Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,353 G→A 51.5% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3530GA51.5% 2.7 / inf 335intergenic (+411/‑723)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (77/85);  new base A (89/82);  total (167/168)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 337 total aligned reads displayed.

TATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCC  >  CP000730/76214‑76489
                                                                                                                                           |                                                                                                                                        
tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                                                                                          <  1:549904/140‑1 (MQ=32)
  taaaCTATTTCAGATATAAACAATTTAAAATGTATGTTATCAATGTAGTCGTTGGTTACTATCTAAGATATGCTTGGAGTTATTGTGATATATCTGAAATAGTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                                                        <  1:41655/138‑1 (MQ=11)
  tGAACTTTTTCAGATATAAACAATTTAACGAGGATGTTATCACTGTAGGCGTTCGGTACGATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAGTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATc                                                                                                                                        <  1:315792/140‑1 (MQ=11)
  tGAACTAGTTTAGATATACACGATTTAACAAGTATGTTATCACTGTAGCCGTTGGCTACTATCTAAGTTATGCTTTGAGCTATCGTGATTTATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATc                                                                                                                                        <  1:168729/140‑1 (MQ=11)
   gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGtag                                                                                                                                       <  2:343681/140‑3 (MQ=18)
    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTa                                                                                                                                      >  1:242705/1‑140 (MQ=18)
    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGTATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTa                                                                                                                                      >  1:94822/1‑140 (MQ=255)
      cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                                                                                                    >  2:36623/1‑140 (MQ=255)
         tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                                                 >  2:660807/1‑140 (MQ=255)
         tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                                                 >  1:383203/1‑140 (MQ=12)
         ttgcAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                 <  2:414058/137‑1 (MQ=17)
          ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGTCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                                                >  1:187911/1‑140 (MQ=11)
          ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTAGTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                                                >  1:441653/1‑140 (MQ=255)
          ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTTAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                                                                                                                <  2:20842/140‑1 (MQ=255)
           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGATGTCACCGTTCATCCTTCAACGATCTACCGttag                                                                                                                               >  2:128857/1‑138 (MQ=11)
            cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                              <  1:662111/140‑1 (MQ=12)
            aacataaAAACAAGTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGTATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                              <  1:61228/137‑1 (MQ=11)
             aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTc                                                                                                                                        >  2:157803/1‑129 (MQ=18)
             aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                                                                             <  2:152398/140‑1 (MQ=255)
               atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTc                                                                                                                           >  1:141598/1‑140 (MQ=32)
               atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTc                                                                                                                           >  1:196235/1‑140 (MQ=12)
                tataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                                                                                          <  1:219058/140‑1 (MQ=17)
                tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                                                                                                          <  1:263616/140‑1 (MQ=32)
                  taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGTCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                                                                        <  1:411854/140‑1 (MQ=17)
                  taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                                                                        <  2:562546/140‑1 (MQ=255)
                  taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTAGTGTAAACGTTCTTCATTCAACGATCTACTGTTGGGTTCAAg                                                                                                                        >  1:114902/1‑140 (MQ=11)
                   aaaaaaTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCGTTGGGTTCAAGa                                                                                                                       <  2:440318/140‑1 (MQ=12)
                   aaaaaaTTTAACAAAGATGTTATCACTGGATCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTTAACGGTCTACCGTTGGGTTCTAGa                                                                                                                       <  2:120483/140‑1 (MQ=11)
                   aaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                       >  2:615418/1‑140 (MQ=255)
                     aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATCTCTGCAATATTTACGGTGCGGGTTGTAGTAGATCATCATTCAACGGTCTACCGTTGGGTTCAAGAat                                                                                                                     <  2:470005/140‑1 (MQ=11)
                       aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAatat                                                                                                                   <  2:665956/140‑1 (MQ=255)
                        aTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                                                                                                                  <  2:258034/140‑1 (MQ=17)
                        aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                                                  <  1:100462/140‑1 (MQ=255)
                          ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                                                <  2:50029/140‑1 (MQ=255)
                          ttAACAAGGATCTTATCACTGTAGCCGTTGGCCACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAGGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCTTTGGGTTCAAGAATATGcc                                                                                                                <  2:372566/140‑1 (MQ=11)
                            aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCGTTGGGTTCAAGAATCTGcccc                                                                                                              >  1:248301/1‑140 (MQ=255)
                            aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                                                                              <  2:169427/140‑1 (MQ=12)
                            aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                                                                                              >  2:620042/1‑140 (MQ=255)
                            aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGGTGGGTTCAAGAATATGcccc                                                                                                              >  1:638185/1‑140 (MQ=11)
                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTGCAAGAATATGCCCCa                                                                                                             >  1:534126/1‑140 (MQ=17)
                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                                                                             >  2:518189/1‑140 (MQ=255)
                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGGTCATCATTCAACGATCTAACGTTGAGTTCAAGAATATACCCCa                                                                                                             >  2:633035/1‑140 (MQ=11)
                             aCAAGGATGTTATCACAGTAGTTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCTCCa                                                                                                             >  1:341086/1‑140 (MQ=255)
                             aCAAGGATGTTATAACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATTTCTGAAATATTAAGGGAACGTGGTGTAATCGTTTATCATAAAACGTTCTACAGTTGGGTTCAAGAATATGCCCCa                                                                                                             >  1:451320/1‑140 (MQ=11)
                              cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACTTTCATCAATCAACGGTCTACCGTTGGGTTCAAGAATATGTCCCaa                                                                                                            >  2:14045/1‑140 (MQ=12)
                                aGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGAAGAGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                                                                                          >  1:276465/1‑140 (MQ=11)
                                    tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                         <  1:286991/137‑1 (MQ=255)
                                    tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                                                                         >  2:286991/1‑137 (MQ=255)
                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTCCCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                     >  1:203162/1‑140 (MQ=255)
                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                     <  1:62445/140‑1 (MQ=255)
                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                     <  1:80629/140‑1 (MQ=255)
                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATGTttta                                                                                                     >  1:573529/1‑140 (MQ=11)
                                     gTTATCACTGTAGCCGTTGGCTACTATCAAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                     >  1:430704/1‑140 (MQ=255)
                                     gTTATCACTGGAGCCGTTGGCTACTATCTAAGATATGCATCGAGCTATCGTGTTATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                     <  2:69470/140‑1 (MQ=11)
                                          cACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                                                >  1:357953/1‑140 (MQ=37)
                                          cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                                                                                >  2:249722/1‑140 (MQ=255)
                                           actggtgCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                                                                               <  1:565397/134‑1 (MQ=11)
                                              gTAGCCGTTGGCTAATATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                                                                            <  2:62795/140‑1 (MQ=25)
                                               tagccaacggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                           >  2:80962/9‑140 (MQ=11)
                                                aGCCGTTGGCTACGATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                          <  2:540204/140‑1 (MQ=255)
                                                 gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                         >  1:512643/4‑140 (MQ=21)
                                                 gcagTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAATAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                         >  2:389513/4‑140 (MQ=11)
                                                 gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                         >  2:593509/1‑140 (MQ=255)
                                                  ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                        >  1:380372/1‑140 (MQ=255)
                                                  ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                        >  1:130214/1‑140 (MQ=255)
                                                    aacagCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                                                      >  1:149411/5‑140 (MQ=37)
                                                     ttGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                                                                                     >  1:501724/1‑140 (MQ=255)
                                                         ctactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                                                 <  1:521273/140‑1 (MQ=255)
                                                           actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                               <  2:302362/140‑1 (MQ=35)
                                                           actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGAAAGAAAAAGCAt                                                                               >  1:232678/1‑140 (MQ=255)
                                                           accagcTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                               <  1:648239/135‑1 (MQ=21)
                                                            ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                              <  2:640485/140‑1 (MQ=255)
                                                             taTCTAAGATATGCATTGAGTTCTCGTGATATATTTGAAATATTAAGGGCACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                                                                             <  2:454320/140‑1 (MQ=255)
                                                              atatAAGATATGCATTGAATTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                            <  2:92031/137‑1 (MQ=21)
                                                              aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGACCGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                            >  1:656181/1‑140 (MQ=25)
                                                              aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                            <  2:402337/140‑1 (MQ=35)
                                                              aTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa                                                                            <  1:124983/140‑1 (MQ=255)
                                                               tCTAATATATGCATTGAGTTATCGTGATATATCTTAAATATTAAGTGAACGTGTTGTAAACGTTTATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                           <  1:160943/140‑1 (MQ=11)
                                                               tCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                           <  1:46015/140‑1 (MQ=255)
                                                               tCTAAGATATGCATTGAGCTATCGGTATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                           <  2:325055/140‑1 (MQ=11)
                                                                cTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                                                                          <  1:108091/140‑1 (MQ=255)
                                                                 tAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                                                         <  1:375577/140‑2 (MQ=21)
                                                                   aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaaga                                                                       >  2:42872/1‑137 (MQ=255)
                                                                     atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAGGCATaaaaaagctt                                                                     >  1:174156/1‑135 (MQ=11)
                                                                      tgtacATTGAGCTATGGTGATATTTCTAAAATAGTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCAAATTTTATATAAAATTTGGAAGAAAAAGCATaaaaacacttg                                                                    >  2:489932/5‑134 (MQ=11)
                                                                       atGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                                                   <  2:587668/140‑8 (MQ=1)
                                                                       atGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat                                                                   <  2:559067/140‑8 (MQ=1)
                                                                        tGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTa                                                                  >  1:318142/1‑140 (MQ=40)
                                                                         gCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTACAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGATAATTAc                                                                 >  1:466030/1‑140 (MQ=255)
                                                                           aTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTTGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACaa                                                               <  2:397033/140‑1 (MQ=255)
                                                                                 ttATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCg                                                         <  1:596815/140‑1 (MQ=40)
                                                                                    tCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTAt                                                      >  1:390007/1‑140 (MQ=39)
                                                                                                    atatTAAGGGAGCGTGGTGTAAACTGTCATTATTCAACGGTTTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATc                                      <  2:220637/140‑1 (MQ=14)
                                                                                                        tAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCaaca                                  >  1:610379/1‑138 (MQ=39)
                                                                                                         aaGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTAGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAAt                                 >  2:356210/1‑140 (MQ=255)
                                                                                                                  tggtgTAAACGATCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAggcac                        >  2:289518/1‑137 (MQ=255)
                                                                                                                  gggtgAAAACGTTCATCATTCATCGGGCTACGGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGaaa                        <  2:529453/139‑1 (MQ=255)
                                                                                                                            gTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTAt              >  2:598629/1‑140 (MQ=40)
                                                                                                                                   atTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGACGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATc       >  1:47563/1‑140 (MQ=255)
                                                                                                                                        aCGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGcc  <  2:574061/140‑1 (MQ=40)
                                                                                                                                           |                                                                                                                                        
TATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCC  >  CP000730/76214‑76489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: