Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,851,260	C→A	A115S (GCG→TCG)	acsA1 ←	acetate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,851,260	0	C	A	100.0%	143.9 / NA	46	A115S (GCG→TCG)	acsA1	acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/18);  total (28/18)

GAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGCAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTC  >  CP000730/1851128‑1851389                                                                                                                                     |                                                                                                                                  gAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCAGAAATGCTTCAACTAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAAtata                                                                                                                           <  1:459078/141‑1 (MQ=255)        cAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAgg                                                                                                                    <  1:962919/141‑1 (MQ=255)           tGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACCATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGgtgt                                                                                                                 <  1:1055125/141‑1 (MQ=255)                      tAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAGAATACAGTTCAGGTGTACGCGACataa                                                                                                      >  1:736324/1‑141 (MQ=255)                        aTTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaat                                                                                                    <  1:970967/141‑1 (MQ=255)                          tAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaatat                                                                                                  <  1:621955/141‑1 (MQ=255)                           aaCACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGGGTACGCAGCataaatata                                                                                                 >  1:704835/1‑141 (MQ=255)                             cacTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaatataaa                                                                                               >  1:335866/1‑141 (MQ=255)                             cacTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaatataaa                                                                                               >  1:237911/1‑141 (MQ=255)                                  tAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATActc                                                                                          <  1:387263/141‑1 (MQ=255)                                    gCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTg                                                                                        >  1:93927/1‑141 (MQ=255)                                     cTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGt                                                                                       >  1:1015684/1‑141 (MQ=255)                                      ttCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTc                                                                                      >  1:1108155/1‑141 (MQ=255)                                      ttCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTc                                                                                      >  1:260149/1‑141 (MQ=255)                                      ttCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTGCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTc                                                                                      >  1:232603/1‑141 (MQ=255)                                            gTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCCCCttt                                                                                >  1:1064222/1‑141 (MQ=255)                                            gTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCttt                                                                                >  1:545966/1‑141 (MQ=255)                                               ctctAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTc                                                                             >  1:345171/1‑141 (MQ=255)                                                   aaTCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACt                                                                         <  1:99792/141‑1 (MQ=255)                                                   aaTCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACt                                                                         <  1:1032724/141‑1 (MQ=255)                                                   aaTCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACt                                                                         <  1:304512/141‑1 (MQ=255)                                                           cGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATg                                                                 >  1:357787/1‑141 (MQ=255)                                                             cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGtt                                                               >  1:384465/1‑141 (MQ=255)                                                             cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTGTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGtt                                                               >  1:795966/1‑141 (MQ=255)                                                             cAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCAAACAATTGCACCAATTTTTAAAAAACCAAACAACGAAAAATATAGTTCATGTGTACGCGACATAAATATAAGTACTATGTCACCTTGGTCAACTTCTGCagagt                                                               >  1:246319/1‑137 (MQ=255)                                                                 tGCCTTTTCCATAAATGCTTTAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAACTACCCTGTCACCTTTGTCAAGTTCTGCATGTTCAGa                                                           >  1:747522/1‑141 (MQ=255)                                                                  gCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGAc                                                          >  1:755180/1‑141 (MQ=255)                                                                   ccTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACa                                                         >  1:1011235/1‑141 (MQ=255)                                                                   ccTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACa                                                         >  1:283068/1‑141 (MQ=255)                                                                     ttttCCATAAATGCTTCAAATAACGGCCCAACAACTGCATCAATTTGTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACaaa                                                       <  1:1111982/141‑1 (MQ=255)                                                                       ttCCATAACTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAAc                                                     <  1:550649/141‑1 (MQ=255)                                                                             taacgctgccaaTAACGGCCCACCAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCgc                                               <  1:1160975/131‑1 (MQ=255)                                                                             aaaTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCgc                                               <  1:152473/141‑1 (MQ=255)                                                                             aaaTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCgc                                               >  1:1125281/1‑141 (MQ=255)                                                                                  cTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCtt                                          >  1:298787/1‑141 (MQ=255)                                                                                   ttCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCATGTGTACGCGACACAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCttt                                         >  1:22177/1‑141 (MQ=255)                                                                                     cAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAGCGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTttat                                       >  1:931659/1‑141 (MQ=255)                                                                                       aaTACCGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTttatta                                     <  1:666153/141‑1 (MQ=255)                                                                                            cGGCCCAACAATTGCACCAATTTTTAAAACACCAACCAACCAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATaa                                <  1:248006/141‑1 (MQ=255)                                                                                               cccAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCg                             >  1:1157720/1‑141 (MQ=255)                                                                                               cccAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCg                             >  1:946190/1‑141 (MQ=255)                                                                                                     aaTTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCat                       >  1:1062502/1‑141 (MQ=255)                                                                                                      aTTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCata                      <  1:502531/141‑1 (MQ=255)                                                                                                           aCCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCctt                 >  1:540140/1‑141 (MQ=255)                                                                                                                     aaaaCACCCAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACACAACAATCCCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATAc       <  1:631255/141‑1 (MQ=255)                                                                                                                          cccTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCCTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTc  <  1:819214/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  GAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGCAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTC  >  CP000730/1851128‑1851389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑