ=255)
                                             tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                            >  2:632488/14‑141 (MQ=255)
                                              cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATGGCACCAGTCATCAGTGGCtg                                                                                           >  1:272690/13‑141 (MQ=255)
                                              cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGGCATAACTTTGCTAATCAT‑‑TCTCGTTCACATCATACCACCAGCCATAAGTGGCtg                                                                                           >  1:382966/13‑141 (MQ=255)
                                               ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                          >  2:277141/12‑141 (MQ=255)
                                                  agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                       <  1:259322/133‑2 (MQ=255)
                                                   gtaggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCGGTCATCAGTGGCTGTGctg                                                                                      <  1:354262/134‑3 (MQ=255)
                                                   gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                      >  2:495662/8‑139 (MQ=255)
                                                    tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTCTCTCTCATTCTTCAATT‑‑GACGAAGAGAGGAGGGGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                     <  1:288791/135‑4 (MQ=255)
                                                    tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtgatgt                                                                                     <  1:478515/135‑5 (MQ=255)
                                                    tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                     <  2:629877/135‑4 (MQ=255)
                                                    tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                     <  1:26783/135‑4 (MQ=255)
                                                     gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTGT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                     >  1:553498/1‑141 (MQ=255)
                                                       cTGTGCTTGTATTTTTGCCGATGAAATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTg                                                                                   <  1:626350/141‑1 (MQ=255)
                                                       cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTg                                                                                   <  1:408338/141‑1 (MQ=255)
                                                       atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCCTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGCCTGTGCTAGTGc                                                                                  >  2:190326/4‑141 (MQ=255)
                                                        tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgcg                                                                                 <  1:205851/139‑8 (MQ=255)
                                                          tgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACACCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGt                                                                                <  1:421486/141‑1 (MQ=255)
                                                             ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                            <  1:538881/141‑5 (MQ=255)
                                                             ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                            <  2:356769/141‑5 (MQ=255)
                                                             ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                            >  1:638863/1‑140 (MQ=255)
                                                             ttGTATATTCACAGATGGTATAACAATAGAGTCTCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                            <  1:102810/141‑5 (MQ=255)
                                                              tGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGTCGCAGAGAGGAGGTGTATATGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                            <  1:424911/141‑1 (MQ=255)
                                                              tGTATATTCACAGTTGGTATAACAATAGAGTCGCCTATATCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGGTGCATTGCTTTGCTAATCAT‑‑TTTCGATCACATCATAGCACCAGTCATCAGGGGCTATGctgtggggtattat                                                                           >  2:29800/1‑128 (MQ=255)
                                                               gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                          >  1:242208/12‑138 (MQ=255)
                                                                  tattatcagatggtATAATAATAGTGTTTGCTATCTCTCGGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                                       <  1:120571/133‑7 (MQ=255)
                                                                  aatgcacagatggtAGAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTGGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                                       <  1:594747/133‑7 (MQ=255)
                                                                    ttcacagatggtATAATGATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGCGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTGCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                     >  1:469921/7‑141 (MQ=255)
                                                                     tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                    >  2:91718/6‑141 (MQ=255)
                                                                      cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTCTCCTTCTTg                                                                   >  1:458604/5‑141 (MQ=255)
                                                                      cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                   >  2:609170/5‑141 (MQ=255)
                                                                       acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                  >  2:456364/4‑141 (MQ=255)
                                                                        ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgg                                                                  <  1:448480/141‑1 (MQ=255)
                                                                        caggtggtataATAATAGAGTCGCCTATCTCTCATGCGTCAATT‑‑GACGGCGAGAGGGGGTGCATTACTTTGCTAATCAT‑‑TTTCGTCCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTTCGTATTATACTTATTGGc                                                                 <  2:285528/134‑1 (MQ=255)
                                                                         agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                <  1:437605/140‑1 (MQ=255)
                                                                          gattgaataacaATATAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTACTCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                               <  2:588350/135‑2 (MQ=255)
                                                                          gatggtATAATAATAGAGTCGCCTATCTCTCAGGCTTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                               <  2:536543/141‑2 (MQ=255)
                                                                          gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                               >  2:272694/1‑140 (MQ=255)
                                                                            tgttataATAATAGAGTCGTCTATCTCTCAGGCGTCAA‑‑TGGACGAATAGAGGAGGTCCATTACTTTGCTAATCCT‑‑TTTCGTTCGCATCATAGCACCAGTCAGCAGTGGCTGTGCTGTGGCGTATTATACTTATTGGCTTAg                                                             <  2:270883/138‑1 (MQ=255)
                                                                            tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                             <  1:612933/138‑4 (MQ=255)
                                                                            tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                             <  2:197083/138‑4 (MQ=255)
                                                                             ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTGTACTTATTGGCttggt                                                            >  1:573848/3‑137 (MQ=255)
                                                                             gatATAATAGTAGAGCCGCCTATCTCTCAGGCGACAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTAGTTCACACCATAGAACCAGGCAACAGTGGCTGTGGCATTGCGTGTTTTTcatcttcactaat                                                             >  2:625437/1‑129 (MQ=255)
                                                                              atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                            >  1:652215/1‑141 (MQ=255)
                                                                               tataATAATAGAGTCGCCTATCTCTCAGGCGTCGATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                          >  2:280738/1‑140 (MQ=255)
                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                         <  1:445057/141‑3 (MQ=255)
                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                         >  1:354903/1‑139 (MQ=255)
                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTT‑‑TGCTAATCATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                        >  2:388492/1‑141 (MQ=255)
                                                                                 taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                        <  2:29742/141‑4 (MQ=255)
                                                                                 aaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                        <  1:99481/140‑4 (MQ=255)
                                                                                  aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaaag                                                       >  2:647690/1‑137 (MQ=255)
                                                                                  aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                       >  2:111394/1‑141 (MQ=255)
                                                                                   ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTGAGTAGACg                                                       >  2:122135/1‑141 (MQ=255)
                                                                                   ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg                                                       <  2:290132/141‑1 (MQ=255)
                                                                                    taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                     >  1:566730/4‑141 (MQ=255)
                                                                                    taatagAGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                     <  2:376500/138‑1 (MQ=255)
                                                                                     aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                    <  1:613537/139‑1 (MQ=255)
                                                                                       tagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAt                                                   <  1:407826/141‑1 (MQ=255)
                                                                                        agctgctccTATCCCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAAGCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                 <  1:77642/134‑4 (MQ=255)
                                                                                        agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                 >  2:571661/1‑138 (MQ=255)
                                                                                        agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                 <  1:277141/141‑4 (MQ=255)
                                                                                           gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                               <  2:400338/141‑1 (MQ=255)
                                                                                           gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                              <  1:14081/141‑7 (MQ=255)
                                                                                            tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                             <  1:618995/141‑8 (MQ=255)
                                                                                            tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                             >  2:500234/1‑134 (MQ=255)
                                                                                            tCGCCTATCTCCCAGGCCTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                             <  1:8777/141‑8 (MQ=255)
                                                                                             cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                            >  1:261191/1‑133 (MQ=255)
                                                                                              gCCTATCTCTCAGGCGTCAATT‑‑GACGCGATGAGGGTGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaacaatt                                           <  2:300611/141‑10 (MQ=255)
                                                                                               ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                          >  1:81893/1‑131 (MQ=255)
                                                                                               ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                          >  2:641759/1‑131 (MQ=255)
                                                                                               ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCACCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                          <  1:97113/141‑11 (MQ=255)
                                                                                                cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                         >  1:144924/1‑130 (MQ=255)
                                                                                                 tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatttag                                        >  2:164795/1‑129 (MQ=255)
                                                                                                 tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                        >  1:508557/1‑129 (MQ=255)
                                                                                                 tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                        >  1:93114/1‑129 (MQ=255)
                                                                                                  aTCTCTCAGGCGTCAATT‑‑GCCGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatg                                       >  1:172730/1‑128 (MQ=255)
                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                       >  1:168804/1‑128 (MQ=255)
                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                       >  2:580444/1‑128 (MQ=255)
                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                       >  1:568979/1‑128 (MQ=255)
                                                                                                       tcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTAAAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTCGACGCAATACAAAATAGGTTACag                                   >  2:170845/1‑140 (MQ=255)
                                                                                                           gCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAg                               >  2:526166/1‑141 (MQ=255)
                                                                                                               cAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGc                           >  1:464158/1‑141 (MQ=255)
                                                                                                                aaTTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                          <  2:454536/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                               
GTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCG  >  CP000730/1929726‑1930002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: