Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,322 G→A 56.0% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3220GA56.0% 101.9 / inf 439intergenic (+380/‑754)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (95/97);  new base A (134/109);  total (232/207)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

TAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAAT  >  CP000730/76183‑76458
                                                                                                                                           |                                                                                                                                        
  ttataTTTTTATCTAAAAGGGGTCTTCTGTATGAATTATTTCAGTTATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                                                                        <  2:22101/139‑1 (MQ=11)
     taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                                                                     >  1:100391/1‑140 (MQ=12)
       aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                                                                                   <  2:535605/140‑1 (MQ=18)
        ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCTTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                                                                                                                  <  2:17213/140‑1 (MQ=17)
        ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGCTATATCTGAAAGATTAAGGGAACgtgt                                                                                                                                  >  1:617501/1‑139 (MQ=12)
        ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                                                                                  <  2:506083/140‑1 (MQ=16)
        ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATATAATTCAATAAATGCATTGAGGTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                                                                                                                  >  1:153188/1‑140 (MQ=11)
         tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggt                                                                                                                                 >  1:75162/1‑140 (MQ=12)
          ttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                                                                                <  1:556290/140‑1 (MQ=16)
            aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTa                                                                                                                              >  2:321978/1‑140 (MQ=17)
             aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                                             >  1:508945/1‑140 (MQ=17)
               aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTAGCACTGTAGCTTTTGCCTAATAAATGACCCATGCAGGGAGGCATCGTTATGTATCTGAAAGATTAAGGTAACGTGGTGTAAAc                                                                                                                           >  2:638729/1‑140 (MQ=2)
                aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGGGGTGTAAACg                                                                                                                          >  2:560619/1‑140 (MQ=255)
                 aacggAGTCTACTGTATGAACTAATTCAGATTTAAACAACTTAACAAGGATTTTATCACTGTAGCCGTTGGCTACTATCTAAGATAGGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                                         <  2:129347/137‑1 (MQ=11)
                 aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAGTTAACGAGGATGTTATAACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                                         >  1:320295/1‑140 (MQ=11)
                  aaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATTTGCAATATTAAGAGAACGTGGTGTAAACGtt                                                                                                                        >  1:584369/1‑140 (MQ=255)
                   aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTGTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAACTATCATGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                                                       >  1:386608/1‑140 (MQ=11)
                   aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTTAGTTATCGTGATATATTTGAAATAGTAAGGGCACGTGGTGTAAACGTtc                                                                                                                       >  2:651455/1‑140 (MQ=11)
                     gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAAGGTtcat                                                                                                                     >  2:341045/1‑140 (MQ=12)
                      aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGCTGTTATCACTGTAGCCGTTGACTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACTTGGTGTAAACGTtcatc                                                                                                                    >  1:235360/1‑140 (MQ=11)
                      aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                                                                                    >  1:532718/1‑140 (MQ=16)
                       gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                                                                                   >  1:271769/1‑140 (MQ=18)
                        tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGCAGGTGGTGTAAACGTTcatcat                                                                                                                  >  1:163180/1‑140 (MQ=255)
                         cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTAATCAtt                                                                                                                 >  2:396495/1‑140 (MQ=17)
                         cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATAAAGCGCACTTGGTGTAAACGTTCAGCAtt                                                                                                                 >  2:329413/1‑140 (MQ=11)
                           tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCa                                                                                                               >  2:35847/1‑140 (MQ=18)
                            ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCaa                                                                                                              >  2:62445/1‑140 (MQ=16)
                            ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAAAGTTAATCATTCac                                                                                                              >  2:471576/1‑139 (MQ=11)
                             tGTTTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGGTATCGTGATATATCTGAAGTATTAAGGGAACGTGGGGTAAACGTTCATCATTCAAc                                                                                                             >  2:280412/1‑140 (MQ=11)
                              gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGATCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                                            <  2:488983/140‑1 (MQ=255)
                              gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTAGACTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                                            >  1:171415/1‑140 (MQ=255)
                                 tGAACTTTTTCAGATATAAACAATTTAACGAGGATGTTATCACTGTAGGCGTTCGGTACGATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAGTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATc                                                                                                         <  1:315792/140‑1 (MQ=11)
                                 tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                         <  1:612135/140‑1 (MQ=18)
                                 tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAAAGTAGTGTAAACGTTCATCATTCAACGATc                                                                                                         >  1:119877/1‑140 (MQ=11)
                                 tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATc                                                                                                         >  2:401996/1‑140 (MQ=12)
                                 tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGACAACGTGGTGTAAACGTTCATCATTCAACGATc                                                                                                         >  2:42707/1‑140 (MQ=255)
                                  gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGtag                                                                                                        <  2:368468/140‑3 (MQ=18)
                                   aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGTATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTa                                                                                                       >  1:94822/1‑140 (MQ=255)
                                    aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                                                      >  2:596815/1‑140 (MQ=33)
                                       ctttCAGATATAAACAATGTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                   <  2:375284/139‑1 (MQ=12)
                                        tttCAGATATAAACAATTTAACAAGGATATTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                  >  1:429384/1‑140 (MQ=255)
                                        ttgcAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                  <  2:414058/137‑1 (MQ=17)
                                          tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                                                                                >  1:521586/1‑140 (MQ=17)
                                           cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                               <  1:662111/140‑1 (MQ=12)
                                           cAGATACAAACAATTTAACAAGGATGTTATCACTGTAGCTTTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTTAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                               <  1:2787/140‑1 (MQ=255)
                                            aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                         >  2:157803/1‑129 (MQ=18)
                                            aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                              <  2:152398/140‑1 (MQ=255)
                                                  aaaaaaTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCGTTGGGTTCAAGa                                                                                        <  2:440318/140‑1 (MQ=12)
                                                  aaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                                                        <  1:443488/140‑1 (MQ=33)
                                                     cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTAATATATCTCGGTGACGAAGAGAAAGTGGTGTAAACGTTCATCATTCAACAATCTACCGTTGGGTTGAAGAata                                                                                     >  1:654218/1‑140 (MQ=11)
                                                      aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGCTATGCATTGAGCTATCGTGATATATCTGAAATATTATGAGAACTTGGGGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                    <  1:56896/140‑1 (MQ=11)
                                                      aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGTAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAatat                                                                                    <  2:82917/140‑1 (MQ=17)
                                                      aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                    <  2:665956/140‑1 (MQ=255)
                                                       aTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                                                                                   <  2:258034/140‑1 (MQ=17)
                                                         ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTCAGATATGCATTGAGTTATCGTGTTATATCTTAAATATTAAGGTAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                                                 <  1:486920/140‑1 (MQ=255)
                                                         ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                 <  1:247296/140‑1 (MQ=12)
                                                         ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                 <  2:50029/140‑1 (MQ=255)
                                                         ttAACAAGGATGTTAGCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                 <  2:609179/140‑1 (MQ=255)
                                                            aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                                              >  2:518189/1‑140 (MQ=255)
                                                            aCAAGGATGTTATCACAGTAGTTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCTCCa                                                                              >  1:341086/1‑140 (MQ=255)
                                                            aCAACGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAAGGAACGTGGTGTAAACGTTCATCATTCAGCGGGCTACCGATGGGTTCAAGAATATGCCCCa                                                                              >  1:453189/1‑140 (MQ=11)
                                                             cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACTTTCATCAATCAACGGTCTACCGTTGGGTTCAAGAATATGTCCCaa                                                                             >  2:14045/1‑140 (MQ=12)
                                                                    gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTCCCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                      >  1:203162/1‑140 (MQ=255)
                                                                    gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                      <  1:62445/140‑1 (MQ=255)
                                                                    gTTATCACTGGAGCCGTTGGCTACTATCTAAGATATGCATCGAGCTATCGTGTTATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                      <  2:69470/140‑1 (MQ=11)
                                                                     ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                     <  2:1260/140‑1 (MQ=255)
                                                                      tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                    >  1:376359/1‑140 (MQ=255)
                                                                      tATCACTGTAGCCGCTGGCTACTATCTAAGATATGCATTGAGCTCTCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                                                    >  1:364152/1‑140 (MQ=11)
                                                                       aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                                                   >  1:602526/1‑140 (MQ=255)
                                                                        tCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGGGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCCAGAATATGCCCCAATTTTATATCaa                                                                  >  2:553482/1‑140 (MQ=255)
                                                                         cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                 >  2:249722/1‑140 (MQ=255)
                                                                         cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                 >  2:632962/1‑140 (MQ=255)
                                                                          aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCACCAATTTTATATCAAAt                                                                >  2:570565/1‑140 (MQ=255)
                                                                           cTGTAGGCGTTGGTGAGTATCTAAGAGATGCATGGCGGTATCGTGATATATCTGCAGTATTAAGGGAACGTGGTGTAAACGTTGATCATCCAACGATCTAGCGTTGGGTTCAATAATATGCCCCAATGTTTTATTAAAtt                                                               <  2:118376/140‑1 (MQ=2)
                                                                              tAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACAGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                            <  1:187851/140‑1 (MQ=255)
                                                                               aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                           <  1:413670/140‑1 (MQ=255)
                                                                               aGCCGTTGGCTACGATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                           <  1:476647/140‑1 (MQ=255)
                                                                               aGCCGTTGGCTAATATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                           <  2:287331/140‑1 (MQ=255)
                                                                                gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                          >  1:194368/4‑140 (MQ=25)
                                                                                gcagTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAATAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                          >  2:389513/4‑140 (MQ=11)
                                                                                gCCGTTGGCTACTATCTAAGTTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTTGTGTAAACGTTCATTATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                          <  1:25682/140‑1 (MQ=11)
                                                                                gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAAAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTAGGGTTCAAGAATAAGCCCCAATTTTATATCAAATCTGgaa                                                          >  1:146696/1‑140 (MQ=11)
                                                                                 ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                         <  1:380947/140‑1 (MQ=255)
                                                                                 ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                         >  2:585549/1‑140 (MQ=255)
                                                                                        ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                  >  2:617349/1‑140 (MQ=35)
                                                                                          actaTCTAAGGTATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                <  1:515757/140‑1 (MQ=21)
                                                                                          actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                <  2:302362/140‑1 (MQ=35)
                                                                                          aataTGTAAGATATGCATTTGGTTTTCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                <  1:127782/138‑1 (MQ=11)
                                                                                           ctaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                               >  1:460423/1‑140 (MQ=37)
                                                                                            taTCTAAGATATGCATTGAGTTCTCGTGATATATTTGAAATATTAAGGGCACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                              <  2:454320/140‑1 (MQ=255)
                                                                                            taTCTAAGATAAGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                              <  2:91742/140‑1 (MQ=25)
                                                                                             aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                             >  1:392563/1‑140 (MQ=35)
                                                                                               cTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                                           <  1:108091/140‑1 (MQ=255)
                                                                                                tAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaacg                                          >  1:64226/1‑138 (MQ=21)
                                                                                                    atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagctt                                      >  1:295094/1‑135 (MQ=11)
                                                                                                        gCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTAAACGGTCTACCGTTGGGTTCAAGCCTATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTAc                                  >  1:136537/1‑140 (MQ=255)
                                                                                                                                   atatTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATc       <  2:352780/140‑1 (MQ=40)
                                                                                                                                    tatTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGCTGAGACGTACATCa      >  2:209126/1‑140 (MQ=255)
                                                                                                                                       tAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGATAATTACAAATGGCGTATTGACGAGACGCACACCcaaa   >  2:531713/1‑140 (MQ=14)
                                                                                                                                        aaGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGACATCAAAAATAAGCCACAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAAt  >  1:22013/1‑140 (MQ=14)
                                                                                                                                           |                                                                                                                                        
TAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAAT  >  CP000730/76183‑76458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: