Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 18.3% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT18.3% 219.2 / 38.7 106intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (41/43);  new base T (7/12);  total (49/57)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-02

Only 100 of 106 total aligned reads displayed.

ATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATT  >  CP000730/76043‑76318
                                                                                                                                         |                                                                                                                                           
aTTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCta                                                                                                                                           >  1:599161/1‑140 (MQ=255)
     atatAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                      >  1:100003/1‑140 (MQ=255)
       ataACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGtata                                                                                                                                    >  2:253646/1‑140 (MQ=255)
          aCTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAta                                                                                                                                 >  2:263616/1‑139 (MQ=255)
            tAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                               <  1:107539/140‑1 (MQ=255)
                     tgcACTTTTATCACGTAGAACAGCTTACCAAAGCTTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATAATAGCAAAATATTGGTTCTGTTGCAAAGTTGAATCTAAAGTATAATTTTAACAAAAAg                                                                                                                      <  1:403753/140‑1 (MQ=37)
                         ccTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGt                                                                                                                  <  1:625803/140‑1 (MQ=255)
                         ccTGTAACACTTAGAACAGCTAAACAAAGAGTTAAAAAACCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTCCTGTTGCAAAGTTGAATCTATAGTATAAAATAAAAAAAAAGGAGt                                                                                                                  <  2:515915/140‑1 (MQ=255)
                           tGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtct                                                                                                                >  2:321524/1‑140 (MQ=255)
                            gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTCACAAAAAGGAGtctt                                                                                                               >  1:82917/1‑140 (MQ=255)
                            gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                               <  1:244791/140‑1 (MQ=255)
                            gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAAGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                               >  1:288582/1‑140 (MQ=255)
                            gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGAGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                               >  2:433213/1‑140 (MQ=255)
                             tATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                              <  1:524716/140‑1 (MQ=255)
                                      gAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCCAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                    <  1:457608/140‑1 (MQ=255)
                                       aaCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACt                                                                                                   <  1:287599/140‑1 (MQ=255)
                                        aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTa                                                                                                  >  1:220637/1‑140 (MQ=255)
                                         cAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAAAACAAAGGCAATTAATTTAATACTTATAGAAAAATATAGGTACTGTTGACAAGTAGAATCACAAGTCTACTTTTAAACAAAAAGAGACTTCTGtgggaccgct                                                                                                 >  2:627406/1‑131 (MQ=255)
                                            cTAACCAAAGTGTTAGTACTTCAATTAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTCACAAAAAGGCGCCTTCTGTATGAACTCTTTc                                                                                              >  2:144223/1‑140 (MQ=25)
                                              aaCCAAGGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAg                                                                                            >  2:369187/1‑140 (MQ=255)
                                               aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGa                                                                                           >  1:529453/1‑140 (MQ=255)
                                               aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAACAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAAATTAACAAAAAGGAGTCTACTGGATGAACTATTTCAGa                                                                                           >  1:619973/1‑140 (MQ=37)
                                                   aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAtata                                                                                       >  1:473249/1‑140 (MQ=255)
                                                       gtTAGTAATCCAAAGAATACTAACACAAAGGCAATTAATTCAATAATCATAGCAAAATATAGGTTCTGTTGCAAAGTTAAATCTATAGTATAATTTTAACAAAAACGAGTCTTCTGTATGAACTATTTCAGATATAAACa                                                                                   <  2:231490/140‑1 (MQ=21)
                                                              aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAc                                                                            >  2:443488/1‑140 (MQ=255)
                                                                ccaaagAACACCAACACAAAGACAACTAAATAAACAAATATAGCAAAACATACGTTCAGTTGCAAAGCTGAATCTATAGTAAAAAAAAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACaa                                                                          <  2:278716/135‑1 (MQ=9)
                                                                    ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGTACTATTTCAGATATAAACAATTTAACAAGGAt                                                                      <  1:477663/140‑1 (MQ=255)
                                                                    ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                      >  1:261732/1‑140 (MQ=255)
                                                                    ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAt                                                                      >  1:414058/1‑140 (MQ=255)
                                                                           tAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAAGTTCTATTACATAGATGTATCTATAGTATAATTTAGAAGCACTTTATGCATCGTGAATAACTATGTCAGATATAAACAATTTAACAAGGATGTTATCa                                                               >  2:638909/1‑140 (MQ=16)
                                                                             acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACt                                                             <  1:409394/140‑1 (MQ=255)
                                                                              cacaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTTCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                            <  1:147998/140‑1 (MQ=255)
                                                                                 aaaGGAAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTCAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                         <  2:365173/140‑1 (MQ=255)
                                                                                  aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAGTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGc                                                        <  1:569821/140‑1 (MQ=255)
                                                                                   aGGCAATTAATTTAATATTTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTAGAGTATAAATTTAACAAAAAGTATTCTTCTGTATTAACTATTTCAGTTATAAACAATTTAACAAGGATGTTATCCCTGTAGcc                                                       <  2:432648/140‑1 (MQ=18)
                                                                                   aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                       <  2:30639/140‑1 (MQ=255)
                                                                                       aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATATTTTTAACAAAAAGGAGTCTTCTGTAGGAACTATTTCCGATATAAACAATGTAACAAGGATGTTATCACTGTAGCCGTTg                                                   <  1:321524/140‑1 (MQ=255)
                                                                                        attaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                  <  2:599161/140‑1 (MQ=255)
                                                                                          taatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTCTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                <  2:510042/140‑1 (MQ=255)
                                                                                          taatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                <  1:562120/140‑1 (MQ=255)
                                                                                          taatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGAAGCCGTTGGct                                                >  1:205483/1‑140 (MQ=255)
                                                                                              ttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctacta                                            >  1:368468/1‑140 (MQ=255)
                                                                                              ttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctacta                                            >  1:368652/1‑140 (MQ=255)
                                                                                              ttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctacta                                            >  2:615768/1‑140 (MQ=255)
                                                                                              ttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGACTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAAGTTATCACTGTAGCCGTTGGctacta                                            >  1:492187/1‑140 (MQ=255)
                                                                                                 ataatTATAGCAAAATATAGTTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCt                                         <  1:33441/140‑1 (MQ=255)
                                                                                                 ataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCt                                         <  1:253646/140‑1 (MQ=255)
                                                                                                     ttATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGa                                     <  1:658797/140‑1 (MQ=255)
                                                                                                        tttcAAATTATAGGTTCGGTTGCAAAGTTCAATCCATAATAGAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATGTAAGATATAAACAAATTAACAATTGTGTTATCACTGTAGCCGTTGGCTACTATCTAAGagat                                  <  2:163737/137‑1 (MQ=11)
                                                                                                         aGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATg                                 <  2:100003/140‑1 (MQ=255)
                                                                                                         aGCAAAATATAGGTTCTGTGGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCGGTTGGCTACTATCTAAGATATg                                 <  2:486527/140‑1 (MQ=255)
                                                                                                           cAAAATATGAGTTCTGTTTCCAAGTTTAATCTATAGAAGAATTTTACCAAAAAGGAGGCTTCTGTAGGAACTATTTCAGATATAAACATTTTAACAAGGATGTTATCACTGTAGCCCTTGGCTACTATCTAAGATATGCa                               <  2:189982/140‑1 (MQ=11)
                                                                                                            gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                              >  1:504351/8‑140 (MQ=11)
                                                                                                            aaaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGGCGTTGGCTACTATCTAAGATATGCAt                              >  2:580203/1‑140 (MQ=255)
                                                                                                            aaaaTATAAGTTTTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                              <  2:255157/140‑1 (MQ=255)
                                                                                                              ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                            >  1:572509/6‑140 (MQ=0)
                                                                                                              ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                            <  2:379143/135‑1 (MQ=255)
                                                                                                              ttaggaGGTTCTGTTACAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                            >  1:495713/6‑140 (MQ=1)
                                                                                                              ttagaaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGGTATAAAAAATTGAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                            <  1:247719/135‑1 (MQ=11)
                                                                                                              aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGCTATGCATTg                            >  2:487413/6‑140 (MQ=1)
                                                                                                              aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                            <  1:562195/135‑1 (MQ=2)
                                                                                                              aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                            <  2:253548/135‑1 (MQ=255)
                                                                                                              aaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTGAACAAGGATGTTATCACTGTAGCCGATAGGTACTATCTAAGATATGCATTg                            <  2:473249/140‑1 (MQ=255)
                                                                                                               aatgaGGTTCTGTTGTAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCGTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                           <  2:55178/136‑1 (MQ=11)
                                                                                                               aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTGAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCAATTTATCTGATGGCTACTATCTAAGATATGCATTGa                           <  2:112955/136‑1 (MQ=11)
                                                                                                                tataCGTTCTGTTGCAAAGTTGAATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                          <  2:145586/140‑1 (MQ=11)
                                                                                                                atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                          <  1:489412/137‑1 (MQ=2)
                                                                                                                aggaGGTTCTGTGTCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                          <  1:163193/137‑1 (MQ=11)
                                                                                                                 tgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                         <  2:476766/138‑1 (MQ=9)
                                                                                                                 ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGt                         >  2:586013/1‑140 (MQ=39)
                                                                                                                 ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGAAATATTATGTATGAAATATTTAAGATATAAAAAATTTAAAAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGATATGCATTGAGt                         >  1:188018/1‑140 (MQ=11)
                                                                                                                  taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGCACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGCTATGCATTGAGtt                        >  1:467033/1‑140 (MQ=255)
                                                                                                                  taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                        <  1:542183/140‑1 (MQ=37)
                                                                                                                  gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                        <  1:580860/139‑1 (MQ=2)
                                                                                                                  gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                        <  2:119271/139‑3 (MQ=9)
                                                                                                                   acgTTCTGTTGCAAAGTTGAATGTATAATATAATTTTAACAAAAAGGATTCTTTTTTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTa                       <  1:514460/138‑1 (MQ=11)
                                                                                                                   aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                       >  2:64898/1‑140 (MQ=2)
                                                                                                                   aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAttaggcta                       >  2:512151/1‑134 (MQ=1)
                                                                                                                    ggatCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAGACAGGCGGCTCCTGTATGAACTATTTCAGATCTAAACAATTTAACAAGGACGTTATCACCATAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                      >  2:246447/4‑140 (MQ=11)
                                                                                                                    ggTTCTGTTGCAAAGTTGAGTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                      >  1:352780/1‑140 (MQ=21)
                                                                                                                     gTTCTGTTGCAAAGTTGAATATATAGTATAATTTTAACAAAAAGGAGTCTACAGTATGAACTATTTCAGATATAAACAATTGAACAAGAATGTTATCACTGTAGCAGTTGGCTACTAACTAAGATATGCATTGAGTAATc                     >  2:71773/1‑140 (MQ=11)
                                                                                                                       tCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                   <  1:580345/140‑1 (MQ=35)
                                                                                                                       tCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                   <  2:459302/140‑1 (MQ=35)
                                                                                                                       tCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCCTCTGTATTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                   <  1:492048/140‑1 (MQ=255)
                                                                                                                        cTGTTGCAAAGTTGAATCTATAGTAAAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTTAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTg                  <  1:634008/140‑1 (MQ=25)
                                                                                                                         tGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                 <  2:384727/140‑1 (MQ=37)
                                                                                                                          gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGAATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGat                >  1:351155/1‑140 (MQ=25)
                                                                                                                          gTTGCAAAGTTGAATCCATAGTATAAACCTACGAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGat                >  2:276998/1‑140 (MQ=11)
                                                                                                                           ttGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATATAAGATATGCATTGAGCTATCGTGata               >  2:644102/1‑140 (MQ=255)
                                                                                                                             gCAAAGTTGAATCTATAGTATAATTTTAACAAAACGGAGTCTCCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCCCTGTAGCCGTTGACTACTATCTAAGATATGCATGGGGTTAACGTGatatt             >  2:516570/1‑139 (MQ=11)
                                                                                                                             gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatata             >  2:290624/1‑140 (MQ=34)
                                                                                                                             gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatata             >  2:493886/1‑140 (MQ=35)
                                                                                                                              cAAAGTTGAATATATAGTAAAAAAAAAAAAAAGAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat            <  2:373292/140‑1 (MQ=11)
                                                                                                                               aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc           >  1:607451/1‑140 (MQ=37)
                                                                                                                                 aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg         <  1:586013/140‑1 (MQ=35)
                                                                                                                                    tGAAACAATACTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaaa      <  2:162442/140‑1 (MQ=16)
                                                                                                                                      aaTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAata    <  1:638909/140‑1 (MQ=35)
                                                                                                                                        tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg         <  1:84463/133‑1 (MQ=34)
                                                                                                                                        tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg         >  2:84463/1‑133 (MQ=33)
                                                                                                                                        tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt  >  1:574061/1‑140 (MQ=33)
                                                                                                                                         |                                                                                                                                           
ATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATT  >  CP000730/76043‑76318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: