Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,298,108	T→A	intergenic (+4/‑232)	pnp → / → rnjB	polyribonucleotide nucleotidyltransferase/beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,298,108	0	T	A	100.0%	131.9 / NA	42	intergenic (+4/‑232)	pnp/rnjB	polyribonucleotide nucleotidyltransferase/beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/28);  total (14/28)

ACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTAT  >  CP000730/1297975‑1298238                                                                                                                                      |                                                                                                                                   accaacttCAAAAAATAGAATGGAAAAAGTGGAAGATGTATGAAAAATCGGTGACAAAATTGAAGCTAAGATTACTGAATTTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTc                                                                                                                             <  1:226536/135‑1 (MQ=255) acaAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTc                                                                                                                             <  1:838696/141‑1 (MQ=255)  caAAATTCAAAAAATAGAAATGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCa                                                                                                                            <  1:603656/141‑1 (MQ=255)     atcacAAAAAAAAGAAAAGAAACAGTGGAACATGTATTAAAAATCGTCGACACAAGTGAAGTTAAGATTACTGAAATGGATAAACAAGCTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCatat                                                                                                                         <  1:68923/137‑1 (MQ=255)          aaaaaaTAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATgacga                                                                                                                    <  1:912562/141‑1 (MQ=255)           aaaaaTAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATgacgac                                                                                                                   >  1:943149/1‑141 (MQ=255)           aaaaaTAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATgacgac                                                                                                                   >  1:1048197/1‑141 (MQ=255)                    aTTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCg                                                                                                          >  1:115277/1‑141 (MQ=255)                    aTTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCg                                                                                                          >  1:623885/1‑141 (MQ=255)                          aaaGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCAtgt                                                                                                    <  1:1192619/141‑1 (MQ=255)                            aggggAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGa                                                                                                  <  1:1086220/138‑1 (MQ=255)                            aGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGa                                                                                                  <  1:894551/141‑1 (MQ=255)                              tGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGAtt                                                                                                >  1:24621/1‑141 (MQ=255)                                      gTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATg                                                                                        <  1:478271/141‑1 (MQ=255)                                                        acaATTGAAGTTAAGAGTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGa                                                                      >  1:174398/1‑141 (MQ=255)                                                          aaTTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGAcc                                                                    <  1:1028823/141‑1 (MQ=255)                                                             tGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGt                                                                 >  1:326314/1‑141 (MQ=255)                                                              gAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtt                                                                <  1:1121039/141‑1 (MQ=255)                                                              gAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtt                                                                <  1:847856/141‑1 (MQ=255)                                                                aGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGtttt                                                              >  1:895665/1‑141 (MQ=255)                                                                   tAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGGGCCTTAAAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGaa                                                           <  1:925526/141‑1 (MQ=255)                                                                   tAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGaa                                                           <  1:75129/141‑1 (MQ=255)                                                                     aGATTACTGAAATTGATCAACAAATTCGAGTAAATGCTTCACATAGCGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAAtt                                                         <  1:508818/141‑1 (MQ=255)                                                                      aaTTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAAttt                                                        <  1:1157640/140‑1 (MQ=255)                                                                        ttACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATttgt                                                      <  1:958530/141‑1 (MQ=255)                                                                             gAAATTGATAAACAAGGTCGAGCAAATGCTTCACATAGAGCACTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTAt                                                 <  1:29839/141‑1 (MQ=255)                                                                                 ttGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGACTTTGTTGTATTGaa                                             >  1:450130/1‑141 (MQ=255)                                                                                     tAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAAAAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACAtt                                         >  1:631131/1‑141 (MQ=255)                                                                                      aaaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGATTTTGTTGTATTGAACAttt                                        <  1:634508/141‑1 (MQ=255)                                                                                      aaaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACAttt                                        <  1:635118/141‑1 (MQ=255)                                                                                       aaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATATAGGCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACAtttt                                       >  1:930206/1‑141 (MQ=255)                                                                                            ggTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAAc                                  >  1:294933/1‑141 (MQ=255)                                                                                                 aGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCttt                             >  1:27684/1‑141 (MQ=255)                                                                                                 aGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCttt                             >  1:846173/1‑141 (MQ=255)                                                                                                            cacaTAGAGTATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTtgtg                  <  1:130456/141‑1 (MQ=255)                                                                                                             acaTAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTtgtgt                 <  1:325208/141‑1 (MQ=255)                                                                                                                    gCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATCATTTTGTGTGCAACTg          <  1:612517/141‑1 (MQ=255)                                                                                                                       ttAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTa       <  1:963307/141‑1 (MQ=255)                                                                                                                        tAGAAGTATAATATATAAAGTCATATGACGACCATGTTTTGTCATTTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTaa      <  1:513905/141‑1 (MQ=255)                                                                                                                           aagaaTAATATATAAAGTCATATGATGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTa   <  1:995685/141‑1 (MQ=255)                                                                                                                           aagaaTAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTa   <  1:176472/141‑1 (MQ=255)                                                                                                                            agaaTAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTAt  <  1:97511/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   ACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTAT  >  CP000730/1297975‑1298238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑