Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,543,088 G→A R283K (AGA→AAA)  hlgA → gamma‑hemolysin component A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,543,0880GA96.5% 179.4 / NA 58R283K (AGA→AAA) hlgAgamma‑hemolysin component A
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (33/23);  total (35/23)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAATCATCTTAA  >  CP000730/2542950‑2543224
                                                                                                                                          |                                                                                                                                        
aaCTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAAc                                                                                                                                        >  1:1007615/1‑141 (MQ=255)
aaCTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAAc                                                                                                                                        >  1:645859/1‑141 (MQ=255)
aaCTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAAc                                                                                                                                        >  1:536820/1‑141 (MQ=255)
aaCTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCAACATATGCTTACGTGACAAAAc                                                                                                                                        >  1:256253/1‑141 (MQ=255)
      ctcctTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGACATCGtt                                                                                                                                  >  1:606082/1‑141 (MQ=255)
      ctcctTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGtt                                                                                                                                  >  1:858830/1‑141 (MQ=255)
          tttAATTCAAAGTGGCTTTAATCCATCCTTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGc                                                                                                                              >  1:576967/1‑141 (MQ=255)
            tAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCg                                                                                                                            <  1:960969/141‑1 (MQ=255)
              aTTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCCTTTAGCCGtt                                                                                                                          <  1:864994/141‑1 (MQ=255)
               ttCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTg                                                                                                                         >  1:637505/1‑141 (MQ=255)
                     gTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGGAGAGACATGGATGCTACATAAGCTTACGTGACAAAACATCGTTTAGCCGTTGCTAGaa                                                                                                                   >  1:866697/1‑141 (MQ=255)
                      tGGCTTTAATCCATCATTTCTTACAACACTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATAGTTTAGCCGTTGATAGaaa                                                                                                                  <  1:635794/141‑1 (MQ=255)
                         cTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACa                                                                                                               <  1:108163/141‑1 (MQ=255)
                         cTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACa                                                                                                               <  1:28127/141‑1 (MQ=255)
                          tttAATCCAGCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACat                                                                                                              >  1:875677/1‑141 (MQ=255)
                              aTCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACatgatg                                                                                                          >  1:292858/1‑141 (MQ=255)
                                  atcatTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCttt                                                                                                      <  1:865707/141‑1 (MQ=255)
                                  atcatTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTAAGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCttt                                                                                                      <  1:416207/141‑1 (MQ=255)
                                   tcatTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGGTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGGTGCgttt                                                                                                     >  1:66271/1‑141 (MQ=255)
                                     atTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAAAATGATGCTTTTaa                                                                                                   >  1:840919/1‑141 (MQ=255)
                                      tttATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGTCGTTGATAGAAAACATGATGCTTTTaaa                                                                                                  >  1:128728/1‑141 (MQ=255)
                                       ttattaCAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTaaaa                                                                                                 <  1:200636/141‑1 (MQ=255)
                                       ttactaCAACATTGTCACACAAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTaaaa                                                                                                 <  1:12645/141‑1 (MQ=255)
                                          ttaCAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACACGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAAcc                                                                                              >  1:403276/1‑141 (MQ=255)
                                           taCAACATTGTCACACGAAAGAGGTAAAGGGGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCg                                                                                             <  1:3517/141‑1 (MQ=255)
                                            acaacaTTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCATTGATAGAAAACATGATGCTTTTAAAAACCGa                                                                                            >  1:505208/1‑141 (MQ=255)
                                               acaTTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATAATGCTTTTAAAAACCGAAAc                                                                                         >  1:907469/1‑141 (MQ=255)
                                                  ttGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGtt                                                                                      <  1:840872/141‑1 (MQ=255)
                                                      cacaCGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAg                                                                                  >  1:12701/1‑141 (MQ=255)
                                                       acacGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGt                                                                                 >  1:114010/1‑141 (MQ=255)
                                                        cacGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATCGAGAACAGTTTGCTTTTAAAAACCGAAACGTTACAGtt                                                                                <  1:757522/141‑1 (MQ=255)
                                                              agagGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAatat                                                                          <  1:654938/141‑1 (MQ=255)
                                                                          gATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTgg                                                              <  1:1173881/141‑1 (MQ=255)
                                                                              aaaGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGaaaa                                                          >  1:421663/1‑141 (MQ=255)
                                                                              aaaGCGAGTTTGAAATCACTGACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGaaaa                                                          >  1:727130/1‑141 (MQ=255)
                                                                                aGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATAAAGTGAACTGGAAAaca                                                        >  1:24212/1‑141 (MQ=255)
                                                                                aGCGAGTTTGAAATAACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATAATGCATTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAaca                                                        >  1:625390/1‑141 (MQ=255)
                                                                                                   aCGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACGGTTAAATATGAAGTGAACTGGAAAATGCGTGAAGTAAAAATTaaaa                                     >  1:24972/1‑141 (MQ=255)
                                                                                                   aCGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTaaaa                                     >  1:83338/1‑141 (MQ=255)
                                                                                                    cGGCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAg                                    <  1:349267/141‑1 (MQ=255)
                                                                                                     ggCAGAAACATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGc                                   <  1:655215/141‑1 (MQ=255)
                                                                                                          aaaCATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATcac                              <  1:126600/141‑1 (MQ=255)
                                                                                                            aCATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCacac                            >  1:714067/1‑141 (MQ=255)
                                                                                                            aCATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCacac                            <  1:687469/141‑1 (MQ=255)
                                                                                                            aCATGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCacac                            >  1:659953/1‑141 (MQ=255)
                                                                                                             catgcatgCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGCAAACCTGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACAcc                           >  1:815920/1‑141 (MQ=255)
                                                                                                              aTGGATGCTACATATGCTTACGTGACAAGACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGATACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCt                          >  1:771466/1‑141 (MQ=255)
                                                                                                               tGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAACAACATGAAGTAAAAACTAAAAGCATCACACCTa                         >  1:365244/1‑141 (MQ=255)
                                                                                                               tGGATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTa                         >  1:351716/1‑141 (MQ=255)
                                                                                                                ggATGCTACATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTaa                        >  1:736755/1‑141 (MQ=255)
                                                                                                                       aCATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATCGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACa                 >  1:1124505/1‑141 (MQ=255)
                                                                                                                       aCATATGCTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTTAAAATTAAAAGCATCACACCCAAGTAAACa                 >  1:606228/1‑141 (MQ=255)
                                                                                                                              cTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAat          >  1:48873/1‑141 (MQ=255)
                                                                                                                              cTTACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAat          <  1:243514/141‑1 (MQ=255)
                                                                                                                              cTTACCTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAat          <  1:173368/141‑1 (MQ=255)
                                                                                                                                tACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAatca        <  1:646338/141‑1 (MQ=255)
                                                                                                                                tACGTGACAAAACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAatca        <  1:902835/141‑1 (MQ=255)
                                                                                                                                      aCAAAACATCTTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAATCATCTTaa  <  1:1138885/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
AACTACCTCCTTTAATTCAAAGTGGCTTTAATCCATCATTTATTACAACATTGTCACACGAAAGAGGTAAAGGTGATAAAAGCGAGTTTGAAATCACTTACGGCAGAAACATGGATGCTACATATGCTTACGTGACAAGACATCGTTTAGCCGTTGATAGAAAACATGATGCTTTTAAAAACCGAAACGTTACAGTTAAATATGAAGTGAACTGGAAAACACATGAAGTAAAAATTAAAAGCATCACACCTAAGTAAACAGTTCAATCATCTTAA  >  CP000730/2542950‑2543224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: