Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,607 T→C 20.6% D22G (GAT→GGT)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,6070TC20.6% 101.8 / 24.1 60D22G (GAT→GGT) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (25/21);  new base C (5/7);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

ATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAA  >  CP000730/1744468‑1744746
                                                                                                                                           |                                                                                                                                           
aTAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCATCATTATCCATAATTGTAGTAatat                                                                                                                                             <  1:485582/140‑1 (MQ=255)
              aGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAaca                                                                                                                               <  1:217951/140‑1 (MQ=255)
               gCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTATCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAacac                                                                                                                              >  2:181571/1‑140 (MQ=255)
                ccAATGTTAATGCCACGCGCTTCAGGTCCTACACCGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACAcc                                                                                                                             <  2:520834/140‑1 (MQ=255)
                  actGATAAAGCCACCCGCTTCAGTGCCTACAACTTTATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATATTTGTAGTAATAGCGGGGAAACTAACACCTg                                                                                                                           <  2:240297/138‑1 (MQ=255)
                    tGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCATTGAAACTAACACCTGGt                                                                                                                         >  2:302160/1‑140 (MQ=255)
                         aaGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCTTATTCTACAATTTTATCTGTTTCATAGCCATATACTACACCATTATCAATAATTGTAATAATATCCTTATAACTAACACCTGATTTcag                                                                                                                    >  1:62434/1‑138 (MQ=255)
                         aaGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTACATAGCCATATGCTTTACCATTATCCATAATTGTAGTAATATCCTTGAAATTAACACCTGttttatg                                                                                                                    >  1:256450/1‑137 (MQ=255)
                           gCCACGCGATTCAGGGCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTGCAGTTGTGTCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGcc                                                                                                                  >  2:489336/1‑140 (MQ=255)
                               cgcgCTTCAGGTCCTACAATGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGTTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATc                                                                                                              <  1:400677/140‑1 (MQ=255)
                               cgcgCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATc                                                                                                              <  1:633749/140‑1 (MQ=255)
                                gcccTTCAGGTCCTACAACGATATCCACATCCCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCt                                                                                                             <  2:382925/137‑1 (MQ=255)
                                               cAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACg                                                                                              <  1:364046/140‑1 (MQ=255)
                                               cAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACg                                                                                              <  1:282290/140‑1 (MQ=255)
                                                 acgaagtCAATATATCTGTATTTTGCGTATTCTACAATTTTATCTTTTGCATACCCATATGTTTAACCATTATCAATAATTATAGTATTAACCTTGAAACTAACACCTGGTTTCGGCCAAGATTGGACTTCTGATACGTa                                                                                            >  2:225893/7‑140 (MQ=255)
                                                 aCGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTACCACCTGGTTTCGGCCAATCTTGAACTTATGATCCGTa                                                                                            >  2:208383/1‑140 (MQ=255)
                                                 aCGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTa                                                                                            >  2:500696/1‑140 (MQ=255)
                                                  cGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATCCGTAt                                                                                           >  2:651961/1‑140 (MQ=255)
                                                  cGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGCTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTAt                                                                                           >  2:400765/1‑140 (MQ=255)
                                                  cGATATCAACATCTCTGTCTTTTGCGTATTCTACAAATTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTTAAGCTACCACCTGTTTTAGCCCAATCTTAAACTTCTGATACGTAt                                                                                           >  2:245274/1‑140 (MQ=255)
                                                     tatCAACATGTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATGCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGc                                                                                        <  1:117706/140‑1 (MQ=255)
                                                       tCACCATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCAGATGCTTCACCACTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCtt                                                                                      <  2:44434/140‑1 (MQ=255)
                                                       tCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGACACTAAGAGGTGGTATCTGCCAATCTTGAACTTCTGATACGTATTTCtt                                                                                      >  1:513616/1‑140 (MQ=255)
                                                            aTCTCTGTCTTTTGCGTCTTTTACAATTTTATCTGTTGCATGGCCCTATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTTTGATACGTCTTGCGTTCAAt                                                                                 >  1:592128/1‑140 (MQ=255)
                                                                  gTCTTTTGCGTATTCTACAATTTTATCTGTTGCAGAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTa                                                                           <  2:62706/140‑1 (MQ=255)
                                                                          cGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTtcc                                                                   >  2:148744/1‑140 (MQ=255)
                                                                                  aCAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAAt                                                           <  2:112182/140‑1 (MQ=255)
                                                                                  aCAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAAt                                                           <  1:134621/140‑1 (MQ=255)
                                                                                      ttttttCTGTTGCATAGCCATATGCTTCACCATTATCCTTAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCt                                                       <  1:118415/140‑1 (MQ=255)
                                                                                          aTCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACg                                                   >  1:169486/1‑140 (MQ=255)
                                                                                           tCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCCTTAATATTTCCTCCTAAATTGCTCACGa                                                  >  2:656082/1‑140 (MQ=255)
                                                                                           tCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTATATTGCGCACGa                                                  <  2:407871/140‑1 (MQ=255)
                                                                                           tCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGa                                                  <  1:364295/140‑1 (MQ=255)
                                                                                           tCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGa                                                  <  1:295819/140‑1 (MQ=255)
                                                                                           tCTGTTGCATAGCCATATCCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGa                                                  <  1:643414/140‑1 (MQ=255)
                                                                                            cTGTTGCATAGCCATATGCTGCACCATTATCCATAATTGTAGTAGTATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCATGAc                                                 >  1:405675/1‑140 (MQ=255)
                                                                                             tGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACa                                                <  2:204234/140‑1 (MQ=255)
                                                                                             tGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACa                                                <  2:115743/140‑1 (MQ=255)
                                                                                              gTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACaa                                               >  2:560220/1‑140 (MQ=255)
                                                                                               ttGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGGCAATCTTGAACTTCTGATACGTATTACTTTAAGTCCATTAATATTTCGTCCTAAATGGCTAACGACAAt                                              >  2:516940/1‑140 (MQ=255)
                                                                                                  cATAGCCCTATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATtgt                                           <  2:62434/140‑1 (MQ=255)
                                                                                                   aTAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAGTTGCTCAAGCCACTtgtg                                          >  2:87687/1‑140 (MQ=255)
                                                                                                   aTAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGATTTCGGCCAATCTTGAAATTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATtgtg                                          >  1:590244/1‑140 (MQ=255)
                                                                                                       ccATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCGCACGACAATTGTGACtt                                      >  2:209308/1‑140 (MQ=255)
                                                                                                        cATATGCTTCACCATTATCGATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTGGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACttt                                     >  1:399912/1‑140 (MQ=255)
                                                                                                          tatGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTAt                                   >  1:606250/1‑140 (MQ=255)
                                                                                                          tatGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTAACTTTAt                                   >  1:606094/1‑140 (MQ=255)
                                                                                                            tGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAGTTGTGACTTTAtgc                                 >  1:498111/1‑138 (MQ=255)
                                                                                                            tGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATcc                                 >  1:600416/1‑140 (MQ=255)
                                                                                                              cTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCaa                               >  1:14044/1‑140 (MQ=255)
                                                                                                                 cACCATTTTCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAttt                            <  2:256450/140‑1 (MQ=255)
                                                                                                                     aTTATCCATAATTGTAGTTATATCCTTGAATCTAACACCTGGTTTCGGCCAATCTTGATCTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTa                        <  1:298509/140‑1 (MQ=255)
                                                                                                                      ttATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATATTGAACTTCTAAGACGTATTGCTTTAAATACATTAATATTTCCTCCTACATTGCTAACGACCACTGTACCTTTATCCAAtttattgt                       >  2:664146/1‑138 (MQ=255)
                                                                                                                      tcaTCCATAATTGTAGTAATATCCTTGCAACTAACACCTGGTTTCGGCCAATCTTGGACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACTACAATTGTGACTTTATCCAATTTTTTAt                       <  2:448619/138‑1 (MQ=255)
                                                                                                                        aTCCTTAATTCTAGTAATCTCCTTGAAACTAACACCTGGGTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTAttt                     <  1:650033/140‑1 (MQ=255)
                                                                                                                          ccATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTTTTTCt                   >  2:343499/1‑140 (MQ=255)
                                                                                                                           cATAATTGTAGTAATACCCTTGAATCTAACACCTGGTTTCGGCCAATCTTGATCTTCTGATACGTATTGCTTTAAATCCATTATTTTTTCCTCCTAAATTGCTCAGGACAATTGTGACTTTATCCAATTTTTTATTTCTg                  <  1:70945/140‑1 (MQ=255)
                                                                                                                                  gtagtaATATCCTTGAATCTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTTCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATc           <  1:101342/140‑1 (MQ=255)
                                                                                                                                    agtaATATCCTTTAAACTAACACCTTGTTTCGCACAATCTTGAACTTCTGATACGTACTGCTTTAAATCCATTAATATTTCCTCATAAACTGCTCACGACAATTTTCACTTTATCCAATTTTTCATTTCTGAAAAAGCtt         <  2:389043/140‑1 (MQ=255)
                                                                                                                                     gtaATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTg        >  1:17077/1‑140 (MQ=255)
                                                                                                                                       aaTATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGat      <  1:400765/140‑1 (MQ=255)
                                                                                                                                         tatCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGatat    <  2:14044/140‑1 (MQ=255)
                                                                                                                                           tCCCTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATGAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTTTGAAAAATCTGGATataa  >  2:6896/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTGATATAA  >  CP000730/1744468‑1744746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: