Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,651 C→T 11.0% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6510CT11.0% 184.8 / 12.3 74D170D (GAC→GATUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (23/42);  new base T (5/3);  total (29/45)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01

AGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  CP000730/110514‑110790
                                                                                                                                         |                                                                                                                                           
agatagtcagTTAAACTTGTTGAGAATAAAACCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                           <  2:555544/131‑1 (MQ=17)
aGATATCCGGTTAAAATTGTCGATAATAAATTCATTCCAACAAAAGTAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                           <  1:131136/140‑1 (MQ=255)
  atatCCGGTTAAAATGGTAGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGTCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                         <  2:260620/140‑1 (MQ=255)
    atCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGat                                                                                                                                       <  1:128201/140‑1 (MQ=255)
    atCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGat                                                                                                                                       <  1:456370/140‑1 (MQ=255)
      ccGGTTAAAATGGTCGATAATAAAATAATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTGTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATATAAAAAATTATAAAAAGGgaaaaat                                                                                                                                     >  2:50685/1‑135 (MQ=17)
        ggTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAAAAATGGCTATTATAAAGACGGAGATAttt                                                                                                                                   <  1:660476/140‑1 (MQ=14)
        ggTTAAAATAGTCTATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                   <  2:633028/140‑1 (MQ=255)
         gTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTc                                                                                                                                  <  1:627446/140‑1 (MQ=255)
           tAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCat                                                                                                                                <  2:607879/140‑1 (MQ=255)
             aaaaGGTCAATAATAAAATCATTCCAACAGAATAAATTACAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCatat                                                                                                                              <  2:371993/140‑1 (MQ=17)
                tGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTAAAAAATTATAAAGACAGAGATATTTAATATAAt                                                                                                                           >  1:336940/1‑140 (MQ=255)
                     gATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATTATTCATTTAATCCaat                                                                                                                      >  2:359454/1‑138 (MQ=255)
                         acaaaaGCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTg                                                                                                                  <  2:256923/138‑1 (MQ=255)
                           aaaaataTTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                <  2:274670/134‑1 (MQ=255)
                           aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                <  1:267580/140‑1 (MQ=255)
                           aaaaTCACTCCAACAAAAGTAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                <  1:278493/140‑1 (MQ=255)
                            aaaTTATTCTATCAAAAGAAATCAACGATCAAAAAATCAAAAAAGGATTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGATTTTATAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                               <  1:15838/140‑1 (MQ=17)
                            aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                               >  1:124396/1‑140 (MQ=255)
                               tCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                            <  1:306927/140‑1 (MQ=255)
                                          aaaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATATGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTaa                                                                                                 >  1:485955/1‑140 (MQ=255)
                                           aaGAAATTAAAGATGAAGAAATAAAAAAAGAAATCGAAAACCTTAATTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTaaa                                                                                                <  2:328966/140‑1 (MQ=255)
                                           aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTaaa                                                                                                <  2:601839/140‑1 (MQ=255)
                                           aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATTCGGCTaaa                                                                                                >  2:182931/1‑140 (MQ=255)
                                             gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATGATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                              <  1:1754/140‑1 (MQ=255)
                                                aTTAAAGATGACAAAATAAAAAAAAAAATCGAAAACTTTAAGTTCTTTGTTCAATATCGCGACATTAAAAATTTGAAAAATTATAACGACGGAGATATTTCAAATAATGAAGAGTAGCCGAGTTATTCGGCtcactagca                                                                                           >  2:33225/1‑132 (MQ=17)
                                                   aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                        >  2:97442/1‑140 (MQ=255)
                                                   aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                        >  2:460542/1‑140 (MQ=255)
                                                   aaaGATAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                        <  2:180169/140‑1 (MQ=255)
                                                    aagaataaaaaaTAAAAAAAGAAATCTAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                       <  2:321233/134‑1 (MQ=255)
                                                     ataTGAAAAAATAAAAAAAGAAATCGATAACTTTAAGTTCTTTGTTCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTGATTTTGCTAAATATCAATTaa                                                                                      <  2:144190/138‑1 (MQ=17)
                                                     aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTCGAAAAATCATCCCGACCGCGCTATTCCATATCCTCCAGCGCTCCCCAGTCACTCGGCTAACTCTCaaccaa                                                                                      >  2:139520/1‑136 (MQ=12)
                                                       aTGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                    <  1:106827/140‑1 (MQ=255)
                                                        agAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTGTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTCCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                   <  2:168978/139‑1 (MQ=255)
                                                          aaaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGAGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                 <  1:550340/140‑1 (MQ=255)
                                                             ataTAAAAAAAGAAATCGAAAACTTTAAGTTCTGTGTTCAATATGGCGTCTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                              <  1:317793/138‑1 (MQ=255)
                                                              aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                             <  2:234261/140‑1 (MQ=255)
                                                               aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATTTTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                            <  1:200981/140‑1 (MQ=255)
                                                                  aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                         >  2:580704/1‑140 (MQ=255)
                                                                     aaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGCAAAATTTCATATAATACAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTaaaa                                                                      >  1:155071/1‑140 (MQ=17)
                                                                     aaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATaa                                                                      >  1:542904/1‑140 (MQ=255)
                                                                      aaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                     >  1:641631/1‑140 (MQ=255)
                                                                       aGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                    <  1:643105/140‑1 (MQ=255)
                                                                         aacgcgatgaCTTTAAGTTCTTTTTTCAATATTGGGAGTTTAAAAATTTGAAAAATTATAAAGACGGATATATTTCATATAATCCAGAGGGGCCGAGTTATTCGGCTAAATATAAATTAACTAATGATGATTATAATGTa                                                                  <  1:102284/131‑1 (MQ=17)
                                                                         aaaTCGAAAACTTTCAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTGTAATGTa                                                                  <  1:416035/140‑1 (MQ=255)
                                                                         aaaTCGAAAACTTTAAGTTCTTTGTTCAATACGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                  <  2:16744/140‑1 (MQ=255)
                                                                                    tttAAGTTCTTTGTTCAATATGGTGACTTTAAAAATTTTAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGTTGCCGAGTTATTCGGCTAAATATCAATTAACTAAGGATGATTATAATGTAAAACAATTACg                                                       <  1:205390/140‑1 (MQ=255)
                                                                                        atttCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                   <  1:78548/138‑1 (MQ=255)
                                                                                                gTTCAATATGGCGGCTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                           >  1:662986/1‑140 (MQ=255)
                                                                                                         ggCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgac                                  >  2:591227/1‑140 (MQ=255)
                                                                                                          gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                 <  2:382504/140‑1 (MQ=255)
                                                                                                               tttAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACATCTATCCAAAAGATATGATATACCGACGAGTaa                            <  1:159177/140‑1 (MQ=255)
                                                                                                                 tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAGcgacgaacaaca                          >  2:327056/1‑134 (MQ=255)
                                                                                                                 tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAg                          >  2:228524/1‑140 (MQ=255)
                                                                                                                  aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                         >  2:199647/13‑140 (MQ=11)
                                                                                                                  aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                         >  2:644096/1‑140 (MQ=255)
                                                                                                                  aaaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                         <  1:255915/140‑1 (MQ=255)
                                                                                                                   aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCAACGAGTAAAGcc                        >  1:107342/12‑139 (MQ=11)
                                                                                                                   aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATAAAAGACCGACGAGTAAAGCt                        >  2:601539/12‑140 (MQ=11)
                                                                                                                    aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                       >  2:82103/1‑140 (MQ=255)
                                                                                                                     gatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAATATAATGTAAAACAATTACGCAAAAGAAAAGATATACCGACGAGAAAAGCTcc                      >  1:318467/10‑140 (MQ=11)
                                                                                                                     aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGATTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                      <  1:244433/140‑1 (MQ=255)
                                                                                                                     aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATAAACCGACGAGTAAAGCTcc                      >  1:285008/1‑140 (MQ=255)
                                                                                                                      aTTTGAAAAATCATAAAGACGTGTGTGCTTTATATAATCCAGAAGCGCCTAATTATTCGGCTAAACATCAATTAACTAATGATGATTACAATGTAAAACAATTACGCAAAAGATATGAGATACCGACGAGTAAAGCTCCa                     <  2:513341/140‑1 (MQ=17)
                                                                                                                       tttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaa                    >  2:509017/8‑140 (MQ=14)
                                                                                                                       tttGAAAATTTATTAAGACGGAGATATTTCATCTAATCCAGTGATGCCGATTTATTCGTCTAAATTTCAATTACCCTATTACGATTATGATGTAAAACAACTCCGCATTATGTATGATATAACGATGAGTAGAGCTCCat                    <  2:207072/140‑2 (MQ=12)
                                                                                                                            tgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGGCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa               <  1:655658/138‑1 (MQ=255)
                                                                                                                             gaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt              <  1:60097/139‑1 (MQ=255)
                                                                                                                             aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAGCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt              >  2:335105/1‑140 (MQ=255)
                                                                                                                                ttATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           <  1:527809/140‑1 (MQ=255)
                                                                                                                                ttATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           >  1:548564/1‑140 (MQ=255)
                                                                                                                                     aaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCTAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa      <  1:534995/140‑1 (MQ=255)
                                                                                                                                      aGACGGAGATATTTCATATAATCCAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg     <  1:510600/140‑1 (MQ=255)
                                                                                                                                      aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGGGTAAAGCTCCAAAGTTATTGTTAAAAg     >  2:445946/1‑140 (MQ=255)
                                                                                                                                         cGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt  <  2:124396/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
AGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  CP000730/110514‑110790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: