Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,256,068 T→C 5.0% A144A (GCT→GCC deoD2 → purine‑nucleoside phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,256,0680TC 5.0% 213.8 / 3.0 80A144A (GCT→GCCdeoD2purine‑nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (39/35);  new base C (2/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

AGAAAATGTTAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCTGGTTTATATTTAA  >  CP000730/2255930‑2256207
                                                                                                                                          |                                                                                                                                           
aGAAAATGTCAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGa                                                                                                                                           <  1:413490/141‑1 (MQ=255)
  aaaaTGTTAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGCCTTCGACTTAGTAACTAAAGCTAAAAATGTCGCTGccc                                                                                                                                         >  2:551999/1‑141 (MQ=255)
    aaTGTTAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCTCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCaa                                                                                                                                       <  1:375941/141‑1 (MQ=255)
        ttAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCg                                                                                                                                   >  1:280054/1‑141 (MQ=255)
         tAACTTATACGATGTTATTATTGCACAAGCTTCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCgg                                                                                                                                  <  1:64468/141‑1 (MQ=255)
         tAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCgg                                                                                                                                  <  1:141503/141‑1 (MQ=255)
         tAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCgg                                                                                                                                  <  1:531976/141‑1 (MQ=255)
          aaCTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAATTAAAGCTAAAAATGTCGTTGTCCAAATCGGt                                                                                                                                 >  2:100795/1‑141 (MQ=255)
          aaCTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGt                                                                                                                                 <  1:535732/141‑1 (MQ=255)
          aaCTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGt                                                                                                                                 <  1:71484/141‑1 (MQ=255)
          aaCTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGt                                                                                                                                 >  1:177202/1‑141 (MQ=255)
             ttATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGct                                                                                                                              >  2:340010/1‑141 (MQ=255)
               ataCCATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGctac                                                                                                                            <  1:85083/141‑1 (MQ=255)
                   gATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCtactacc                                                                                                                        >  2:117832/1‑140 (MQ=255)
                   gATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGATACTaca                                                                                                                        >  2:554206/1‑141 (MQ=255)
                       ttattaTTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAACCATGTCGCGGACCAAATCGGTGCTACTACACACg                                                                                                                    <  2:113883/141‑1 (MQ=255)
                       ttattaTTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACg                                                                                                                    <  2:527063/141‑1 (MQ=255)
                        tattatGGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTTATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGt                                                                                                                   <  2:153296/141‑1 (MQ=255)
                         attactGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTGAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTa                                                                                                                  <  2:355202/141‑1 (MQ=255)
                          ttattGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAg                                                                                                                 <  2:88105/141‑1 (MQ=255)
                            attGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAGTACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGt                                                                                                               >  2:437397/1‑141 (MQ=255)
                            attGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACAAACTCGGTGCTACTACACACGTAAGt                                                                                                               >  2:102305/1‑141 (MQ=255)
                            attGCACAAGCTGCATCAACTAATTAAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAgga                                                                                                               >  2:157148/1‑140 (MQ=255)
                                 acaAGCTGCATCAACTAATTCAACATATTTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCTAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAActt                                                                                                          <  2:180161/141‑3 (MQ=255)
                                    aGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGGGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGtatt                                                                                                       >  1:15119/1‑141 (MQ=255)
                                      cTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGtattat                                                                                                     >  1:584325/1‑141 (MQ=255)
                                             aaCTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGa                                                                                              <  1:420088/141‑1 (MQ=255)
                                              aCTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGAt                                                                                             <  2:546358/141‑1 (MQ=255)
                                               cTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATa                                                                                            <  1:261568/141‑1 (MQ=255)
                                                   ttCAAATTATGTAGATCAATACAATATTCCTTGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACAtt                                                                                        <  1:252346/141‑1 (MQ=255)
                                                   ttCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACAtt                                                                                        <  2:177202/141‑1 (MQ=255)
                                                   ttCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACAtt                                                                                        >  1:533725/1‑141 (MQ=255)
                                                   atCAAAGTAAGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGCGGCTACTACACACGTAGGTAACGGATTATCTTCTGATACAtt                                                                                        <  1:100795/140‑1 (MQ=255)
                                                        aTTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACa                                                                                   <  1:551999/141‑1 (MQ=255)
                                                          taggTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTGTCTTTTGATACATTTTACAAt                                                                                 <  1:341351/138‑1 (MQ=255)
                                                           aTGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCCGACCAAATCGGTGCTACTACACACGTAGGTAACGTTTTATCTTCTGATACATTTTACAATg                                                                                >  1:34343/1‑141 (MQ=255)
                                                           aTGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCCGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATg                                                                                >  1:34351/1‑141 (MQ=255)
                                                             gTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGcc                                                                              <  1:340010/141‑1 (MQ=255)
                                                             gTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCACTGACTTCGAGTTATTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGAATTATCTTCTGATACATTTTACAATGcc                                                                              >  2:257321/1‑141 (MQ=255)
                                                                    aatacaataTTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAATGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGAGCCaa                                                                       >  1:158328/1‑141 (MQ=255)
                                                                    aatacaataTTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTGTTAATTTCTGATACAATTTACAATGCCGATCCaa                                                                       >  1:5878/1‑141 (MQ=255)
                                                                      tacaataTTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACa                                                                     <  2:520858/141‑1 (MQ=255)
                                                                         aataTTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTc                                                                  <  1:128378/141‑1 (MQ=255)
                                                                          atatTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCa                                                                 >  2:42598/1‑141 (MQ=255)
                                                                          atatTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCa                                                                 >  2:426056/1‑141 (MQ=255)
                                                                          atatTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCa                                                                 >  2:42482/1‑141 (MQ=255)
                                                                            atTCCAGGTCACTTCGCGCCTAGCGCTGACTTCGAGTTAGTAACGAAATCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAat                                                               <  1:499855/141‑1 (MQ=255)
                                                                             ttCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAatg                                                              <  2:280054/141‑1 (MQ=255)
                                                                                  ggTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCt                                                         >  1:377071/1‑141 (MQ=255)
                                                                                  ggTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATAACCTTCTGATACATTTTCCAATGCCGATCCAACATTCAATGATGCt                                                         >  2:242303/1‑141 (MQ=255)
                                                                                    tCATTTCGCGCCTATCGCTTACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACGCACGTAGGTAACGTATTATCTTCTTATACATTTTACAATGCCGATCCAACATTCAATGATGCTTg                                                       <  2:459344/141‑1 (MQ=255)
                                                                                    tCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTAAATGATGCTTg                                                       >  2:27452/1‑141 (MQ=255)
                                                                                      aTTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGCTCCAACATTCAATGATGCTTGGa                                                     >  1:152256/1‑141 (MQ=255)
                                                                                       tttCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGaa                                                    >  2:409650/1‑141 (MQ=255)
                                                                                       tttCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGCGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGCTGCTTGGaa                                                    >  2:76613/1‑141 (MQ=255)
                                                                                       tttCGAGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACAAAAACGGAGGTAATACAAACGTAGGTAGCGGATTATCTTCTTATACATTTTACAATGCAGATCCAACATTCAATGATGCTTGGaa                                                    >  2:237750/1‑141 (MQ=255)
                                                                                                        aCTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGGATTTTAg                                   >  1:582166/1‑141 (MQ=255)
                                                                                                          ttCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGt                                 >  1:21395/1‑141 (MQ=255)
                                                                                                           tCGAGTTAGTGACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTa                                >  1:483083/1‑141 (MQ=255)
                                                                                                            cGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTAt                               >  1:230105/1‑141 (MQ=255)
                                                                                                            cGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTAt                               <  1:36540/141‑1 (MQ=255)
                                                                                                            cGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACAAGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAACGATGCTTGGAAAAAAATGGGTATTTTAGGTAt                               >  1:444213/1‑141 (MQ=255)
                                                                                                              aGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACAACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCg                             >  2:216387/1‑141 (MQ=255)
                                                                                                               gTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATGCAACATTAAATGATGCTTGGAAAAAAATGGGTTTTTTAGGAATAGa                            >  2:172001/1‑141 (MQ=255)
                                                                                                                    ttaatAAAGCTAAATATGTCGTTGACCAAATCGGTGATACTTCACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGATTAGAAAAAAATGGGTATTTTAGGTATCGAAATgg                       <  2:320975/137‑1 (MQ=255)
                                                                                                                    tAACTAAAGCTAAAAATGTCGCCGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATgg                       <  2:34343/141‑1 (MQ=255)
                                                                                                                    tAACTAAAGCTAAAAATGTCGCCGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATgg                       <  2:34351/141‑1 (MQ=255)
                                                                                                                     aaCTAAAGCTAATAATGTCGTTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAATGGGTATTTTAGGTATCGAAATGGaa                     <  2:103560/141‑1 (MQ=255)
                                                                                                                      aCTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACTTTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGaa                     <  2:597511/141‑1 (MQ=255)
                                                                                                                            gCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGTAATCAGCt               >  1:582797/1‑141 (MQ=255)
                                                                                                                            gCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCt               <  1:352709/141‑1 (MQ=255)
                                                                                                                            gCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCt               >  1:64078/1‑141 (MQ=255)
                                                                                                                             cTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAACTGGGTATATTAGGTATCGAAAAGGAATCAGCTg              >  2:627482/1‑141 (MQ=255)
                                                                                                                                 aaaTGTCGCTGACCAAATCGGTGCTACTCAACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCCGGttt          >  1:421987/1‑141 (MQ=255)
                                                                                                                                    tggcGCTGACCAAATCGGTGGTAGTACAGAGGTAGGTAAGGGATTATCTTCTGATACATTTTACGAGGGCGTTCCAACTTTCAAAGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCTGGTTtata       <  2:458802/138‑1 (MQ=255)
                                                                                                                                    tGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGGATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCTGGTTtata       >  1:520588/1‑141 (MQ=255)
                                                                                                                                     gTCGCGGACCAAATCCGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACACTCTGCGACGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCTGGTTTatat      <  1:316710/141‑1 (MQ=255)
                                                                                                                                      tCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCTGGTTTATAtt     <  1:393362/141‑1 (MQ=255)
                                                                                                                                        gCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCTGGTTTATATTTa   <  1:426056/141‑1 (MQ=255)
                                                                                                                                         cTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCTGGTTTATAGTTaa  >  1:265953/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGAAAATGTTAACTTATACGATGTTATTATTGCACAAGCTGCATCAACTAATTCAAATTATGTAGATCAATACAATATTCCAGGTCATTTCGCGCCTATCGCTGACTTCGAGTTAGTAACTAAAGCTAAAAATGTCGCTGACCAAATCGGTGCTACTACACACGTAGGTAACGTATTATCTTCTGATACATTTTACAATGCCGATCCAACATTCAATGATGCTTGGAAAAAAATGGGTATTTTAGGTATCGAAATGGAATCAGCTGGTTTATATTTAA  >  CP000730/2255930‑2256207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: