Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,499,376 C→A G883V (GGT→GTT)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,499,3760CA96.2% 163.6 / NA 52G883V (GGT→GTT) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (33/17);  minor base T (1/1);  total (34/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATT  >  CP000730/1499241‑1499503
                                                                                                                                       |                                                                                                                               
ccTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCGAAATAGTAGCAGCATTGCTCATAGTAGATTCAACTACGGTATTATAATAAACTAGTGCTCTAATTTGGTGATCTACa                                                                                                                            >  1:1140803/1‑141 (MQ=255)
     aTAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtgg                                                                                                                       <  1:913409/141‑1 (MQ=255)
     aTAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtgg                                                                                                                       >  1:659214/1‑141 (MQ=255)
     aTAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCCCTAATTTGTTTAACTACAggtgg                                                                                                                       >  1:488495/1‑141 (MQ=255)
       aGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTa                                                                                                                     <  1:667418/141‑1 (MQ=255)
       aGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAAAAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTa                                                                                                                     <  1:170913/141‑1 (MQ=255)
       aGTATTTGGCAGTTATTCCAACAGGAGTATTGATTGTTGGGGGAATGTCAGCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTa                                                                                                                     <  1:188091/141‑1 (MQ=255)
        gTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTCCAGGTGGTAt                                                                                                                    >  1:674569/1‑141 (MQ=255)
        gTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCACATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTAt                                                                                                                    <  1:904190/141‑1 (MQ=255)
          aTTTGGCATTTATTTCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACGGCTCTAATTTGTTTAACTACAGGTGGTATGa                                                                                                                  <  1:529453/141‑1 (MQ=255)
          aTTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGa                                                                                                                  <  1:529577/141‑1 (MQ=255)
                cATTTATTCCAACGGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACATCATTACTCATATTATATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATacaa                                                                                                            <  1:1136756/141‑1 (MQ=255)
                 aTTTATGCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTTGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATacaac                                                                                                           <  1:902739/141‑1 (MQ=255)
                  tttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATAATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATacaaca                                                                                                          >  1:1048823/1‑141 (MQ=255)
                   ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACAt                                                                                                         <  1:461393/141‑1 (MQ=255)
                      ttCCAACAGGATTATTGATTGTTGGTGGAATTTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACATGTGGTATGAATACAACATAAt                                                                                                      <  1:618773/141‑1 (MQ=255)
                       tCCCACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATc                                                                                                     <  1:381445/141‑1 (MQ=255)
                       tCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAACTTGTTTAACTACAGGTGGTATGAATACAACATAATc                                                                                                     <  1:1118246/141‑1 (MQ=255)
                               gATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTc                                                                                             >  1:610205/1‑141 (MQ=255)
                                 ttattGATTGTTGGTGGAATGTCATCTGGCAAAATCGTAACCATTTTTCGCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGt                                                                                           <  1:514929/141‑1 (MQ=255)
                                   attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAACTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGtat                                                                                         >  1:518586/1‑141 (MQ=255)
                                        ttgttgGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTATTTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTaa                                                                                    <  1:931634/141‑1 (MQ=255)
                                                  aaTGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTTTATTATg                                                                          >  1:649380/1‑141 (MQ=255)
                                                   aTGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGc                                                                         >  1:184389/1‑141 (MQ=255)
                                                                    aGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTAcc                                                        >  1:323766/1‑141 (MQ=255)
                                                                    aGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTAcc                                                        >  1:433729/1‑141 (MQ=255)
                                                                      tAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAg                                                      >  1:204359/1‑141 (MQ=255)
                                                                      tAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAg                                                      >  1:233783/1‑141 (MQ=255)
                                                                        aCAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTc                                                    <  1:684153/141‑1 (MQ=255)
                                                                          aGCATTACTCATATTAGATGCAATCACTTTATTATAATAAACTACTGCACTAATTGGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGc                                                  <  1:1050423/141‑1 (MQ=255)
                                                                               tACTCATATTAGATGGAACCACTTTATTATAATAAACTACTGATCTAATTTGTTTAACTACAGGCGGTATGATTTCAACATAATCTTGCGCTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCGGTCGCAAGTa                                             >  1:891820/1‑141 (MQ=255)
                                                                                       ttAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATaa                                     <  1:1062526/141‑1 (MQ=255)
                                                                                           aTGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAg                                 >  1:647036/1‑141 (MQ=255)
                                                                                            tGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGa                                >  1:150653/1‑141 (MQ=255)
                                                                                             gCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTAAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTCTAACCAGAt                               >  1:401437/1‑141 (MQ=255)
                                                                                                  tactTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTAt                          >  1:1033324/1‑141 (MQ=255)
                                                                                                   actTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTAtt                         >  1:688149/1‑141 (MQ=255)
                                                                                                        attatAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCGGAAGGTATTTGATc                    >  1:194401/1‑141 (MQ=255)
                                                                                                           ataataAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGc                 >  1:640052/1‑141 (MQ=255)
                                                                                                           ataataAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGACCAACGTCAGGTGTACCAGTCGCAAGTATGGTATAAACAGATGGTCTTTGATCTGc                 >  1:1050382/1‑141 (MQ=255)
                                                                                                                 aaCTACTGCTCTAATTCGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACt           >  1:712012/1‑141 (MQ=255)
                                                                                                                  actactGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTTTTCCAGTAGCAAGTATCGTATAACCAGAGGGTGTTTGATCTGCGTCACtc          >  1:503707/1‑140 (MQ=255)
                                                                                                                  actactGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTa          >  1:695789/1‑141 (MQ=255)
                                                                                                                      ctGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTaccac      >  1:679236/1‑141 (MQ=255)
                                                                                                                         ctctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCTCCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAt   >  1:322078/1‑141 (MQ=255)
                                                                                                                         ctctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCACGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAt   >  1:108194/1‑141 (MQ=255)
                                                                                                                         ctctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAt   >  1:434664/1‑141 (MQ=255)
                                                                                                                          tctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAtt  >  1:1139097/1‑141 (MQ=255)
                                                                                                                          tctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAtt  >  1:1138851/1‑141 (MQ=255)
                                                                                                                          tctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAtt  >  1:437934/1‑141 (MQ=255)
                                                                                                                          tctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCAtt  >  1:944661/1‑141 (MQ=255)
                                                                                                                          tctAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGCAGTCCCAGTCGCAAGTATCGTATAACCAGATGGGATTTGATCTGCCTCACTACCACCAtt  >  1:1170410/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                               
CCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATT  >  CP000730/1499241‑1499503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑