Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,842 Δ1 bp 100% intergenic (‑925/‑243) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8420T.57.3% 57.4 / 304.7 150intergenic (‑925/‑243)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/34);  new base . (47/39);  total (76/74)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 152 total aligned reads displayed.

TCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACC  >  CP000730/1929703‑1929980
                                                                                                                                           |                                                                                                                                               
tctcAGAGAATTTGCCCAATTAAGTGTAGACGATTCAATCTAGCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAttt                                                                                                                                                 <  2:176771/140‑1 (MQ=255)
     tAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTAACAAAAGAAAGTGACTGAGCTTGTAACTTTGCCGATGATATAATAATAGAGTCGCCTATATCCCAGGCGACAATTTAGaag                                                                                                                                            >  2:72305/1‑138 (MQ=255)
            tGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCTTATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                                                     <  1:87393/140‑1 (MQ=255)
               ctaagaaaGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAACAGCAAGTGACTGTGCTTGTATAGTTGCCGATGATATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATTTAGATGCAGAgagggg                                                                                                                                  >  2:173036/7‑138 (MQ=255)
                       gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAATAGAATCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGAGTA‑Taa                                                                                                                         >  1:324369/1‑140 (MQ=255)
                        tgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑g                                                                                                                       >  1:177297/1‑140 (MQ=255)
                              cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑g                                                                                                              <  1:107495/140‑1 (MQ=255)
                                 ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCtt                                                                                                           >  2:116665/1‑140 (MQ=255)
                                 ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCtt                                                                                                           <  1:455596/140‑1 (MQ=255)
                                 ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCtt                                                                                                           >  2:180334/1‑140 (MQ=255)
                                    aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCTTATCTCTCCGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTAtt                                                                                                        <  1:28187/140‑1 (MQ=255)
                                    aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCGTA‑TAC‑G‑G‑TGAT‑GCTTAtt                                                                                                        >  2:420496/1‑140 (MQ=255)
                                     aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTAttt                                                                                                       >  2:127954/1‑140 (MQ=255)
                                        tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCg                                                                                                    <  1:398272/140‑1 (MQ=255)
                                        tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCg                                                                                                    <  1:327701/140‑1 (MQ=255)
                                              cTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTcaca                                                                                              <  1:195487/140‑1 (MQ=255)
                                                  aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCAcatcat                                                                                          <  1:523940/140‑1 (MQ=255)
                                                      atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCa                                                                                      <  1:599815/140‑1 (MQ=255)
                                                      atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCATATTTTCGTTCACATCATAGcc                                                                                      >  2:601680/1‑139 (MQ=255)
                                                          aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAg                                                                                  <  2:232125/140‑1 (MQ=255)
                                                                 cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                                                                           >  1:642826/1‑140 (MQ=255)
                                                                  aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTA‑TTTCGTTCACATCATAGCACCAGTCATCAGTg                                                                         <  2:561362/140‑1 (MQ=255)
                                                                   atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAATCAACAGTgg                                                                        >  2:194350/15‑140 (MQ=255)
                                                                   atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCAACAGTgg                                                                        <  2:592135/126‑1 (MQ=255)
                                                                   atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTgg                                                                        >  2:553749/15‑140 (MQ=255)
                                                                    tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                       >  1:80227/14‑140 (MQ=255)
                                                                      ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                     <  2:564867/129‑1 (MQ=255)
                                                                       gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGTGTTGACTAACTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                    <  1:314332/130‑1 (MQ=255)
                                                                        aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                   <  1:506592/131‑1 (MQ=255)
                                                                         agtacatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                  <  2:484059/132‑1 (MQ=255)
                                                                         aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                   >  2:506101/1‑140 (MQ=255)
                                                                         aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                   <  1:519469/140‑1 (MQ=255)
                                                                         aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGACGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                   >  2:240119/1‑140 (MQ=255)
                                                                          gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCCCCCCAAACCACCAGTCATCAGTGGCTGTGct                                                                 <  1:490424/133‑2 (MQ=255)
                                                                          gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                 <  2:654528/133‑2 (MQ=255)
                                                                          gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                 <  1:312212/133‑2 (MQ=255)
                                                                           tagatatgCTTGTATATTTACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                <  1:169529/134‑3 (MQ=255)
                                                                           tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGcc                                                                 <  1:249326/140‑1 (MQ=255)
                                                                            agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GTCGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                               <  1:230962/135‑4 (MQ=255)
                                                                            agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                               <  1:118944/135‑4 (MQ=255)
                                                                            agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                               <  2:25474/135‑4 (MQ=255)
                                                                             gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtgatgtt                                                              >  2:484475/5‑135 (MQ=255)
                                                                             gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                              >  2:103259/5‑136 (MQ=255)
                                                                             gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                              <  1:246185/136‑5 (MQ=255)
                                                                             aCTGCGCTTCTATATTTGCCGATGATATAATAGTAGAGTCGCCTATTTCTCAGGCGTTAATTTAGACGTAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                               <  1:315532/140‑1 (MQ=255)
                                                                              cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                              >  1:407211/1‑140 (MQ=255)
                                                                              atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                             >  1:602345/4‑135 (MQ=255)
                                                                              atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                             >  1:236127/4‑135 (MQ=255)
                                                                               tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑TTA‑ACGAAGAAAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtttc                                                            >  1:554713/3‑134 (MQ=255)
                                                                               tatgCTTGTATATTCACAGAGGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                            <  2:267487/138‑7 (MQ=255)
                                                                                gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTTTCGCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                            <  1:123323/140‑1 (MQ=255)
                                                                                    ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                       <  2:340199/140‑4 (MQ=255)
                                                                                    ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                       <  1:500520/140‑4 (MQ=255)
                                                                                      gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                     <  1:652921/140‑3 (MQ=255)
                                                                                         tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                  >  2:424596/9‑135 (MQ=255)
                                                                                           ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                >  1:444181/7‑140 (MQ=255)
                                                                                            tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                               >  2:223806/6‑140 (MQ=255)
                                                                                             cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGATGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                              >  1:297137/5‑140 (MQ=255)
                                                                                             cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                              >  1:262654/5‑140 (MQ=255)
                                                                                              acagatagtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                             <  2:152124/132‑1 (MQ=255)
                                                                                               ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTg                                             >  2:156940/1‑140 (MQ=255)
                                                                                               cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAAAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGGTAACAACATAGGACCAGTCATCAGTGGCTGTGCTGTTTCGTATTATACTTATTgg                                            >  1:337951/3‑140 (MQ=255)
                                                                                                agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCAATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                           <  2:383188/139‑1 (MQ=255)
                                                                                                 gatgatATAATAGTAAAGTCGCCTATCTCTCAGGCGTCAATTTATACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                           <  1:110705/140‑1 (MQ=255)
                                                                                                    ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                       <  1:426444/138‑4 (MQ=255)
                                                                                                    ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTACTATACTTATTGGCTTAg                                       <  2:335683/138‑1 (MQ=255)
                                                                                                     ttataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                      <  2:615958/139‑1 (MQ=255)
                                                                                                     atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                       <  2:658037/140‑1 (MQ=255)
                                                                                                       cgatctatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTGACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                    >  2:6591/8‑139 (MQ=255)
                                                                                                       ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCCGTGGCTGTGCTGTTGCGTATTATACTTCTTGGcggagaat                                    >  2:135965/1‑133 (MQ=255)
                                                                                                       ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                    >  1:374131/1‑139 (MQ=255)
                                                                                                         aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                  <  1:540592/140‑4 (MQ=255)
                                                                                                         aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                  >  1:667840/1‑140 (MQ=255)
                                                                                                          ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCT‑TTA‑G‑T‑TCTTAAAGCAATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                 <  1:154182/140‑1 (MQ=255)
                                                                                                              tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                             >  2:353430/1‑139 (MQ=255)
                                                                                                                 agacGCCTATCTCTCAGGCGACAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                          <  1:179096/137‑5 (MQ=255)
                                                                                                                 agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCATTTTTTTCTTATTGGCTAAGTAGACGCAATAc                           >  2:371126/1‑140 (MQ=255)
                                                                                                                  gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                         >  1:631400/1‑135 (MQ=255)
                                                                                                                  gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                         >  1:438656/1‑135 (MQ=255)
                                                                                                                  gTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGATCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATCCTTATTGGCTTAGTAAACGCaaaaaat                         >  2:188322/1‑135 (MQ=255)
                                                                                                                  gTCGCCTATATCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                         >  2:251746/1‑135 (MQ=255)
                                                                                                                   tCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                         >  1:284975/1‑140 (MQ=255)
                                                                                                                   tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑TCTCAATTGCCGCCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                        <  1:484475/140‑7 (MQ=255)
                                                                                                                   tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                        >  2:540239/1‑134 (MQ=255)
                                                                                                                     gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTTTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACTCAATACaaaa                       >  1:467819/1‑140 (MQ=255)
                                                                                                                     gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                      >  1:186500/1‑132 (MQ=255)
                                                                                                                      cctctctttacggCGTAAATTGAGACCCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGTT‑GGTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGCAATACAAAAt                      <  2:179563/129‑1 (MQ=255)
                                                                                                                      ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATATTGGCTAAGTAGACGCAATACAAAAt                      >  1:264990/1‑140 (MQ=255)
                                                                                                                      ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                     >  2:324772/1‑131 (MQ=255)
                                                                                                                       cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                    >  2:100951/1‑130 (MQ=255)
                                                                                                                        tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTCC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGATGTGCTGTTGCGTATTATACTTATTAGCTTAGTAAACACaacaagtaattta                   >  1:649359/1‑129 (MQ=255)
                                                                                                                        tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                   >  1:418785/1‑129 (MQ=255)
                                                                                                                        tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                   >  1:307337/1‑129 (MQ=255)
                                                                                                                         aTCTCTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                  <  2:214678/140‑13 (MQ=255)
                                                                                                                         aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattact                  >  2:624595/1‑128 (MQ=255)
                                                                                                                                aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata            >  2:53056/1‑140 (MQ=255)
                                                                                                                                     tccaTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGcc       <  2:654316/137‑1 (MQ=255)
                                                                                                                                       aaTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGc     >  2:298503/1‑140 (MQ=255)
                                                                                                                                        aTTTAGGCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa    <  2:117695/140‑1 (MQ=255)
                                                                                                                                          ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTTACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAcc  >  2:355254/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                               
TCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACC  >  CP000730/1929703‑1929980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑